LOCUS       CP150110                2474 bp    DNA     circular BCT 02-APR-2024
DEFINITION  Staphylococcus aureus strain BSN211 plasmid pCN1, complete sequence.
ACCESSION   CP150110
VERSION     CP150110.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637791
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN211 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN211
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP043386, CP014443, CP014442, CP003981
            Genome Coverage                   :: 128.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/27/2024 14:00:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,005
            CDSs (total)                      :: 2,923
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 48 of 89
            Pseudo Genes (incomplete)         :: 46 of 89
            Pseudo Genes (internal stop)      :: 16 of 89
            Pseudo Genes (multiple problems)  :: 20 of 89
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN211"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pCN1"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2018-05-19"
                     /collected_by="Andrew D. Berti"
     gene            84..818
                     /gene="erm(C)"
                     /locus_tag="UXR80_14840"
     ARG             85..815
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1267..1743
                     /locus_tag="UXR80_14845"
     CDS             1267..1743
                     /locus_tag="UXR80_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WYL34607.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             85..818
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.18"
                     /coverage="100.0"
     Typing          1268..1753
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatcaacc aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatta
       61 taaccaaatt aaagagggtt ataatgaacg agaaaaatat aaaacacagt caaaacttta
      121 ttacttcaaa acataatata gataaaataa tgacaaatat aagattaaat gaacatgata
      181 atatctttga aatcggctca ggaaaaggcc attttaccct tgaattagta cagaggtgta
      241 atttcgtaac tgccattgaa atagaccata aattatgcaa aactacagaa aataaacttg
      301 ttgatcacga taatttccaa gttttaaaca aggatatatt gcagtttaaa tttcctaaaa
      361 accaatccta taaaatattt ggtaatatac cttataacat aagtacagat ataatacgca
      421 aaattgtttt tgatagtata gctgatgaga tttatttaat cgtggaatac gggtttgcta
      481 aaagattatt aaatacaaaa cgctcattgg cattactttt aatggcagaa gttgatattt
      541 ctatattaag tatggttcca agagaatatt ttcatcctaa acctaaagtg aatagctcac
      601 ttatcagatt aaatagaaaa aaatcaagaa tatcacacaa agataaacag aagtataatt
      661 atttcgttat gaaatgggtt aacaaagaat acaagaaaat atttacaaaa aatcaattta
      721 acaattcctt aaaacatgca ggaattgacg atttaaacaa tattagcttt gaacaattct
      781 tatctctttt caatagctat aaattattta ataagtaagt taagggatgc ataaactgca
      841 tcccttaact tgtttttcgt gtacctattt tttgtgaatc gattatgtct tttgcgcatt
      901 cacttctttt ctatataaat atgagcgaag cgaataagcg tcggaaaagc agcaaaaagt
      961 ttcctttttg ctgttggagc atgggggttc agggggtgca gtatctgacg tcaatgccga
     1021 gcgaaagcga gccgaagggt agcatttacg ttagataacc ccctgatatg ctccgacgct
     1081 ttatatagaa aagaagattc aactaggtaa aatcttaata taggttgaga tgataaggtt
     1141 tataaggaat ttgtttgttc taatttttca ctcattttgt tctaatttct tttaacaaat
     1201 gttctttttt tttttagaac agttatgata tagttagaat agtttaaaat aaggagtgag
     1261 aaaaagatga aagaaagata tggaacagtc tataaaggct ctcagaggct catagacgaa
     1321 gaaagtggag aagtcataga ggtagacaag ttataccgta aacaaacgtc tggtaacttc
     1381 gtaaaggcat atatagtgca attaataagt atgttagata tgattggcgg aaaaaaactt
     1441 aaaatcgtta actatatcct agataatgtc cacttaagta acaatacaat gatagctaca
     1501 acaagagaaa tagcaaaagc tacaggaaca agtctacaaa cagtaataac aacacttaaa
     1561 atcttagaag aaggaaatat tataaaaaga aaaactggag tattaatgtt aaaccctgaa
     1621 ctactaatga gaggcgacga ccaaaaacaa aaatacctct tactcgaatt tgggaacttt
     1681 gagcaagagg caaatgaaaa acaagaaaat gcattatctg attattattc tttcaaggac
     1741 tagtataaca taaaatcgtc tacaaataga caaaaaacct gcacgcttaa tgtagatcaa
     1801 aagcttaacg caaatgaaat agattgacct cccaataaca ccacgtagtt attgggaggt
     1861 caatctatga aatgcgatta agctttttct aattcgcata agcgtgcagg tttaaagtac
     1921 ataaaaaata taatgaaaaa aagcatcatt atactaacgt tataccaaca ttatactctc
     1981 attatactaa ttgcttattc caatttccta ttggttggaa ccaacaggcg ttagtgtgtt
     2041 gttgagttgg tactttcatg ggattaatcc catgaaaccc ccaaccaact cgccaaagct
     2101 ttggctaaca cacacgccat tccaaccaat agttttctcg gcataaagcc atgctctgac
     2161 gcttaagtgc actaatgcct ttaaaaaaca ttaaagtcta acacactaga cttatttact
     2221 tcgtaattaa gtcgttaaac cgtgtgctct acgaccaaaa ctataaaacc tttaagaact
     2281 ttcttttttc ttgtaaaaaa agaaactaga taaatctctc atatctttta ttcaataatc
     2341 gcatcagatt gcagtataaa tttaacgatc actcatcatg ttcatattta tcagagctcg
     2401 tgctataatt atactaattt tataaggagg aaaaaatatg gacattttta gtatttttgt
     2461 aatcagcaca gtta
//
