LOCUS       CP151888                2491 bp    DNA     circular BCT 27-APR-2024
DEFINITION  Staphylococcus aureus strain BSN163 plasmid pSE4, complete sequence.
ACCESSION   CP151888
VERSION     CP151888.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637743
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN163 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN163
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP043386, CP014445, CP014442, CP066372
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/18/2024 08:56:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,686
            Genes (coding)                    :: 2,606
            CDSs (with protein)               :: 2,606
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 42 of 80
            Pseudo Genes (incomplete)         :: 38 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 17 of 80
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN163"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSE4"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-11-18"
                     /collected_by="Andrew D. Berti"
     gene            125..313
                     /locus_tag="UXR07_13655"
     CDS             125..313
                     /locus_tag="UXR07_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="WZX47179.1"
                     /translation="MSEEKFEQAKGNIKETIGDATDNKDLKAEGKGDKASGKAKEVVDD
                     VKDKANDLIDKVKGDKN"
                     /name="CsbD family protein"
     gene            958..1902
                     /locus_tag="UXR07_13660"
     CDS             958..1902
                     /locus_tag="UXR07_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WZX47180.1"
                     /translation="MSRKEQEIHSKQSTLQEVTNYTSTGYSNSRLGVHSVHPQNPKLSF
                     DAMTIVGNLSRDNAQKLSSFMSIEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIINYMEDDGFTRIDLAFDFEDDLSNYYAMTDKAVK
                     KTVFYGRDGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVMSDHLWRVEIELKRDMV
                     DYWNDCFNDLHILKPDWTSPEKLNEQAMVYMLIHEEDKWGELNKRTKYKYKNLIKEISP
                     VDLTELMKSTLRENEKQLQKQIEFWQTKGKKLF"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          1055..1877
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.204"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tgtatacatg ggataataca aggtttaatg catgggataa aaaaggatat gatttcactt
       61 ttaaaaaggg tatttgaata atagaattgt attgtagaca ttgattattt aggaggaata
      121 gaaaatgagc gaagaaaaat tcgaacaagc aaaaggaaat atcaaagaga ctattggaga
      181 tgctactgat aataaggatt taaaagcaga aggtaaagga gataaagctt ctggtaaagc
      241 caaagaagta gtagatgatg ttaaagataa agctaatgac ttgattgata aagttaaagg
      301 agataaaaac taaataaaaa ttaaaacgtc ttaagtatta ttatgtagca tacttgggac
      361 gttttttatt taaagtgaat aaaaataaat aataagacac gcatttatgc cgagaaaatt
      421 tattgatgtt gagaagaacc cttaactaaa cttggagacg aatgtcggca tagcgtgagc
      481 tattaagccg accattcgac aagttttggg attgttaagg gttccgaggc tcaacgtcaa
      541 taaagcaatt ggaataaagc aacaaaagga gttgaagaaa atgagtgaga aaactaagaa
      601 aagcattcgt atagcattat cttttgcaca agtagtagta cagtcattta tagtttatta
      661 tgcttacaag caatataaat tatctaaaga aaattaattt ttgattatgt aataagtttt
      721 ggtactaaaa aagacttgat ctgattagac caagcctttt gatagtgtta tattaataaa
      781 caaataaaaa gaagtcgctc acccaccgac caaagtttgt gaacgacaat ccaaaaagaa
      841 aacatgagtt gttttttatg aatttgtata tttaaatact aaacgatatt taaatataca
      901 tcaagataaa tattcgggtg ggcgacttct taaattaaat taaggagtcg attttttatg
      961 agtagaaaag agcaagaaat tcattcaaag caaagcacat tacaagaagt tacaaattac
     1021 acctcaaccg gatactctaa tagccggtta ggagtgcatt ctgtgcaccc acaaaatcct
     1081 aaattaagtt ttgacgctat gacaatagtt ggaaatctca gtcgtgacaa cgctcaaaag
     1141 ctatcgtcat ttatgagtat cgaaccacaa attcgacttt gggatatact acaaacgaaa
     1201 tttaaagcta aagcactgca ggaaaaagtt tatatcgaat acgacaaagt aaaagcagat
     1261 acatgggata gacgtaatat gcgtgttgaa tttaatccga ataaacttac gcatgaagaa
     1321 atgctttggt taaaacaaaa cattatcaac tacatggaag atgacggttt tacaagaata
     1381 gatttagctt ttgattttga agatgattta agtaattatt atgcaatgac tgataaagca
     1441 gttaagaaaa ctgtatttta tggtcgtgac gggaaaccag aaacaaaata ttttggcgtt
     1501 cgtgatagtg atagatttat tagaatttat aacaaaaaac aagaacgtaa agataacgca
     1561 gatgtcgaag ttatgtctga tcacttatgg cgtgtagaaa ttgaacttaa aagagatatg
     1621 gtggattact ggaatgattg ttttaatgat ttacatatct tgaaaccaga ttggacttcg
     1681 ccagaaaaat taaatgaaca agcaatggtt tacatgttaa ttcatgaaga agacaaatgg
     1741 ggagaattga ataaaagaac taaatataaa tataaaaatt taattaaaga aatatctcca
     1801 gttgatttaa cggaattaat gaaatcgact ttaagagaaa atgaaaaaca attacaaaag
     1861 caaatagaat tttggcagac aaaaggtaag aagctatttt aaaaatggct accatttgac
     1921 tcccttttag ctcccaaatg gctccataaa aagggagcta tttgggagct atttttgatt
     1981 ttatgtagcc atttgggagt catttttgat tttgggtagc tattcggtag cttttgtgta
     2041 gtcgttcggg agtcatataa caatttttat cgcataaata ggaatcccat ttgacactgt
     2101 caaatcaata ttttgcacag aaacggctac cgaatggcta catacggaga catttctgtg
     2161 taggattcct cttatgctct tttaaaatat tttaaaatat aaaagttaca ttatacacct
     2221 atttttttca ggttttagct ttttattgag aaggataatt aaaataaaaa aacatatttt
     2281 tttctgaaag tgttgagaaa taaattttaa aatgatttat agcctctctg ttgaacaaaa
     2341 aaagttttta gtcattttta tacgtggaat gattaaatgt cctagaaagg gcttaaaatg
     2401 caaaataaaa agactactca ttttttgagt agtctatctg taggatagtt tttgatattc
     2461 taaatgaaaa ggagtaatac ataaatcatt t
//
