LOCUS       CP148998               10060 bp    DNA     circular BCT 26-MAR-2024
DEFINITION  Klebsiella pneumoniae strain 130125 plasmid p1_130125, complete
            sequence.
ACCESSION   CP148998
VERSION     CP148998.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN40534517
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 130125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2024) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2024 10:29:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,509
            Genes (coding)                    :: 5,379
            CDSs (with protein)               :: 5,379
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 50 of 130
            Pseudo Genes (incomplete)         :: 82 of 130
            Pseudo Genes (internal stop)      :: 14 of 130
            Pseudo Genes (multiple problems)  :: 16 of 130
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130125"
                     /isolation_source="respiratory tract secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_130125"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-07-03"
     gene            complement(join(9695..10060,1..1599))
                     /locus_tag="WIS46_28045"
     CDS             complement(join(9695..10060,1..1599))
                     /locus_tag="WIS46_28045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WXU16627.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3083..3232)
                     /locus_tag="WIS46_28050"
     CDS             complement(3083..3232)
                     /locus_tag="WIS46_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WXU16628.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3317..3574)
                     /locus_tag="WIS46_28055"
     CDS             complement(3317..3574)
                     /locus_tag="WIS46_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WXU16629.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3584..5269)
                     /locus_tag="WIS46_28060"
     CDS             complement(3584..5269)
                     /locus_tag="WIS46_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WXU16630.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5596..5841
                     /locus_tag="WIS46_28065"
     CDS             5596..5841
                     /locus_tag="WIS46_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16618.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6115..6486
                     /gene="tolA"
                     /locus_tag="WIS46_28070"
     CDS             6115..6486
                     /gene="tolA"
                     /locus_tag="WIS46_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WXU16619.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6483..6848
                     /locus_tag="WIS46_28075"
     CDS             6483..6848
                     /locus_tag="WIS46_28075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WXU16620.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7023..7301
                     /locus_tag="WIS46_28080"
     CDS             7023..7301
                     /locus_tag="WIS46_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WXU16621.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7289..7603
                     /locus_tag="WIS46_28085"
     CDS             7289..7603
                     /locus_tag="WIS46_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WXU16622.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7767..8195
                     /locus_tag="WIS46_28090"
     CDS             7767..8195
                     /locus_tag="WIS46_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16623.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8221..8400
                     /locus_tag="WIS46_28095"
     CDS             8221..8400
                     /locus_tag="WIS46_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WXU16624.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8427..8957)
                     /locus_tag="WIS46_28100"
     CDS             complement(8427..8957)
                     /locus_tag="WIS46_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXU16625.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8964..9695)
                     /gene="mobC"
                     /locus_tag="WIS46_28105"
     CDS             complement(8964..9695)
                     /gene="mobC"
                     /locus_tag="WIS46_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WXU16626.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2402..2980
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8968..9695)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cgcgcgatag aaccagcgga tcggcgtgtt atccagtacc gtggttttca caaaatcagc
       61 gggcagatcc tgcccgcact gaccgaagtc gcccagcgac tggtgcgcga gcagcaggtt
      121 gcagtcccgg ctgcgcagcg tccccagcgc gttcagcacg tacttcgaca gcagatattt
      181 aatctcatcg agcatgatgc tcgcgtgcgg ccacctccgg aactcatccc gcgcaatgat
      241 gatttgcgcg cagcgggcaa acagcatctt ctgcacccgg atcaccgcct catcgtccat
      301 cgagccgatg acgtacaggc agccgccgcc gttgattatc cccgccacat cgataccgct
      361 gtccgtctgc aggacgctca ggtcggccac gttctccagc ttggtgatca gccccttcac
      421 gtcctttttc agggcctccg gcaacgcgta cgccgccgcc agcacctgct gaatgtttgc
      481 cccctgcggg aactgctccg caatcagctt cgccgctttc tgctcgctga tgcggtagtg
      541 gtccgccacg tcgccggtct cactcaggtt aaacccggca accagcagat tcttcagggc
      601 gtactggtcg agatcgcgga acagattcag ctgcgccggt ttccccggcc gcagatccag
      661 cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc gcccacgcgt cccctttggg
      721 gtcgaagtac accacagcgt ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc
      781 catcgcgctc ttgccgctcc cgggcaggcc gaggatctga atattggtct tgcgccaggt
      841 atcacgaggg accagcaccg gctggccctg ctcatcctta cccagaaaca ccgcctgctg
      901 tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta accggtaccg gctccccgaa
      961 atgcaggtgg cggacgtctg tcatttctcc ggtccgctgg ctgctgcgcc ggctctcctg
     1021 attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa tgaccatcaa gcagcaggaa
     1081 cgccagcagc cgcgccagaa ggacgccggc gacaagccct ccggcacacc aggctatccc
     1141 gaacaggtta gccggctgca gcaggtaata atctgcgttc gcatagaggt ccgggcgcag
     1201 gacggtttct ccccagctct cgataaacac ggcgacaacc agccccccca gaagggccag
     1261 accggcagcg ccggtcagcc agtacgcgta gttccagaca tgtcgccgtg ccctgaccat
     1321 ccgcccgggt gaatccagcg aggccagcac cgaaaatggc acagtccggt tattatcgcg
     1381 cttatagcga tcggcatact cccgccgcgc cttatggctc agccccacaa aacgcattcg
     1441 gccggcccag agttcagcca gaccgaaaaa caggccgata accagaaaaa aaccggccag
     1501 actgtaatac tcccggtaca gccagctgac ccacgggtaa atctgccggg ccagctccgg
     1561 gatatgccac tgccaggcaa gcgaatccgt attaaacatg caatctctct ccttcagacg
     1621 acgggtttac cggcgtgatg cgcagccccc gctgcacaag ccgcggacat gccccaaggc
     1681 atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct ttcaccgcgg aggccagcct
     1741 ctttccgccc catgcggaaa aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg
     1801 tgagaggaaa aggcggcacc ccagtgcggt cttttccggt gttccggccc ccgtcagagt
     1861 tcgccatagc gagcggctga ccacccgcaa aatcaggagc agctttgctg cttcgtcagg
     1921 tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg
     1981 aatttctggc gtggccggcg tccagccgga cagagccaga aagaggggaa aagaccgtgc
     2041 gtcggtggcg tccagccagc gtaagcaagg tcttttcccc tgaagtcagc cacgccccag
     2101 cggggatgac ttgcatttct ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa
     2161 atgttcagcg aaaaaccgac atggttcacc tatcctgata attgatcgtc agggaataga
     2221 aagacttaat caggggacaa tatcccacat cagcgcgtcc ggtgaccgtc ttgcaggtgc
     2281 cggggaaatt gcgcctcctc gctcactcgg tcgctacgct ccgggcgtga gactgcggcg
     2341 ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg ggactaacat
     2401 gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc
     2461 agagttcaca taaacagacg ctttttcggt gcatctgtgg gagccgtgag gctcaaccat
     2521 gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggtagg
     2581 tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc tgtccgccgt
     2641 tctcccttgc gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt
     2701 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
     2761 cgccttatcc ggtaactgtt cacttgagtc caacccggga aagcacgcaa aacgccactg
     2821 gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc
     2881 tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct
     2941 gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca
     3001 cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct
     3061 caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca gaggaggacg
     3121 atggtgccac cgttccgccc tgaacatccc ggatataatt tgcctgacat gcggccagaa
     3181 gaaagccagc aacaatcaat ataaaaagaa aaatagtctt tacctttttc atatactttc
     3241 cctgtattta aagactccgg cattcatccc tgaacaccgg aagtcagtat ttcaaatcat
     3301 ccttgataat tttttgttac cagttaccgt cccgataatc aaaagctata taacaattat
     3361 ccacatcagg ttttatttga tttttaaaaa atggctgtag taaaggaacc cattctttct
     3421 tcaaatcaaa ctcaccatta ttaatattat cctttaaagg cattcccaag ctttcaatga
     3481 cagaaccatc gtcaccaaag tctttagaat actcttcccc tttaaactct tcagttttct
     3541 tatcaaacca tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga tattttttaa
     3601 tgttccgttt tggatctggc cctttaacct gcttacccgt tttgggatca aatgcgccta
     3661 ggtgttcgcc atcactggca cgataccctt caagctcacc gtgctgggaa tcccattcat
     3721 aaattttacg ttttttatcg ccataccatc gggcccgctt accaccacca ccttgtttag
     3781 gtgttttagg atcacctcgc tttaactctc caagtccctt tatgtcttct gttttaggta
     3841 ctggatgata atcatggccg tagtctttag tccccttgcg aggcttgttc ttttcctcat
     3901 ttaacttatt ttctgcatcc ttcgcttttt tctccttttg tttccggctc tccatcgccc
     3961 cggtaagtgc agccttatct ctggataagg attcattcat tgcgtttaaa gatgcctgag
     4021 ccgcttttaa tttctcattc tgattctgaa tatccttatc aagatagcga acctgatcct
     4081 gccacccgga gccatattca tgaggcgggt tataagtcac aaaatcatct ttatttgcct
     4141 ctgcctcttt taccttttta tcggcttcct gaacagcagc ccttgcaccg gggagcgcct
     4201 tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt tttatcttcg gcatccagct
     4261 cagctttcgc tttatcatag tctcttttca gcccctcttc cggatgagcg gcgtcccatg
     4321 cctgctggcg acgcttttct tcctcctgac gctgttttac ctgtgccggg gtaagaacat
     4381 ctgtcaccga cacataaacc ggcttctgtc cggtctcttt cgggaaacga ataacggcct
     4441 cacgggagtt accaccggct gtaaaaccag ccggacgtga atcacctttt tcagcaacaa
     4501 tgccttttgg cggggctttc gctgtcggaa cacctttcgg tacgctcacc tgcagagacg
     4561 ggagacccgg aatcgacaca ctgaataccc ccggacgttt tgtcggtttc gcatccacca
     4621 cagggacact cattggccga cctgcgacaa ccgcaatatg ctgccgctca tccttcacca
     4681 catccacaac ccgttgtctg acacgaaccg tcgcctggtc cagtggtaaa gtacttgccg
     4741 gagtctccgt caccgcatcg gccggtaatg acgtcataat ttttgacatc attttcggat
     4801 catcttttgc aatctcagaa ggcagcacac cgtagatcgc aatcccccac agaccaaact
     4861 taaacggccc cttcagggca gccagcacat cagcaacggc ggccgacaac gcatcgccgg
     4921 aaactgataa agccagtcct tcagcccccg gagtagccag ggcaggcaga ccgaaggcca
     4981 tcgcagtggc cgaagactgt ccgccaccag agctaccgct atgattgccg ctgttaccac
     5041 cattaccacc gggtttactc ccgccgttac caaactcccc cggattatag ttatgaatat
     5101 ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc gtaccaccac
     5161 ctgatgaagg gccaccttta ccagacgtac cgttcacatt cccactggcc tgaccaccat
     5221 tatgtcccag acctgaatta cccggacctc gaccgtctcc accactcata ccgttttcct
     5281 ctttgacatt taaaacaata aattaaagcc aacatactgt acatatacac agtataatta
     5341 ttaccttact cctcccgttt tcaggtgtca atatatgaga ttagttctca tcttcaggca
     5401 caaaaaagcg cccggagcca cctgtaagca gatcgcgtct tttcccgaaa atttctcatg
     5461 gcaacaaatt ttattattta agacattcat cacacttaca tttcagatgg tatagacaca
     5521 tctgaaagct aactggcatg aggagaggac ctgcggttaa actgttcggg aaacctgaga
     5581 cgcccggagg ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa gcgcctggcg
     5641 gacgcctttg tcgctcaggc actggccatg ggcctgaaag ggaacaaaac gctgccaaag
     5701 gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga acgtgaagcc
     5761 aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcta cggaaaccac cttcacctgg
     5821 cagccacagc gccgccggta aggggtattt accataacgt cgcattgccc gcaccgctca
     5881 gacggtctga gggcgtttcc ggcggctacg ggataaaaca ggcaacaaac cacccggaaa
     5941 accgccggaa acgcgcccgg taaattgtaa cccgctgcat gcctatgcac gcaactgcat
     6001 ggcgggccgt ttggtgtaaa aacgatacga taagccgtgt ttatgtaaat gaacgtggcc
     6061 gtaaagagtg acaaatatac atgtaacatt ttttactgac aggtcatcaa cccgatgcgt
     6121 ttaataccct ttatcctgct ggcattgtgc agccccttac tcctgacggc ctgtaagcat
     6181 tccaccaccc agccgccgaa aatttatact ctggataagt catctaaaat cagtagctac
     6241 gctgcggcca tcaagaccgc tgttgaaatg cagatgtttg aaccagaggc ctatgcggga
     6301 aaaatgtgca ccgtgcggat atccatacaa cgtgatggca ctctgaacag cgccattgct
     6361 gaaggaggag atccgaaact ctgtaaagca gcaatatcag ccgttaccca cgccaaaatt
     6421 ccgccggcac cggatgaaaa tacctggcaa atattcaaaa acgcccctct ggatttcaga
     6481 ccatgaaagc ttttactctg acagtgctgg cggccatgtt cctcactccg gtacatgcga
     6541 taccactaaa acccgtaacc acactgcagc caacctaccc ggtacaggcc gcagcaaaac
     6601 gcatcgaagg tcaggtgacc gccacgtttg atattctgga cgatggacga gtggacaacg
     6661 tgtcgcttac cggctgtcac cttttttttc gggaaacgct gacggcaatg aggcactggc
     6721 tatatcccgc agggcacccg gcaatgcgac aggttataaa ttttgaattc agtctgaaaa
     6781 atgacggctt acaggatccg cgaccagtca ccacccaaaa gctcaatgat gatggttgcc
     6841 gccagtaaca ggtcctttaa cccgcaggct gcaatagtcc tggatgatga gtgagcctgt
     6901 agcagccctg attagtggaa gcgaagctga aaaatgggca gcaaaagccg tatttctggc
     6961 aaatagcgcc gccacggatt aaagtagtca tgtgtaatac atatgactac aaaggggctt
     7021 ttatgtcatc agcaacatcc atcaggctcg atgaagaact taaggaccgg ctgaaaacgc
     7081 tggccgatga ccggcaccgc tcagcgcacg cgctaatgct ggaggcaatc accgaataca
     7141 tcgaccggga agagaagcgc agccagtacc tgcgggacgg acaggccgca tggcagcact
     7201 atcaggaaac gggtctgcac cttactgcgg aagaagcgga agcctggata agtacctggg
     7261 gaactgagaa cgagcaggat gctccgccat gccacaggta acgatttccg cgctggcaca
     7321 gcgcgattta caacgccttc aggactttct caaaaccaaa aatcggctgg cagccagaaa
     7381 aggaggtgag gtgatcgtcc gggctatcca gcaactgaaa acattgccag acattggccg
     7441 cccggtgccg tttctgccgc tggaatataa ggaactggtg atcgggtttg gcgacagtgg
     7501 ctatgtgatg ctctaccgcc acgaccggga aatggaccag attgtgatcg tcaccgtcag
     7561 gcatcagaaa gaatccgggt atccgggggc agacagcctc taataaccga tgacgggggc
     7621 gcaattcatt gcgtggccgt cgccggtttg acaagcgcag cgcgtcagaa aaccacatcc
     7681 tgaacggaaa aacgggtaaa attcagcgat acatttaccg tgagaaatta cacacctttc
     7741 acacccgata aaatcagggg tatacagtgg cgaaaacatt gctcaacctt tcacaggcag
     7801 cacaagccgc gggcattacc agaagaacgc tatacaatca tgtaaaacag ggaaaggtta
     7861 ccgtgtcacg tgacgggaaa aacaaccctg tcgtggatgt gtcagaactg atccgtgtat
     7921 acggaaatgt gaatatccct gagaaacaaa ttcccggcat ttcacaccgt gaaaatacac
     7981 aaaaaaattt cccacaggaa cagcttctgg caatgcaaaa ggaactggcc gatctccggc
     8041 aggctgtcac cctgatgctg gaagataaaa catcccggga agaagaacgc cgccagcacg
     8101 atgatgagcg ccggaaactc caggccgaag tggacagatt gacaacagaa ctgacacaga
     8161 agaaaaaacg cttctggtcc ggctggttta gctgagaact taccgaaggg aatggagaac
     8221 atgacgaagc aggaaaaaac tgccctcaat atggctaaat tcattaagga tcagtcgctg
     8281 ctgttgctgg aaaagctgaa cgagctggac atggataccc cggccgacat gtgcgaacgg
     8341 cttcacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg ggaagaataa
     8401 cagcaccggc catgccggtg ctggcgttat tttttgccga acggccacca ccggcgggcg
     8461 ggtttgtcct ccggcagttc aggcaccggc acgttaaaat agcggctgtt atattcccgc
     8521 atcagctccg ctttcagctt ctcgttcacc gcgctttccc cgtgccgctc gctgaccacc
     8581 tcaatcatcg cgttaatctg ctccggtgag cacttcccgg catcaaccac ctgcacaccg
     8641 ttctcgatgg cgaaccgctc cacctttcga tagaggtaat cacacagctt cattcctgtg
     8701 ggtttgtccc gttcccattg cggattttca ccggtgaaaa agtacgtggc tttttcgtcg
     8761 ctgttgagcc gctcgtcata catcccgatg gatttcatca tcgccagatg ctgggcccgg
     8821 tcgacaaaac agaaaacata cgccccggtc tgccaggcga gcaccaccat gtcttccaga
     8881 tgctcgctgg ttggcataat gcagcgctcg ccgtctttgt cttccacgtc ctcaataaat
     8941 accatgtaca gcgccataat ccctcactcc gttgtcataa atttaaagca cgcgaacagc
     9001 ctctcccggt ccagctccac cgttttgcgg gcctcgccgg tctgtggatt cacgatttca
     9061 tccaccgcca gaatcgcccg ctgtacccgg acaagcgtcg gccggtcagg gcagatgtac
     9121 cagatttcat cgatgccgtt cgcccggcgg ttgaacagat acccggacac cacttcctga
     9181 taccgcctgc ggctcttgac ggtgcgctct acctcaaatg ccaccgttct gccgtccagc
     9241 tccgccagcg cgtccggcac tttcatccct ttcagggcca tacggtgcag gtaccgccag
     9301 ccggtggccc cccggccctc catggagagg cgcagggact gcacggccag ccggtgcggc
     9361 agctgcgccg ccgacaccct tgacggctga aacggaataa cgtccgtcag cggctcgtcc
     9421 tcgtcgaacg agaacgccac cccgtgcggc gtcagccccc acacgctgac ctgacggccg
     9481 tcaaccgggt atttcgcccg gaccagcagc cctttcgcca ccagactgtc caggaagcgg
     9541 taaaccggct gtaccgccgc aatccccagc cgtcgcgcaa ccagctccgc cgtggaacac
     9601 agctcttccc gcagccagcg cagggtcagc cgtgttttct ccgcgttccg cgcctgccgc
     9661 ttcgcgtggc tgacgttgta ctgctccagt gtcatcagta aagccccgca aaatcatcat
     9721 ccgcttttgg tccgccgccg tcggccggag gcaggtactc cgccagcggg tccgttttcg
     9781 ccagcaccgg gaaggttttc agcgaaattt cccggcgctc aacccgcagc gggctgctga
     9841 acgccagccg cgccacgccc agcccggtca tcaccgcgaa cccgtcaggc aggtgttgca
     9901 gggtgttcac gtcaaacagc ggccgcgcct ccttctggat gaagctgtcg ccgctgatgt
     9961 gctccacgtt ccccgcctcc cggctggccc ggcggcgctc cacgtccacc cggatctccc
    10021 cggtctggcc cgccgcccac tgcgcggact cctggctggc
//
