LOCUS       CP151560                2827 bp    DNA     circular BCT 21-APR-2024
DEFINITION  Staphylococcus aureus strain BSN93-2 plasmid pSALNP58, complete
            sequence.
ACCESSION   CP151560
VERSION     CP151560.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637681
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN93 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN93
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP030326, MN216340, CP042087
            Genome Coverage                   :: 177.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/15/2024 09:58:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,977
            CDSs (total)                      :: 2,895
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 31 of 88
            Pseudo Genes (multiple problems)  :: 31 of 88
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN93-2"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSALNP58"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-08-08"
                     /collected_by="Andrew D. Berti"
     gene            complement(2..193)
                     /locus_tag="UXQ76_14850"
                     /pseudo=""
     CDS             complement(2..193)
                     /locus_tag="UXQ76_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            307..1263
                     /locus_tag="UXQ76_14855"
     CDS             307..1263
                     /locus_tag="UXQ76_14855"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004799 - thymidylate synthase activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="WZN91938.1"
                     /translation="MYNPFDEAYHGLCEEILEIGNRRDDRTHTGTISKFGHQLRFDLTK
                     GFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYVQSDDYHGPDM
                     TDFGHRSQQDPEFNEQYKEEMKKFKERILNDDAFAKKYGNLGNVYGKQWRDWEDKNGNH
                     YDQLKSVIQQIKTNPNSRRHIVSAWNPTEIDSMALPPCHTMFQFYVQEGKLNCQLYQRS
                     ADIFLGVPFNIASYALLTHLVAKECGLEVGEFIHTFGDAHIYSNHMDAIHTQLSRDSYL
                     PPQLKINTDKSIFDINYEDLELINYESHPAIKAPIAV"
                     /name="thymidylate synthase"
     gene            1305..1790
                     /locus_tag="UXQ76_14860"
     CDS             1305..1790
                     /locus_tag="UXQ76_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrS1"
                     /protein_id="WZN91939.1"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMAR
                     KTFNSIGKPLPNRRNVVLTNQASFHHEGVDVINSLDEIKELSGHVFIFGGQTLYEAMID
                     QVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKGK"
                     /name="trimethoprim-resistant dihydrofolate reductase
                     DfrS1"
     gene            1800..2198
                     /locus_tag="UXQ76_14865"
                     /pseudo=""
     CDS             1800..2198
                     /locus_tag="UXQ76_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /name="DegV family protein"
     gene            complement(2254..2826)
                     /locus_tag="UXQ76_14870"
                     /pseudo=""
     CDS             complement(2254..2826)
                     /locus_tag="UXQ76_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]; GO_process: GO:0006313 - DNA transposition [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /name="IS6-like element IS257 family transposase"
     ARG             1306..1787
                     /name="dfrS1"
                     /gene="dfrS1"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrS1"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1306..1790
                     /name="dfrC"
                     /gene="dfrC"
                     /gene_name="trimethoprim resistant dihydrofolate reductase
                     dfr"
                     /tool="rgi"
                     /drug_class="diaminopyrimidine antibiotic"
                     /identity="98.14"
                     /coverage="100.0"
ORIGIN
        1 ttttcttcca aatttgatat aaaattgggg catattcttg aacccaacgg tagaccgttg
       61 aatgatgaac gtttacacca cgtcccctta atatttcaga tatatcacga taactcaatg
      121 catatcttag atagtagcca acggctacag tgataacatc cttgttaaat tgtttatatc
      181 tgaaatagtt catacagaag actccttttt gttaaaatta tactataaat tcaactttgc
      241 aacagaacct cttttctagt ataatgttta ttaatatagt acatattttt ataaggagtt
      301 gtacgcatgt ataatccatt cgacgaagct tatcatggct tatgcgaaga gatattagaa
      361 atagggaata gacgcgatga tcgcacacat accgggacaa tttccaaatt tgggcatcaa
      421 ttgcgtttcg atttaaccaa agggtttcct ttacttacaa ccaaaaaggt ttcttttaaa
      481 ttagtagcaa ctgaattatt atggtttatt aaaggagata caaacattca atacctactt
      541 aaatataata acaatatatg gaatgaatgg gcatttgaaa attatgttca gtctgatgat
      601 tatcatggac cagacatgac agattttgga catcgttccc agcaagaccc tgaatttaac
      661 gagcagtata aagaagaaat gaaaaaattt aaagaacgta ttctaaacga tgatgcattt
      721 gctaaaaaat atggcaactt agggaatgtt tatggtaaac aatggagaga ttgggaagat
      781 aaaaatggga atcattacga tcaattaaaa tcagttatac aacaaattaa aaccaatcca
      841 aactctcgac gtcacattgt ttcagcatgg aatcctactg aaatcgattc aatggcatta
      901 ccaccttgcc acacaatgtt tcaattttac gtacaagagg gtaaattaaa ttgtcaatta
      961 tatcaaagaa gtgctgatat atttttaggt gttcctttca atattgcaag ttatgcttta
     1021 ctgacacatc ttgttgcaaa agaatgtgga cttgaggtgg gtgaattcat tcatacattt
     1081 ggagatgcac atatttattc caatcatatg gatgcaatac atacacaatt atcaagagat
     1141 agttatctac cacctcaatt aaaaattaat acagataaat caatttttga tattaattat
     1201 gaagatttag aattgataaa ttatgaatca caccctgcta ttaaagcacc tattgcggta
     1261 taaaaagatg cattaatatt tcgcatacag aaaggaggta taccatgaca ttatcaataa
     1321 ttgtcgctca cgataaacaa agagtcattg ggtaccaaaa tcaattacct tggcacttac
     1381 caaatgattt aaagcatatt aaacaactga ccactgggaa tacacttgta atggcacgga
     1441 aaacttttaa ttctataggg aagccattgc caaatagacg taacgtcgta ctcactaacc
     1501 aagcttcatt tcaccatgaa ggggtagatg ttataaactc tcttgatgaa attaaagagt
     1561 tatctggtca tgtttttata tttggaggac aaacgttata cgaagcaatg attgaccagg
     1621 tagatgatat gtatatcaca gtaatagatg gaaagtttca aggagacaca ttctttccac
     1681 catacacatt cgaaaactgg gaagtcgaat cttcagtaga aggtcaacta gatgaaaaaa
     1741 atactatacc gcatacattc ttacatttag tgcgtagaaa agggaaatag gaggcaatta
     1801 tggctaaaca aattatcgtc acggattcaa cctctgattt atcacatgaa tatttaaaac
     1861 aacataacat tcatgttata cctttaagcc tgacaatcga cgggaaatct tacactgatc
     1921 aagttgatat ctcttcaagt gagtatatcg atcatattga aaatgatgca gacgtcaaaa
     1981 caagtcaacc acctattggt cgatttattg aaacatatga gcaattagct caagatgacg
     2041 ttgaaattat aagtattcat ctttcgtcag gcttaagtgg tacttataat actgctgttc
     2101 aagcgagcca tatggtagat ggtaatatta cagtgattga ttctaaatct atttcgtttg
     2161 ggttaggtta tcaaattaag caaattgttg aattggttct gttgcaaagt aaaaaaatat
     2221 agctaaccac taatttatca tgtcagtgtt cgcttaactt gctagcatga tgctaatttc
     2281 gtggcatggc gaaaatccgt agatctgaag agacctgcgg ttctttttat atagagcgta
     2341 aatacactca atacctttta aagtattctt tgctgtattg atactttgat accttgtctt
     2401 tcttacttta atatgacggt gatcttgctc aatgaggtta ttcagatatt tcgatgtaca
     2461 atggcagtca ggtttaagtt taaaagcttt aattacttta accattgcta ccttcgttga
     2521 aggtgcctga tctgtaatta ccttttgagg tttaccaaat tgtttaatga gacgtttgat
     2581 aaacgcatat gctgaatgat tatctcgttg cttacgcaac caaatatcta atgtatgtcc
     2641 ctctgcatca atggcacgat ataaatagct ccattttcct tttattttga tgtacgtctc
     2701 atcaatacgc catttgtaat aagctttttt atgctttttc ttccaaattt gatataaaat
     2761 tggggcatat tcttgaaccc aacggtagac cgttgaatga tgaacgttta caccacgtcc
     2821 ccttaat
//
