LOCUS       CP154384                2462 bp    DNA     circular BCT 06-MAY-2024
DEFINITION  Staphylococcus aureus strain BSN200 plasmid pSR42, complete
            sequence.
ACCESSION   CP154384
VERSION     CP154384.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637780
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN200 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN200
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: AP017320, MN216340
            Genome Coverage                   :: 156.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/30/2024 10:27:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,801
            Genes (coding)                    :: 2,725
            CDSs (with protein)               :: 2,725
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 42 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 28 of 76
            Pseudo Genes (multiple problems)  :: 27 of 76
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN200"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSR42"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2018-03-14"
                     /collected_by="Andrew D. Berti"
     gene            complement(join(2163..2462,1..177))
                     /locus_tag="UXQ53_14390"
     CDS             complement(join(2163..2462,1..177))
                     /locus_tag="UXQ53_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="XAL45030.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(625..1359)
                     /gene="erm(C)"
                     /locus_tag="UXQ53_14395"
     ARG             complement(629..1359)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1420..1479)
                     /gene="ermCL"
                     /locus_tag="UXQ53_14400"
     CDS             complement(1420..1479)
                     /gene="ermCL"
                     /locus_tag="UXQ53_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="XAL45032.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(626..1359)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(2..177)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.06e-87"
     Typing          complement(2164..2459)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.564"
                     /coverage="99.0"
                     /evalue="6.14e-77"
ORIGIN
        1 tttaagtttt tttccgccaa tcatatctaa catacttatt aattgcacta tatatgcctt
       61 tacgaagtta ccagacgttt gtttacggta taacttgtct acctctatga cttctccact
      121 ttcttcgtct atgagcctct gagagccttt atagactgtt ccatatcttt ctttcatctt
      181 tttctcactc cttattttaa actattctaa ctatatcata actgttctaa aaaaaaaaga
      241 acatttgtta aaagaaatta gaacaaaatg agtgaaaaat tagaacaaac aaattcctta
      301 taaaccttat catctcaacc tatattaaga ttttacctag ttgaatcttc ttttctatat
      361 aaagcgtcgg agcatatcag ggggttatct aacgtaaatg ctacccttcg gctcgctttc
      421 gctcggcatt gacgtcagat actgcacccc ctgaaccccc atgctccaac agcaaaaagg
      481 aaactttttg ctgcttttcc gacgcttatt cgcttcgctc atatttatat agaaaagaag
      541 tgaatgcgca aaagacataa tcgattcaca aaaaataggt acacgaaaaa caagttaagg
      601 gatgcagttt atgcatccct taacttactt attaaataat ttatagctat tgaaaagaga
      661 taagaattgt tcaaagctaa tattgtttaa atcgtcaatt cctgcatgtt ttaaggaatt
      721 gttaaattga ttttttgtaa atattttctt gtattctttg ttaacccatt tcataacgaa
      781 ataattatac ttctgtttat ctttgtgtga tattcttgat ttttttctat ttaatctgat
      841 aagtgagcta ttcactttag gtttaggatg aaaatattct cttggaacca tacttaatat
      901 agaaatatca acttctgcca ttaaaaataa tgccaatgag cgttttgtat ttaataatct
      961 tttagcaaac ccgtattcca cgattaaata aatctcatca gctatactat caaaaacaat
     1021 tttgcgtatt atatccgtac ttatgttata aggtatatta ccaaatattt tataggattg
     1081 gtttttagga aatttaaact gcaatatatc cttgtttaaa acttggaaat tatcgtgatc
     1141 aacaagttta ttttctgtag ttttgcataa tttatggtct atttcaatgg cagttacgaa
     1201 attacacctc tgtactaatt caagggtaaa atgccctttt cctgagccga tttcaaagat
     1261 attatcatgt tcatttaatc ttatatttgt cattatttta tctatattat gttttgaagt
     1321 aataaagttt tgactgtgtt ttatattttt ctcgttcatt ataaccctct ttaatttggt
     1381 tataatgaat tttgcttatt aacgattcat tataaccact tattttttgt ttggttgata
     1441 atgaactgtg ctgattacaa aaatactaaa aatgcccata ttttttcctc cttataaaat
     1501 tagtataatt atagcacgag ctctgataaa tatgaacatg atgagtgatc gttaaattta
     1561 tactgcaatc tgatgcgatt attgaataaa agatatgaga gatttatcta gtttcttttt
     1621 ttacaagaaa aaagaaagtt cttaaaggtt ttatactttt ggtcgtagag cacacggttt
     1681 aacgacttaa ttacgaagta aataagtcta gtgtgttaga ctttaatgtt tttttaaggc
     1741 attagtgcat ttaagcgtca gagcatggct ttatgccgag aaaactattg gttggaatgg
     1801 cgtgtgtgtt agccaaagct ttggcgagtt ggttgggggt ttcatgggat taatcccatg
     1861 aaagtaccaa ctcaacaaca cactaacgcc tgttggttcc aaccaatagg aaattggaat
     1921 aagcaattag tataatgttg gtataacgtt agtataatga tgcttttttt cattatattt
     1981 tttatgtact ttaaacctgc acgcttatgt gaattagaaa aagcttaatc gcatttcata
     2041 gattgacctc ccaataacta cgtggtgtta ttgggaggtc aatctatttc atttgcgtta
     2101 agcttttgat ctacattaag cgtgcaggtt ttttgtctat ttgtagacga ttttatgtta
     2161 tactagtcct tgaaagaata ataatcagat aatgcatttt cttgtttttc atttgcctct
     2221 tgctcaaagt tcccaaattc gagtaagagg tatttttgtt tttggtcgtc gcctctcatt
     2281 agtagttcag ggtttaacat taatactcca gtttttcttt ttataatatt tccttcttct
     2341 aagattttaa gtgttgttat tactgtttgt agacttgttc ctgtagcttt tgctatttct
     2401 cttgttgtag ctatcattgt attgttactt aagtggacat tatctaggat atagttaacg
     2461 at
//
