LOCUS       CP151663                9294 bp    DNA     circular BCT 23-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2023EP-00287 plasmid unnamed3, complete
            sequence.
ACCESSION   CP151663
VERSION     CP151663.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN38335504
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Stanton,R. and Wang,W.
  TITLE     Long read sequencing of 2023EP-00287, KPC-3 producing Klebsiella
            pneumoniae ST219
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Stanton,R. and Wang,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2024) Division of Healthcare Quality Promotion,
            Centers for Disease Control and Prevention, 1600 CLIFTON RD,
            Atlanta, GA 30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/16/2024 12:04:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,527
            CDSs (total)                      :: 5,398
            Genes (coding)                    :: 5,237
            CDSs (with protein)               :: 5,237
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 17
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 56 of 161
            Pseudo Genes (incomplete)         :: 106 of 161
            Pseudo Genes (internal stop)      :: 35 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023EP-00287"
                     /isolation_source="body fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(join(8136..9294,1..527))
                     /locus_tag="SLJ68_27585"
     CDS             complement(join(8136..9294,1..527))
                     /locus_tag="SLJ68_27585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WZU14331.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            930..1349
                     /locus_tag="SLJ68_27590"
     CDS             930..1349
                     /locus_tag="SLJ68_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WZU14332.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1655..1918
                     /locus_tag="SLJ68_27595"
     CDS             1655..1918
                     /locus_tag="SLJ68_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WZU14333.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            1908..2207
                     /locus_tag="SLJ68_27600"
     CDS             1908..2207
                     /locus_tag="SLJ68_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WZU14334.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2269..2646)
                     /locus_tag="SLJ68_27605"
     CDS             complement(2269..2646)
                     /locus_tag="SLJ68_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WZU14335.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            2715..2894
                     /locus_tag="SLJ68_27610"
     CDS             2715..2894
                     /locus_tag="SLJ68_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WZU14336.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2922..3452)
                     /locus_tag="SLJ68_27615"
     CDS             complement(2922..3452)
                     /locus_tag="SLJ68_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WZU14337.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3459..4190)
                     /locus_tag="SLJ68_27620"
     CDS             complement(3459..4190)
                     /locus_tag="SLJ68_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WZU14338.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(4190..6154)
                     /locus_tag="SLJ68_27625"
     CDS             complement(4190..6154)
                     /locus_tag="SLJ68_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WZU14339.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7635..7784)
                     /locus_tag="SLJ68_27630"
     CDS             complement(7635..7784)
                     /locus_tag="SLJ68_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WZU14329.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7869..8126)
                     /locus_tag="SLJ68_27635"
     CDS             complement(7869..8126)
                     /locus_tag="SLJ68_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WZU14330.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDCFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6954..7532
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3463..4190)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gtctccgtta ccgtgtctgc cggcaatgac gtcacgattt ttgacatcat tttcggatca
       61 tcttttgcta tctcagaagg cagcactccg taaatcgcaa tcccccacag accaaactta
      121 aacggacctt tcagggcagc cagcacatca gcaacagcgg ccgacaacgc atcgccggaa
      181 acggataaag ccagtccttc agcccccgga gtggccaagg caggcagacc gaaggccatc
      241 gcggtggccg aagactgtcc gccaccagag ctcccgctat gattgccgct gttaccgcca
      301 ttaccaccgg gtttactccc gccgttacca aactcccccg ggttatagtt atgaatatcc
      361 ccgttaggtg tatgcgtcgt accccagccc ggcccgctgt ttggatccgt accaccacct
      421 gatgaagggc cacctttacc tgatgtaccg ttcacattcc cactggcctg accaccattg
      481 tgtcccagac ctgaattacc cggacctcgt ccgtctccgc cactcatata cttttcctct
      541 ttgactttta aaacaataaa ctaaaataaa atactgtaca tataaccact ggttttatat
      601 acagtataaa aattacgctg ccttgttttt catgtcaagt ctgtaaattt gcgtttttgt
      661 gaaaatgatc aaaaaaacag ccactcacac catgcgcaat cccctccaga tgcaacaaaa
      721 accagcacaa acggcgccgt tctccggcct cagtatgttt tactgaaatc attatatgag
      781 ataccagaaa gccccctccg ggacaggaaa agaaacgggg ggtctggttc gggttgttcg
      841 gtcactgaac acggtcagaa actgatgata agatagcctg cggatatata actcaatgag
      901 aaaaaagaat taaaggtaag gagacgctca tgagacgttc cggcagcaaa ctgtgtctta
      961 tcgccatgat aacagcgaca gcaactctca ctgcctgtgc tccaaagggc agcgtggaac
     1021 agcatacccg gcattacgta tatgcttcag atgacggttt tgatcccaat ttttccaccc
     1081 agaaagctga cacaacacga atgatggtgc cttttttccg gcagttctgg gatatgggcg
     1141 caaaggataa agcgaccgga aaatcacgga gtgatgtgca gcaacgcatt cagcagtttc
     1201 acagtcagga atttttaaac tcactccaag gcacaacaca atttgcgggt accgattacc
     1261 gcagcaaaga cctcacaccg aaaaaatcaa ggctactggc tgacaccatt tcagctgttt
     1321 atctcgatgg ctatgaaggc agacagtaag tggatttacc ataacgcgtc attacgcgca
     1381 ccgctcagac gggctcaggg cgtttccggc ggctacggga taaaacaggc aacaaccccc
     1441 ccggaaaact gccggaaacg cgcccgataa attttatcct aatgcatgcc tatgcacgca
     1501 actgcatggc atatgttttg acgtaaaatg gatgccgtaa gccctttttt accgactttc
     1561 tgcttcatca gcctgactga cacgaccata ttctttcaga ccgccccctg ctaaacttcc
     1621 ttcatattgc cgcattaacc cgggaggctc cgcaatgagc aacactacac actacgaaaa
     1681 cgccaacttt cttcgggaac tggctgaaag cctgccccgc atactgccgg aaggtggccc
     1741 ggacaaagct gcgctgttgc agcgactggc taacgaagaa ctggcccagg cagagtatga
     1801 agatcaggtt cgcgccaaag tgaccgccgc ccgtgctgac acgcgtccgg gtatgaccac
     1861 ggaacaactc cgccagcgcc tgcatggccg ttatcaggaa ctgcgcgatg ccgtatgacg
     1921 tggaatggaa gcagggagcc atcgaggatg taacgacgct gtttgactat atcgcggaaa
     1981 attcatccct gtgggatgcg cagaacgtta ccgaccgggt tctcgctgcg gcggacaaac
     2041 tggccgactt cccgcgactc tatgaagtgg actcacgcta tggcgaggac gttcgccgga
     2101 tcagtctgat gggacagcat gtcctctatg atgttgatga ccagaccaga aaagtccggg
     2161 ttctggccgt ggtcggccag cgccagaatc cccatattgt tctctaataa ccggtgacgg
     2221 ggacgcaatt cattgcgtgg ccgtcgccgg ttggacaagc acagcgcgtc agcgttttcc
     2281 ccacggccac caccggcgcg gtgttccggc cggggtctct gctgtctccg gtttgtgttc
     2341 cagcagtctc atggactgca tttcctgccg tagcccggcc acctcctccc tgagtgcttt
     2401 tatctccgcg atcaacgcgt cggaggccat tttttcagtc tcacccgaca gtctcaactc
     2461 gtcccgctcg tgagtctcat cctgtcttaa ctcaccgtac acccggataa gctcgctggt
     2521 atccaccacc cggccgccat cagcttccgt acggtatgat acgcggcctt tcgccatatc
     2581 ccgatacagg gaacttcggg ctttacccgt cagttccgtc gcctgtctca ccgtcaccca
     2641 cgccattgtc tcaccccgct atttttgaga cagtatacct gacaggcgca caccatgccc
     2701 ggaaggaaag aatcatgaca aaacaggaaa aaacagccct caatatggct aaatttatta
     2761 aggagcagtc gctgctattg ctcgataaac tgaatgagct ggatatggat accccggccg
     2821 acatgtgcga gcggctacac gaggatgcgg aaacactctg gcaggtgatg cgggataagc
     2881 tcggggagga ataacagcac cggcatggcc ggtgctggca tttatttttt gccgaacggc
     2941 caccaccggc gggcaggttt gtcctccggc agttcaggta ccggcacgtt aaaatagcgg
     3001 ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc
     3061 cgctcactca ccacgtcaat catcgcgtta atctgctccg gtgagcactt cccggcatca
     3121 accacctgca caccgttctc gatggcgaac cgctccacct tgcgatagag gtaatcacac
     3181 agcttcattc ctgtgggttt gttccgttcc cattgcggat ttttaccagt gaaaaagtac
     3241 gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc
     3301 agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc
     3361 accatatctt ccagatgctc gctggtcggc atgatgcagc gctcgccgtt ccggtcctcc
     3421 gcgtcttcaa taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta
     3481 aagcacgcaa acaaccgttc acgatccagc tctgccgttt tgcgggcctc gccggtctgt
     3541 gggttcacga tttcatccac cgacaggatt gcccgctgca ccctgacctg cgtcgcccgg
     3601 tccgggcaga tgtaccagat ttcatcgatg ccgttagctt tgcggttgaa cagatacccg
     3661 gacaccactt cctgataccg gcgacggctc ttgacggtgc gctccacctc aaacgccacc
     3721 gttttaccgt ccagctccgc cagggcgtcc ggcactttca tgcctttaag actcagacgg
     3781 tgcagaatgc gccagccggt ggcccctctg gcttccatat caaggcgcag ggattgcacg
     3841 gccagccggt gcggcagatg tgctgccgac acccttgacg gctgaaacgg aataacatcc
     3901 gccagcggtt cgtcctcgtc gaacgagaac gccacgccat gcggcgtcag cccccacacg
     3961 ctgacctgac ggccgtccac cggatatttg gtccggacca gcagcccttt cgccaccagg
     4021 ctgtcgagga agcggtagac cggctgcacc gccgcaatcc ccagccgccg cgcaaccagc
     4081 tccgccgtgg aacacagctc ttcccgcaac cagcgcaggg tcagccgggt tttctccgcg
     4141 ttccgcgcct gtcgtttcgc gtggctgacg ttgtacagct ccagcgccat cagtaaatcc
     4201 ccgcaaaatc atcatccgct ttcggcccgc cgccgtcggc cgggggccgg aactccgcca
     4261 ggggatcggt tttcgccagc accgggaaac gctgcaacgg gatttcccgg cgctcaaccc
     4321 gcagcggact gctgaacgcc atccgggcca cacccagccc ggtcatgacc gcgaacccgt
     4381 ccggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc
     4441 tgtccccgct gatatgctcc acgttccccg cctcccggct ggcacggcgg cgctccacgt
     4501 ccaccctgat ttccccggtc tgccccgccg cccactgcgc ggactccagg ctggccgccc
     4561 ggtaaaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaat tcagcgggca
     4621 gatcctgccc gcactgcccg aaatcgccca gcgactggtg cgcgagcaac agattgcagt
     4681 cccggctgcg cagcgtcccc agtgcgttca gtacatactt cgacagcaga tatttaattt
     4741 catcgagcat cacgctgacg tgtggccact cccggaactc gtcccgcgca atgatgattt
     4801 gcgcgcagcg ggcaaacagc attttttgca cacggatcac cgcctcgtcg tccatcgagc
     4861 cgataacgta caggcagccg ccgccgttga taatccccgc cacatcgatg ccactgtccg
     4921 tctgcaggac gctcaggtca gcgacgtttt ccagcctagt gatcaacccc ttcacgtcct
     4981 ttttcagggc ctccggcagc gcgtacgccg ccgccagcac ctgctgaatg tttgccccct
     5041 gcggaaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg
     5101 ccacgtcgcc ggtctcgccc aggttaagcc cggcaatcag caggtttttc agcgcgtact
     5161 ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg
     5221 tgaagtccgg acagtgcgcc cggaagacgt gcggcgccca tgcgtcccct ttggggtcga
     5281 agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatca
     5341 cgctcttgcc gctcccgggc aggccgagga tctgaatgtt ggtcttgcgc caggtatcac
     5401 gcggaaccag caccggcttg ccctgctcat ccttacccag aaacaccgcc tgctcctgcc
     5461 gggcctgctc cgaggaaaaa tcggccagcg cattaaccgg taccggctcc ccgaaatgca
     5521 ggtggcgcac gtcggtcatc tctcctgtcc gctggctgct gcgccggctc tcctgattca
     5581 gtttcgcgtt caccgcctcg tcagcagccg caaaatgacc gtcaagcagc aggaacgcca
     5641 gcagccgcgc caacacaatc ccgacagcca gcccgcccgc gcaccaggca atcccgaaca
     5701 ggttagccgg ctgcagcagg taataatctg cgtttgcgta gaggtccgga cgcaggacgg
     5761 tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg
     5821 ccgcgccggt cagccagtac gcgtagttcc agacatgtcg acgtgccctg accatccgcc
     5881 cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta tcgcgcttat
     5941 agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc atccgtccgg
     6001 cccaaagttc agccagaccg aaaaacaggc cgataactag gaaagccccg gccagaccgt
     6061 aatactcctg gtacagccag ctgacccacg gataaatctg ccgggccagc tccggaatgt
     6121 gccacttcca ggcaagcgaa tccgtattaa acatgttctc tctccttcag acgatggttt
     6181 gccggcgtga tgtgcagccc ccgctgcaca agccgcggac atgccccaag gcatggcggc
     6241 ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac ggaggccagc ctctttccgc
     6301 cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg tgtgagagga
     6361 aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga gttcgccata
     6421 gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgcca ggtgcgtcca
     6481 gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg tgaatttctg
     6541 gcgtggccgg cgtccagccg gacggagcca gaaagagggg gagagaccgt gcgtcggtgg
     6601 cgtccagcca gcgtaagcag gggatatccc cctgaggtca gccacgccct cgcggggatg
     6661 acctgtattt ctccgtaagg agaaaaaagc aacagagata ggttaacgta aaatgttcag
     6721 cgaaaaaccg atatgattca cctatcctgg taatttatta tcagggaata taaagactta
     6781 atcaggggac aatatgccac atcagcgcgt ccggtgtccg tcttgtaggt gccggggaaa
     6841 ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt gagactgcgg cgggcgctgc
     6901 ggacacatgc aaagttaccc acagattccg tgaataagca ggggactaac atgtgaggca
     6961 aaacggcaag gccacgcagg tggcgttttt ccataggctc cgccctcctg actgagttca
     7021 cataaacaga cgctttttcg gtgcatctgt gggagccggt ggctcaacca tgaatcagtc
     7081 aggagtggcg aaacccgaca ggacttaaag atcatcaccg tttccgatga atcgctccct
     7141 cttgcgctct cctgttccaa ccctgccgtt tcccggatac ctgttccgcc tttctccctt
     7201 gcgggaagtg tggcgctttc tcatagctca cactctggta tctcggctcg gtgtaggtcg
     7261 ttcgctccaa gctgggctgt aagcaagaac cccccgttca gcccgactgc tgcgccttat
     7321 ccggtaactg ttcacttgag tccaacccgg aaaagcacgc aaaacgccac tggcagcagc
     7381 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
     7441 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
     7501 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
     7561 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     7621 tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga cgatggtgcc
     7681 accgttccac cctgaacatc ccggatatag tttgcctgac atgcgaccag aagaaagcct
     7741 gaaaaaatca atataaaaag aaaaacaacc tttacctttt tcatatacct tccctgtatt
     7801 taaaaacccc ggtattcatc cctgaacacc ggaagtctgt atttcaaatc atccttgata
     7861 acttttgctt accagttacc atcccggtaa acaaaggata caaagcaatc aaatttacta
     7921 atatcgatta catttttaaa gtgtggctgt aatatcgaaa cccatgattt ttcaacatca
     7981 aaccaaccat tattaatatt atcctttaaa ggcatcccca gactttcaat gacagaacca
     8041 tcatcaccga agtcttttga gtattcacca cctttaaact cttcggtttt cttatcaaac
     8101 caatgaatat gtaatttaag ccccatattt acctcttaaa gatatttttt aatgtttcgt
     8161 tttggatccg gccctttaac ctgcttaccc gtttttggat cgaatgcccc gaggtgttcg
     8221 ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc ataaatttta
     8281 cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt aggggtttta
     8341 ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg atcaggaaaa
     8401 taatcatggc cgtaatcttt agttccctta cgaggttttt tcttttcctc atttaattta
     8461 ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggaaagc
     8521 gcagccttat ccctggataa ggattcattc atttcgttta aagatgtctg ggcagctttt
     8581 aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg
     8641 gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct
     8701 tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggatcgc cttctctgtc
     8761 gatgcaatgc gactgtttaa ggtcgcaata tttttatctt cagcatccag ctcagctttc
     8821 gctttatcat actctctttt cagcccctct tccgggtgag cagcgtccca tgcctgctgg
     8881 cgacgctttt cttcctccag acgctgtttt acctgtgccg gagtaagaac atccgtcacc
     8941 gacacataga ccggtttctg tccgctctct ttcgggaaac gaataacggc ttcacgggag
     9001 ttaccaccgg ccgtaaaacc agccggacgt gaatcacctt tttcagcaat aatgcctttt
     9061 ggcggggctt tcgcggccgg aacaccttta ggtacgctca cctgcagaga cgggagaccc
     9121 ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacaggaaca
     9181 ctcattggcc ggccggtgac aaccgcaata tgctgccgct cgtccttcac aatatccgcc
     9241 acacgtttag tgacgctgac cgtcgcctgg tccagcggca gggagctgac cggc
//
