LOCUS       CP152459                2827 bp    DNA     circular BCT 01-MAY-2024
DEFINITION  Staphylococcus aureus strain BSN132 plasmid pSALNP58, complete
            sequence.
ACCESSION   CP152459
VERSION     CP152459.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637710
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN132 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN132
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP030326, CP092053, CP042087, MN216340
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2024 11:36:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,819
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 44 of 80
            Pseudo Genes (incomplete)         :: 38 of 80
            Pseudo Genes (internal stop)      :: 28 of 80
            Pseudo Genes (multiple problems)  :: 28 of 80
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN132"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSALNP58"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-08-28"
                     /collected_by="Andrew D. Berti"
     gene            2..574
                     /locus_tag="UXQ16_14470"
                     /pseudo=""
     CDS             2..574
                     /locus_tag="UXQ16_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]; GO_process: GO:0006313 - DNA transposition [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(630..1028)
                     /locus_tag="UXQ16_14475"
                     /pseudo=""
     CDS             complement(630..1028)
                     /locus_tag="UXQ16_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /name="DegV family protein"
     gene            complement(1038..1523)
                     /locus_tag="UXQ16_14480"
     CDS             complement(1038..1523)
                     /locus_tag="UXQ16_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrS1"
                     /protein_id="XAH95967.1"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMAR
                     KTFNSIGKPLPNRRNVVLTNQASFHHEGVDVINSLDEIKELSGHVFIFGGQTLYEAMID
                     QVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKGK"
                     /name="trimethoprim-resistant dihydrofolate reductase
                     DfrS1"
     gene            complement(1565..2521)
                     /locus_tag="UXQ16_14485"
     CDS             complement(1565..2521)
                     /locus_tag="UXQ16_14485"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004799 - thymidylate synthase activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="XAH95966.1"
                     /translation="MYNPFDEAYHGLCEEILEIGNRRDDRTHTGTISKFGHQLRFDLTK
                     GFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYVQSDDYHGPDM
                     TDFGHRSQQDPEFNEQYKEEMKKFKERILNDDAFAKKYGNLGNVYGKQWRDWEDKNGNH
                     YDQLKSVIQQIKTNPNSRRHIVSAWNPTEIDSMALPPCHTMFQFYVQEGKLNCQLYQRS
                     ADIFLGVPFNIASYALLTHLVAKECGLEVGEFIHTFGDAHIYSNHMDAIHTQLSRDSYL
                     PPQLKINTDKSIFDINYEDLELINYESHPAIKAPIAV"
                     /name="thymidylate synthase"
     gene            2635..2826
                     /locus_tag="UXQ16_14490"
                     /pseudo=""
     CDS             2635..2826
                     /locus_tag="UXQ16_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     ARG             complement(1042..1523)
                     /name="dfrS1"
                     /gene="dfrS1"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrS1"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1039..1523)
                     /name="dfrC"
                     /gene="dfrC"
                     /gene_name="trimethoprim resistant dihydrofolate reductase
                     dfr"
                     /tool="rgi"
                     /drug_class="diaminopyrimidine antibiotic"
                     /identity="98.14"
                     /coverage="100.0"
ORIGIN
        1 attaagggga cgtggtgtaa acgttcatca ttcaacggtc taccgttggg ttcaagaata
       61 tgccccaatt ttatatcaaa tttggaagaa aaagcataaa aaagcttatt acaaatggcg
      121 tattgatgag acgtacatca aaataaaagg aaaatggagc tatttatatc gtgccattga
      181 tgcagaggga catacattag atatttggtt gcgtaagcaa cgagataatc attcagcata
      241 tgcgtttatc aaacgtctca ttaaacaatt tggtaaacct caaaaggtaa ttacagatca
      301 ggcaccttca acgaaggtag caatggttaa agtaattaaa gcttttaaac ttaaacctga
      361 ctgccattgt acatcgaaat atctgaataa cctcattgag caagatcacc gtcatattaa
      421 agtaagaaag acaaggtatc aaagtatcaa tacngcaaag aatactttaa aaggtattga
      481 ntgtatttac gctctatata aaaagaaccg cagntctctt cagatctacg gattttcgcc
      541 atgccacgaa attagcatca tgctagcaag ttaagcgaac actgacatga taaattagtg
      601 gttagctata tttttttact ttgcaacaga accaattcaa caatttgctt aatttgataa
      661 cctaacccaa acgaaataga tttagaatca atcactgtaa tattaccatc taccatatgg
      721 ctcgcttgaa cagcagtatt ataagtacca cttaagcctg acgaaagatg aatacttata
      781 atttcaacgt catcttgagc taattgctca tatgtttcaa taaatcgacc aataggtggt
      841 tgacttgttt tgacgtctgc atcattttca atatgatcga tatactcact tgaagagata
      901 tcaacttgat cagtgtaaga tttcccgtcg attgtcaggc ttaaaggtat aacatgaatg
      961 ttatgttgtt ttaaatattc atgtgataaa tcagaggttg aatccgtgac gataatttgt
     1021 ttagccataa ttgcctccta tttccctttt ctacgcacta aatgtaagaa tgtatgcggt
     1081 atagtatttt tttcatctag ttgaccttct actgaagatt cgacttccca gttttcgaat
     1141 gtgtatggtg gaaagaatgt gtctccttga aactttccat ctattactgt gatatacata
     1201 tcatctacct ggtcaatcat tgcttcgtat aacgtttgtc ctccaaatat aaaaacatga
     1261 ccagataact ctttaatttc atcaagagag tttataacat ctaccccttc atggtgaaat
     1321 gaagcttggt tagtgagtac gacgttacgt ctatttggca atggcttccc tatagaatta
     1381 aaagttttcc gtgccattac aagtgtattc ccagtggtca gttgtttaat atgctttaaa
     1441 tcatttggta agtgccaagg taattgattt tggtacccaa tgactctttg tttatcgtga
     1501 gcgacaatta ttgataatgt catggtatac ctcctttctg tatgcgaaat attaatgcat
     1561 ctttttatac cgcaataggt gctttaatag cagggtgtga ttcataattt atcaattcta
     1621 aatcttcata attaatatca aaaattgatt tatctgtatt aatttttaat tgaggtggta
     1681 gataactatc tcttgataat tgtgtatgta ttgcatccat atgattggaa taaatatgtg
     1741 catctccaaa tgtatgaatg aattcaccca cctcaagtcc acattctttt gcaacaagat
     1801 gtgtcagtaa agcataactt gcaatattga aaggaacacc taaaaatata tcagcacttc
     1861 tttgatataa ttgacaattt aatttaccct cttgtacgta aaattgaaac attgtgtggc
     1921 aaggtggtaa tgccattgaa tcgatttcag taggattcca tgctgaaaca atgtgacgtc
     1981 gagagtttgg attggtttta atttgttgta taactgattt taattgatcg taatgattcc
     2041 catttttatc ttcccaatct ctccattgtt taccataaac attccctaag ttgccatatt
     2101 ttttagcaaa tgcatcatcg tttagaatac gttctttaaa ttttttcatt tcttctttat
     2161 actgctcgtt aaattcaggg tcttgctggg aacgatgtcc aaaatctgtc atgtctggtc
     2221 catgataatc atcagactga acataatttt caaatgccca ttcattccat atattgttat
     2281 tatatttaag taggtattga atgtttgtat ctcctttaat aaaccataat aattcagttg
     2341 ctactaattt aaaagaaacc tttttggttg taagtaaagg aaaccctttg gttaaatcga
     2401 aacgcaattg atgcccaaat ttggaaattg tcccggtatg tgtgcgatca tcgcgtctat
     2461 tccctatttc taatatctct tcgcataagc catgataagc ttcgtcgaat ggattataca
     2521 tgcgtacaac tccttataaa aatatgtact atattaataa acattatact agaaaagagg
     2581 ttctgttgca aagttgaatt tatagtataa ttttaacaaa aaggagtctt ctgtatgaac
     2641 tatttcagat ataaacaatt taacaangat gttatcactg tagccgttgg ctactatcta
     2701 agatatgcat tgagttatcg tgatatatct gaaatattaa ggggacgtgg tgtaaacgtt
     2761 catcattcaa cggtctaccg ttgggttcaa gaatatgccc caattttata tcaaatttgg
     2821 aagaaaa
//
