LOCUS       LC744459               10494 bp    DNA     circular BCT 24-DEC-2022
DEFINITION  Escherichia coli MyNCGM205 plasmid pMyNCGM205 DNA, complete
            sequence.
ACCESSION   LC744459
VERSION     LC744459.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Complete plasmid sequence of multidrug-resistant Escherichia coli
            co-harboring blaNDM-5 and rmtB in Myanmar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2022) Contact:Tomomi Hishinuma Juntendo University
            School of Medicine, Department of Microbiology; 2-1-1 Hongo,
            Bunkyo-ku, Tokyo 113-8421, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Sequencing Technology :: MinION; Illumina MiSeq
            ##Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MyNCGM205"
                     /db_xref="taxon:562"
                     /plasmid="pMyNCGM205"
                     /geo_loc_name="Myanmar"
                     /collection_date="2016"
     gene            1..348
                     /gene="ebr"
     CDS             1..348
                     /gene="ebr"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000679427.1"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="BDV15564.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="ebr"
     CDS             342..1181
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3ERE4"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="BDV15565.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGLDHA"
                     /name="dihydropteroate synthase"
     CDS             1586..3127
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001120888.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="BDV15566.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91 family transposase"
     CDS             3440..4471
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV15567.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSAQ
                     ATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYRKE
                     LLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKARGL
                     HDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRVQGTE
                     GLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHA
                     FVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALN
                     DAY"
                     /name="hypothetical protein"
     gene            complement(4482..5120)
                     /gene="trpF"
     CDS             complement(4482..5120)
                     /gene="trpF"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:E8TBD8"
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="BDV15568.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="trpF"
     CDS             complement(5125..5490)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010920626.1"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="BDV15569.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQL
                     EFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLRIG
                     YLIDPDCTLVRLIQNPD"
                     /name="bleomycin resistance protein"
     gene            complement(5494..6306)
                     /gene="bla"
     CDS             complement(5494..6306)
                     /gene="bla"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q6HFY5"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="BDV15570.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="bla"
     CDS             complement(6407..6601)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV15571.1"
                     /translation="MNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPLE
                     KFAQLVDYHMAFETVAPHV"
                     /name="hypothetical protein"
     CDS             6847..7569
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001313567.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDV15572.1"
                     /translation="MLMELHMNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERG
                     VNVDHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR
                     GRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCP
                     SDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAF
                     YYGDPLGEMRLVSRVFEM"
                     /name="IS6 family transposase"
     CDS             complement(7603..8490)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000845048.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="BDV15573.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRRALLQS"
                     /name="integron integrase"
     CDS             8635..9132
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3MV56"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="BDV15574.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGKV
                     VVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVAGG
                     AEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVYARR
                     NG"
                     /name="dihydrofolate reductase"
     CDS             9540..10331
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3EQ55"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin 3''-adenylyltransferase"
                     /protein_id="BDV15575.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPY
                     SDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLF
                     EALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
                     /name="streptomycin 3''-adenylyltransferase"
     ARG             2..345
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             343..1178
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5498..6306)
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9541..10328
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8636..9129
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5129..5490)
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9553..10331
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5126..5490)
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatccgca
       61 ttaaaatcta gcgagggctt tactaagctt gccccttccg ccgttgtcat aatcggttat
      121 ggcatcgcat tttattttct ttctctggtt ctgaaatcca tccctgtcgg tgttgcttat
      181 gcagtctggt cgggactcgg cgtcgtcata attacagcca ttgcctggtt gcttcatggg
      241 caaaagcttg atgcgtgggg ctttgtaggt atggggctca taattgctgc ctttttgctc
      301 gcccgatccc catcgtggaa gtcgctgcgg aggccgacgc catggtgacg gtgttcggca
      361 ttctgaatct caccgaggac tccttcttcg atgagagccg gcggctagac cccgccggcg
      421 ctgtcaccgc ggcgatcgaa atgctgcgag tcggatcaga cgtcgtggat gtcggaccgg
      481 ccgccagcca tccggacgcg aggcctgtat cgccggccga tgagatcaga cgtattgcgc
      541 cgctcttaga cgccctgtcc gatcagatgc accgtgtttc aatcgacagc ttccaaccgg
      601 aaacccagcg ctatgcgctc aagcgcggcg tgggctacct gaacgatatc caaggatttc
      661 ctgaccctgc gctctatccc gatattgctg aggcggactg caggctggtg gttatgcact
      721 cagcgcagcg ggatggcatc gccacccgca ccggtcacct tcgacccgaa gacgcgctcg
      781 acgagattgt gcggttcttc gaggcgcggg tttccgcctt gcgacggagc ggggtcgctg
      841 ccgaccggct catcctcgat ccggggatgg gatttttctt gagccccgca ccggaaacat
      901 cgctgcacgt gctgtcgaac cttcaaaagc tgaagtcggc gttggggctt ccgctattgg
      961 tctcggtgtc gcggaaatcc ttcttgggcg ccaccgttgg ccttcctgta aaggatctgg
     1021 gtccagcgag ccttgcggcg gaacttcacg cgatcggcaa tggcgctgac tacgtccgca
     1081 cccacgcgcc tggagatctg cgaagcgcaa tcaccttctc ggaaaccctc gcgaaatttc
     1141 gcagtcgcga cgccagagac cgagggttag atcatgccta gcattcacct tccggccgcc
     1201 cgctaaatat ctccttttgg gttgttaata aaacatccaa taagttgact gtgcgtgaaa
     1261 aagaaagttt tgtgtgatgg cgttgaagat cgcaccgtta agctcttatg tgggatggtg
     1321 cagagctcga cgactaccga taaaacgcaa ccgccgcaaa cagacaagaa aaagccccaa
     1381 ctgataacag ttggggcttc agtattgtga ttggtggagc aatagcaccc tgaacccaaa
     1441 accttctcgc tcaaccggta gtggctgata acaactcgtg agggctattg cgggttaagc
     1501 atttagcgat gtctagggcc agactggacg tctgaacgca agccgctgat actgtacata
     1561 accacagtat cagcggagga tacccatgtc gctggcaagg aacgccacgg cgagtcaatc
     1621 gcccactcaa acaaacggtt acgaacgcca ccaacccgac cagacgctgc tctaccagct
     1681 ggttgagcag cactacccag ccttcaaagc ctcactcgaa gcccaaggtc aacacctgcc
     1741 tcgctacatc caacaagaat tcaacgacct cctccaatgt ggccgtctgg agtatggttt
     1801 catgcgggtt cgctgcgagg attgtcatca cgagcgtctg gtcgccttca gctgtaaacg
     1861 acgcggcttt tgccctagct gcggtgcccg ccggatggcc gagagtgcgg cgctgctgat
     1921 agacgaagtc ttccccaagg agcccattcg ccagtgggtg ctcagctttc ctttccagct
     1981 acgctttttg ctggctcgcc atccccagct gatgggccag gtcttgagta tcgtctatcg
     2041 tacactctca actcatctga tcaaaaaagc cggttacacc aaagcctctg cacaaactgg
     2101 ctcagtgact cttatccaac gctttggctc cgcgctaaat ctcaatgtcc actaccacat
     2161 gctgtttctc gatggtgtct atgccgaaga tgactatggc aagcaacgct tccatcgtgt
     2221 caaggcaccc acttacgatg agctgaatac gctcgctcac accctcagcc atcgcatcgc
     2281 tcgctgcatg gaaaagcgtg ggattttgga gcgtgatgcc gagaatacgt ggttgacact
     2341 ggaagagggc gaagacgata cgctgactca attacatggt gcttcggtta cgtatcgcat
     2401 tgccgtcggc ccccagcaag ggcgcaaagt cttcaccctg caaaccttgc cagggcgtga
     2461 ggataaagcc gactcaagca gtcgagtagc caaccatgct ggtttctcgc tacacgccgg
     2521 tgtgatggcc gaagcgcatc agcgggataa gcttgagcgc ttgtgtcgct acattagtcg
     2581 gccagcggtt tcagaaaaac gtctggcatt aaccgccaat gggcaggtgc gttacgagct
     2641 caaaactccg taccgcaatg gcaccaccca tgtgatcttc gagccgctgg acttcatcgc
     2701 caaactcgct gcgttggtac ctaagccgcg agtcaacctc acacgcttcc acggcgtctt
     2761 tgcaccgaac agcaaacacc gagttcaagt aacacccgcc aagcggggca agaagcccga
     2821 caaatcggaa ggtctcgata ctaactggcg tgacaagagt cctgcagagc gccaccgcgc
     2881 catgacctgg atgcaacgcc tcaagcgagt cttcaatatt gatattgaag tctgcgaaca
     2941 ctgcggcggt cacgtcaaag tgattgccag catcgaagat ccgaaggtca ttgagcagat
     3001 tctcaagcat ctgaaacaga aaacagccaa ggcgaatgcc gccaagcagc gtgagctgcc
     3061 accagaacga gcgccgccac tgactcccag cctgttcgat ccatcacaga gtcgtctctt
     3121 tgactgacga ccccaaatcc aacactgctc aacactgcca acttttaaac ggggcggtgg
     3181 ggcagtttgt atctctcgag ctatcaggct agagatttta ccgccaaatc gaaccttatt
     3241 agagcggttt aggctggacc ggcagttaaa attggggctt gagcggtaaa cgagtgaggg
     3301 aatttcaggt aagatacttc ggatgaggag caaaaaggtg gtttatactt cctatacccg
     3361 ctcgcggtcg aagccgcgtc cggcctgccc gaatacctgc aatggctggc cgccgagagc
     3421 cgaccggtaa actgagccaa tgaccgcatc cacgatccgc ctgcgccgct ggctggccgg
     3481 gctcgccctg ctgcttgcgc tgcccgcgac ctcggccgtc gcccaggact tcgaactgcc
     3541 gccggtcgac gaggtcttcg tcctgtccgc gcaggccacc gcgccggacc gcatcgaggt
     3601 gcgctggcgg atcgccgacg gctattacct gtaccggcac cgcacctcgg tcaaggccga
     3661 tgccgccttc accggcgcga ccatggcgct gccgaagggc aaggcctacc gcgacgaatt
     3721 cttcggcgac gtcgaaacct accgcaagga attgctcggc accctcaccg gcacgcccgc
     3781 ggccggcgcg agcgcgacca ccctgaccgt gaagtaccag ggctgcgccg atgccggcgt
     3841 gtgctacccg ccgcagaccc gcaccctgaa ggtcgcgttg ccgggcgaag cgggcgctgg
     3901 cggcttcggc tgtaaggcgc gcgggctgca tgattatgtc gtcaagaacg gctctgccga
     3961 tcatcccaac gccgaggtga aattcgcact gggtgatgtg gtcaacacca tgatcggctg
     4021 cactaatggt gaaacgatca tgctgtgcca cgacacctcg ctgccgcgcc cctattctct
     4081 cggctttcgg gtgcaaggca ccgaggggct gtggatggac gtcaacaagt cgatctatct
     4141 ggagggcaag agcccacagc cgcaccgctg ggagcctgcc gagggctggt ttgcgaaata
     4201 cgatcacccg ctatggaaac gctacgccga tctggcggca ggggccgggc atggcgggat
     4261 ggactggttc gtgatccacg cttttgtcga ggcgctgaag gccaaggccc cgatgccaat
     4321 cgacatttac gacgcgctgg cctggagcgc gatcacgcct ctgtcggaac aatcgattgc
     4381 tgagggcaat cgcacgttag attttcccga cttcacccga gggcagtggc gcacccgcaa
     4441 gccgatcttt gcgctgaacg acgcctattg atcgacgcga tttaggccaa gcgcaccgca
     4501 aaggcgaaat tggtaatctt gtcggtatcc ttgacgcccg gcgcgctttc gacgccgctg
     4561 gaggtatcga ccagcggcgc tccggtgcgc gcaatcgcct cggcaacatt cgtcggattt
     4621 agcccgcctg ccagccccca cggcaaggca ccgcgatatc cggccagcag cgaccagtcg
     4681 aacgccaacc ccatgccgcc gggcagcgcg cctttggggg tcttggcgtc gaacaagatc
     4741 aagtccgccg ccccggcata ggctgcggcg cgtgcgacat cgctggcgct ggcgacgggc
     4801 agcgccttcc acaccggctt gccaaaccgc gcgcgcaact gggccacgcg ttcgggcgat
     4861 tccgaaccgt gcagctgcag cgcgttcagc ttggctgcca ccagtgcgtc ggcgatgaca
     4921 gcatcatccg catcgacgaa caaaccgacc atggcgatct ggccagctgc gcgcgatgtc
     4981 aaagcgcccg cgacattcga cgtaaccgca cggggcgacg ctggatagaa caccaacccg
     5041 gcatagtccg cccgcgccgc gatggtcgca tcgagcgcct cgggtgtgct gatcccgcaa
     5101 atcttgattt tcgcgggcat gcggtcagtc ggggttctgg atcagccgca ccagcgtgca
     5161 gtcgggatcg atcaggtagc cgatcctcag gccgctcgcc tccagttgcg gagctttgaa
     5221 gcgcggccag ccggtgcttt tttcctcggc tcccgccgcg ttcaccaatg ccaccatggc
     5281 atcgagatca tccaaccgca ggcaacagcc gaacgagctc gtagctgggt cgaggtcagg
     5341 ataggggaag aattcgagct gcaaaccgcc gcgctgcagg atcatccagc cgcgatcctt
     5401 ccaactcgtc gcaaagccca gcttcgcata aaacgcctct gtcacatcga aatcgcgcga
     5461 tggcagattg ggggtgacgt ggtcagccat ggctcagcgc agcttgtcgg ccatgcgggc
     5521 cgtatgagtg attgcggcgc ggctatcggg ggcggaatgg ctcatcacga tcatgctggc
     5581 cttggggaac gccgcaccaa acgcgcgcgc tgacgcggcg tagtgctcag tgtcggcatc
     5641 accgagattg ccgagcgact tggccttgct gtccttgatc aggcagccac caaaagcgat
     5701 gtcggtgccg tcgatcccaa cggtgatatt gtcactggtg tggccggggc cggggtaaaa
     5761 taccttgagc gggccaaagt tgggcgcggt tgctggttcg acccagccat tggcggcgaa
     5821 agtcaggctg tgttgcgccg caaccagccc ctcttgcggg gcaagctggt tcgacaacgc
     5881 attggcataa gtcgcaatcc ccgccgcatg cagcgcgtcc ataccgccca tcttgtcctg
     5941 atgcgcgtga gtcaccaccg ccagcgcgac cggcaggttg atctcctgct tgatccagtt
     6001 gaggatctgg gcggtctggt catcggtcca ggcggtatcg accaacagca cgcggccgcc
     6061 atccctgacg atcaaaccgt tggaagcgac tgccccgaaa cccggcatgt cgagatagga
     6121 agtgtgctgc cagacattcg gtgcgagctg gcggaaaacc agatcgccaa accgttggtc
     6181 gccagtttcc atttgctggc caatcgtcgg gcggatttca ccgggcatgc acccgctcag
     6241 catcaatgca gcggctaatg cggtgctcag cttcgcgacc gggtgcataa tattgggcaa
     6301 ttccatcaag ttttcctttt attcagcatt aaaaaccccg caaatgcgag gcctagtaaa
     6361 tagatgatct taatttggtt cactgtagca aaaatatggg gcgaattcaa acatgaggtg
     6421 cgacagtttc aaaagccata tgataatcaa caagctgagc aaatttctct aatggtgtaa
     6481 gccaatctaa cgcctttcta ggacgagtat tcagtgacat ggcaacttga tttaaataat
     6541 gctgatctgc ctgatttaaa tcaatccctt taggtaaata ttgcctaatt aaaccattca
     6601 tattttcgca tgtgcctttt tgccagggtg aatgtgggtc acagaaatat acatctatgc
     6661 ctaaatcttc ttcgagtatt ttatgttgag cacttttctt tcacccaatg ggtgaaagct
     6721 gaatttcgaa ggaatgcata tttatcaagg ctttgattat gctttttgaa gtactgacgt
     6781 agaatctagg tatgagtaaa cggcactgtt gcaaatagtc ggtggtgata aacttatcat
     6841 ccccttttgc tgatggagct gcacatgaac ccattcaaag gccggcattt tcagcgtgac
     6901 atcattctgt gggccgtacg ctggtactgc aaatacggca tcagttaccg tgagctgcag
     6961 gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca cgatttaccg ctgggttcag
     7021 cgttatgcgc ctgaaatgga aaaacggctg cgctggtact ggcgtaaccc ttccgatctt
     7081 tgcccgtggc acatggatga aacctacgtg aaggtcaatg gccgctgggc gtatctgtac
     7141 cgggccgtcg acagccgggg ccgcactgtc gatttttatc tctcctcccg tcgtaacagc
     7201 aaagctgcat accggtttct gggtaaaatc ctcaacaacg tgaagaagtg gcagatcccg
     7261 cgattcatca acacggataa agcgcccgcc tatggtcgcg cgcttgctct gctcaaacgc
     7321 gaaggccggt gcccgtctga cgttgaacac cgacagatta agtaccggaa caacgtgatt
     7381 gaatgcgatc atggcaaact gaaacggata atcggcgcca cgctgggatt taaatccatg
     7441 aagacggctt acgccaccat caaaggtatt gaggtgatgc gtgcactacg caaaggccag
     7501 gcctcagcat tttattatgg tgatcccctg ggcgaaatgc gcctggtaag cagagttttt
     7561 gaaatgtaag gcctttgaat aagacaaaag gctgcctcat cgctaacttt gcaacagtgc
     7621 cctgcggagc aaggccgtcg cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt
     7681 gatgcctgct tgttctacgg cacgtttgaa ggcgcgctga aaggtctggt catacatgtg
     7741 atggcgacgc acgacaccgc tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca
     7801 gaaccacggc caggaatgcc cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc
     7861 aacgccgctg cggccctcgg cctggtcctt cagccaccat gcccgtgcac gcgacagctg
     7921 ctcgcgcagg ctgggtgcca agctctcggg taacatcaag gcccgatcct tggagccctt
     7981 gccctcccgc acgatgatcg tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa
     8041 accctcactg atccgcatgc ccgttccata cagaagctgg gcgaacaaac gatgctcgcc
     8101 ttccagaaaa ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg
     8161 cgacggccga ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc
     8221 gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc
     8281 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
     8341 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttggtaa
     8401 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     8461 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
     8521 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     8581 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag ccatatgaac
     8641 tcggaatcag tacgcattta tctcgttgct gcgatgggag ccaatcgggt tattggcaat
     8701 ggtcctaata tcccctggaa aattccgggt gagcagaaga tttttcgcag actcactgag
     8761 ggaaaagtcg ttgtcatggg gcgaaagacc tttgagtcta tcggcaagcc tctaccgaac
     8821 cgtcacacat tggtaatctc acgccaagct aactaccgcg ccactggctg cgtagttgtt
     8881 tcaacgctgt cgcacgctat cgctttggca tccgaactcg gcaatgaact ctacgtcgcg
     8941 ggcggagctg agatatacac tctggcacta cctcacgccc acggcgtgtt tctatctgag
     9001 gtacatcaaa ccttcgaggg tgacgccttc ttcccaatgc tcaacgaaac agaattcgag
     9061 cttgtctcaa ccgaaaccat tcaagctgta attccgtaca cccactccgt ttatgcgcgt
     9121 cgaaacggct aaccattccg tcaacgggac gccaaaatgc tgcgcatttt ggttccctcc
     9181 gctgcgctcc ggctctcgtt acgtccaacg ttagcaccac ttaaacccag ctttatttag
     9241 ctcatgttta ttcaaacggc atttagcttt tcaggcgtta ttcagtgcct gttttgcctt
     9301 ttttccgggc ttcgcctgca tgggctgcgc aggttttcag tctttttggc ctctagccct
     9361 tgcgtagcaa gcgcaagcag ctatcgtttt tgcagtgctg tgccgcctcg gtggcgcagc
     9421 gttttttcac ggttagcgcc cgtcgccaaa ttcaagttat ccgttttggc ttctggttct
     9481 aacatttcgg tcaagccgac ccgcattctg cggtcggctt acctcgcccg ttagacatca
     9541 tgagggaagc ggtgaccatc gaaatttcga accaactatc agaggtgcta agcgtcattg
     9601 agcgccatct ggaatcaacg ttgctggccg tgcatttgta cggctccgca gtggatggcg
     9661 gcctgaagcc atacagcgat attgatttgt tggttactgt ggccgtaaag cttgatgaaa
     9721 cgacgcggcg agcattgctc aatgatctta tggaggcttc ggctttccct ggcgagagcg
     9781 agacgctccg cgctatagaa gtcacccttg tcgtgcatga cgacatcatc ccgtggcgtt
     9841 atccggctaa gcgcgagctg caatttggag aatggcagcg caatgacatt cttgcgggta
     9901 tcttcgagcc agccatgatc gacattgatc tagctatcct gcttacaaaa gcaagagaac
     9961 atagcgttgc cttggtaggt ccggcagcgg aggaattctt tgacccggtt cctgaacagg
    10021 atctattcga ggcgctgagg gaaaccttga agctatggaa ctcgcagccc gactgggccg
    10081 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca ataaccggca
    10141 aaatcgcgcc gaaggatgtc gctgccgact gggcaataaa acgcctacct gcccagtatc
    10201 agcccgtctt acttgaagct aagcaagctt atctgggaca aaaagaagat cacttggcct
    10261 cacgcgcaga tcacttggaa gaatttattc gctttgtgaa aggcgagatc atcaagtcag
    10321 ttggtaaatg atgtctaaca attcgttcaa gccgaccgcg ctacgcgcgg cggcttaact
    10381 ccggcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
    10441 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatc
//
