LOCUS       CP146552                1552 bp    DNA     circular BCT 09-MAR-2024
DEFINITION  Escherichia coli strain B4.H1.17 plasmid pB4.H1.17.c41, complete
            sequence.
ACCESSION   CP146552
VERSION     CP146552.1
DBLINK      BioProject: PRJNA1082105
            BioSample: SAMN40199721
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 251x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/01/2024 13:05:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,490
            CDSs (total)                      :: 5,365
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 504
            CDSs (without protein)            :: 504
            Pseudo Genes (ambiguous residues) :: 0 of 504
            Pseudo Genes (frameshifted)       :: 240 of 504
            Pseudo Genes (incomplete)         :: 276 of 504
            Pseudo Genes (internal stop)      :: 57 of 504
            Pseudo Genes (multiple problems)  :: 65 of 504
            Pseudo Genes (short protein)      :: 4 of 504
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B4.H1.17"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB4.H1.17.c41"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2022-01-14"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(658..1134)
                     /locus_tag="V8960_00370"
     CDS             complement(658..1134)
                     /locus_tag="V8960_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WWW73097.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHINPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1214..1322
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          168..428
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="3.95e-106"
ORIGIN
        1 tgtaataccg tcacccgcga cgatattccg tttgccatct ggtcagggct tcgccccgac
       61 accccgtatc agtgcgtacg tgctcaatgt ggtttccagt tctcttccgc ttcctcgccc
      121 actcgctcac taacgttcgg tcgctggggc tgcggcgagc ggtgccacct ctcgcatgtt
      181 gtcggtgatt ccgctgttgt tctttcgggg atgagtcgtc agatagccct tcaaacgcgc
      241 tcagaagctc tcagacgcac gaggcggggt ttaaatgccc gtcagggcat ggaaggcgcg
      301 aaaaacgctt acagagcgtt tcaggaggtc aggcgcgccg atacccctag ccaggggctt
      361 tcaggtcgcc aggaaccgcg ccagccccct gcaaagccca tttgcacggt acaggcagtc
      421 actcggttgg taggtggtcg tagaccgtcg gtgttacagc gatgggggtc tggggttctc
      481 cccagacggt ttccggttgc gctcaaggcg ctgccggaac cgtggcggct cggtcgttct
      541 ggagatcggg gagcaaaaac acgcaaggga ttttgaggcg gcaaaagagc aatgcaggat
      601 agacaatcat ggtcagaacg ttaacacgta gcccctgatt gtccgcctgc cgctggctca
      661 atttgaaaac atggccatga ttcggcgctg aatatcaccg tctttgttga ccagtgtttt
      721 agtgacaaat gctgcatggt ttttcgtagt gttgtatccc ttccagactt catgtggatc
      781 gagggcatac gcacaaacct taccagcaac ggggattcta actaatgcat tttttgattc
      841 taagtaattg agccaacgaa taattgtgct tctgctaact ccaagttttt cagctaggaa
      901 ttgctgatcc gcaacgacag cgccacacgt aggattgata tgctccgcta aaaatgagta
      961 cagcttcagt gctgatatgc cttggctatc cttcagcagc tctcgaacgc gttcccaacc
     1021 ttttggggat acctgtgtaa accttgaatt cttctttgct tcttcgagat cctcggtgtt
     1081 tttctttgcc tcatctgaaa gttgtttcag tcgtttttct gaatcaataa acatgatttt
     1141 ttccctctaa aaatcccatg cactaacgtt ggttcaaatc taagccatca tgacacatct
     1201 gtgcaacacc ttgttgcgtg ttgtcgcatc ctggatgcga caacgttgtg tcaccctgga
     1261 tgcgacaggt aaacaaaaaa cacactaaaa aaacaacaac ttacaaatcg gcgtaaaaga
     1321 tctattctag gcaagggtgt tgcgcgcaac gcaacgccct ttgggggtgt cggggcgaag
     1381 ccctgaccca gtcacgtagc gatagcggag tgtatactgg cttactcctc tgcaagcctc
     1441 gccagccgtg atttttgcca gatttcgtgg ttttcacgtt caacagctcc tgcacgtttt
     1501 ttgccgtccg ttcaccgtta acccccgcag aaatcgggac aggatgtgca aa
//
