LOCUS       CP157022                3924 bp    DNA     circular BCT 29-MAY-2024
DEFINITION  Staphylococcus aureus strain BSN35-3 plasmid pUSA01, complete
            sequence.
ACCESSION   CP157022
VERSION     CP157022.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637633
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN35-3 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN35-3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP062332, CP014442
            Genome Coverage                   :: 173.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/23/2024 08:00:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,923
            CDSs (total)                      :: 2,841
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 42 of 78
            Pseudo Genes (incomplete)         :: 34 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 16 of 78
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN35-3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pUSA01"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-09-26"
                     /collected_by="Andrew D. Berti"
     gene            1..147
                     /locus_tag="UXQ23_14595"
     CDS             1..147
                     /locus_tag="UXQ23_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="XBE59340.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVRFCCKVK
                     KYS"
                     /name="replication protein"
     gene            complement(175..849)
                     /locus_tag="UXQ23_14600"
     CDS             complement(175..849)
                     /locus_tag="UXQ23_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="XBE59341.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            865..1794
                     /locus_tag="UXQ23_14605"
     CDS             865..1794
                     /locus_tag="UXQ23_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="XBE59342.1"
                     /translation="MLKLYYKFNFATEPNVSYADILEILKIKKAFNVKQCGNILEFKPT
                     DEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVIEKVIKEKPKARWLFLTLSTKNA
                     IDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRSTEVTVNKNDGSYNQHMHVLLCVE
                     NAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAIKPNRKGDKDIESAIKETSKYSV
                     KSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLKQKHKILNLDDVEDGNLINASDE
                     DKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2378..2566
                     /locus_tag="UXQ23_14610"
     CDS             2378..2566
                     /locus_tag="UXQ23_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XBE59338.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2892..3473)
                     /locus_tag="UXQ23_14615"
     CDS             complement(2892..3473)
                     /locus_tag="UXQ23_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XBE59339.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1865..2029
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          907..1794
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.888"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gagaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaaggttc
      121 tgttgcaaag taaaaaaata tagctaacca ctaatttatc atgtcagtgt tcgcttaact
      181 tgctagcatg atgctaattt cgtggcatgg cgaaaatccg tagatctgaa gagacctgcg
      241 gttcttttta tatagagcgt aaatacactc aatacctttt aaagtattct ttgctgtatt
      301 gatactttga taccttgtct ttcttacttt aatatgacgg tgatcttgct caatgaggtt
      361 attcagatat ttcgatgtac aatggcagtc aggtttaagt ttaaaagctt taattacttt
      421 aaccattgct accttcgttg aaggtgcctg atctgtaatt accttttgag gtttaccaaa
      481 ttgtttaatg agacgtttga taaacgcata tgctgaatga ttatctcgtt gcttacgcaa
      541 ccaaatatct aatgtatgtc cctctgcatc aatggcacga tataaatagc tccattttcc
      601 ttttattttg atgtacgtct catcaatacg ccatttgtaa taagcttttt tatgcttttt
      661 cttccaaatt tgatataaaa ttggggcata ttcttgaacc caacggtaga ccgttgaatg
      721 atgaacgttt acaccacgtt cccttaatat ttcagatata tcacgataac tcaatgcata
      781 tcttagatag tagccaacag ctacagtgat aacatccttg ttaaattgtt tatatctgaa
      841 atagttcata cagaagactc ctttttgtta aaattatact ataaattcaa ctttgcaaca
      901 gaacctaatg taagctatgc agatatacta gaaattttaa aaatcaaaaa ggcttttaat
      961 gtaaaacaat gtggtaatat tttagaattt aagccaactg atgaaggcta tttgaagtta
     1021 cataagacat ggttttgtaa atcaaaatta tgtccggttt gtaattggag acgtgctatg
     1081 aaaaatagtt atcaagctca aaaagtgatt gaaaaagtaa ttaaggaaaa gccaaaagca
     1141 cgttggttgt ttttaacact ttcaacaaaa aatgcgatag atggagatac tttagaacaa
     1201 agtttgaagc atctaactaa agcatttgat aggttgagta gatataaaaa ggttaaacaa
     1261 aatcttgttg gatttatgcg ttcaacagaa gttaccgtta ataaaaatga cggtagttat
     1321 aatcagcaca tgcatgtttt gttatgtgtt gaaaatgcat attttagaaa aaaagagaat
     1381 tatataactc aagaagaatg ggttaattta tggcaaagag cattacaagt tgactatcga
     1441 cctgttgcta atgttaaagc gatcaaaccg aatagaaaag gcgataaaga cattgaatcg
     1501 gcaatcaaag agacctcaaa atattcggtt aaatcatctg attttttaac tgatgatgat
     1561 gaaaaaaatc aagaaattgt aagtgattta gaaaaaggtt tgtatcgaaa acgtatgtta
     1621 agttatggtg gattgcttaa acaaaaacat aaaattttaa acttagacga tgtcgaagat
     1681 ggtaatttga ttaatgcaag tgatgaagat aaaacaacag acgaagaaga aaaagcacat
     1741 tcaattaccg caatttggaa tttcgaaaag caaaattatt atttaagaca ttagtgttga
     1801 ctaatgtctt ttttgttgat tttttataaa aaagtactgt cttatttttg tgacaaatgc
     1861 tgtatgtagt gtcacaaaaa taagacaaac gcaatatatt gtgtcacaaa aataagacag
     1921 tacagctttg tatgatatca ctttaaaata ccttaaaacc cctggaattt ctggctttgc
     1981 cagacctatc atttttgaat gatagcaaat tctccttatg ctcttacgga gtttttagag
     2041 aaaaattaaa aattctcgat ttttgataaa aaacgccctg caggaattta gaaaaacatg
     2101 tggaagtttt aaaggatttt atgctaattt ttaatttgca tgtaaatggg cagtgttaaa
     2161 aaattagtca ctggttttgc tgaaattttt gtttgtgaaa ttaccatata tttatttggc
     2221 tcatatttgc gttttaagag cttgtatata tttttaagtt aaaaacctat atgatacccc
     2281 ctaaaccttt aaaatacccc cttaaaactc aaaataaagg tatttataat ttgagtaagt
     2341 cttgtgataa agttttttta aaaaaggagt gtttattttg aataaaaaca cagtgttaga
     2401 tgaaggttac gttatagcaa ctattttaaa tgttttcttt ttacttggat taatttttat
     2461 atctcatttg gaaaatttat atatattaat tccgtatgtt attttgatgg gaataaatgc
     2521 tatttatttg gttgttaagg ttatgaattt caaaaagaat aattagaata tttgagggga
     2581 ttttgaaacg agtttcttct tgttttcaca ctgttttttt attcctattg gtgttgttgc
     2641 ttactttttg tttttctata aagatgatgc ttttgacgag atggaagaaa aatatgatta
     2701 tcatgaagat aataaaaaat agacgacgca tttatgccga gaaaatttat tgatgttgag
     2761 aagaaccctt aactaaactt gcagacgaat gtcggcatag cgtgagctat taagccgacc
     2821 attcgacaag ttttgggatt gttaagggtt ccgaggctca acgtcaataa agcaattgga
     2881 ataaatagta tctaaagtaa cccgtattta agattacatt gtacatcctt cgaaaccata
     2941 gtagctgcga ttccagctag gtttcctttt atcccaattt tagctaattt ttttgctacg
     3001 ccctttaagt ttttggcttt aacatcttca aaaactgctg tgattatgtt tccagttaaa
     3061 agttctccat atgagtttaa aacttcagaa tagaaacagt caccgctatt tttgtagttc
     3121 ccaccaactg ctctaggtga tattgaacta gttttctttt tattctctat gtcgtttttt
     3181 agtttttgga atccttcagg aacatatcca tatcgagatt tggcattttc tagattaaca
     3241 tctttagctg ttccgtcttt atctaaaata ataattttgc catatatgta ttttagttct
     3301 ttttcaattt ctttttcgtt taaagtttca acgtttttat ctgcaatgtt atttttgttt
     3361 tgggcttgag cttctttgaa attaaaatcc aagaccattg aaagtatggt taaaatactt
     3421 aaagatacta ctaaaatctt tcttagtgat atactgtttt gaattgcatt cattgtaaat
     3481 gcccccttgt tttgttattt aaatgaattc aatataatat taacatataa aaacaaagga
     3541 gtttaatatg aataagtttt tttacttttt gattatttta ttatctgtat ttttaatttt
     3601 taattttgtt aattcgattt tatttgataa tccttttgac tttttagaaa gttttattta
     3661 ttcattggtt ttcacattaa tttatttagg tttagagggt cataaaaaga agaaggaata
     3721 ggttgttttt tgaaacgagt gtgaacgagt ttcttcttgt cttgatacta tatagaaata
     3781 actcgatttt atatatatag ctgtaactgt tgatattaca gtgtttaaac gtgtttttgt
     3841 gcgtgaaagg aaaatttgac aataaaaaac cccagttata ttattaaggt gtcgaatctt
     3901 aaataatact gggggtcttt ttat
//
