LOCUS       CP150105                3287 bp    DNA     circular BCT 02-APR-2024
DEFINITION  Staphylococcus aureus strain BSN209 plasmid pUSA01, complete
            sequence.
ACCESSION   CP150105
VERSION     CP150105.1
DBLINK      BioProject: PRJNA1048360
            BioSample: SAMN38637789
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN209 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN209
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP014445, CP014385
            Genome Coverage                   :: 136.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/27/2024 13:30:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,913
            CDSs (total)                      :: 2,831
            Genes (coding)                    :: 2,747
            CDSs (with protein)               :: 2,747
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 14 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University College of Pharmacy, Detroit, MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN209"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pUSA01"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2018-04-28"
                     /collected_by="Andrew D. Berti"
     gene            1..996
                     /locus_tag="UXQ91_14390"
     CDS             1..996
                     /locus_tag="UXQ91_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WYL43094.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1741..1929
                     /locus_tag="UXQ91_14395"
     CDS             1741..1929
                     /locus_tag="UXQ91_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WYL43092.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2255..2836)
                     /locus_tag="UXQ91_14400"
     CDS             complement(2255..2836)
                     /locus_tag="UXQ91_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WYL43093.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1229..1393
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          2..996
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacacaa tatattgcgt ttgtcttatt
     1081 tttgtgacac tacatacagc atttgtcaca aaaataagac agtacttttt tataaaaaat
     1141 caacaaaaaa gacattagtg ttgactaatg tcttttttgt tgatttttta taaaaaagta
     1201 ctgtcttatt tttgtgacaa atgctgtatg tagtgtcaca aaaataagac aaacgcaata
     1261 tattgtgtca caaaaataag acagtacagc tttgtatgat atcactttaa aataccttaa
     1321 aacccctgga atttctggct ttgccagacc tatcattttt gaatgatagc aaattctcct
     1381 tatgctctta cggagttttt agagaaaaat taaaaattct cgatttttga taaaaaacgc
     1441 cctgcaggaa tttagaaaaa catgtggaag ttttaaagga ttttatgcta atttttaatt
     1501 tgcatgtaaa tgggcagtgt taaaaaatta gtcactggtt ttgctgaaat ttttgtttgt
     1561 gaaattacca tatatttatt tggctcatat ttgcgtttta agagcttgta tatattttta
     1621 agttaaaaac ctatatgata ccccctaaac ctttaaaata cccccttaaa actcaaaata
     1681 aaggtattta taatttgagt aagtcttgtg ataaagtttt ttaaaaaagg agtgtttatt
     1741 ttgaataaaa acacagtgtt agatgaaggt tacgttatag caactatttt aaatgttttc
     1801 tttttacttg gattaatttt tatatctcat ttggaaaatt tatatatatt aattccgtat
     1861 gttattttga tgggaataaa tgctatttat ttggttgtta aggttatgaa tttcaaaaag
     1921 aataattaga atatttgagg ggattttgaa acgagtttct tcttgttttc acactgtttt
     1981 tttattccta ttggtgttgt tgcttacttt ttgtttttct ataaagatga tgcttttgac
     2041 gagatggaag aaaaatatga ttatcatgaa gataataaaa aatagacgac gcatttatgc
     2101 cgagaaaatt tattgatgtt gagaagaacc cttaactaaa cttgcagacg aatgtcggca
     2161 tagcgtgagc tattaagccg accattcgac aagttttggg attgttaagg gttccgaggc
     2221 tcaacgtcaa taaagcaatt ggaataaata gtatctaaag taacccgtat ttaagattac
     2281 attgtacatc cttcgaaacc atagtagctg cgattccagc taggtttcct tttatcccaa
     2341 ttttagctaa tttttttgct acgcccttta agtttttggc tttaacatct tcaaaaactg
     2401 ctgtgattat gtttccagtt aaaagttctc catatgagtt taaaacttca gaatagaaac
     2461 agtcaccgct atttttgtag ttcccaccaa ctgctctagg tgatattgaa ctagttttct
     2521 ttttattctc tatgtcgttt tttagttttt ggaatccttc aggaacatat ccatatcgag
     2581 atttggcatt ttctagatta acatctttag ctgttccgtc tttatctaaa ataataattt
     2641 tgccatatat gtattttagt tctttttcaa tttctttttc gtttaaagtt tcaacgtttt
     2701 tatctgcaat gttatttttg ttttgggctt gagcttcttt gaaattaaaa tccaagacca
     2761 ttgaaagtat ggttaaaata cttaaagata ctactaaaat ctttcttagt gatatactgt
     2821 tttgaattgc attcattgta aatgccccct tgttttgtta tttaaatgaa ttcaatataa
     2881 tattaacata taaaaacaaa ggagtttaat atgaataagt ttttttactt tttgattatt
     2941 ttattatctg tatttttaat ttttaatttt gttaattcga ttttatttga taatcctttt
     3001 gactttttag aaagttttat ttattcattg gttttcacat taatttattt aggtttagag
     3061 ggtcataaaa agaagaagga ataggttgtt ttttgaaacg agtgtgaacg agtttcttct
     3121 tgtcttgata ctatatagaa ataactcgat tttatatata tagctgtaac tgttgatatt
     3181 acagtgttta aacgtgtttt tgtgcgtgaa aggaaaattt gacaataaaa aaccccagtt
     3241 atattattaa ggtgtcgaat cttaaataat actgggggtc tttttat
//
