LOCUS       CP150829                2971 bp    DNA     circular BCT 24-APR-2024
DEFINITION  Staphylococcus aureus strain BSN66 plasmid pHD75, complete sequence.
ACCESSION   CP150829
VERSION     CP150829.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637652
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN66 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN66
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP053637, CP052947
            Genome Coverage                   :: 76.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/23/2024 11:18:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,898
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 47 of 91
            Pseudo Genes (incomplete)         :: 44 of 91
            Pseudo Genes (internal stop)      :: 18 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN66"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pHD75"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-04-20"
                     /collected_by="Andrew D. Berti"
     gene            complement(828..1751)
                     /locus_tag="UXR62_00005"
     CDS             complement(828..1751)
                     /locus_tag="UXR62_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WYZ05982.2"
                     /translation="MDKYTEKKQRNQVFQKFIKRHVKEGQMDLIKECNTFLSFVADRTL
                     EKQKLHKSNLCKNRFCPVCSWRKARKDALGLSLMMQYIKQKEDKQFIFLTLTTPNVTVE
                     HLEDEIKNYNESFRRLSNRKHFKSIAKGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYF
                     TDKRYYISQKEWLNLWRDVTGIDEITQVHVQKIKQNNNKELYEMAKYSGKDSDYLVNQK
                     VFDTFYKSLKGKQILVYSGLFKEARKKLKNGDLDYLKEVDPTEYIYQIFYHWNQKEYLA
                     SEIFDLTEEEKSRINHQMIDEIDEEK"
                     /name="protein rep"
     gene            2116..2439
                     /gene="qacC"
                     /locus_tag="UXR62_00010"
     ARG             2117..2436
                     /name="qacC"
                     /gene="qacC"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2681..2869)
                     /locus_tag="UXR62_00015"
     CDS             complement(2681..2869)
                     /locus_tag="UXR62_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WYZ05980.2"
                     /translation="MTALKIILMLCISAMVGDFVLVIFESMPINIWVARFIGGVGASLS
                     ALLFAYLFIFKDKKQRQ"
                     /name="hypothetical protein"
     ARG             2117..2439
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="71.96"
                     /coverage="100.0"
     Typing          complement(829..1751)
                     /name="rep_cluster_1281"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.675"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatggtcggc ttaatagctc acgctatgcc gacattcgtc tgcaagttta gttaagggtt
       61 cttctcaaca tcaataaatt ttctcggcat aaatgcgtgt tctactcccc gaatttttat
      121 tcaaacaaca tgaaaatttg ataaatagct agtaccagca acacaacata tacatactta
      181 ttcgttaaaa aattctctaa tgcttctcta aatttcattt tttaacaact cctgttttct
      241 cataaattta tccactttca gtttttcttt taacaaactt tatcataact cttacaaaaa
      301 ttttgagtgc ctttattttt aattttaaag gggtattttg aagaaacact aaatatttac
      361 atagttttat atccaaaatt ctatataagc gcttaaaacg caaatgagag ccaaataaat
      421 atattttgat ttcacatcac aaatttttag caaaaccagt tgcgaaattt ttagacactg
      481 cccatctaca tgcaaactaa aattttgcat aaaatggctc aaaaaatcat caactttttt
      541 agttcctgca ggggcaattt ctttcaaaaa aacacggaat tttgaattgg ctctaaattc
      601 tttgtaagag cataagggaa atttgcatac atttaaaaat gtatggtctg gcgaagccag
      661 acatttcaag ggatacagcg attttaaaaa ggtatgcatt ttttgcgttt tgtacccagg
      721 ttgtacccct atatattgtg tctgtaccca gtttgtaccc agccattttt aacgtatttt
      781 caacaaaaaa acgctagctt tttgctaacg tttaagaaaa tttattttta tttttcttcg
      841 tcaatttcat caatcatttg atgattaatt ctactttttt cttcttcagt caaatcaaaa
      901 atttcactcg ctaagtattc tttttggttc cagtgataaa aaatttgata aatatattct
      961 gtcggatcta cttctttaag ataatctaaa tctccatttt ttaatttctt tcttgcctct
     1021 ttaaacagtc cagaataaac aagaatttgt tttcccttaa gagatttata aaatgtatcg
     1081 aacacttttt gattaactaa ataatcgcta tctttaccag aatacttcgc catttcgtat
     1141 aattctttgt tgttgttctg tttgattttt tgaacatgta cttgtgtgat ttcatcaatt
     1201 ccagtcacat ctcgccataa attcagccat tctttttgac taatataata tcgtttgtct
     1261 gtaaaatacg atttgttaac agcaatcaaa acatgaaaat gtggattata atcatcgcgt
     1321 tttttgttgt aagtaatttc taattttctt acgtaacctt tagctataga tttaaaatgt
     1381 ttacgattac ttaatcgtct aaacgactca ttataatttt ttatttcatc ttccaaatgc
     1441 tcaactgtta catttggtgt cgtaagtgtt aaaaagatga attgtttatc ttctttttgc
     1501 ttaatatatt gcatcatcaa tgataaacct aacgcatctt ttctcgcttt tcgccatgaa
     1561 catacaggac aaaatcgatt tttacacaaa ttagatttat gcaatttctg tttctctaac
     1621 gttctatctg ccacaaaact caagaatgta ttgcactcct ttatcaaatc catttgccct
     1681 tctttgacat gtcgcttaat aaatttctga aatacttgat ttctttgttt cttctcagta
     1741 tacttatcca tgttataaca caacaaaaca acttagtttt acacaaacta atgccataaa
     1801 aaaagttgct ttttctcctt tcttgtaaag ttttggcttg gacaccataa actctactat
     1861 actaggtatg aaaaagcaac tttttttatg ctcgaaacca gatataccaa cagattaagg
     1921 gtaactatta tagcttgtaa tagttaccta tagtatcaag acaagaagaa actcgttttc
     1981 aactcgtttc aaaaaccttt caaaaaccat caatccacaa aaataccacg cgaatgacac
     2041 tcaaaataca agactacaat taaaaaatac ttagaataaa attaaataaa atacgaaaat
     2101 taaaaggagt taaaaatgcc ttatatttat ttaataatag ccataagtac tgaagttatt
     2161 ggaagtgcat ttcttaaatc ttcagaaggc ttttcaaaat ttataccatc cttaggaaca
     2221 ataatttcat ttggaatttg tttctatttt ttaagtaaaa caatgcaaca cctaccacta
     2281 aatataactt atgcaacttg ggcgggacta ggtttagtct taacaaccgt agtctcaata
     2341 attattttca aagaacaaat aaatctaata actatagtat ctatagtttt aatcatagtc
     2401 ggcgtagttt cgttaaacat tttcggaaca tcgcattaat tgctttattc caattgcttt
     2461 attgacgttg agcctcggaa cccttaacaa tcccaaaact tgtcgaatgg tcggcttaat
     2521 agctcacgct atgccgacat tcgtctgcaa gtttagttaa gggttcttct caacgcacaa
     2581 taaattttct cggcataaat gcctgctcta ctttttgttc ttactcactt tgatagccaa
     2641 aaatgccatt ccaatgttag caacactgaa catatttgtt ttattgacgt tgctttttat
     2701 ccttaaaaat aaataagtag gcaaacaata aagctgataa agaagctcct actcctccga
     2761 taaatctagc aacccagata ttaatcggca tactttcaaa aatcactaac acaaaatctc
     2821 cgaccatagc agaaatacat aacatcaaga taatttttag agcagtcata aaatcactcc
     2881 ttttttctta acactactaa aattatcgct ttattccaat tgctttattg acgttgagcc
     2941 tcggaaccct taacaatccc aaaacttgtt g
//
