LOCUS       CP148307                5353 bp    DNA     circular BCT 25-MAR-2024
DEFINITION  Escherichia coli strain Z1323PEC0003 plasmid pZ1323PEC0003_1-5,
            complete sequence.
ACCESSION   CP148307
VERSION     CP148307.1
DBLINK      BioProject: PRJNA1087359
            BioSample: SAMN40441610
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/18/2024 14:38:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,074
            CDSs (total)                      :: 4,950
            Genes (coding)                    :: 4,410
            CDSs (with protein)               :: 4,410
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 540
            CDSs (without protein)            :: 540
            Pseudo Genes (ambiguous residues) :: 0 of 540
            Pseudo Genes (frameshifted)       :: 324 of 540
            Pseudo Genes (incomplete)         :: 222 of 540
            Pseudo Genes (internal stop)      :: 54 of 540
            Pseudo Genes (multiple problems)  :: 56 of 540
            Pseudo Genes (short protein)      :: 4 of 540
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323PEC0003"
                     /host="Dog"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323PEC0003_1-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-03-08"
     gene            join(3950..5353,1..300)
                     /locus_tag="WHU63_25315"
     CDS             join(3950..5353,1..300)
                     /locus_tag="WHU63_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005524 - ATP binding [Evidence IEA]; GO_function:
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WXN17564.1"
                     /translation="MIKVQLPTREHHHHELNLNYGDVVFFIGQNGSGKSSLLNNLFQKI
                     GSQSSVYVLAHRKNTFSSDIIDFSNSEYKNAQGWIFNEIKGSQCRYRVNYDSQMVAIPI
                     VKLKNKEIARALSFVDSFKSNNNQPAELIKSEVDVLNMVFADAKLSIEFFLDDEANVMA
                     RNIKGEHPDVYPISMLSDGEKSALIIASEIICADENTTIFLDEPERHMHRSIVSPLLSS
                     LMKYRRDCVFFVATHELSLPAFFDNVKVVNVKNCYFSEQREPMCWDFNVVEDYDELFNG
                     VDEQTKIDILGARDKVLFVEGVTSSLDRDLYSAIFPMVSVVSKQKCDLVELAVKGLRLN
                     DEIHRVSAFGIIDNDNKVQAKVDNLKKDFIFSLDVHSIESIYYHPRMIKFVIDFVKEAN
                     GIDNVDTLFMEIHDFAIDAINEKRDHLCCRAVEKTIRADLMSSIPKQQDIKNRIKFNKE
                     IDIECYVNKEIECYVNKEIAAFDAMIAERNLEALVSRYPIRETNLLAHVAKKCGFADRS
                     RYELNVIRTIKSNADARAFVLSLLGGLDEAILGCSDTENSPADTNTVFAM"
                     /name="AAA family ATPase"
     gene            complement(537..731)
                     /locus_tag="WHU63_25320"
     CDS             complement(537..731)
                     /locus_tag="WHU63_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WXN17565.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVHFEAENETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1657..2124
                     /locus_tag="WHU63_25325"
     CDS             1657..2124
                     /locus_tag="WHU63_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011899450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXN17566.1"
                     /translation="MPAPNNYGVAGSPNSAIAHPDGLDCYGRVPEINSKGDVIPAGDIF
                     LRVGRHIGPNRGFGVRHIWAEHEQELVKLGYATVNDVARFVRDIIRRGVPIYCEFNSTS
                     GKHRPAVLKSSIGIVILEPREAPETESGWIYVVVTAYTRRTAHGTLVGHIE"
                     /name="hypothetical protein"
     gene            complement(2718..2801)
                     /locus_tag="WHU63_25330"
     CDS             complement(2718..2801)
                     /locus_tag="WHU63_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WXN17567.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="replication initiation protein"
     gene            3370..3953
                     /locus_tag="WHU63_25335"
                     /pseudo=""
     CDS             3370..3953
                     /locus_tag="WHU63_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          971..1227
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.595"
                     /coverage="89.0"
                     /evalue="7.36e-109"
     Typing          complement(2643..3205)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.789"
                     /coverage="89.0"
                     /evalue="1.17e-159"
ORIGIN
        1 attgaatgtt atgttaataa agaaattgct gcatttgatg caatgatcgc ggaacgaaat
       61 ttggaggcgt tagtctcacg ctaccctatc cgagaaacaa atttgttggc acatgtagct
      121 aaaaaatgtg gttttgccga tcgctctaga tatgagttaa acgtgattcg tacgatcaag
      181 agtaatgctg acgctagagc atttgtttta tccctcctcg gagggttaga tgaagctatt
      241 ttgggatgtt cagatacgga aaatagccca gctgatacca atacagtttt tgctatgtga
      301 gactaaacaa cctatatcag ataacagccc tgcttttgcg gggctttttg tttgtgcgtg
      361 acttgaccgc gatgtacgat agttgctgta ttaagtcagt atacactccg ctatcgctac
      421 gtgactgggt cagggctgcg ccccgacacc cgttaaaacc cgctgacgcg ccctgacggg
      481 cttgtctgct gacgaaaatg ctcacgggca agccgtgacc gacttcgtca gcttcgttat
      541 gttcctgttt cattttcggc ctcaaagtga acctggatgc tgttctggag ttcttctgcg
      601 agttcgtgca gtcgttcaca catggccgcc tgttcgtcgg catccagagc atccagtttt
      661 tcaagcagtg ttaggctctg gcttttgata aatcgcgcca tattcagcgc agtttgttgc
      721 tgtttgttca ccttacgcgt tctctgtcgg tgtcggttgg tccctgacta ggtggtaatt
      781 gtaagaggtt gcatgcgacg gtattacaat tgcatatcct gtcctgtttc ttcaggcagt
      841 attatggccg gataaataat acctgtgcac aacagccgcg gttgcataaa atctcgctct
      901 gcggcaagtg ttttttaatc aaacattccg ttgatactcc ggaaaaacac gagtctgttc
      961 tcatgcctga aatgcccaca ccccacgcaa aaacaagttt ttgctgattt ttcttttata
     1021 aatagagagt tatgacaaat tagttgctct tactctcttt gtgatattta aaaaagcggt
     1081 gtcggcgcgg ctacaacacc gcgccgacac cgctttgtag gggtggtact gactattttc
     1141 ataaaaaaca tcattttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
     1201 atgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
     1261 cacgaactga aaatgtccga aaatgtgtct catgggcacc gtatgcgccc aaaccgttca
     1321 ctattcactt tcatgaatag ctatgcagcg agctgaaaac gatcctgacg cattttttcg
     1381 gtttgccttg gggaaaacat ctctttttgc ggtatttgcg tcagaatcgc gttcagcgcg
     1441 tttctgcggt gcgggcaacg gatgttatgg taaatctagc tgctagggcg attcacacgc
     1501 catattttca ggtgtgatat ccttatgcac caaggatgaa aggacaaaaa ataatccata
     1561 aggattcttt ttatcgtctc tgcttttttc ttgggctcct tccgcttgtt tcctctctgg
     1621 taccatatgc ggactctact caataaggat cgttttgtgc ctgctccaaa caattacggt
     1681 gtggctggtt cacctaacag cgctatagct catcctgatg ggcttgattg ctatggccgt
     1741 gtccctgaaa ttaattctaa aggtgatgtt attcctgctg gcgacatctt cttgcgtgtt
     1801 ggtaggcata ttggccctaa ccggggattt ggtgttcgcc atatctgggc tgagcacgag
     1861 caggaactgg ttaagctggg atatgcgacc gttaatgatg tggctcgttt cgttcgggat
     1921 attattcgtc gtggcgtacc aatatattgc gagttcaaca gtacaagcgg taaacatcgg
     1981 ccagcagtgc taaagtcgtc gataggcatc gtaattcttg agcccagaga agcgcctgaa
     2041 actgagtctg gttggattta tgtcgttgtg actgcgtaca cacgaaggac ggcgcacggt
     2101 actttggttg gtcatattga ataaaaaaat agggcgctaa aaagcgccct ttatgaattc
     2161 gttggtagtc gcagcatttc cttagcacat agggttcaga aaccgacgct tgtcgtaact
     2221 acccggcaga tgaccagcca tctgactgcc cggcacagaa ggcctattac tggttgatgc
     2281 cccctgcgaa tgctcttgca gtacaataat cataaacatc gtgatgtaga tttgcaacca
     2341 acattttggt tgcaacctat atactaattt ttctcgataa cgccggaatc cattgctgtg
     2401 tctggctttc ttgaagctcc tgactataga taccctatat cctaacagtt attctgctgt
     2461 ggatatcaga aatgaacact tgagatcatc agtggctcgt cgtagtctct tgctcagaaa
     2521 cgaaaaaacc accctggcag gtggtttttt cgaaggttag ttaatcctgg cagattctct
     2581 aaccgtggta acagtcttgt gcgagacatg tcaccaaata ctgtcctttc agtgtagcct
     2641 cagttaggcc gccacttcaa gaactctcgt tacatctctc gcacatcctg cttaccaatg
     2701 gccgttgcca atggcgatta gtcgtgtcta tccgggttgg actcaagacg atagttaccg
     2761 gacaaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     2821 actgcctacc cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg
     2881 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc caccagggga
     2941 aacgcctggt atctttaagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattc
     3001 tgtgatgctt gtcagggggg cggagcctat ggaaaaacgc cagcggcgcg gccttttgcc
     3061 ctggctttgc ctttttgctc acatgttctt tccggcctta tcccctgatt ctgtggataa
     3121 ccgtattacc gtctctgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3181 cgagttagtg agcgaggaag cggaagagcg taggcatttg cattttctcc ttacgcatct
     3241 gtgcggcatt tcacaccgtc catggcgcac ttttcataca atccgcactg ctaccgcatc
     3301 gttaagtcag tactcacagc gtcccctctg cgaggtgcct tctgtgactg gttcaggggg
     3361 ctcgccgccc ccctcaaata tgctgccgcc attgagaatg cgctgcgtaa aaaattgggc
     3421 gccgatgcgg ggtattcagg gctaatttgc aagaatccga accacctgca ctggcagatc
     3481 accgtttggc agcctgagct ctaggccctc gactggttag ccgactacct cgaccttggc
     3541 gcagctaatg accgcgaaat cctaccggac tacggtttag gccgtaactg caccctcttc
     3601 gataaaaccc gcaagtgggc ttaccgtgct atccgccaag gctggccgca gtatgaccaa
     3661 tggctacaag cctgctttga acgcgctaaa gcctacaacc tgcagttctc cgcaccttta
     3721 gacgaaaacg aagtcatggg tattgccaaa agtgtagcta agtggactaa taaaaatttt
     3781 acagagtcat catttgatga ttttataaaa aagccccatt cggcagacat ccaatctcta
     3841 cgtgggaaaa aagtaaagga ggaggtagga cttcattaaa ctctccatgg attaatttag
     3901 gaattagtcg ttcaacgtat tttagattaa aagctaaaga gaaaggaaaa tgataaaagt
     3961 tcagttgcct actagagaac atcatcatca tgagcttaat cttaattatg gcgatgtcgt
     4021 tttttttatt ggtcagaatg gtagtggtaa atccagccta ttaaataatc tttttcaaaa
     4081 aataggatct caatcatcag tatatgtttt agcgcataga aaaaatacat tcagcagcga
     4141 cattattgat ttcagtaata gtgagtataa aaatgcgcag ggatggattt ttaatgaaat
     4201 taaaggcagt caatgtagat acagagttaa ttatgactca cagatggttg ccatacctat
     4261 agtaaaatta aaaaacaaag agatagccag ggctttaagt ttcgtagatt catttaaatc
     4321 aaataacaat cagccggcag agttaataaa gtcagaggtt gatgttctaa acatggtttt
     4381 tgctgatgct aaattatcta ttgagttctt tctagatgat gaagccaacg ttatggcacg
     4441 gaatattaaa ggtgaacatc ctgatgttta tcctatatca atgctttctg acggtgaaaa
     4501 aagtgcacta attattgcca gtgagattat ttgcgctgat gaaaacacaa ctattttctt
     4561 agatgagcct gagcgacata tgcacagaag cattgtgtct ccgctgcttt catctttaat
     4621 gaaatatcgt agagattgtg ttttttttgt tgccacccac gagttaagct tgcctgcgtt
     4681 ttttgataac gtgaaagtgg ttaacgttaa aaactgctat ttttccgaac aaagagaacc
     4741 gatgtgctgg gatttcaatg ttgttgagga ttatgatgaa ttattcaatg gtgttgatga
     4801 acaaactaaa atagatatac ttggcgctag ggataaggtt ctatttgttg aaggtgttac
     4861 gtctagcctt gaccgtgatt tatattcggc catatttcct atggtttcag ttgtatctaa
     4921 acaaaaatgt gatttggttg aattggcggt aaaaggcttg agactaaatg acgaaattca
     4981 tcgagtatcg gcgtttggta tcattgataa tgataacaag gtgcaggcga aggtcgataa
     5041 cttaaagaaa gacttcattt ttagcctcga cgttcattct attgagtcga tttattatca
     5101 tcctagaatg attaagtttg tcattgactt tgtaaaagag gctaatggga tagataatgt
     5161 tgatacctta tttatggaaa ttcatgactt cgctattgat gccataaatg aaaagcgcga
     5221 tcacttatgc tgccgtgcag ttgaaaaaac aataagggca gacttaatgt cgtccatacc
     5281 aaaacagcaa gatattaaaa accgcattaa gtttaataaa gaaattgata ttgaatgtta
     5341 tgttaataaa gaa
//
