LOCUS       CP156702                2473 bp    DNA     circular BCT 26-MAY-2024
DEFINITION  Staphylococcus aureus strain BSN144 plasmid pCN1, complete sequence.
ACCESSION   CP156702
VERSION     CP156702.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637723
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN144 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN144
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP043386, CP014445, CP014442, CP003981
            Genome Coverage                   :: 144.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/20/2024 08:52:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,918
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 45 of 88
            Pseudo Genes (internal stop)      :: 17 of 88
            Pseudo Genes (multiple problems)  :: 20 of 88
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN144"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pCN1"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-10-22"
                     /collected_by="Andrew D. Berti"
     gene            84..818
                     /gene="erm(C)"
                     /locus_tag="UXR69_14815"
     ARG             85..815
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1266..1742
                     /locus_tag="UXR69_14820"
     CDS             1266..1742
                     /locus_tag="UXR69_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="XBD14384.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             85..818
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.18"
                     /coverage="100.0"
     Typing          1267..1752
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatcaacc aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatta
       61 taaccaaatt aaagagggtt ataatgaacg agaaaaatat aaaacacagt caaaacttta
      121 ttacttcaaa acataatata gataaaataa tgacaaatat aagattaaat gaacatgata
      181 atatctttga aatcggctca ggaaaaggcc attttaccct tgaattagta cagaggtgta
      241 atttcgtaac tgccattgaa atagaccata aattatgcaa aactacagaa aataaacttg
      301 ttgatcacga taatttccaa gttttaaaca aggatatatt gcagtttaaa tttcctaaaa
      361 accaatccta taaaatattt ggtaatatac cttataacat aagtacagat ataatacgca
      421 aaattgtttt tgatagtata gctgatgaga tttatttaat cgtggaatac gggtttgcta
      481 aaagattatt aaatacaaaa cgctcattgg cattactttt aatggcagaa gttgatattt
      541 ctatattaag tatggttcca agagaatatt ttcatcctaa acctaaagtg aatagctcac
      601 ttatcagatt aaatagaaaa aaatcaagaa tatcacacaa agataaacag aagtataatt
      661 atttcgttat gaaatgggtt aacaaagaat acaagaaaat atttacaaaa aatcaattta
      721 acaattcctt aaaacatgca ggaattgacg atttaaacaa tattagcttt gaacaattct
      781 tatctctttt caatagctat aaattattta ataagtaagt taagggatgc ataaactgca
      841 tcccttaact tgtttttcgt gtacctattt tttgtgaatc gattatgtct tttgcgcatt
      901 cgcttctttt ctatataaat atgagcgaag cgaataagcg tcggaaaagc agcaaaaagt
      961 ttcctttttg ctgttggagc atgggggttc agggggtgca gtatctgacg tcaatgccga
     1021 gcgaaagcga gccgaagggt agcatttacg ttagataacc ccctgatatg ctccgacgct
     1081 ttatatagaa aagaagattc aactaggtaa aatcttaata taggttgaga tgataaggtt
     1141 tataaggaat ttgtttgttc taatttttca ctcattttgt tctaatttct tttaacaaat
     1201 gttctttttt ttttagaaca gttatgatat agttagaata gtttaaaata aggagtgaga
     1261 aaaagatgaa agaaagatat ggaacagtct ataaaggctc tcagaggctc atagacgaag
     1321 aaagtggaga agtcatagag gtagacaagt tataccgtaa acaaacgtct ggtaacttcg
     1381 taaaggcata tatagtgcaa ttaataagta tgttagatat gattggcgga aaaaaactta
     1441 aaatcgttaa ctatatccta gataatgtcc acttaagtaa caatacaatg atagctacaa
     1501 caagagaaat agcaaaagct acaggaacaa gtctacaaac agtaataaca acacttaaaa
     1561 tcttagaaga aggaaatatt ataaaaagaa aaactggagt attaatgtta aaccctgaac
     1621 tactaatgag aggcgacgac caaaaacaaa aatacctctt actcgaattt gggaactttg
     1681 agcaagaggc aaatgaaaaa caagaaaatg cattatctga ttattattct ttcaaggact
     1741 agtataacat aaaatcgtct acaaatagac aaaaaacctg cacgcttaat gtagatcaaa
     1801 agcttaacgc aaatgaaata gattgacctc ccaataacac cacgtagtta ttgggaggtc
     1861 aatctatgaa atgcgattaa gctttttcta attcgcataa gcgtgcaggt ttaaagtaca
     1921 taaaaaatat aatgaaaaaa agcatcatta tactaacgtt ataccaacat tatactctca
     1981 ttatactaat tgcttattcc aatttcctat tggttggaac caacaggcgt tagtgtgttg
     2041 ttgagttggt actttcatgg gattaatccc atgaaacccc caaccaactc gccaaagctt
     2101 tggctaacac acacgccatt ccaaccaata gttttctcgg cataaagcca tgctctgacg
     2161 cttaagtgca ctaatgcctt taaaaaacat taaagtctaa cacactagac ttatttactt
     2221 cgtaattaag tcgttaaacc gtgtgctcta cgaccaaaac tataaaacct ttaagaactt
     2281 tcttttttct tgtaaaaaaa gaaactagat aaatctctca tatcttttat tcaataatcg
     2341 catcagattg cagtataaat ttaacgatca ctcatcatgt tcatatttat cagagctcgt
     2401 gctataatta tactaatttt ataaggagga aaaaatatgg acatttttag tatttttgta
     2461 atcagcacag tta
//
