LOCUS       LC744442               10494 bp    DNA     circular BCT 24-DEC-2022
DEFINITION  Escherichia coli MyJU800 plasmid pMyJU800 DNA, complete sequence.
ACCESSION   LC744442
VERSION     LC744442.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Complete plasmid sequence of multidrug-resistant Escherichia coli
            co-harboring blaNDM-5 and rmtB in Myanmar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2022) Contact:Tomomi Hishinuma Juntendo University
            School of Medicine, Department of Microbiology; 2-1-1 Hongo,
            Bunkyo-ku, Tokyo 113-8421, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Sequencing Technology :: MinION; Illumina MiSeq
            ##Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MyJU800"
                     /db_xref="taxon:562"
                     /plasmid="pMyJU800"
                     /geo_loc_name="Myanmar"
                     /collection_date="2018"
     gene            330..677
                     /gene="ebr"
     CDS             330..677
                     /gene="ebr"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000679427.1"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="BDV13280.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="ebr"
     CDS             671..1510
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3ERE4"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="BDV13281.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGLDHA"
                     /name="dihydropteroate synthase"
     CDS             1915..3456
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001120888.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="BDV13282.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91 family transposase"
     CDS             3769..4800
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV13283.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSAQ
                     ATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYRKE
                     LLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKARGL
                     HDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRVQGTE
                     GLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHA
                     FVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALN
                     DAY"
                     /name="hypothetical protein"
     gene            complement(4811..5449)
                     /gene="trpF"
     CDS             complement(4811..5449)
                     /gene="trpF"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:E8TBD8"
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="BDV13284.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="trpF"
     CDS             complement(5454..5819)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010920626.1"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="BDV13285.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQL
                     EFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLRIG
                     YLIDPDCTLVRLIQNPD"
                     /name="bleomycin resistance protein"
     gene            complement(5823..6635)
                     /gene="bla"
     CDS             complement(5823..6635)
                     /gene="bla"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q6HFY5"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="BDV13286.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="bla"
     CDS             complement(6736..6930)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV13287.1"
                     /translation="MNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPLE
                     KFAQLVDYHMAFETVAPHV"
                     /name="hypothetical protein"
     CDS             7176..7898
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001313567.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDV13288.1"
                     /translation="MLMELHMNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERG
                     VNVDHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR
                     GRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCP
                     SDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAF
                     YYGDPLGEMRLVSRVFEM"
                     /name="IS6 family transposase"
     CDS             complement(7932..8819)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000845048.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="BDV13289.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRRALLQS"
                     /name="integron integrase"
     CDS             8964..9461
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3MV56"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="BDV13290.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGKV
                     VVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVAGG
                     AEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVYARR
                     NG"
                     /name="dihydrofolate reductase"
     ARG             331..674
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             672..1507
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5827..6635)
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8965..9458
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5458..5819)
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3..166
                     /name="aadA12"
                     /gene="aadA12"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="20.53"
     ARG             9882..10492
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="78.76"
     ARG             complement(5455..5819)
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 cgactgggca ataaaacgcc tacctgccca gtatcagccc gtcttacttg aagctaagca
       61 agcttatctg ggacaaaaag aagatcactt ggcctcacgc gcagatcact tggaagaatt
      121 tattcgcttt gtgaaaggcg agatcatcaa gtcagttggt aaatgatgtc taacaattcg
      181 ttcaagccga ccgcgctacg cgcggcggct taactccggc gttagatgca ctaagcacat
      241 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
      301 taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa
      361 tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt actaagcttg
      421 ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt tctctggttc
      481 tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc gtcgtcataa
      541 ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc tttgtaggta
      601 tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag tcgctgcgga
      661 ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga
      721 tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt
      781 cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga ggcctgtatc
      841 gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg atcagatgca
      901 ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca agcgcggcgt
      961 gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg atattgctga
     1021 ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg ccacccgcac
     1081 cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg aggcgcgggt
     1141 ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc cggggatggg
     1201 atttttcttg agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct
     1261 gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc
     1321 caccgttggc cttcctgtaa aggatctggg tccagcgagc cttgcggcgg aacttcacgc
     1381 gatcggcaat ggcgctgact acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat
     1441 caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac gccagagacc gagggttaga
     1501 tcatgcctag cattcacctt ccggccgccc gctaaatatc tccttttggg ttgttaataa
     1561 aacatccaat aagttgactg tgcgtgaaaa agaaagtttt gtgtgatggc gttgaagatc
     1621 gcaccgttaa gctcttatgt gggatggtgc agagctcgac gactaccgat aaaacgcaac
     1681 cgccgcaaac agacaagaaa aagccccaac tgataacagt tggggcttca gtattgtgat
     1741 tggtggagca atagcaccct gaacccaaaa ccttctcgct caaccggtag tggctgataa
     1801 caactcgtga gggctattgc gggttaagca tttagcgatg tctagggcca gactggacgt
     1861 ctgaacgcaa gccgctgata ctgtacataa ccacagtatc agcggaggat acccatgtcg
     1921 ctggcaagga acgccacggc gagtcaatcg cccactcaaa caaacggtta cgaacgccac
     1981 caacccgacc agacgctgct ctaccagctg gttgagcagc actacccagc cttcaaagcc
     2041 tcactcgaag cccaaggtca acacctgcct cgctacatcc aacaagaatt caacgacctc
     2101 ctccaatgtg gccgtctgga gtatggtttc atgcgggttc gctgcgagga ttgtcatcac
     2161 gagcgtctgg tcgccttcag ctgtaaacga cgcggctttt gccctagctg cggtgcccgc
     2221 cggatggccg agagtgcggc gctgctgata gacgaagtct tccccaagga gcccattcgc
     2281 cagtgggtgc tcagctttcc tttccagcta cgctttttgc tggctcgcca tccccagctg
     2341 atgggccagg tcttgagtat cgtctatcgt acactctcaa ctcatctgat caaaaaagcc
     2401 ggttacacca aagcctctgc acaaactggc tcagtgactc ttatccaacg ctttggctcc
     2461 gcgctaaatc tcaatgtcca ctaccacatg ctgtttctcg atggtgtcta tgccgaagat
     2521 gactatggca agcaacgctt ccatcgtgtc aaggcaccca cttacgatga gctgaatacg
     2581 ctcgctcaca ccctcagcca tcgcatcgct cgctgcatgg aaaagcgtgg gattttggag
     2641 cgtgatgccg agaatacgtg gttgacactg gaagagggcg aagacgatac gctgactcaa
     2701 ttacatggtg cttcggttac gtatcgcatt gccgtcggcc cccagcaagg gcgcaaagtc
     2761 ttcaccctgc aaaccttgcc agggcgtgag gataaagccg actcaagcag tcgagtagcc
     2821 aaccatgctg gtttctcgct acacgccggt gtgatggccg aagcgcatca gcgggataag
     2881 cttgagcgct tgtgtcgcta cattagtcgg ccagcggttt cagaaaaacg tctggcatta
     2941 accgccaatg ggcaggtgcg ttacgagctc aaaactccgt accgcaatgg caccacccat
     3001 gtgatcttcg agccgctgga cttcatcgcc aaactcgctg cgttggtacc taagccgcga
     3061 gtcaacctca cacgcttcca cggcgtcttt gcaccgaaca gcaaacaccg agttcaagta
     3121 acacccgcca agcggggcaa gaagcccgac aaatcggaag gtctcgatac taactggcgt
     3181 gacaagagtc ctgcagagcg ccaccgcgcc atgacctgga tgcaacgcct caagcgagtc
     3241 ttcaatattg atattgaagt ctgcgaacac tgcggcggtc acgtcaaagt gattgccagc
     3301 atcgaagatc cgaaggtcat tgagcagatt ctcaagcatc tgaaacagaa aacagccaag
     3361 gcgaatgccg ccaagcagcg tgagctgcca ccagaacgag cgccgccact gactcccagc
     3421 ctgttcgatc catcacagag tcgtctcttt gactgacgac cccaaatcca acactgctca
     3481 acactgccaa cttttaaacg gggcggtggg gcagtttgta tctctcgagc tatcaggcta
     3541 gagattttac cgccaaatcg aaccttatta gagcggttta ggctggaccg gcagttaaaa
     3601 ttggggcttg agcggtaaac gagtgaggga atttcaggta agatacttcg gatgaggagc
     3661 aaaaaggtgg tttatacttc ctatacccgc tcgcggtcga agccgcgtcc ggcctgcccg
     3721 aatacctgca atggctggcc gccgagagcc gaccggtaaa ctgagccaat gaccgcatcc
     3781 acgatccgcc tgcgccgctg gctggccggg ctcgccctgc tgcttgcgct gcccgcgacc
     3841 tcggccgtcg cccaggactt cgaactgccg ccggtcgacg aggtcttcgt cctgtccgcg
     3901 caggccaccg cgccggaccg catcgaggtg cgctggcgga tcgccgacgg ctattacctg
     3961 taccggcacc gcacctcggt caaggccgat gccgccttca ccggcgcgac catggcgctg
     4021 ccgaagggca aggcctaccg cgacgaattc ttcggcgacg tcgaaaccta ccgcaaggaa
     4081 ttgctcggca ccctcaccgg cacgcccgcg gccggcgcga gcgcgaccac cctgaccgtg
     4141 aagtaccagg gctgcgccga tgccggcgtg tgctacccgc cgcagacccg caccctgaag
     4201 gtcgcgttgc cgggcgaagc gggcgctggc ggcttcggct gtaaggcgcg cgggctgcat
     4261 gattatgtcg tcaagaacgg ctctgccgat catcccaacg ccgaggtgaa attcgcactg
     4321 ggtgatgtgg tcaacaccat gatcggctgc actaatggtg aaacgatcat gctgtgccac
     4381 gacacctcgc tgccgcgccc ctattctctc ggctttcggg tgcaaggcac cgaggggctg
     4441 tggatggacg tcaacaagtc gatctatctg gagggcaaga gcccacagcc gcaccgctgg
     4501 gagcctgccg agggctggtt tgcgaaatac gatcacccgc tatggaaacg ctacgccgat
     4561 ctggcggcag gggccgggca tggcgggatg gactggttcg tgatccacgc ttttgtcgag
     4621 gcgctgaagg ccaaggcccc gatgccaatc gacatttacg acgcgctggc ctggagcgcg
     4681 atcacgcctc tgtcggaaca atcgattgct gagggcaatc gcacgttaga ttttcccgac
     4741 ttcacccgag ggcagtggcg cacccgcaag ccgatctttg cgctgaacga cgcctattga
     4801 tcgacgcgat ttaggccaag cgcaccgcaa aggcgaaatt ggtaatcttg tcggtatcct
     4861 tgacgcccgg cgcgctttcg acgccgctgg aggtatcgac cagcggcgct ccggtgcgcg
     4921 caatcgcctc ggcaacattc gtcggattta gcccgcctgc cagcccccac ggcaaggcac
     4981 cgcgatatcc ggccagcagc gaccagtcga acgccaaccc catgccgccg ggcagcgcgc
     5041 ctttgggggt cttggcgtcg aacaagatca agtccgccgc cccggcatag gctgcggcgc
     5101 gtgcgacatc gctggcgctg gcgacgggca gcgccttcca caccggcttg ccaaaccgcg
     5161 cgcgcaactg ggccacgcgt tcgggcgatt ccgaaccgtg cagctgcagc gcgttcagct
     5221 tggctgccac cagtgcgtcg gcgatgacag catcatccgc atcgacgaac aaaccgacca
     5281 tggcgatctg gccagctgcg cgcgatgtca aagcgcccgc gacattcgac gtaaccgcac
     5341 ggggcgacgc tggatagaac accaacccgg catagtccgc ccgcgccgcg atggtcgcat
     5401 cgagcgcctc gggtgtgctg atcccgcaaa tcttgatttt cgcgggcatg cggtcagtcg
     5461 gggttctgga tcagccgcac cagcgtgcag tcgggatcga tcaggtagcc gatcctcagg
     5521 ccgctcgcct ccagttgcgg agctttgaag cgcggccagc cggtgctttt ttcctcggct
     5581 cccgccgcgt tcaccaatgc caccatggca tcgagatcat ccaaccgcag gcaacagccg
     5641 aacgagctcg tagctgggtc gaggtcagga taggggaaga attcgagctg caaaccgccg
     5701 cgctgcagga tcatccagcc gcgatccttc caactcgtcg caaagcccag cttcgcataa
     5761 aacgcctctg tcacatcgaa atcgcgcgat ggcagattgg gggtgacgtg gtcagccatg
     5821 gctcagcgca gcttgtcggc catgcgggcc gtatgagtga ttgcggcgcg gctatcgggg
     5881 gcggaatggc tcatcacgat catgctggcc ttggggaacg ccgcaccaaa cgcgcgcgct
     5941 gacgcggcgt agtgctcagt gtcggcatca ccgagattgc cgagcgactt ggccttgctg
     6001 tccttgatca ggcagccacc aaaagcgatg tcggtgccgt cgatcccaac ggtgatattg
     6061 tcactggtgt ggccggggcc ggggtaaaat accttgagcg ggccaaagtt gggcgcggtt
     6121 gctggttcga cccagccatt ggcggcgaaa gtcaggctgt gttgcgccgc aaccagcccc
     6181 tcttgcgggg caagctggtt cgacaacgca ttggcataag tcgcaatccc cgccgcatgc
     6241 agcgcgtcca taccgcccat cttgtcctga tgcgcgtgag tcaccaccgc cagcgcgacc
     6301 ggcaggttga tctcctgctt gatccagttg aggatctggg cggtctggtc atcggtccag
     6361 gcggtatcga ccaacagcac gcggccgcca tccctgacga tcaaaccgtt ggaagcgact
     6421 gccccgaaac ccggcatgtc gagataggaa gtgtgctgcc agacattcgg tgcgagctgg
     6481 cggaaaacca gatcgccaaa ccgttggtcg ccagtttcca tttgctggcc aatcgtcggg
     6541 cggatttcac cgggcatgca cccgctcagc atcaatgcag cggctaatgc ggtgctcagc
     6601 ttcgcgaccg ggtgcataat attgggcaat tccatcaagt tttcctttta ttcagcatta
     6661 aaaaccccgc aaatgcgagg cctagtaaat agatgatctt aatttggttc actgtagcaa
     6721 aaatatgggg cgaattcaaa catgaggtgc gacagtttca aaagccatat gataatcaac
     6781 aagctgagca aatttctcta atggtgtaag ccaatctaac gcctttctag gacgagtatt
     6841 cagtgacatg gcaacttgat ttaaataatg ctgatctgcc tgatttaaat caatcccttt
     6901 aggtaaatat tgcctaatta aaccattcat attttcgcat gtgccttttt gccagggtga
     6961 atgtgggtca cagaaatata catctatgcc taaatcttct tcgagtattt tatgttgagc
     7021 acttttcttt cacccaatgg gtgaaagctg aatttcgaag gaatgcatat ttatcaaggc
     7081 tttgattatg ctttttgaag tactgacgta gaatctaggt atgagtaaac ggcactgttg
     7141 caaatagtcg gtggtgataa acttatcatc cccttttgct gatggagctg cacatgaacc
     7201 cattcaaagg ccggcatttt cagcgtgaca tcattctgtg ggccgtacgc tggtactgca
     7261 aatacggcat cagttaccgt gagctgcagg agatgctggc tgaacgcgga gtgaatgtcg
     7321 atcactccac gatttaccgc tgggttcagc gttatgcgcc tgaaatggaa aaacggctgc
     7381 gctggtactg gcgtaaccct tccgatcttt gcccgtggca catggatgaa acctacgtga
     7441 aggtcaatgg ccgctgggcg tatctgtacc gggccgtcga cagccggggc cgcactgtcg
     7501 atttttatct ctcctcccgt cgtaacagca aagctgcata ccggtttctg ggtaaaatcc
     7561 tcaacaacgt gaagaagtgg cagatcccgc gattcatcaa cacggataaa gcgcccgcct
     7621 atggtcgcgc gcttgctctg ctcaaacgcg aaggccggtg cccgtctgac gttgaacacc
     7681 gacagattaa gtaccggaac aacgtgattg aatgcgatca tggcaaactg aaacggataa
     7741 tcggcgccac gctgggattt aaatccatga agacggctta cgccaccatc aaaggtattg
     7801 aggtgatgcg tgcactacgc aaaggccagg cctcagcatt ttattatggt gatcccctgg
     7861 gcgaaatgcg cctggtaagc agagtttttg aaatgtaagg cctttgaata agacaaaagg
     7921 ctgcctcatc gctaactttg caacagtgcc ctgcggagca aggccgtcgc gaacgagtgg
     7981 cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc acgtttgaag
     8041 gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct ccgtggatcg
     8101 gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga
     8161 tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc ctggtccttc
     8221 agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt
     8281 aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg
     8341 aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc cgttccatac
     8401 agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca
     8461 tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat ctcctgaagc
     8521 cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga
     8581 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
     8641 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     8701 cagtggacat aagcctgttc ggttggtaag ctgtaatgca agtagcgtat gcgctcacgc
     8761 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     8821 gcttgttatg actgtttttt tgtacagtct atgcctcggg catccaagca gcaagcgcgt
     8881 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     8941 cgccctaaaa caaagttagc catatgaact cggaatcagt acgcatttat ctcgttgctg
     9001 cgatgggagc caatcgggtt attggcaatg gtcctaatat cccctggaaa attccgggtg
     9061 agcagaagat ttttcgcaga ctcactgagg gaaaagtcgt tgtcatgggg cgaaagacct
     9121 ttgagtctat cggcaagcct ctaccgaacc gtcacacatt ggtaatctca cgccaagcta
     9181 actaccgcgc cactggctgc gtagttgttt caacgctgtc gcacgctatc gctttggcat
     9241 ccgaactcgg caatgaactc tacgtcgcgg gcggagctga gatatacact ctggcactac
     9301 ctcacgccca cggcgtgttt ctatctgagg tacatcaaac cttcgagggt gacgccttct
     9361 tcccaatgct caacgaaaca gaattcgagc ttgtctcaac cgaaaccatt caagctgtaa
     9421 ttccgtacac ccactccgtt tatgcgcgtc gaaacggcta accattccgt caacgggacg
     9481 ccaaaatgct gcgcattttg gttccctccg ctgcgctccg gctctcgtta cgtccaacgt
     9541 tagcaccact taaacccagc tttatttagc tcatgtttat tcaaacggca tttagctttt
     9601 caggcgttat tcagtgcctg ttttgccttt tttccgggct tcgcctgcat gggctgcgca
     9661 ggttttcagt ctttttggcc tctagccctt gcgtagcaag cgcaagcagc tatcgttttt
     9721 gcagtgctgt gccgcctcgg tggcgcagcg ttttttcacg gttagcgccc gtcgccaaat
     9781 tcaagttatc cgttttggct tctggttcta acatttcggt caagccgacc cgcattctgc
     9841 ggtcggctta cctcgcccgt tagacatcat gagggaagcg gtgaccatcg aaatttcgaa
     9901 ccaactatca gaggtgctaa gcgtcattga gcgccatctg gaatcaacgt tgctggccgt
     9961 gcatttgtac ggctccgcag tggatggcgg cctgaagcca tacagcgata ttgatttgtt
    10021 ggttactgtg gccgtaaagc ttgatgaaac gacgcggcga gcattgctca atgatcttat
    10081 ggaggcttcg gctttccctg gcgagagcga gacgctccgc gctatagaag tcacccttgt
    10141 cgtgcatgac gacatcatcc cgtggcgtta tccggctaag cgcgagctgc aatttggaga
    10201 atggcagcgc aatgacattc ttgcgggtat cttcgagcca gccatgatcg acattgatct
    10261 agctatcctg cttacaaaag caagagaaca tagcgttgcc ttggtaggtc cggcagcgga
    10321 ggaattcttt gacccggttc ctgaacagga tctattcgag gcgctgaggg aaaccttgaa
    10381 gctatggaac tcgcagcccg actgggccgg cgatgagcga aatgtagtgc ttacgttgtc
    10441 ccgcatttgg tacagcgcaa taaccggcaa aatcgcgccg aaggatgtcg ctgc
//
