LOCUS       CP151565                2896 bp    DNA     circular BCT 21-APR-2024
DEFINITION  Staphylococcus aureus strain BSN126 plasmid pSa2594-4, complete
            sequence.
ACCESSION   CP151565
VERSION     CP151565.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637704
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN126 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN126
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP030326, GQ900404, CP027493, CP014447,
            CP035293, CP043301
            Genome Coverage                   :: 97.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/15/2024 11:16:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,071
            CDSs (total)                      :: 2,989
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 44 of 88
            Pseudo Genes (internal stop)      :: 29 of 88
            Pseudo Genes (multiple problems)  :: 28 of 88
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN126"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSa2594-4"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-09-19"
                     /collected_by="Andrew D. Berti"
     gene            join(2811..2896,1..4)
                     /locus_tag="UXR65_15225"
                     /pseudo=""
     CDS             join(2811..2896,1..4)
                     /locus_tag="UXR65_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(join(2893..2896,1..86))
                     /locus_tag="UXR65_15230"
                     /pseudo=""
     CDS             complement(join(2893..2896,1..86))
                     /locus_tag="UXR65_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(201..515)
                     /locus_tag="UXR65_15235"
     CDS             complement(201..515)
                     /locus_tag="UXR65_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO_process: GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WZO01167.1"
                     /translation="MKSSSTSPLTNDSIQEVSKIFKMISDPTRLSILFLLQKEELSVGV
                     IAQSLSMEQSAISHQLKTLKTSRLVKSKRVGKNMIYSLDDLHIFSILEQVSTHIGEQEK
                     "
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            769..2688
                     /locus_tag="UXR65_15240"
     CDS             769..2688
                     /locus_tag="UXR65_15240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005524 - ATP binding [Evidence IEA]; GO_function:
                     GO:0015662 - P-type ion transporter activity [Evidence
                     IEA]; GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]; GO_function: GO:0019829 - ATPase-coupled
                     monoatomic cation transmembrane transporter activity
                     [Evidence IEA]; GO_function: GO:0046872 - metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WZO01168.1"
                     /translation="MKEIQEQHSHENNSHDHDHDHGKMPIILYFIGLALALIAFFLSKE
                     YQILQNILFLIATISAGYHVIVLEGLGETIHNSKSQKRFIPNSHILMGLAAIGASLMGN
                     FGEGALLIFIFSGAHFLEDYAEGRSRREITKLLEMNPTTARLILPDGSTKNVKVNELKV
                     GNHLQVLNGDQVPIDGIILSGSTSIDESSINGESIPKEKSKGDEVFGSTINGTGTFTME
                     VTKENKDTXFSKILQLVNQNQDNQTKAASIIQKFEPKYVTFVLVAILLFILLTPFLLDW
                     TWSQSFYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYLSQLADIQAIA
                     FDKTGTLTKGKPEVTNHYFTDSMDEEEIIDIVVALEKNSNHPLADAILRKFEPKNQLSI
                     EVENQIGKGLSGDYNGRKYRIGKPTSFDDVSDEYVRLNNQWASEGKTVVYLAVDEEVIG
                     LIALMDVPSKYAKETIEYFNKQGIHTTLITGDSEMTGKAVASKLGIDEVIANVMPDEKS
                     KIIDKQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEVSDLVLMQNNLTKLT
                     QSHKISTKMNRLIWQNIIFSMAVVAFLIIVSLVGLTDIAISVIVHEGSTLVVILNGLRL
                     LNSK"
                     /name="heavy metal translocating P-type ATPase"
ORIGIN
        1 cgataactca atgcatatct tagatagtag ccaacggcta cagtgataac atccttgtta
       61 aattgtttat atctgaaata gttcatacag aagactcctg actccttttt gttaaaatta
      121 tactataaat tcaactttgc aacagaaccc tttatttgaa ctaatgaatt gtaaaataat
      181 tttaactttg tagtttgtgc ttatttttct tgttctccga tatgagttga aacttgctca
      241 agaatactaa aaatatgaag atcgtcaagg ctataaatca tatttttacc aactcttttt
      301 gattttacta atcttgaagt ctttaaagtt tttaactggt gagaaatggc tgattgttcc
      361 atacttaaag actgcgcaat tactccaaca ctaagttctt ctttctgtaa aagaaataaa
      421 attgaaagac gtgtagggtc actaatcatt ttaaatatct tacttacctc ttggatagaa
      481 tcatttgtta acggagaagt tgatgatgat ttcataaaac ttcctctctt tcatatgtgt
      541 gttcgttcat gtttaggtta gcacaagatt tttataaagc aaggtttttc cataagcaga
      601 gctgttttat agtccaattt ttttattaac taaataagat aactattttt ttgacaaatc
      661 agatagagca atatataatg aacgcataaa catatgaatg ttatttcata tgtaattcgt
      721 aagcgttacg atttcaaaaa aatagtttta ttaagaaggg tgatagaaat gaaagagatt
      781 caagagcaac actcacacga aaataatagt cacgatcatg accatgatca tgggaaaatg
      841 ccaattattt tgtactttat tggtttagca ctagcattga tagctttctt tttgagtaaa
      901 gagtatcaaa tcttacaaaa tattttgttc ttaatcgcta ctataagcgc tgggtaccat
      961 gttattgtcc ttgaaggact tggggagacg atccataact caaaaagtca aaaaagattt
     1021 atacctaatt ctcatatctt aatgggatta gcagcaattg gagcatcttt gatgggaaat
     1081 tttggagaag gtgcgttatt aatctttatt ttttctgggg cacattttct agaagattat
     1141 gcagaaggaa gaagtagacg agaaattaca aaattactcg aaatgaatcc cacaactgct
     1201 cgtttaattc tcccagacgg tagcacaaaa aatgtgaaag ttaatgaatt aaaagttgga
     1261 aatcaccttc aagtattaaa tggtgatcaa gtccctattg atggaattat tttgtctgga
     1321 tctacgtcta ttgatgagtc ttctattaat ggagagagta taccaaaaga aaagtccaag
     1381 ggagacgaag tttttgggag cacaataaat ggaacgggca cttttacaat ggaagtcacg
     1441 aaagaaaata aagatacggk gttttcaaaa attttacaat tagtgaatca aaaccaggat
     1501 aaccaaacta aagctgcaag tattattcaa aaatttgaac caaaatatgt aacatttgtt
     1561 ttagttgcca tattactatt tatattatta actccatttc tacttgattg gacatggtca
     1621 caaagctttt atagaggatt ggttctttta gttgcagctt cgccatgtgc tttggcagca
     1681 gctactgtat cggcaacttt atcaacaact tctaatttag ctaaaaaagg cgttctttct
     1741 aaggggagtt cttatctatc tcaattagct gatattcaag ctattgcatt tgataagacg
     1801 ggaactttaa ccaaaggaaa acctgaagtc acgaatcatt attttactga ttctatggat
     1861 gaagaagaaa taattgatat tgtagtagct cttgagaaaa attccaatca tcctttagcg
     1921 gatgctattt taagaaaatt tgaaccaaaa aaccaacttt ctattgaagt agaaaatcag
     1981 attggaaaag gattgtccgg agactataat ggtcgaaaat atcgtatagg taaaccaact
     2041 tcttttgacg acgtctcaga tgaatatgtt cgattaaata atcaatgggc atctgaaggg
     2101 aaaactgtcg tatatctagc agtagatgaa gaagttatag gccttattgc acttatggat
     2161 gtaccaagta aatatgctaa agaaactatt gagtacttca ataaacaagg tattcatact
     2221 acgttaatta caggtgattc ggaaatgaca ggaaaagcag tcgcaagcaa gctaggtata
     2281 gacgaagtga ttgccaatgt catgccagat gaaaaatcta aaattattga caaacaaaaa
     2341 gaaaaatatg gtgtgaccgc aatggtagga gatggtgtaa atgatgctcc agctcttgtc
     2401 aatgcagatg taggaatagc catgggagat ggaactgatg tggcagtaga ggtttctgac
     2461 ttagtattaa tgcaaaataa cttaacgaaa ttaacacagt ctcataaaat ttctacgaaa
     2521 atgaatcgtt taatttggca gaatattatt ttttcaatgg cagttgtagc ttttctaatt
     2581 atcgtgagct tagtaggatt aacggatata gccattagtg tgatcgttca tgaaggaagt
     2641 acattagtcg taatattaaa tggtctccga ttattaaatt ctaaatgaat acatgaaatt
     2701 ggataaaatc gtgtatcgag aatcctatac ctatagggtt cttttttggt tctgttgcaa
     2761 agttgaattt atagtataat tttaacaaaa aggagtcagg agtcttctgt atgaactatt
     2821 tcagatataa acaatttaac aaggatgtta tcactgtagc cgttggctac tatctaagat
     2881 atgcattgag ttatcg
//
