LOCUS       CP155650               10077 bp    DNA     circular BCT 20-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SC-KP208 plasmid pSC-KP208-3, complete
            sequence.
ACCESSION   CP155650
VERSION     CP155650.1
DBLINK      BioProject: PRJNA1110013
            BioSample: SAMN41315612
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Kuo,P.Y.
  TITLE     urinary tract infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Kuo,P.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2024) College of Life Sciences, National Yang Ming
            Chiao Tung University, Linong Street, Taipei 112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 15.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/14/2024 14:53:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,108
            CDSs (total)                      :: 4,999
            Genes (coding)                    :: 4,865
            CDSs (with protein)               :: 4,865
            Genes (RNA)                       :: 109
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 54 of 134
            Pseudo Genes (incomplete)         :: 82 of 134
            Pseudo Genes (internal stop)      :: 17 of 134
            Pseudo Genes (multiple problems)  :: 17 of 134
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC-KP208"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pSC-KP208-3"
                     /geo_loc_name="Taiwan"
                     /lat_lon="23.00254450 N 120.21938242 E"
                     /collection_date="2020-07-21"
                     /collected_by="NCKUH"
     gene            join(9823..10077,1..276)
                     /locus_tag="ABDV15_00005"
     CDS             join(9823..10077,1..276)
                     /locus_tag="ABDV15_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XAX43416.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(304..483)
                     /locus_tag="ABDV15_00010"
     CDS             complement(304..483)
                     /locus_tag="ABDV15_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="XAX43417.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(509..937)
                     /locus_tag="ABDV15_00015"
     CDS             complement(509..937)
                     /locus_tag="ABDV15_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XAX43418.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1101..1415)
                     /locus_tag="ABDV15_00020"
     CDS             complement(1101..1415)
                     /locus_tag="ABDV15_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="XAX43419.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1403..1681)
                     /locus_tag="ABDV15_00025"
     CDS             complement(1403..1681)
                     /locus_tag="ABDV15_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="XAX43420.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2023..2241)
                     /locus_tag="ABDV15_00030"
     CDS             complement(2023..2241)
                     /locus_tag="ABDV15_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="XAX43421.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            complement(2238..2609)
                     /gene="tolA"
                     /locus_tag="ABDV15_00035"
     CDS             complement(2238..2609)
                     /gene="tolA"
                     /locus_tag="ABDV15_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="XAX43422.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2883..3128)
                     /locus_tag="ABDV15_00040"
     CDS             complement(2883..3128)
                     /locus_tag="ABDV15_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XAX43423.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3455..5140
                     /locus_tag="ABDV15_00045"
     CDS             3455..5140
                     /locus_tag="ABDV15_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="XAX43424.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5150..5407
                     /locus_tag="ABDV15_00050"
     CDS             5150..5407
                     /locus_tag="ABDV15_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="XAX43412.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5492..5641
                     /locus_tag="ABDV15_00055"
     CDS             5492..5641
                     /locus_tag="ABDV15_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="XAX43413.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7124..9085
                     /locus_tag="ABDV15_00060"
     CDS             7124..9085
                     /locus_tag="ABDV15_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="XAX43414.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            9085..9816
                     /gene="mobC"
                     /locus_tag="ABDV15_00065"
     CDS             9085..9816
                     /gene="mobC"
                     /locus_tag="ABDV15_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="XAX43415.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10077
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5745..6323)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.938"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9086..9813
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.74e-141"
ORIGIN
        1 ccggccggaa tgaagctgtg tgattatctt tatcgcaagg ttgagcagtt cgccatcgag
       61 aacggcgtgc aggtactgga cgccgggaaa tacaccccgg agcagatcgg ggcgatgatt
      121 gacgtcgtga gtgagcggta cggggaaagc gcggtgaacg agaagctgaa agcggagctg
      181 atgcgggaat ataacagccg ctattttaac gttccggtgc cagaactgcc ggaggataaa
      241 cccgcccgcc ggaggtggcc gttcggcaaa aaataaacgc cagcaccggc catgccggtg
      301 ctgttattct tccccgagct tatcctgcat cacctgccag agtgtttccg catcctcgtg
      361 cagccgctcg cacatgtcgg ccggggtatc catatccagc tcgttcagtt tctcgagcaa
      421 cagcagcgac tggtccttaa tgaatttagc catattgagg gcggtttttt cctgcttcgt
      481 catgttctcc attcccttcg gtaagttctc agctaaacca gccggaccag aagcgttttt
      541 tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc ctggagtttc cggcgctcat
      601 catcgtgctg gcggcgttct tcttctcggg atgttttatc ttccagcatc agggtgacag
      661 cctgccggag atcggccagt tccttttgca ttgccagaag ctgttcctgt gggaaatttt
      721 tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt ctcagggata ttcacatttc
      781 cgtatacacg gatcagttct gacacatcca cgacagggtt gtttttcccg tcacgtgaca
      841 cggtaacctt tccctgtttt acatgattgt atagcgttct tctggtaatg cccgcggctt
      901 gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt atacccctga ttttatcggg
      961 tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc gtttttccgt
     1021 tcaggatgtg gttttctgac gcgctgtgct tgtcaaaccg gcgacggcca cgcaatgaat
     1081 tgcgcccccg tcatcggtta ttagaggctg tctgcccccg gatacccgga ttctttctga
     1141 tgcctgacgg tgacgatcac aatccggtcc atttcccggt cgtggcggta gagcatcaca
     1201 tagccactgt cgccaaaccc gatcaccagt tcctgatatt ccagcggcag aaatggcacc
     1261 gggcggccaa tgtctggcaa tgttttcagt tgctggatag cccggacgat cacctcaccg
     1321 gcttttctgg ctgccagccg atttttggtt ttgagaaagt cctggaggcg ttgtaaatct
     1381 cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc tcgttctcag
     1441 ttccccaggt acttatccag gcttctgctt cttccgcagt aaggtgcaga cccgtttcct
     1501 gatagtgctg ccatgcggct tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt
     1561 cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg
     1621 ccagcgtttt cagccggtcc ttaagttctt catcgagcct gatggatgtt gctgatgaca
     1681 taaaagcccc tttgtagtca tgtgtaatac atatgactac tttaatccgt ggcggcgcta
     1741 tttgccagaa atacggcttt tgctacccat ttttcagctt cgcttcccca tttttcagct
     1801 tcgcttccac taatcagggc tgctacaggc tcactcatca tccaggacta ttgcagcctg
     1861 cgggttaaag gacctgttac tggcggcaac catcatcatt gaactttttg gtggtggctg
     1921 gtcgcggatc ctgtaagccg tcatttttca gactgaattc aaaatttata acctgtcgca
     1981 ttgccgggtg ccctgcggga tatagccagt gcctcattgc cgtcagcgtt tcccgaaaaa
     2041 aaagtgacag ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt
     2101 ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag
     2161 tgtggttacg ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac
     2221 tgtcagagta aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag
     2281 gtattttcat ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta
     2341 cagagtttcg gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg
     2401 gatatccgca cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca
     2461 acagcggtct tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa
     2521 attttcggcg gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat
     2581 gccagcagga taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatatta
     2641 catgtatatt tgccactctt tacggccacg ttcattgaca taaacacggc ttatcgtatc
     2701 gtttttacac caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat
     2761 ttaccgggcg cgtttccggc ggttttccgg gtgatttgtt gcctgtttta tcccgtagcc
     2821 gccggaaacg ctctgagggc ttctcgggcg gtgctgataa cgggttttat ggtaaatacc
     2881 cctcaccggc ggcgcagcgg ctgccaggtg aaggtggttt ccgccgcctc gtatttctcc
     2941 cgacgcattt cctgcgcccg cctggcttca cgttcgtcct gaatggcgcg aagcaccctt
     3001 tcccgcacct gctgttttac cgcctttggc agcgttctgt tccctttcag gcccatggcc
     3061 agtgcctgag cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg
     3121 tgtgtcatgg tggcctccgg gcgtctcagg tttcctgaac agtttaaccg cgggtcctcc
     3181 ccccatacca gttagctttc agatgtgtct ataccacctg aaatgtaagt gtgatgtaca
     3241 tctcaaataa taaaatttgt tgccgtgaga aattttcggg aaaagacgcg atctgcttac
     3301 aggtggctcc gggcgctttt ttgtgcctga agatgagaac taatctcata tattgacacc
     3361 tgaaaacggg aggagtaagg taataattat actgtgtata tgtacagtat gttggcttta
     3421 atttattgtt ttaaatgtca aagaggaaaa cgatatgagt ggtggagacg gtcgaggtcc
     3481 gggtaattca ggtctgggac ataatggtgg tcaggccagt gggaatgtga acggtacgtc
     3541 tggtaaaggt ggcccttcat caggtggtgg tacggatcca aacagcgggc cgggctgggg
     3601 tacgacgcat acgccgaatg gagatattca taactataat ccgggggagt ttggtaacgg
     3661 cgggagtaaa cccggtggta atggtggtaa cagcggcaat catagcggta gctctggtgg
     3721 cggacagtct tcggccactg cgatggcctt cggtctgcct gccctggcta ctccgggggc
     3781 tgaaggactg gctttatcag tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct
     3841 ggctgccctg aaggggccgt ttaagtttgg tctgtggggg attgcgatct acggtgtgct
     3901 gccttctgag attgcaaaag atgatccgaa aatgatgtca aaaattatga cgtcattacc
     3961 ggccgatgcg gtgacggaga ctccggcaag tactttacca ctggaccagg cgacggttcg
     4021 tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg cggttgtcgc
     4081 aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt
     4141 cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac
     4201 agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc cggctggttt
     4261 tacagccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa
     4321 gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaaac agcgtcagga
     4381 ggaagaaaag cgtcgccagc aggcatggga cgccgctcat ccggaagagg ggctgaaaag
     4441 agactatgat aaagcgaaag ctgagctgga tgccgaagat aaaaatattg cgaccttaaa
     4501 cagccgcatt gcatcgacag agaaggcgct ccccggtgca agggctgctg ttcaggaagc
     4561 cgataaaaag gtaaaagagg cagaggcaaa taaagatgat tttgtgactt ataacccgcc
     4621 tcatgaatat ggctccgggt ggcaggatca ggttcgctat cttgataagg atattcagaa
     4681 tcagaatgag aaattaaaag cggctcaggc atctttaaac gcaatgaatg aatccttatc
     4741 cagagataag gctgcactta ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc
     4801 gaaggatgca gaaaataagt taaatgagga aaagaacaag cctcgcaagg ggactaaaga
     4861 ctacggccat gattatcatc cagtacctaa aacagaagac ataaagggac ttggagagtt
     4921 aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg cccgatggta
     4981 tggcgataaa aaacgtaaaa tttatgaatg ggattcccag cacggtgagc ttgaagggta
     5041 tcgtgccagt gatggcgaac acctcggggc atttgatccc aaaacgggta agcaggttaa
     5101 agggccagat ccaaaacgga acattaaaaa atatctttga gaggtaaata tggggcttaa
     5161 attacgtttg acatggtttg ataagaaaac tgaagagttt aaaggggaag agtattctaa
     5221 agactttggt gacgatggtt ctgtcattga aagcttggga atgcctttaa aggataatat
     5281 taataatggt gagtttgatt tgaagaaaga atgggttcct ttactacagc cattttttaa
     5341 aaatcaaata aaacctgatg tggataattg ttatatagct tttgattatc gggacggtaa
     5401 ctggtaacaa aaaattatca aggatgattt gaaatactga cttccggtgt tcagggatga
     5461 atgccggagt ctttaaatac agggaaagta tatgaaaaag gtaaagacta tttttctttt
     5521 tatattgatt gttgctggct ttcttctggc cgcatgtcag gcaaattata tccgggatgt
     5581 tcagggcgga acggtggcac catcgtcctc ctctgaactg acggggatcg cggttcagta
     5641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctt ttgccctgta
     5701 aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt aagtcagttg gggaactgct
     5761 taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc
     5821 ggactttggc gcacacttca agagcaacct cgtgtttagc taaacaaatc ctctgcgaac
     5881 tcccagttac caatggctgc tgccagtggc gttttgcgtg ctttcccggg ttggactcaa
     5941 gtgaacagtt accggataag gcgcagcagt cgggctgaac ggggagttct tgcttacagc
     6001 ccagcttgga gcgaacgacc tacaccgggc cgagatacca gtgtgtgagc tatgagaaag
     6061 cgccacactt cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc agggtcggaa
     6121 caggagagcg caagagggag cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg
     6181 gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa
     6241 aagcgtctgt ttatgtgaac tctggcagga gggcggagcc tatggaaaaa cgccaccggc
     6301 gcggccctgc cgttttgcct cacatgctcg ttccctgctt atccacggaa tctgtgggta
     6361 actttgcgag agttcgcagc gcccgccgca gtctcacggc cggcgcgtag cgaccgagtg
     6421 agcgaggagg cgcaatttcc ccggcacctg caagacggtc accggacgcg tggatgtgac
     6481 atattgctcc gtgttaccgc ctttctcttc cctgccgata aattattcag ataggtgaag
     6541 tctgtcggtt ttctgctgaa tattttacgt taacctatct ctgttgcttt tttctccttg
     6601 cggagaaatg cacgtcatcc ccgctggggc gtgactgact tcagggggaa atcccctgct
     6661 tacgctggct ggacgccacc gacgcactgt ctttccccct ctttctggct ctgtccggct
     6721 ggacgccggt cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac
     6781 aaaatccacg gtgctggacg cacctgacga agcagcaaag ctgctcctga ttttgcgggt
     6841 ggtcagccgc tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact
     6901 ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc
     6961 cttttccgca tggggcggaa agaggctggc ctccgcggtg aaagctgaac gcaatcacca
     7021 cggacgtgac cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcaggggctg
     7081 cacaacacgc cggtaaaccc gtcgtctgaa ggagagagat tgcatgttta atacggattc
     7141 gcttgcctgg cagtggcata tcccggagct ggcccggcag atttacccgt gggtcagctg
     7201 gctgtaccgg gagtattaca gtctggccgg tttttttctg gttatcggcc tgtttttcgg
     7261 tctggcagaa ctctgggccg ggcggatgcg attcgtgggg ctgagtcata aggagcggcg
     7321 ggagtatgcc gatcgctata agcgcgataa taaccggact gcgccatttt cggtgctggc
     7381 ctcgctggat tcgcccgggc ggatggtcag ggcacggcga catgtctgga actacgcata
     7441 ctggctgacc ggcgctgccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat
     7501 cgagagctgg ggagaaaccg tcctgcgtcc ggacctctac gcgaacgcag attattacct
     7561 gctgcagccg gctaacctgt tcgggattgc ctggtgtgcc ggaggtcttg tcgcaggcgt
     7621 cctcctggcg cggctgctgg cgttcctgct gcttgacggg cattttgcgg ccgctgacga
     7681 ggcggtgaac gcgaaactga atcaggagag ccggcgcagc agccagcgga ccggagaaat
     7741 gaccgacgtc cgccacctgc atttcgggga gccggtaccg gttaacgcgc tggccgattt
     7801 ttccacggag caggcccgga aacagcaggc ggtgtttctg ggtaaggatg agcagggcca
     7861 gccggtgctg gtcccgcgtg atacctggcg caagaccaac attcagatcc tcggcctgcc
     7921 cgggagcggc aagagcgtga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg
     7981 ggacgcggtg gtgtacttcg acccgaaagg ggacgcgtgg gcgccgcacg tcttccgggc
     8041 gcactgcccg gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct
     8101 gttccgcgat ctcgaccagt acgccctgaa gaatcttctg gttgccggat ttaacctgag
     8161 tgagaccggc gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat
     8221 tgcggagcag tttccgcagg gggcaaatat tcagcaggtg ctggcggcgg cgtacgcact
     8281 gccggaggcc ctgaaaaagg acgtgaaggg gctgatcacc aagctggaga acgtggccga
     8341 cctgagcgtc cttcagacgg acagcggcat cgacgtggcg gggataatca acggcggcgg
     8401 ctgcctgtac gtcatcggct cgatggacga tgaggcggtg atccgggtgc agaagatgct
     8461 gtttgcccgc tgcgcgcaaa tcatcattgc gcgggacgag ttccggaggt ggccgcacgc
     8521 gagcatcatg ctcgatgaga ttaaatatct gctgtcgaag tacgtgctga acgcgctggg
     8581 gacgctgcgc agccgggact gcaacctgct gctcgcgcac cagtcgctgg gcgacttcgg
     8641 ccagtgcggg caggatctgc ccgctgattt tgtgaaaacc acggtactgg ataacacgcc
     8701 gatccgctgg ttctaccggg cggccagcca ggagtccgct cagtgggcgg ccggacagac
     8761 cggggagatc cgggtggacg tggagcgccg ccgggccagc cgggaggcgg ggaacgtgga
     8821 gcatatcagc ggggacagct tcatccagaa ggaggcgcgg ccgctgtttg acgtgaacac
     8881 cctgcaacat ctgcctgacg ggttcgcggt gatgaccggg ctgggcgtgg cgcggctggc
     8941 gttcagcagc ccgctgcgtg ttgatcgccg ggaaattccg ctgaaaacct tccctgtgct
     9001 ggcgaaaacg gacccgctgg cggagtacca gtcggaggcc gggcggcagc ggccgggtga
     9061 tgatgatttt gcggggctgt actgatggca ctggagcgat acaacgtcag ccacgcgaag
     9121 cggcaggcac ggaacgcgga gaaaacacgg ctgaccctgc gctggctgcg ggaagaactg
     9181 tgttccacgg cggagctggt tgcgcaacgg ctggggattg cggcggtaca gccggtttac
     9241 cgcttcctgg acagcctggt ggcgaaaggg ctgctggtcc gggcgaaata cccggttgac
     9301 ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg tggcgttctc gttcgacgag
     9361 gacgagccgc tgacggacat tattccgttt cagccgtcgc gggtgtcggc ggcgcagctg
     9421 ccgcaccggc tggccgtgca gtccctgcgc ctcgccatgg aggcccgggg ggccaccggc
     9481 tggcggtacc tgcaccgtat tgccctgaaa gggatgaagg tgccggacgc gctggcggag
     9541 ctggacggca gaacggtggc gtttgaggtg gaacgtaccg tcaagagccg tcgccggtat
     9601 caggaagtgg tgtccgggta tctgttcaac cgtaaggcga acggcatcga tgaaatctgg
     9661 tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc gggcgattct ggcggtggat
     9721 gaaatcgtga acccgcagac cggcgaggcc cgcaaaacgg cggagctgga ccgggagagg
     9781 ctgttcgcat gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta
     9841 tttattgagg acgtggaaga caaagacggc gagcgctgca ttatgccaac cagcgagcat
     9901 ctggaagaca tggcggtcat ggcctggcag accggtgcat atgtattcag gtttgaagat
     9961 caggcccagc atctggcaat gatgaagttt atcggcatgt atcacgaacg gctgaacagc
    10021 gatgacaaag ccacgtatta cttcaccggc aaaaatccgg cgtgggaacg tgagaaa
//
