LOCUS       CP157139                6733 bp    DNA     circular BCT 29-MAY-2024
DEFINITION  Escherichia coli strain UTAK-16 plasmid pUTAK-16-5-pColE3, complete
            sequence.
ACCESSION   CP157139
VERSION     CP157139.1
DBLINK      BioProject: PRJNA935790
            BioSample: SAMN41050887
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Insights into the Plasticity and Virulence of Escherichia coli
            Serogroup O45
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2024) South Texas Center For Emerging Infectious
            Disease, University of Texas at San Antonio, One UTSA Circle, San
            Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/23/2024 14:10:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,252
            CDSs (total)                      :: 6,121
            Genes (coding)                    :: 5,525
            CDSs (with protein)               :: 5,525
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 596
            CDSs (without protein)            :: 596
            Pseudo Genes (ambiguous residues) :: 0 of 596
            Pseudo Genes (frameshifted)       :: 405 of 596
            Pseudo Genes (incomplete)         :: 189 of 596
            Pseudo Genes (internal stop)      :: 59 of 596
            Pseudo Genes (multiple problems)  :: 50 of 596
            Pseudo Genes (short protein)      :: 1 of 596
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="UTAK-16"
                     /serotype="O45"
                     /isolation_source="culture"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pUTAK-16-5-pColE3"
                     /geo_loc_name="USA"
                     /collection_date="2013"
     gene            1..927
                     /locus_tag="ABH551_30870"
     CDS             1..927
                     /locus_tag="ABH551_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="XBF39137.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1145..1420)
                     /locus_tag="ABH551_30875"
     CDS             complement(1145..1420)
                     /locus_tag="ABH551_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="XBF39138.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
                     /name="mobilization protein"
     gene            1599..3798
                     /gene="mobQ"
                     /locus_tag="ABH551_30880"
                     /pseudo=""
     CDS             1599..3798
                     /gene="mobQ"
                     /locus_tag="ABH551_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     gene            4030..5775
                     /gene="col"
                     /locus_tag="ABH551_30885"
     CDS             4030..5775
                     /gene="col"
                     /locus_tag="ABH551_30885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="XBF39134.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            5778..6035
                     /locus_tag="ABH551_30890"
     CDS             5778..6035
                     /locus_tag="ABH551_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="XBF39135.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6097..6240
                     /locus_tag="ABH551_30895"
     CDS             6097..6240
                     /locus_tag="ABH551_30895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="XBF39136.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6733
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1425..1598)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(1599..1957)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..927
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.44"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2276..3795
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.83"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgtaaa aaatcccgtg
      241 aacggtcatg ctcacctgct ctatgcactc aacatcgccg tgagaaccgc gcctgatgcg
      301 tcggttaagg cactgaagta cgctgccgcg atagagcgtg cgctgtgtga gaaacttggt
      361 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg tatcgcttcc cgttccgctt
      661 tctccggcgg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgtggtcgca agggcgggaa aattggaggt gctaagtcta agcgtggtgc tgttgccaca
      841 tctgcgcgaa cgttgaaacc gtgggagact cttggaatta gccgcgcctg gtattaccaa
      901 ctgaaaaaac gaggtcttgt agagtagacc aaataaaacc tatatcagat aacagcgcct
      961 ttttggcgct tttttgagca gcttggtttg ttgctatttc cctcgttgaa tcccgcaatg
     1021 gcgcggcttt ccgcatgatt gaggttgtag cgctcgccgc agtctcatga ccgagcgtag
     1081 cgagcgaatg agcgaggaag cgcaaaggcg tccggtggtg catgtggctc ttacgcgccg
     1141 gggcttagtg gttctgcggt ttcgcaggtg gtttgggtag cttctccagc tcgttaatca
     1201 gcggttgtag tcggttcaca tccgcctgtc ttgtgacttc ctttcgcaga aactggagca
     1261 ggaacgcacg cagttgcgcc tcttccggcc tccgtaccct cgccagcatg gcagccccca
     1321 caatgacttt ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg
     1381 cctcagcttc ggcaatcttc tgttcgagtg ttctgctcat tttgtgactc cgtgcgcggt
     1441 gaaaaatcgc attttatcgc gtcgctggta gttgcaaaac tgaactggca taatgcacgg
     1501 cacatcacga agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg
     1561 acgctaaaag aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca
     1621 tgaaaatcat ttcgcgaaaa aacggctaca gtgctgttgc ttctgctgcc taccgttccg
     1681 gctctgtcat acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg
     1741 ttgatgatgc ggtcattctc acccctgcga atgcaccgtc ctggtgtggt gaccgttccg
     1801 ttctctggaa tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga
     1861 ttgaactcgc cattccccgt gagatttccc gcgaggccgc acgggagacc gttctcgctt
     1921 ttgtccggga aaactttgtc agtcggggca tgattgccga tgtggcgttc catcacatgg
     1981 accggaccaa tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacgggat
     2041 tcgcaggaaa ggtcagggac tggaacgacc gggcactcgc cgagacgtgg cgcgcatcat
     2101 gggctgacca tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc
     2161 gttcatacga gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctgcct
     2221 gtgcgatgga aaaacgggga atggaaacag aacgcggtga gcagaaccgc ctgattaaca
     2281 gcctcccgca caaggctggc agtcatccag gtacaggaga acgtgcggaa acgggaactc
     2341 agcgatgccg cacgtcgtgc agcggaagcc cttaacctga ccattcccgc tgcgaatgcc
     2401 tcagcggata ccctgcggga attcattgcc acgttgccgc aggaatgcgg caacgcgtgg
     2461 gagatgaccc cggagttcct ggcgatgagc gggaaggtga acgacatcga acgtgagggg
     2521 aatgcgctgc tgaaagagca ggccattctc gaaaaggaga tgaccggact caaaaaagca
     2581 cgccctgtcg cgtcccttct gtcagagatt cccctgatga catgggctga accggaatac
     2641 cgcaaaagac aactccgttt ctggaaactc gggaaacaga ttgaatctct tcgccgcacc
     2701 tacagggccg tgaaagaaca ggacattccc gcccgtcgtc aggcctttga aacgcagtgg
     2761 aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa aactgtcagc acgggaagcg
     2821 gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga aggaacagga gcatgaggcg
     2881 cggctgaaac gtcatgataa ccaccgtctg agccgtgaaa cggcattagt cggggttatt
     2941 acggagctgg ggcgtgccag agagccggga acgggcagga taacccgcta catgatgctg
     3001 agtaacagag ccggagaatt cacggtatgg ggtgatgagc tggcgcatta cccccagagt
     3061 gttcatgacc cggtgaatgt ttacctgtcg ctaggcgggg ctgtgatggt ctcggatata
     3121 cgtgagggaa tgccagaatc tcatgagacg atggcgcggc ctgagcgtgt gagaatgtat
     3181 tccggtgcga cggtccggca tgtactggaa cagatgcgcc aggggtggcc ctcttacggt
     3241 tttccggcgc tgccgcatca ctggccggat aatttttatt tcagcgacga ccgcaggccc
     3301 gtagcctctc cgctgccgtc agcgcaccgg gtggatatca ccgctgatgc tgtaccggaa
     3361 caactcatgc ctgttgtatt ttcgagagag ggaaacagca ggacgctgaa tctgttgtta
     3421 tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc gggaaggagg gttttgcccc
     3481 gttcttgtgc ttccgtcgcc ggattacagt catctgatag tcagcaccat cacggagaac
     3541 ggggtatgcc tggcaggtta cggagaagct ataaaccatg atgaggatac tccgtaccca
     3601 ccggaaccgc acctgatgca gttccggctc aaaggccatc atgacaggct tttggctgct
     3661 gtccacaaac cggaagagat gccggattat ctcttccgtc aactcggttt taatcagacc
     3721 tggcatgagt ggaagcggga cgaacagcac aggcaacaac aacgccgccc cgggcacttc
     3781 cggggcatga gtatgtgatg tccggggctg caccccggac cccgccaaca catcacgggc
     3841 cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt tatccctcct gatttctaaa
     3901 aattttccac ctgagcttga cagaaaaaac gatgacgggt actttttgat ctgtacataa
     3961 aaccagtggt tttatgtaca gtattaatca tgtaattaat tgttttaacg cttaaaagag
     4021 ggaattttta tgagcggtgg cgatggacgc ggccataaca cgggcgcgca tagcacaagt
     4081 ggtaacatta atggtggccc gaccgggctt ggtgtaggtg gtggtgcttc tgatggctcc
     4141 ggatggagtt cggaaaataa cccgtggggt ggtggttccg gtagcggcat tcactggggt
     4201 ggtggttccg gtcatggtaa tggcgggggg aatggtaatt ccggtggtgg ctcgggaaca
     4261 ggcggtaatc tgtcagcagt agctgtgcca gtggcatttg gttttccggc actttccact
     4321 ccaggagctg gcggtctggc ggtcagtatt tcagcgggag cattatcggc agctattgct
     4381 gatattatgg ctgccctgaa aggaccgttt aaatttggtc tttggggggt ggctttatat
     4441 ggtgtattgc catcacaaat agcgaaagat gaccccaata tgatgtcaaa gattgtgacg
     4501 tcattacccg cagatgatat tactgaatca cctgtcagtt cattacctct cgataaggca
     4561 acagtaaacg taaatgttcg tgttgttgat gatgtaaaag acgaacgaca gaatatttcg
     4621 gttgtttcag gtgttccgat gagtgttccg gtggttgatg caaaacctac cgaacgtccg
     4681 ggtgttttta cggcatcaat tccaggtgca cctgttctga atatttcagt taataacagt
     4741 acgccagcag tacagacatt aagcccaggt gttacaaata atactgataa ggatgttcgc
     4801 ccggcaggat ttactcaggg tggtaatacc agggatgcag ttattcgatt cccgaaggac
     4861 agcggtcata atgccgtata tgtttcagtg agtgatgttc ttagccctga ccaggtaaaa
     4921 caacgtcagg atgaagaaaa tcgccgtcag caggaatggg atgctacgca tccggttgaa
     4981 gcggctgagc gaaattatga acgcgcgcgt gcagagctga atcaggcaaa tgaagatgtt
     5041 gccagaaatc aggagcgaca ggctaaagct gttcaggttt ataattcgcg taaaagcgaa
     5101 cttgatgcag cgaataagac tcttgctgat gcaatagctg aaataaaaca atttgatcga
     5161 tttgcccatg acccaatgtc tggcggtcac agaatgtggc aaatggccgg gcttaaagcc
     5221 cagcgggcgc agacggatgt aaataataag caggctgcat ttgatgctgc tgcaaaagag
     5281 aagtcagatg ctgatgctgc attaagtgcc gcgcaggagc gccgcaaaca gaaggaaaat
     5341 aaagaaaagg acgctaagga taaattagat aaggagagta aacggaataa gccagggaag
     5401 gcgacaggta aaggtaaacc agttggtgat aaatggctgg atgatgcagg taaagattca
     5461 ggagcgccaa ttccagatcg cattgctgat aagttgcgtg ataaagaatt taaaaacttc
     5521 gacgattttc ggagaaaatt ctgggaagaa gtgtcgaaag atcctgagtt aagtaagcaa
     5581 tttaatccag gtaataaaaa acgcttgagc caaggattag ctccacgggc taggaataaa
     5641 gatactgtcg gtgggcgtcg ctcatttgag cttcatcacg acaagccaat cagtcaggat
     5701 ggtggtgttt atgatatgga caacctccgt atcactacgc cgaaacgcca tattgatatt
     5761 cacagaggtc agtaaaaatg gaactaaaaa acagcattag tgattacact gaaactgaat
     5821 tcaaaaaaat tattgaagac atcatcaatt gtgaaggtga tgaaaaaaaa caggatgata
     5881 acctcgagca ttttataagt gttactgagc atcctagtgg ttctgatctg atttattacc
     5941 cagaaggtaa taatgatggt agccctgaag ctgttattaa agagattaaa gaatggcgag
     6001 ctgctaacgg taagtcagga tttaaacagg gctgaaatat gaatgccagt tgtttaagga
     6061 tgaatgactg gcattctttc acaacaagga gtcgttatga aaaaaataac agggattatt
     6121 ttattgcttc ttgcagtcat tattctggct gcatgtcagg caaactatat ccgggatgtt
     6181 cagggcggga ccgtatctcc gtcatcaaca gctgaagtga ccggattagc aacgcagtaa
     6241 cccgaaatcc tctttgacaa aaacaaagcg tgtcaggctg attctgatgc gctttttttg
     6301 aaatgtcaca aaagttccat gtgggagatg ggatctaaaa tcctcgtgca gaactttcca
     6361 tccaggggga gaaaacttgt cgttttgagc cgttcggtgt tcagaacgca cgaaaccgat
     6421 cgcgcatcgc tttcgtgaat agttatgcag gcccctgaaa acgattctga cgcgtttttt
     6481 cggttttgcc tggtgttttc ctgtcttttt gcgttttttg cgtcagagcg cgtctgaggg
     6541 cgttttaagg ggtgcgtaca acgggagtta tggtaaatgg atcgggtttt cgggaaggtt
     6601 cgacaggatt tgccgttggg tgtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc
     6661 gtgtctctcc gccaagattg tcacgagaat acaggggcga ggtcggtcga taacctggaa
     6721 ggaatcgtaa cac
//
