LOCUS       CP148999                5596 bp    DNA     circular BCT 26-MAR-2024
DEFINITION  Klebsiella pneumoniae strain 130125 plasmid p2_130125, complete
            sequence.
ACCESSION   CP148999
VERSION     CP148999.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN40534517
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 130125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2024) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2024 10:29:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,509
            Genes (coding)                    :: 5,379
            CDSs (with protein)               :: 5,379
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 50 of 130
            Pseudo Genes (incomplete)         :: 82 of 130
            Pseudo Genes (internal stop)      :: 14 of 130
            Pseudo Genes (multiple problems)  :: 16 of 130
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130125"
                     /isolation_source="respiratory tract secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_130125"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-07-03"
     gene            complement(join(5490..5596,1..52))
                     /locus_tag="WIS46_28110"
     CDS             complement(join(5490..5596,1..52))
                     /locus_tag="WIS46_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WXU16636.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            692..868
                     /locus_tag="WIS46_28115"
     CDS             692..868
                     /locus_tag="WIS46_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16637.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            858..1202
                     /locus_tag="WIS46_28120"
     CDS             858..1202
                     /locus_tag="WIS46_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WXU16638.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1411..1845
                     /locus_tag="WIS46_28125"
                     /pseudo=""
     CDS             1411..1845
                     /locus_tag="WIS46_28125"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1880..2389
                     /locus_tag="WIS46_28130"
     CDS             1880..2389
                     /locus_tag="WIS46_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WXU16639.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2396..2608
                     /locus_tag="WIS46_28135"
     CDS             2396..2608
                     /locus_tag="WIS46_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WXU16640.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3275..3499
                     /locus_tag="WIS46_28140"
     CDS             3275..3499
                     /locus_tag="WIS46_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WXU16631.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3483..3749
                     /locus_tag="WIS46_28145"
     CDS             3483..3749
                     /locus_tag="WIS46_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WXU16632.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3819..4169)
                     /locus_tag="WIS46_28150"
     CDS             complement(3819..4169)
                     /locus_tag="WIS46_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16633.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4235..4780)
                     /locus_tag="WIS46_28155"
     CDS             complement(4235..4780)
                     /locus_tag="WIS46_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16634.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4806..5132)
                     /locus_tag="WIS46_28160"
     CDS             complement(4806..5132)
                     /locus_tag="WIS46_28160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16635.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          527..606
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2..332)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.82"
                     /coverage="81.0"
                     /evalue="2.28e-115"
     Typing          1193..2724
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatagttacc ggaaagggcg cagcagtcgg gctgaacggg gggttcgtgc atacagccca
       61 gcttggagcg aacgacctaa accgagccga gataccagca gcgtgagcta tgagaaagcg
      121 ccacgcttcc cgaaagggaa aaaggcggaa caggtatccg gtaaacggca gggtcggaac
      181 aggagagcgc acgagggagc cgccggagag aaatcaccgg catctttaag tcctgtcggg
      241 tttcgtcctc ctctgacttg agcgtccatt tctgtgatgc tcgtcagggg aggcggagcc
      301 tatggaaaaa cggcaacgcc gcatcctttt ctccggccct ttctgatttg agcgcctttg
      361 ctttatcccg ttatccgtga gtcggtcacg cccgccgcag ccgaacggca ggagcgcagc
      421 taccgagtga gcgaggaggc gtcatttcat ctgtccgtgc atggtattgc ggacgctcct
      481 gagccacata tcacacctgc cacatgaggc actttcatga atcgtgctct catgccaaca
      541 tagtaagcca gtatacacag cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc
      601 cgctaacacc actgacgcgc tggcgcttgt ccaacgccgg agcgacccgg aaaacttttc
      661 cgtgccgtcc ggcgtttgcg aaggagggat aatgcagaag ccgaagaaga agccgctcgt
      721 cgtaaaaaaa agatcctggc atccggcagt gatggcaaat ctgatgcgtc aggcacagga
      781 ggaagattac cgccgggagc agatggcaga gcttgagcgt tcagcccggt cacattttgc
      841 ggatatcgga ggagaggatg gcggttaagc gtacccgttt tttaggtatc cgggtgaccg
      901 atggggagta ccagcagctg ctggagcgct gcaatggaag gcaactggcg gtgtggatgc
      961 gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg
     1021 ttttgctgcg ccagcttgcc gggatgggga acaacctcaa ccagatcgcc cggaaaatta
     1081 acggcggcca gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg atggccattg
     1141 atgccgaact cgagcggctg cggcatgccg tgctggaaaa gggggcagac gatgatcgtt
     1201 aagtttcatc cgcgagggcg aggcggcggt gccgggccgg tggattatct gctggggaaa
     1261 gaccgccagc gcgacggggc catcgtcctg cagggaaagc cggaggaggt ccgggagctt
     1321 atcgacgcct cgccctacgc caaaaagtac acctccggcg tcctgtcttt tgctgaacag
     1381 gatttaccgc ccggccagcg tgaaaagctg atggcgagct tcgagcgggt tctgatgccc
     1441 ggactcgata aagaccagta cagcgtgctg tgggttgaac accgggacaa ggggcggctg
     1501 gaactgaatt ttctggtacc gaacacggaa ctgctgaccg gcaggcgtct ccagccgtac
     1561 tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga ccgtggtgaa cgggcgactg
     1621 gggctgcacg acccgaacgc accggagaac cggcgcgcgc tggtgacgcc atcttcgtta
     1681 cccaaaacga agcaggaagc cgcagaggct attacgcggg gcttactggc cctcgcctcg
     1741 tcaggggagc ttaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc aggttttgag
     1801 gtcgtgcgca ccacaaagag cagcatcagc attgccgacc cggacggagg gcgaaacatc
     1861 cgacttaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact tagagcagaa
     1921 atcgaaagcg cagcagcaga gtaccggcga aatgctgaaa gccgcattca gcgagcacga
     1981 gaagtctgtc agagcggaac tgagcgaaag cgggaagaga atcagcgccg ccatccgcgc
     2041 ccacgagcag ggtatgagcg cggccatgca gtcgaaccgc ctgagcgtga tgcgtatggt
     2101 cgggcggaca tggctgacca tcacgatggt gtcaggactg ctgttcgcca gcctgagcgg
     2161 ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg gccgaaatcg accggcagaa
     2221 cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg acgtacctgg aagacagcaa
     2281 cggacgtttt ctggtgctgc cgaaggggac ggccgtagac aggacgcaga gctggacggt
     2341 cgggaacggg cggtcgaagc agaacgcgct gagactggtg aaggagtaag tgccgatgac
     2401 cgagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
     2461 gctggacgag tgggagagcg ccttcgggga atggcggaaa atgtcagggc ttatgcaacg
     2521 ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt cagcaggtgg aacgcttgag
     2581 cgggcaactg cagcgtttga gccggtagtg cagcgtcatg agatggccgt ggccgcagaa
     2641 cgggcgcacg agcaacggca gcacgaaaag gaactgacgg aagcgcgttc ccggcagcga
     2701 agctatgacg gtccgtcgct gggctgaaat gcaggaaaac agggttctca tcgggcattt
     2761 acgctgaaac cggatgtcat tcacctggct gcatggctat gcagccaggt aaaatttatc
     2821 tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg ttttacctga ctgccacccc
     2881 gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt gttatgttta atcagcgtga
     2941 acggcagata caaaaaagcc ccggagggct tttaactact gacttatttt tttgaagatt
     3001 tgctgggttt caatttgtca gtatttcccg gcgttttttt attgtcacgc tggcgtttat
     3061 caggttcccc tgttgaatcg gcaattcgtt tcggacccgg cttaagaccc ataatcgtta
     3121 tcctttcaat agttaagagg aaagccctca agctagatat attgaccgcg ttacggatta
     3181 tcaagagggg ctaacaagat tattttttgt tcttttaggt tgattgtggt actgttgata
     3241 tacatgtata gcaaatggtg ttcgggaggt ttatatgctt gctatccggt tatctgatga
     3301 gattgagtcc cgtctggact cgctggcgaa gcaaaccggc agaacaaaga cgttttatgc
     3361 gcgggaagca atactggcgc atctggaaga cctggaggat tattatcttt cagcagaaac
     3421 tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg tctgaagacg tgaggaagtc
     3481 acttggtctg gacgattaac tattccgatc gggcgctcaa atcgttacgc aagatggaca
     3541 aacagaacgc acgacggatt gtggatttta tgagtttacg cattgcagtt gctgccgatc
     3601 ctcgccagtc agggaagccg ctcaaaggtg agctgggcga gttctggcgc tatcgggtgg
     3661 gagattatcg cgttctgtgt gagatccgag atgacgagct tgttatcctt gccgccacga
     3721 ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg cgggcttttt tgatgccgac
     3781 aacaggtatt atggtaaata ctcgactaac tacgtgttct actgtttgtc ggttttagtt
     3841 aaccagcagg ttgtcttaac gataatgtag ccgttatttt taactaataa ggtcatattg
     3901 ttaacgtcag gcataataaa agacattatg gcctcagagt tatcatcaga agcagctctc
     3961 tgcattacta cttcgattcc cttttctttg aatctataag tatccagttg cctattaaac
     4021 ttagaaagac ctacaaccat acttagtttg ttatctttaa cagtaagaga tgcttcctga
     4081 gtaccattac atcggtagta agcggtgtaa ccgtcagcaa aagatcgacc ggggaatata
     4141 aatgctagga acgcaattaa ccaatacata gttgagaatc ttggtattac acatttcact
     4201 atcctctctt tcatttttcg ttcaccatac tttgttatct gatttttaat ttttctattt
     4261 tataaaccaa gtctctacca aaaaggcgaa aattatcctc aaataataat aattgtttaa
     4321 gaacatccat gaaagcagct gcttcaccct cagttatggg agctactcta tataacatat
     4381 aacctgagtt aagggtttct tttatacttt cttcaatgct cattataaat attagtggat
     4441 tatcattttt cttcccccat gggtttctaa ttaatccttc gatgtactca ggtcctttgc
     4501 gtcctattgc ctgcgcaata ttttttattt catctgcgtg agggctttgt tgtacagctt
     4561 tgtttatgcc tgcttgagtg attttcagag actctacacc gtgtatgttg tatgagataa
     4621 aatgtaataa attaagatgc aatgcaatta atccattttt gtttacatct gagtatttgg
     4681 ataaaaaacg taatgaagtg ttaactctcc cttgaagcct aacaattggg agtgctgcct
     4741 tgtatatttc cttctccgtt aaataccgtt taaaaaacat aattctccgc ctccgtatag
     4801 cgtgcctatt ttatttcgtc gtaaaactta gctctgaaat attcctttag aaccatcatg
     4861 tcattaataa ttgaatctat ctcatccatt ttttcttggt attcttttaa tggtgaaggg
     4921 agcggtaact caatatttga aaagtgattg gcgagatagg gatcaaggtt tctcatccat
     4981 tcaggctttt cgccaatgcg ttcttcaact gaaagtgaac ctgataatct ctggttaata
     5041 agatcactta gactcatttt ggttttattc gaaagccgtt gtaaggattg ctttaatcct
     5101 atatctagca tgatatttgt tctttctctc attacccccc cttgctattg cgttttttgt
     5161 tgcgcacaaa gctaccattg cgcattaaga aacgcaataa aaaaggaggg agggagtggt
     5221 ttgtacatga tgtgcagtgc gctgagggca tcagtggcgg gtgtttttcc gtgggatgga
     5281 tggagctccg gggataacag aactgacgat catttaaagg atcttcttga gatccttttt
     5341 ttctgcgcgt aatctcttgc actgtaaacg aaaaaaccgc ctggggaggc ggtttgatcg
     5401 aaggttaagt cagttgggga actgcttaac cgggtaactg ggtatacgag accgcagata
     5461 ccaaaactgt tctttcagtg tagccgcagt taggcaccca cttcaagacc tctcaatatc
     5521 tctctcgtat atatccagtt accaatggct gctgccagtg gcgtttttgc gtgtccgtcc
     5581 gggttggact caagat
//
