LOCUS       CP152460                2456 bp    DNA     circular BCT 01-MAY-2024
DEFINITION  Staphylococcus aureus strain BSN132 plasmid pSR42, complete
            sequence.
ACCESSION   CP152460
VERSION     CP152460.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637710
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN132 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN132
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP030326, CP092053, CP042087, MN216340
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2024 11:36:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,819
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 44 of 80
            Pseudo Genes (incomplete)         :: 38 of 80
            Pseudo Genes (internal stop)      :: 28 of 80
            Pseudo Genes (multiple problems)  :: 28 of 80
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN132"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSR42"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-08-28"
                     /collected_by="Andrew D. Berti"
     gene            complement(join(2157..2456,1..177))
                     /locus_tag="UXQ16_14495"
     CDS             complement(join(2157..2456,1..177))
                     /locus_tag="UXQ16_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="XAH95968.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(624..1358)
                     /gene="erm(C)"
                     /locus_tag="UXQ16_14500"
     ARG             complement(628..1358)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1403..1462)
                     /gene="ermCL"
                     /locus_tag="UXQ16_14505"
     CDS             complement(1403..1462)
                     /gene="ermCL"
                     /locus_tag="UXQ16_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="XAH95970.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(625..1358)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(2..177)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.06e-87"
     Typing          complement(2158..2453)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.564"
                     /coverage="99.0"
                     /evalue="6.14e-77"
ORIGIN
        1 tttaagtttt tttccgccaa tcatatctaa catacttatt aattgcacta tatatgcctt
       61 tacgaagtta ccagacgttt gtttacggta taacttgtct acctctatga cttctccact
      121 ttcttcgtct atgagcctct gagagccttt atagactgtt ccatatcttt ctttcatctt
      181 tttctcactc cttattttaa actattctaa ctatatcata actgttctaa aaaaaaagaa
      241 catttgttaa aagaaattag aacaaaatga gtgaaaaatt agaacaaaca aattccttat
      301 aaaccttatc atctcaacct atattaagat tttacctagt tgaatcttct tttctatata
      361 aagcgtcgga gcatatcagg gggttatcta acgtaaatgc tacccttcgg ctcgctttcg
      421 ctcggcattg acgtcagata ctgcaccccc tgaaccccca tgctccaaca gcaaaaagga
      481 aactttttgc tgcttttccg acgcttattc gcttcgctca tatttatata gaaaagaagt
      541 gaatgcgcaa aagacataat cgattcacaa aaaataggta cacgaaaaac aagttaaggg
      601 atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
      661 aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
      721 ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
      781 taattatact tctgtttatc tttgtgtgat attcttgatt tttttctatt taatctgata
      841 agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
      901 gaaatatcaa cttctgccat taaaaataat gccaatgagc gttttgtatt taataatctt
      961 ttagcaaacc cgtattccac gattaaataa atctcatcag ctatactatc aaaaacaatt
     1021 ttgcgtatta tatccgtact tatgttataa ggtatattac caaatatttt ataggattgg
     1081 tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
     1141 acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
     1201 ttacacctct gtactaattc aagggtaaaa tgcccttttc ctgagccgat ttcaaagata
     1261 ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
     1321 ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttgctt
     1381 attaacgatt cattataacc acttattttt tgtttggttg ataatgaact gtgctgatta
     1441 caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata attatagcac
     1501 gagctctgat aaatatgaac atgatgagtg atcgttaaat ttatactgca atctgatgcg
     1561 attattgaat aaaagatatg agagatttat ctagtttctt tttttacaag aaaaaagaaa
     1621 gttcttaaag gttttatact tttggtcgta gagcacacgg tttaacgact taattacgaa
     1681 gtaaataagt ctagtgtgtt agactttaat gtttttttaa ggcattagtg catttaagcg
     1741 tcagagcatg gctttatgcc gagaaaacta ttggttggaa tggcgtgtgt gttagccaaa
     1801 gctttggcga gttggttggg ggtttcatgg gattaatccc atgaaagtac caactcaaca
     1861 acacactaac gcctgttggt tccaaccaat aggaaattgg aataagcaat tagtataatg
     1921 agagtataat gttggtataa cgttagtata atgatgcttt ttttcattat attttttatg
     1981 tactttaaac ctgcacgctt atgtgaatta gaaaaagctt aatcgcattt catagattga
     2041 cctcccaata actacgtggt gttattggga ggtcaatcta tttcatttgc gttaagcttt
     2101 tgatctacat taagcgtgca ggttttttgt ctatttgtag acgattttat gttatactag
     2161 tccttgaaag aataataatc agataatgca ttttcttgtt tttcatttgc ctcttgctca
     2221 aagttcccaa attcgagtaa gaggtatttt tgtttttggt cgtcgcctct cattagtagt
     2281 tcagggttta acattaatac tccagttttt ctttttataa tatttccttc ttctaagatt
     2341 ttaagtgttg ttattactgt ttgtagactt gttcctgtag cttttgctat ttctcttgtt
     2401 gtagctatca ttgtattgtt acttaagtgg acattatcta ggatatagtt aacgat
//
