LOCUS       CP156947                4167 bp    DNA     circular BCT 28-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 2024CK-00456 plasmid unnamed4, complete
            sequence.
ACCESSION   CP156947
VERSION     CP156947.1
DBLINK      BioProject: PRJNA812595
            BioSample: SAMN41020286
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2024) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 4167)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/21/2024 13:48:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,027
            CDSs (total)                      :: 5,903
            Genes (coding)                    :: 5,744
            CDSs (with protein)               :: 5,744
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 57 of 159
            Pseudo Genes (incomplete)         :: 107 of 159
            Pseudo Genes (internal stop)      :: 24 of 159
            Pseudo Genes (multiple problems)  :: 25 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2024CK-00456"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2024-03-21"
     gene            complement(join(3104..4167,1..52))
                     /locus_tag="ABG872_00595"
     CDS             complement(join(3104..4167,1..52))
                     /locus_tag="ABG872_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005524 - ATP binding [Evidence IEA]; GO_function:
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="XBE17889.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            complement(397..510)
                     /locus_tag="ABG872_00600"
                     /pseudo=""
     CDS             complement(397..510)
                     /locus_tag="ABG872_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            926..1117
                     /locus_tag="ABG872_00605"
     CDS             926..1117
                     /locus_tag="ABG872_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="XBE17890.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1117..1518
                     /locus_tag="ABG872_00610"
     CDS             1117..1518
                     /locus_tag="ABG872_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="XBE17891.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1864..2106)
                     /locus_tag="ABG872_00615"
     CDS             complement(1864..2106)
                     /locus_tag="ABG872_00615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XBE17892.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2472..3104)
                     /locus_tag="ABG872_00620"
     CDS             complement(2472..3104)
                     /locus_tag="ABG872_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="XBE17888.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          complement(398..805)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gatatagctc attgaagcat ttgaagttct ttaaacttat ttcctttatc atgatagcct
       61 ctgttcgtgt ttttgttata tgccatacga tttgttagta ccttaataaa atgtacagct
      121 ttcattgtga ttagctagac tttatgtcaa agattaactt tgattgcttg tgttttaatc
      181 gatttttttt actttcttct tgagattatc tggcagcagc cgtaagcttc cggctgtgaa
      241 cgaaaaaaac cgccttggcg ggcggttttt tcgaaaggtt cagtagagtt ggcgctctat
      301 gaaccgaggt aactggctta aagggagcgc agtcaccaaa actgttcgtt tagtttagct
      361 ttaactggcg tataacttca agactaactc cctaaatcag ttaccagtgg ctgctgccag
      421 tggcgctttg tcgtgtcttt ccgggttgga ctcaagacga tagttaccgg attaggcgca
      481 gcagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
      541 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg
      601 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc atcaggggga aacgcctggt
      661 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt ccgtgatgct
      721 cgtcaggggg gcggagccta tggaaaaacg gccgctgtgc ggcctttcct tctttcctcc
      781 atgcgattac tcggttttct cgagcgtttc tacgcatgct cctgccgctc gccgcagccg
      841 aacggcagga gcgtagcgac tgagtgagcg aggaagcgga aaagaggaga tccctacact
      901 gaccactgac gctctgaggc ctcttatgac gaaacaacag aaaacagcgc tgaacatggc
      961 taaattcatc cagaatcagt ctctgctcct gctggagaag cttaacgagc ttgatctcga
     1021 tgtcgaggcc gacctttgtg agaaactcca cgatgatgcg gagcatcttt tccgtacact
     1081 gtcctcgaga ctggatgcat tacaggatgg taactgatgg ccagagtgtc tattagtgag
     1141 gctgcccgac tgacgggtaa gagtagaaca acactgcaca ggttaattaa agcaggtgac
     1201 ctgtccacct gtagcggtga gcgcaatgca aaaatggtag atacttcaga gcttttacgt
     1261 gtctttggcc cctttgagca accgaagggt gaacaccatt ctggacaggt ctctggacag
     1321 cgtgatacag cctctgctga ccagagtgaa caggtgatcc gtcatctgaa acaggagatt
     1381 gaacacctga acaccttact tttgtccaag gattcgcaca ttgacagcct gaaacaggcc
     1441 atgctccttc ttgaaaatca ccaggcaaaa ttaactgaat ccccggcgcc atggtggaaa
     1501 ttctggaaaa aatcctgacc ccgtcatgct tcagttttcc tctccggcca tcccagggag
     1561 ttcctgaacc ttcccaaaaa tcgtcagatc tctgaacgct cctcaccact aaaaataccc
     1621 acaccccacg caaaattaaa attttgcaga ttccttattt acaacaatgg gttaatgaaa
     1681 aataaatgtg cggtgatgtg ttacaaagtg cagatccaag tcgtggacga ctctatggcg
     1741 aatcgctgac gaattgccca cgattcatat gtgaatcacg ctttattatc atattgttat
     1801 gatgaatcga ctcttgattc aattcatcac tttgggtgtt ttttgctcat ggtgactctt
     1861 gattcaattc acgattcgaa tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc
     1921 ataacgtgat tgccggccgt cgacgaaccc gattcctgaa ggtgctgaaa accggttttc
     1981 cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc
     2041 ggaggagaag aacgccggaa acaggacttc ccctggctga atggtgtgaa tttccgcgct
     2101 atgcacttgc gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg
     2161 ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgtcc tgagtccgtt
     2221 ttagcggtgc gtacaattat gattttatgt taaattgttt taagtgaata gagagaatat
     2281 aacttaaggg agcatcttgt gctgggtctt ttacattaga tctgcacaat cattgttttg
     2341 gcatctggtg atgtctttag gacgtttgag attgctgata actaaagtgt atgatgcttt
     2401 ctatacgtta tccggcgttt aataacaccc tggtgataag ccagcccgtg aagggctggc
     2461 ttgatttgga tttatatttt tgaaataaaa tcttttattt catcaaagca attgtctgct
     2521 gtgtttatta ttccattttt catggatatt cctattcttg agtcggattc tctattgcag
     2581 ctaagataga cttgtacctt agcttttttt tcgtttgttg gataagtgta ctctttgtca
     2641 ctcgctgaga tgtcatcttt attattttta attttttcga aaaattctat tatgtattca
     2701 tataacgctc gagtgcttgc gtgttcaatt atcagatctt caatggcacc ttgcttgtct
     2761 tttcctggca ttataaagaa agaaattttt gtattgccat tcatagctat ctcgttatgc
     2821 gagctggggg aggagatttg tagttctcgc aatgtggcaa ttattgatct ttctgtgtca
     2881 gaaaatgatg tgtcagcatc taatataata aaaatacttt ctttttcatc gaaagaatca
     2941 atgtttttga atgctctaag tgcttgtttt aattttgatt ttccatttat tttttcgaca
     3001 aaaacatcgg taatgccttg tatttcaagt aggttgcata gaaaatgaaa atcatcatca
     3061 ccctcaacga aaatgatctt tttgctatta tttatgtctt tcattatctt acctcccagt
     3121 cgttctcaac tgagtaggag aaatcatcca tattgaattg tacggctgtt ggttttaggg
     3181 agttcttttc tcttcctagt ctaatataag cgatatcttc atttttgcta tctatttcgt
     3241 ttattgctcg tatcacatct tgactatgag ttgttgcaaa tatttgattg ttgttttgtt
     3301 ttgcagatga aattaatgat tttattatgt ctttaattac tgagtaatgt attccgttct
     3361 cgatttcatc aataagaata attgagtttt cctgtaccag cactgagctg ataaaagata
     3421 atgctcgact gataccctcg ccaagcattg atatttcatt tagctcacta aatccaatgt
     3481 ctaaatatat ttccttattg ttacctatcg cagatattgc tatatctaca attctgtcat
     3541 ctattttttt taagttttct agtatgtctt ttttcctttt ttgtgttagt aggctagata
     3601 catttgtaat tgttgcctct ttgtttatct ttctggatgt tgttattatt gttgctgtct
     3661 taaatttata attaatccca tccctttcgt tttttatttc ggattttatt tctacgccat
     3721 cttctttcaa ttgcatagag aaaagcttat tggaaaccct gtttttcgca ttgggtgtct
     3781 cggtcacatt tatttttaaa gagtttactg tggcctgaga accgatagtt tgacgaggta
     3841 tgttttggtt agatagtacg gtgtcaacgt ttagagacaa acttgattct aatctctgat
     3901 ttgtttctgt ttcatatgtt tgtgttactt cagagcgttc atctcgaatg actatggaca
     3961 tcttttgtga tgtgtccata tcatgaaagt aactaaccca aaatggcttg tttttgttta
     4021 tttctgccat ttcttttctg aatgcttgaa tgtttaacaa aacagccggg ttttttacat
     4081 cataaaaaca aaaaatagca tccagtatag atgttttccc gtagttgttt ttcccagcta
     4141 tgatatttaa agtctttaat tgcttta
//
