LOCUS       CP154285               13164 bp    DNA     circular BCT 06-MAY-2024
DEFINITION  Staphylococcus aureus strain BSN194 plasmid pNAS017, complete
            sequence.
ACCESSION   CP154285
VERSION     CP154285.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637774
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN194 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13164)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN194
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP043386, CP042347, CP062332, CP014442
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2024 14:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,023
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 43 of 81
            Pseudo Genes (incomplete)         :: 42 of 81
            Pseudo Genes (internal stop)      :: 15 of 81
            Pseudo Genes (multiple problems)  :: 18 of 81
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN194"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pNAS017"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2018-01-03"
                     /collected_by="Andrew D. Berti"
     gene            complement(220..798)
                     /locus_tag="UXQ35_14820"
     CDS             complement(220..798)
                     /locus_tag="UXQ35_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="XAL06827.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(1063..1443)
                     /gene="blaI"
                     /locus_tag="UXQ35_14825"
     ARG             complement(1067..1443)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(1433..3190)
                     /gene="blaR1"
                     /locus_tag="UXQ35_14830"
     ARG             complement(1437..3190)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            3297..4142
                     /gene="blaZ"
                     /locus_tag="UXQ35_14835"
     ARG             3298..4139
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4668..5087)
                     /locus_tag="UXQ35_14840"
     CDS             complement(4668..5087)
                     /locus_tag="UXQ35_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="XAL06831.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            5211..6182
                     /locus_tag="UXQ35_14845"
     CDS             5211..6182
                     /locus_tag="UXQ35_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="XAL06832.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(6354..6446)
                     /locus_tag="UXQ35_14850"
     CDS             complement(6354..6446)
                     /locus_tag="UXQ35_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="XAL06833.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6603..6950)
                     /locus_tag="UXQ35_14855"
     CDS             complement(6603..6950)
                     /locus_tag="UXQ35_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO_process: GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="XAL06820.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(6969..7586)
                     /gene="cadD"
                     /locus_tag="UXQ35_14860"
     ARG             complement(6973..7586)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(8702..8896)
                     /locus_tag="UXQ35_14865"
     CDS             complement(8702..8896)
                     /locus_tag="UXQ35_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XAL06822.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            9006..9698
                     /locus_tag="UXQ35_14870"
     CDS             9006..9698
                     /locus_tag="UXQ35_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="XAL06823.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            complement(9866..10015)
                     /locus_tag="UXQ35_14875"
     CDS             complement(9866..10015)
                     /locus_tag="UXQ35_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XAL06824.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(10562..10851)
                     /locus_tag="UXQ35_14880"
                     /pseudo=""
     CDS             complement(10562..10851)
                     /locus_tag="UXQ35_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_process: GO:0006260 - DNA replication
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(10871..11854)
                     /locus_tag="UXQ35_14885"
     CDS             complement(10871..11854)
                     /locus_tag="UXQ35_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="XAL06825.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            12280..13023
                     /locus_tag="UXQ35_14890"
     CDS             12280..13023
                     /locus_tag="UXQ35_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="XAL06826.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             3298..4142
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(10359..10506)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(10565..10740)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(10872..11854)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12281..13023
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttcattagt gaaacaggta ttatattaaa aaagcgtacg aatttgtacg cttttttctt
       61 gtgttattat taaaatctca taacaggaaa atattatgag attttagacg ctgtcagaac
      121 gtctctttta catcgagtga cattgaatca aagtctttgt atgttaatga tatataaaat
      181 tatttagtag attcattata gtaagctaga ttagttgctc tacttgtttt tattctcttt
      241 tgcctctaat ttattgagat aacgataaat agttgtacgt gaaacttgcc actgctctgc
      301 aattgtttta attggcgtac cattatcata aagtgtctta agtaagttta aatcttgttt
      361 atttaatttt tcaggtctac ctccatatcg gcctctagca cgtgctgcaa ttcgaccggc
      421 tgatgatcgc tctaaaatta aattacgttc aaactctgca aatgctgcga ataaatgaaa
      481 taataattgt cctgtagaac ttgatttatc catcgttata ttttcttcta aactatgaaa
      541 actcacacct cgttcgttaa gttcattaac taacgtgatt aaatcctcca tgttccgtcc
      601 tagtctatct aatctccaaa caacaatcgt atctcctgac ctcgcaaatt caattgcttt
      661 atctaaacca ggtcgcttac tttttgaacc actcatatgg tcattaaata tcttttcaca
      721 accatatgta tttaatcgat cctcctgtaa attcaaattt tgtaatccag ttgaaactct
      781 tgcataacct attttcaaaa tacagacctc ctatatacac ctttagtgta ccataacttg
      841 attacgaggt cttttgtgaa catagatttt ttgttgggtt tttgaacaca taattctatg
      901 taatacttac ttatttagac aaaaaacaag agtgtacaga aaatgggtta ttttctggac
      961 actctcatct ttaataatat attttaaatg tcattaacaa aactaattta ataagagtca
     1021 agtatggttt acatatgtat tatgtttgaa acatacctct aattactttt tactaatatc
     1081 atttaaaatg tctcgcaatt cttcaatttc tttgttattt aattcttcat ttttcgcaaa
     1141 attcagcact aaacttttca tatcccctcc atacagttta ttaagaaagg ttttagcagt
     1201 tttcatttta atatcgtctt ctttaatatt tgatgagtaa aaataaatat tttctgattt
     1261 gtatcgtttt ataatctctt ttttatatag tcttgtgatt aatgttctaa tcgttttatc
     1321 actaacttct ttatattttt gaatttcaac tacaatttca ttagctgata ctgatttttt
     1381 atcccatatt atattcataa catcccattc agccatagat atttcaactt gcttattggt
     1441 catttaaaac acccattcct tttaatattt tttcacttat taattcagca tttttcccag
     1501 acgggtttcc atctgataaa tgtgtagcaa aataatactt atcatgattt gtaattacgt
     1561 aacctacaaa ccacccatta ttatacttcc cgtttactat acctgtacct gttttcccat
     1621 acagttcata cttttcattt ttcttaatca ataatgaaga agataattga ttctttgctt
     1681 ttttactaaa atggttattt tgttccatca tatttttaaa aactattact tgttcaagat
     1741 tagatatttt caaactatct tccatccaat agcttttata acttcccaaa ttttcattac
     1801 cataatttaa ttgcttgagt tgagccgcag tatagttctt tggtatttga ttactaatac
     1861 gttcgaagta ccaattaact gaattttgca ttgctgtatt taaatcttgt tccttattcc
     1921 aagactcgaa aagataatgt ttatgattcc atgacatacg tgaattttta tcacttataa
     1981 tatgacggtc aagtccaaac agtgctaaat aaattttgta agttgaatca ggagaatatc
     2041 tttttctact ttctttttca ttataaatat agtatttgtc tttcttcatg ctgtacataa
     2101 cgaaagatcc actatttgaa ccaaaatttt tactttcatc taaaatttgg taatcgcttt
     2161 gtaagggctt tttataatta taatcagtta aggattgccc cattaaaaac tggctttgaa
     2221 ttatcatgat aaaaaaagta aaaatacaaa taaaaattag aattaatttt gactgctttt
     2281 tcagatcggc ttcttttata ttaattaatc tacttttgag taatgatttt ttaccattaa
     2341 atgaatggct taatatattt ttgttggaaa atggggtttt taatacggag tccataatag
     2401 attccgcata agttttaaat tcatttttgt taatattatt aaggacaaat ctatcggctt
     2461 ctacttcatt gtcatgaatt atctttcttt ttacaatatg tactaatgga ttgtaactca
     2521 taataataga gaagatatta aagataatta aatgtaaagt atctctattt ttagcatgag
     2581 catattcatg cagtattata tattttagtc ttttatcaat tacactttta aaatatgaac
     2641 taggaatcaa aataatatat ttcccatacc aaaaagttat tggagattga atagcctctg
     2701 cttttcgaat cacaatattt tttttatatt gatggttgaa aagtatcgta tctattttat
     2761 ttttttcatt ttcgtttaga taaagtgact gtttctttaa atatttaaga tataataagg
     2821 ctttcaaaaa tttaaaactt aaaataataa ctaaaactat ccaaataact gtgcagatat
     2881 tatcaattga atcccaatta aacttatgga tatctgttgt gaactcttga ataggtttgg
     2941 tggtatttat gttatggttc aaatcgtgtg acttactttc aactgtgggc gcttgattat
     3001 tcacattatt aaattttata aaagagaatt taattgggat aaaaggaatt aatccagcaa
     3061 gcatagttag ataccaaact ttataattta aactataatt aaaatagcgt tttaatatgt
     3121 acctaaaaaa tactaacaat agaaatataa aacaaaagct tactacgctc attattagta
     3181 atttagccat tttgacacct tctttcaaat atttataata aacaattgac atcaatatta
     3241 caattgtaat attattgatt tataaaaatt acaactgtaa tatcggaggg tttattttga
     3301 aaaagttaat atttttaatt gtaattgctt tagttttaag tgcatgtaat tcaaacagtt
     3361 cacatgccaa agagttaaat gatttagaaa aaaaatataa tgctcatatt ggtgtttatg
     3421 ctttaaatac taaaagtggt aaggaagtaa aatttaattc agataagaga tttgcctatg
     3481 cttcgacttc aaaagcgata aatagtgcta ttttgttaga acaagtacct tataataagt
     3541 taaataaaaa agtacatatt aacaaagatg atatagttgc ttattctcct attttagaaa
     3601 aatatgtagg aaaagatatc actttaaaag aacttattga ggcttcaatg acatatagtg
     3661 ataatacagc aaacaataaa attataaaag aaatcggtgg aatcaaaaaa gttaaacaac
     3721 gtctaaaaga actaggagat aaagtaacaa atccagttag atatgagata gaattaaatt
     3781 actattcacc aaagagcaaa aaagatactt caacgcctgc tgctttcggc aagactttaa
     3841 ataaacttat cgcaaatgga aaattaagca aaaaaaataa aaatttctta cttgatttaa
     3901 tgtttaataa taaaaacgga gacactttaa ttaaagatgg tgttccaaaa gactataagg
     3961 ttgctgataa aagtggtcaa gcaataacat atgcttctag aaatgatgtt gcttttgttt
     4021 atcctaaggg ccaatctgaa cctattgttt tagtcatttt tacgaataaa gacaataaaa
     4081 gtgataagcc aaatgataag ttgataagtg aaaccgccaa gagtgtaatg aaggaatttt
     4141 aatattctaa atgcataata aatactgata acatcttata gtttgtatta tattttgtat
     4201 tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt attttcgaga
     4261 tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa aatcataaat
     4321 aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt atcttattta
     4381 aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc aagcaaagtg
     4441 acaatagcat tttacaagta ttttttattg ttcgattagg gtaaacctaa ataatacata
     4501 aattatatta caatatgttt attatagagg ctaaagtatc ttagaaattt ggatagtagg
     4561 tttttcggga ataaaagtat agttggcttt aatatataat taagctaagc tatcatttat
     4621 atttactcga ttataattcg cacttcaact tttttataac gtaaaaatta aaacgtagtt
     4681 ttatttatct ttagtaatat ctttttaaac actacagttt cttcattagt taaattagcg
     4741 aagtaatgat tattaagttg ataggctttc ttttctaatg ttggccataa gtcttttgct
     4801 ttatttgtac tgtttattag cacagttctt ctattttcat tatcagtttg acgtgttatt
     4861 agatcctttt tggacatctt atctaatatt ccagatattg ttgaaggttc taaatttaaa
     4921 gtattaccaa tcgctttagg tgtattcaaa ttactattaa tcaagcaatt caaaacacca
     4981 tattctgctg gagtaatatt atagggactt aataaacttt taaaatgttt aaaaactcta
     5041 ttctgagaag tactcaacaa ataatttata caattatcta attccacaac taaaacctct
     5101 cttaaatcat tagtattgac tcgcttatca tatcatgata agttttgtag gaaaatagtt
     5161 ttgcaacaaa aacatacttc tacaaaacaa ttgtgaaaga aggctaataa atgttcaatg
     5221 cggtagtagt gagaaaaagt gcatcaaatg ttagttataa agtagaaaaa gttgatgaat
     5281 ctcatttatc gaagggtgat gtattagtaa aagtggtata ctcttcaata aattataagg
     5341 atatgctagc tttacaatat aaaggcggtg taattagaga ttatcctatg attcctggta
     5401 tagattttgc aggtattgtt gaatcaagtt cgaatgataa gtttaaagaa ggcgataatg
     5461 tattagttac tggttatgat gttggcgtga cacatacagg tggattttca gaatatgcac
     5521 aagtgcctgg agagtggatt gttcctattc cccaagaaat gtctattaaa gagtctatgg
     5581 tatatggcac agccggtttt acggcagcat tatcaatttt tgaactagaa aaatatggaa
     5641 tgaatatcaa atcacaacct gaaattttag tgtctggggc tacaggtggt gtgggcagtg
     5701 ttgctataca aattttacat aaaattggat atgaaaatat cactgctatc gtaagaaaag
     5761 aaaagcaaat agaaatcgca aaaaaattag gtgcaaaaca agtaataata attgaagatg
     5821 ataataaacc gcttaaaaaa ggtatttttg attatttctt agatacagtg gggagtaatg
     5881 taacattata cggtttaaaa cgtctaaatt atcaaggtgt tgctactgta tgtggaaatg
     5941 tagcaggaaa ttcacttaac gctaatatat taccgtttat tcttagaggt attaaattaa
     6001 ttggcattga ttctgtatat gttgatcata atatccgtga agatatatgg agtaagttag
     6061 caaatgaatg gaatatagga aattcacttc tatacaatga aattggattt gaagaattct
     6121 ataaaacgat agatcagctt ttaaaaggac aacatttagg aagaactatt ttaaaagtat
     6181 aagtgtgagt attcattaat acatttacta ttaattcaat cattttgcat ttctctacta
     6241 accagtgtta taatttatat aacgaaaatg attaacaatt aatgcaccaa tccccacact
     6301 attgcagtag tgtggggatt ttttcgatgc tggcttataa agtcgcctat taattatttg
     6361 ttgcgtttac taagccaata agtataatac gcaacagcac agccactgat gactggtgct
     6421 atgatgtgaa cgaaaatgat tagcaaagta atgcacctcc tctcttcgtc aattgacgcc
     6481 tgagagatag gcgactctat tattatacca tctgtgaata tacaagcata tctacttccg
     6541 ataagtcgtc ttatgttata taactttgtt ttttatagta tcaagacaag aagaaactcg
     6601 tttcaaaaac cctcatgatt tttagagaaa aactctctta tttcatcgtc tttaataaaa
     6661 taatatgcca tctttccatc tttataaaaa tcaagtacct catttttata taaaagcctt
     6721 aaatgatgtg aagttgaagc aacactcatt ttcaatatca aagaaatatc acaaacacac
     6781 aactcatctt ctttaatcaa agataatatg attttcaatt tcttctcatc acaaattttt
     6841 tctaaaatgt taagtaattt cttagattta tcatcttcta aaaaacttaa agcattgtta
     6901 actttatcct catgtacaca gatcacatca caagtatttt cataactcat ataaaatcac
     6961 accctaaatt aaaatataaa tcctaaaatt gtttgaatag tgtcattttc aataataata
     7021 aataaaccta aagctatata aataatagcc ataatccaac gactaaattt ctcaacaatt
     7081 tctccaattc ctggaatatt agctaatttt tgtgcagtaa acaccaagaa gaaaattaaa
     7141 attaaaaaga caaacagagt aagtaataaa ttagtaacac ttaatgtcac aaaatacgga
     7201 acaaataaac caatattatc ggcgccacaa cttgctatcg taacaattgc aaccgtacca
     7261 actaatttag acaatccttt ttcattcaat tcttttttag ctctcttttc tccttcacaa
     7321 tcatcataaa tagccacttt aattccaaga taaattggta ttaaacccaa taaacctaat
     7381 atccactttt ctggaacata atttaataca aaagctaaaa acaaactaac taatattaaa
     7441 ataatagacc ctaaatattg accaacataa atatctctat attcttttct agtctttgct
     7501 ctagcaaaaa atattaataa aattactaat aaatcaactg ctgtagcaat ataaagaaca
     7561 gcagcagcaa caaccgtttg aatcataata cacctcattc aaaaatattt ttgaatgtat
     7621 tataacttat atttttaaat aatcataggt gctttattcc aattgcttta ttgacgttga
     7681 gcctcggaac ccttaacaat cccaaaactt gtcgaatggt cggcttaata gctcacgcta
     7741 tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacatcaata aattttttcg
     7801 gcataaatgc gtgtctatgc gtagacatgg agacacttca gaattatgga gtgattacat
     7861 gagtttaact atttttcttt attcagtttt aggaagttta ttagcaaatt atatctacga
     7921 aaaatcttca aaataaataa ctttttcggt tctactttgt acactttatc tacctggtag
     7981 acctaattgt tattgcttta caggtctaaa attcgtcgtt ataatctagt taaatcccgt
     8041 aagggcgcac ttatacgtcg gataaatcga catgtgcaaa aacgtaatcg accttcgtcg
     8101 atttttttat ttcaaaatta aagaaacggc ttgagatatt ccgagcagtt ataaaaatta
     8161 atttgcgttg aatattgcta ctacattctt gaaaaagtga tgtagattta tagcccataa
     8221 cttttttgaa ttacatcgac gatacggcgt cgaaagaaaa ataaatagac cgtatgaaag
     8281 aaaaagctac ctcactttat ctggggtagc ttttattttt tatgccctta ttttgaattt
     8341 taagagggca ttttaaagat ttaggggtga ttcatatagt tttatatcta aaacttatat
     8401 atgcttttaa aacgcaaata tgagccaaat aaatatatgc tattttcaaa aacaaaaatt
     8461 tgagcaaaaa caataactat ttttagacac tgcccattta catgcaaatt aaaaattagc
     8521 ataaaattct ttaaaacttc cccacgtttt tctaaattcc tgtagggcgt ttttatcaaa
     8581 aatcaagaca agaagaaact cgttcacact cgtttcaaaa aaacaagaaa tctaatctat
     8641 acacctaccg ttttccaacc ccctcaaagc atacaaatta aaaacaaaat tattcataat
     8701 cttactttct tttaacaaaa acataaaaac cataaataga caaattaact atcaaaggca
     8761 ataaacagta taaaaaaacc acgaataaat cttcatcaac atctaaaata aaaaagaata
     8821 aaacaattaa caaaatacta attaacaaac ctaaaagtgg caaacttttt atatattttt
     8881 ctaaattttc attcataaaa tagactcctt tcaaaaatat gtttataaat atattaaaat
     8941 aaatattgat tttataaagc gcttacttat acaattaaaa tatcttatta tcttggaggt
     9001 atcaaatgaa aattataaaa ttattttcaa taatttcctt gttcctactg gtatttagcg
     9061 cgaatttttc taatgcttac gcaaatgaag aacaaaaaag ttctttacta gaaaatcaaa
     9121 aagaaaaatt gaaaagcaaa aacatcgaag tatcacaaat gaccaagaaa gaaataagtt
     9181 ttctagaaca attgcaaaat aaaaatgaag atatgcaaaa attcgaaaag caaatgaaaa
     9241 aagaaggatt taaaaaaata aagctagaag acaaaaattc taaattaaaa tatctagcta
     9301 cagaagcaaa tgataaaaat gcatatggct acatagctaa taacgcttat aaaaatgaaa
     9361 aaacaaaaga aattgtagtt tctgaaacaa tatatgataa ctacaacaaa aaaatcacaa
     9421 aattcattgc cgaaaaaaga agtatgactg aaaacaaaga gggaaaaata cttattaaca
     9481 aaaattacgt tgaatataag gctgaaaaag ttgataaaaa cagaccggta acatatgcag
     9541 gattcaaatg gaacggcaaa gcattcgctt gtagtatggc tggactttat gcatgtgctc
     9601 actactgtgg agtatgggca atcgtaaatc caatagccgg cggtgtatgt gaagcagttt
     9661 gcggaactgc atttgcagct ggatgctctg taggttgatt ttattccaat tgttttattg
     9721 acgttgagcc tcggaaccct taacaatccc aaaacttgtc gaatggtcgg cttaatagct
     9781 cacgctatgc cgacattcgt ctgcaagttt agttaagggt tcttctcaac atcaataaat
     9841 tttttcggca taaatacgat gttccttatt tatcctcata aaaaacaata gctgaaatat
     9901 ataaccaatt actgtgtgtt tgaatttcta atacatgaat atcattcttt aaaaacctat
     9961 taacttgttt atctaattga cgatgagtaa aagtagcgaa agttttaatc ttcatgatat
    10021 ctctcctttt ataaaaatag caatccttat tacaatatta aacactttta ttttggattt
    10081 taagaggcat tttgaagact tagaggcaaa tcatataggt tttgtactta aaaatctata
    10141 cagtctctta aaacgcaaat atgagccaaa taaatatatg ctaatttcac aaacaaaaat
    10201 ttcagcaaaa tcagtgacta attttttaga tactgcccat ttacatgcaa atttaaaaat
    10261 tagcataaaa tactttaaaa cttccccatg tttttctaaa ttcctgcagg gcttttctta
    10321 tcaaaaactt agaaatttta atttaactct aaaatctccg taagagcata agggaaattt
    10381 gctatcattc aaaaatgata gatctggcaa ggccagacat tccaagggtt ttaagttatt
    10441 ttaaagtgat atcatacaaa gctgtagtgt cacacttttg tgacactaca tacagcattt
    10501 gtcacaaaaa taagacagca cttttttaca gaaaaacaat aaaaaaagac atcagttatc
    10561 actaatgtct taaataataa ttttgctttt cgaaattcca aattgcggta attgaatgtg
    10621 ctttttttct tcgtctgtcg ttttatcttc atcacttgta ttaatcaaat taccatcttc
    10681 ggcatcgtct aagtttaaaa ttttatgctt ttgtttaagc aatccaccat aacttaacat
    10741 acgttttcga tacaaacctt tttctaaatc acttacaatt tcttgatttt tttcgtcgtc
    10801 atcagttaaa aagtcagatg atttaacgga atattttgat gtctcttcga gtgttataaa
    10861 atcatttgaa ttattgatta aagatattat attgtttcat gtttttacgg cgcatataaa
    10921 gggcatgtgt ttcttcaagt tcagttagaa ttgtttgcat taaataagct tgcattgact
    10981 tgattgtttc cttttggcct ttaactcccc tatcatgtat agctttttta agtcttttta
    11041 aagaagttaa tatttcatcc tcaatgtcct ctaacatgta atgcatatct tcatgccttg
    11101 aattaaatga tttttttgct ttaagaataa cagcctttat gatttctaaa tctctataat
    11161 catattttaa taaataatta gttagttgtt ctgggaattc ttgcaatagc atttctttat
    11221 catcaaaatt atgtgtaaat tgatttgaat gatttgaatg attactaatt tcatttttgt
    11281 actttatatc attcaagtca ttcatatcat tattctcagt atcattaaag tcaatgtcat
    11341 taaagtcagt atcattagtg ttcgattttc gaacttctgg acgttcgatt ttcgaacttc
    11401 tggacgttcg attttcgaac ttctggacgt tcgattttcg aacttccttc tcgccgtaag
    11461 gttcaacatc gttttcttct ttttctattt tgtaaatatc acgttctgtg acaataggtt
    11521 tcattaaata tagaatattt ggtttattta aaccacgtcg tttttgaatg agtagaccat
    11581 cgttttctaa cccttttttt atttttgtta acttttcttt tttacaattt aagatttcca
    11641 ttagcttttc atttgaataa acaaagtaaa tgttaccatc ttcatcaacc cagctatttt
    11701 taatagataa atttaagcga tcacgtaaga tagcataggc tattttcata tcattagtta
    11761 gatttttata attttcactg gtaaagaata ctttagggat ttgataaaat ctttctttgt
    11821 aattttcttg cactgtaaaa tattgatttt gcatgacaaa aaacctcttt ccatatttta
    11881 gtttggtaag aggtaataaa acgtttttat ttagctttac atttatttaa tgatttaggt
    11941 gcttgcacta agatattatt cattatataa tacaagtatc gaaaattata taaataaaca
    12001 gtttctaaac gttttattac caaaccccga ctattaccag tagttggggt ttttgttata
    12061 ttaagtttat tatagcactt taaataaact tataaaaggg aaaatatagc tttatcaagg
    12121 gtttataaac ccttgataaa tatcataaac ctttcataaa cccttgataa aaaggttgat
    12181 ggatgtttat ttttatttat taatcacttt attattaagc attataagct tataatattt
    12241 tatcaagggt ttataaacat tttataaagg gttgatgata tgaaaagtgt gaaaaagttg
    12301 agtgaagaat taggggttag taagcaaacg atttttaata atattaagcg attgaatata
    12361 gagacaataa aacaggaaaa cacttcgttt attaaagaag ataccgatat agaaaaaatc
    12421 attcaaagag taaatgaaaa taaaaagaaa tatggttttg aaagtactac tgaagacaaa
    12481 caaaaaaaag ggtcggataa tattaatttt gaatcgaaaa gcgatgctca aatagtagaa
    12541 atccttaaaa atcagattga tactttaaat aatcaaattg aaaaacaaga aagtcgtcat
    12601 gaaacaacaa ttgaatttta cagaaaagag ttgcaagaaa gatcaaaatt gcttgaaaat
    12661 cagcaagttc tggcattaga gagtaataaa aagattcaaa aattagaaaa tcaattagaa
    12721 aaagaaaaac accttaatta tccctttgat acatctacta acactagaca aaacgttgat
    12781 gcacaagaaa aaacatatac tactaatcct gtaaatatta acagagatca ggaagaaact
    12841 aaagaaacag agattcaata taaggatata tctggaagtc aaagcgacga aagtacacaa
    12901 ggtgaagaag caaaaaaaga agacgttcca gctaatctaa atgacaatga tagtgatata
    12961 gaagaaaaga gtgaagaaac ggaacctaaa aaggggtttt ggagtcgttt gtttggtagc
    13021 taaaataatt gattaggcac taaaagttat aaaattttag tgtcttttat atagtgagaa
    13081 cttctggtaa tcatcgatta tgtaaactaa agtatttcaa ataattaact gtattaatac
    13141 tctcatcatg attgcgttat aaac
//
