LOCUS       CP151561                2445 bp    DNA     circular BCT 21-APR-2024
DEFINITION  Staphylococcus aureus strain BSN93-2 plasmid pSR42, complete
            sequence.
ACCESSION   CP151561
VERSION     CP151561.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637681
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN93 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN93
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP030326, MN216340, CP042087
            Genome Coverage                   :: 177.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/15/2024 09:58:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,977
            CDSs (total)                      :: 2,895
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 31 of 88
            Pseudo Genes (multiple problems)  :: 31 of 88
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN93-2"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSR42"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-08-08"
                     /collected_by="Andrew D. Berti"
     gene            complement(join(2146..2445,1..177))
                     /locus_tag="UXQ76_14875"
     CDS             complement(join(2146..2445,1..177))
                     /locus_tag="UXQ76_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WZN91940.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(624..1358)
                     /gene="erm(C)"
                     /locus_tag="UXQ76_14880"
     ARG             complement(628..1358)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1403..1462)
                     /gene="ermCL"
                     /locus_tag="UXQ76_14885"
     CDS             complement(1403..1462)
                     /gene="ermCL"
                     /locus_tag="UXQ76_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WZN91942.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(625..1358)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(2..177)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.06e-87"
     Typing          complement(2147..2442)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.564"
                     /coverage="99.0"
                     /evalue="6.14e-77"
ORIGIN
        1 tttaagtttt tttccgccaa tcatatctaa catacttatt aattgcacta tatatgcctt
       61 tacgaagtta ccagacgttt gtttacggta taacttgtct acctctatga cttctccact
      121 ttcttcgtct atgagcctct gagagccttt atagactgtt ccatatcttt ctttcatctt
      181 tttctcactc cttattttaa actattctaa ctatatcata actgttctaa aaaaaaagaa
      241 catttgttaa aagaaattag aacaaaatga gtgaaaaatt agaacaaaca aattccttat
      301 aaaccttatc atctcaacct atattaagat tttacctagt tgaatcttct tttctatata
      361 aagcgtcgga gcatatcagg gggttatcta acgtaaatgc tacccttcgg ctcgctttcg
      421 ctcggcattg acgtcagata ctgcaccccc tgaaccccca tgctccaaca gcaaaaagga
      481 aactttttgc tgcttttccg acgcttattc gcttcgctca tatttatata gaaaagaagt
      541 gaatgcgcaa aagacataat cgattcacaa aaaataggta cacgaaaaac aagttaaggg
      601 atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
      661 aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
      721 ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
      781 taattatact tctgtttatc tttgtgtgat attcttgatt tttttctatt taatctgata
      841 agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
      901 gaaatatcaa cttctgccat taaaaataat gccaatgagc gttttgtatt taataatctt
      961 ttagcaaacc cgtattccac gattaaataa atctcatcag ctatactatc aaaaacaatt
     1021 ttgcgtatta tatccgtact tatgttataa ggtatattac caaatatttt ataggattgg
     1081 tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
     1141 acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
     1201 ttacacctct gtactaattc aagggtaaaa tgcccttttc ctgagccgat ttcaaagata
     1261 ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
     1321 ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttgctt
     1381 attaacgatt cattataacc acttattttt tgtttggttg ataatgaact gtgctgatta
     1441 caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata attatagcac
     1501 gagctctgat aaatatgaac atgatgagtg atcgttaaat ttatactgca atctgatgcg
     1561 attattgaat aaaagatatg agagatttat ctagtttctt tttttacaag aaaaaagaaa
     1621 gttcttaaag gttttatact tttggtcgta gagcacacgg tttaacgact taattacgaa
     1681 gtaaataagt ctagtgtgtt agactttaat gtttttttaa ggcattagtg catttaagcg
     1741 tcagagcatg gctttatgcc gagaaaacta ttggttggaa tggcgtgtgt gttagccaaa
     1801 gctttggcga gttggttggg ggtttcatgg gattaatccc atgaaagtac caactcaaca
     1861 acacactaac gcctgttggt tccaaccaat aggaaattgg aataagcaat tagtataatg
     1921 ttggtataac gttagtataa tgatgctttt tttcattata ttttttatgt actttaaacc
     1981 tgcacgctta tgtgaattag aaaaagctta atcgcatttc atagattgac ctcccaataa
     2041 ctacgtggtg ttattgggag gtcaatctat ttcatttgcg ttaagctttt gatctacatt
     2101 aagcgtgcag gttttttgtc tatttgtaga cgattttatg ttatactagt ccttgaaaga
     2161 ataataatca gataatgcat tttcttgttt ttcatttgcc tcttgctcaa agttcccaaa
     2221 ttcgagtaag aggtattttt gtttttggtc gtcgcctctc attagtagtt cagggtttaa
     2281 cattaatact ccagtttttc tttttataat atttccttct tctaagattt taagtgttgt
     2341 tattactgtt tgtagacttg ttcctgtagc ttttgctatt tctcttgttg tagctatcat
     2401 tgtattgtta cttaagtgga cattatctag gatatagtta acgat
//
