LOCUS       CP151825                3239 bp    DNA     circular BCT 24-APR-2024
DEFINITION  Staphylococcus aureus strain BSN119 plasmid pUSA01, complete
            sequence.
ACCESSION   CP151825
VERSION     CP151825.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637697
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN119 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN119
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP014445, CP014442
            Genome Coverage                   :: 99.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/18/2024 11:00:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,926
            CDSs (total)                      :: 2,844
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 48 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 16 of 88
            Pseudo Genes (multiple problems)  :: 18 of 88
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN119"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pUSA01"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-09-11"
                     /collected_by="Andrew D. Berti"
     gene            1..1110
                     /locus_tag="UXR02_14455"
                     /pseudo=""
     CDS             1..1110
                     /locus_tag="UXR02_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            1693..1881
                     /locus_tag="UXR02_14460"
     CDS             1693..1881
                     /locus_tag="UXR02_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WZW09005.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2207..2788)
                     /locus_tag="UXR02_14465"
     CDS             complement(2207..2788)
                     /locus_tag="UXR02_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WZW09006.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1181..1345
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          2..749
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          932..1102
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="91.0"
                     /evalue="6.2e-68"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgattttttt catcatcatc agttaaaaaa tcagatgatt
      781 taaccgaata ttttgaggtc tctttgattg ccgattcaat gtctttatcg ccttttctat
      841 tcggttaaat catctgattt tttaactgat gatgatgaaa aaaatcaaga aattgtaagt
      901 gatttagaaa aaggtttgta tcgaaaacgt atgttaagtt atggtggatt gcttaaacaa
      961 aaacataaaa ttttaaactt agacgatgtc gaagatggta atttgattaa tgcaagtgat
     1021 gaagataaaa caacagacga agaagaaaaa gcacattcaa ttaccgcaat ttggaatttc
     1081 gaaaagcaaa attattattt aagacattag tgttgactaa tgtctttttt gttgattttt
     1141 tataaaaaag tactgtctta tttttgtgac aaatgctgta tgtagtgtca caaaaataag
     1201 acaaacgcaa tatattgtgt cacaaaaata agacagtaca gctttgtatg atatcacttt
     1261 aaaatacctt aaaacccctg gaatttctgg ctttgccaga cctatcattt ttgaatgata
     1321 gcaaattctc cttatgctct tacggagttt ttagagaaaa attaaaaatt ctcgattttt
     1381 gataaaaaac gccctgcagg aatttagaaa aacatgtgga agttttaaag gattttatgc
     1441 taatttttaa tttgcatgta aatgggcagt gttaaaaaat tagtcactgg ttttgctgaa
     1501 atttttgttt gtgaaattac catatattta tttggctcat atttgcgttt taagagcttg
     1561 tatatatttt taagttaaaa acctatatga taccccctaa acctttaaaa taccccctta
     1621 aaactcaaaa taaaggtatt tataatttga gtaagtcttg tgataaagtt ttttaaaaaa
     1681 ggagtgttta ttttgaataa aaacacagtg ttagatgaag gttacgttat agcaactatt
     1741 ttaaatgttt tctttttact tggattaatt tttatatctc atttggaaaa tttatatata
     1801 ttaattccgt atgttatttt gatgggaata aatgctattt atttggttgt taaggttatg
     1861 aatttcaaaa agaataatta gaatatttga ggggattttg aaacgagttt cttcttgttt
     1921 tcacactgtt tttttattcc tattggtgtt gttgcttact ttttgttttt ctataaagat
     1981 gatgcttttg acgagatgga agaaaaatat gattatcatg aagataataa aaaatagacg
     2041 acgcatttat gccgagaaaa tttattgatg ttgagaagaa cccttaacta aacttgcaga
     2101 cgaatgtcgg catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa
     2161 gggttccgag gctcaacgtc aataaagcaa ttggaataaa tagtatctaa agtaacccgt
     2221 atttaagatt acattgtaca tccttcgaaa ccatagtagc tgcgattcca gctaggtttc
     2281 cttttatccc aattttagct aatttttttg ctacgccctt taagtttttg gctttaacat
     2341 cttcaaaaac tgctgtgatt atgtttccag ttaaaagttc tccatatgag tttaaaactt
     2401 cagaatagaa acagtcaccg ctatttttgt agttcccacc aactgctcta ggtgatattg
     2461 aactagtttt ctttttattc tctatgtcgt tttttagttt ttggaatcct tcaggaacat
     2521 atccatatcg agatttggca ttttctagat taacatcttt agctgttccg tctttatcta
     2581 aaataataat tttgccatat atgtatttta gttctttttc aatttctttt tcgtttaaag
     2641 tttcaacgtt tttatctgca atgttatttt tgttttgggc ttgagcttct ttgaaattaa
     2701 aatccaagac cattgaaagt atggttaaaa tacttaaaga tactactaaa atctttctta
     2761 gtgatatact gttttgaatt gcattcattg taaatgcccc cttgttttgt tatttaaatg
     2821 aattcaatat aatattaaca tataaaaaca aaggagttta atatgaataa gtttttttac
     2881 tttttgatta ttttattatc tgtattttta atttttaatt ttgttaattc gattttattt
     2941 gataatcctt ttgacttttt agaaagtttt atttattcat tggttttcac attaatttat
     3001 ttaggtttag agggtcataa aaagaagaag gaataggttg ttttttgaaa cgagtgtgaa
     3061 cgagtttctt cttgtcttga tactatatag aaataactcg attttatata tatagctgta
     3121 actgttgata ttacagtgtt taaacgtgtt tttgtgcgtg aaaggaaaat ttgacaataa
     3181 aaaaccccag ttatattatt aaggtgtcga atcttaaata atactggggg tctttttat
//
