LOCUS       CP144328               12747 bp    DNA     circular BCT 11-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRE-137 plasmid pKP137-5, complete
            sequence.
ACCESSION   CP144328
VERSION     CP144328.1
DBLINK      BioProject: PRJNA1071866
            BioSample: SAMN39715938
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Tran,T.T.D.
  TITLE     Hypervirulent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12747)
  AUTHORS   Tran,T.T.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2024) Institute of Microbiology and Immunology,
            National Yang Ming Chiao Tung University, Linong St, Taipei 112,
            Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. Oct 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 19.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/05/2024 11:20:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,137
            CDSs (total)                      :: 6,016
            Genes (coding)                    :: 5,688
            CDSs (with protein)               :: 5,688
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 227 of 328
            Pseudo Genes (incomplete)         :: 114 of 328
            Pseudo Genes (internal stop)      :: 22 of 328
            Pseudo Genes (multiple problems)  :: 34 of 328
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRE-137"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP137-5"
                     /geo_loc_name="Taiwan"
                     /lat_lon="25.04118255 N 121.51920940 E"
                     /collection_date="2019"
                     /collected_by="Yu-Tsung Huang"
     gene            join(12722..12747,1..253)
                     /locus_tag="V3D01_30610"
     CDS             join(12722..12747,1..253)
                     /locus_tag="V3D01_30610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WVU71596.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            241..555
                     /locus_tag="V3D01_30615"
     CDS             241..555
                     /locus_tag="V3D01_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WVU71597.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            719..1147
                     /locus_tag="V3D01_30620"
     CDS             719..1147
                     /locus_tag="V3D01_30620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WVU71598.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1173..1352
                     /locus_tag="V3D01_30625"
     CDS             1173..1352
                     /locus_tag="V3D01_30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WVU71599.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1379..1909)
                     /locus_tag="V3D01_30630"
     CDS             complement(1379..1909)
                     /locus_tag="V3D01_30630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WVU71600.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1916..2648)
                     /gene="mobC"
                     /locus_tag="V3D01_30635"
                     /pseudo=""
     CDS             complement(1916..2648)
                     /gene="mobC"
                     /locus_tag="V3D01_30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /name="mobC"
     gene            complement(2648..3478)
                     /locus_tag="V3D01_30640"
     CDS             complement(2648..3478)
                     /locus_tag="V3D01_30640"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WVU71601.1"
                     /translation="MLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVAGIIN
                     GGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLSKYVL
                     NALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQESAQW
                     AAGQTGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGL
                     GVARLAFSSPLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3442..4611)
                     /locus_tag="V3D01_30645"
     CDS             complement(3442..4611)
                     /locus_tag="V3D01_30645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WVU71602.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGQTFSRCWRRRTRCRRP"
                     /name="hypothetical protein"
     gene            complement(6095..6214)
                     /locus_tag="V3D01_30650"
     CDS             complement(6095..6214)
                     /locus_tag="V3D01_30650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WVU71604.1"
                     /translation="MGVITGFLLAACQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6215..6912)
                     /locus_tag="V3D01_30655"
     CDS             complement(join(6215..6663,6663..6912))
                     /locus_tag="V3D01_30655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WVU71603.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(7106..7363)
                     /locus_tag="V3D01_30660"
     CDS             complement(7106..7363)
                     /locus_tag="V3D01_30660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WVU71592.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7373..8887)
                     /locus_tag="V3D01_30665"
     CDS             complement(7373..8887)
                     /locus_tag="V3D01_30665"
                     /inference="COORDINATES: protein motif:HMM:NF015479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WVU71605.1"
                     /translation="MHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLP
                     ALATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMM
                     SKIMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDA
                     KPTKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAV
                     IGFPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAEL
                     DAEDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQ
                     DQVRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKL
                     NEEKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRK
                     IYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(9164..10650)
                     /gene="ltrA"
                     /locus_tag="V3D01_30670"
                     /pseudo=""
     CDS             complement(9164..10650)
                     /gene="ltrA"
                     /locus_tag="V3D01_30670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]; GO_process: GO:0000373 - Group II
                     intron splicing [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="ltrA"
     gene            11295..11540
                     /locus_tag="V3D01_30675"
     CDS             11295..11540
                     /locus_tag="V3D01_30675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WVU71593.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11814..12185
                     /gene="tolA"
                     /locus_tag="V3D01_30680"
     CDS             11814..12185
                     /gene="tolA"
                     /locus_tag="V3D01_30680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WVU71594.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            12182..12547
                     /locus_tag="V3D01_30685"
     CDS             12182..12547
                     /locus_tag="V3D01_30685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WVU71595.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             2373..12747
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5414..5992
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1920..2539)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.237"
                     /coverage="85.0"
                     /evalue="2.8599999999999997e-126"
ORIGIN
        1 cgatgaagaa cttaaggacc ggctgaaaac gctggccgat gaccggcacc gctcagcgca
       61 cgcgctaatg ctggaggcaa tcaccgaata catcgaccgg gaagagaagc gcagccagta
      121 cctgcgggac ggacaggccg catggcagca ctatcaggaa acgggtctgc accttactgc
      181 ggaagaagcg gaagcctgga taagtacctg gggaactgag aacgagcagg atgctccgcc
      241 atgccacagg taacgatttc cgcgctggca cagcgcgatt tacaacgcct tcaggacttt
      301 ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg aggtgatcgt ccgggctatc
      361 cagcaactga aaacattgcc agacattggc cgcccggtgc cgtttctgcc gctggaatat
      421 aaggaactgg tgatcgggtt tggcgacagt ggctatgtga tgctctaccg ccacgaccgg
      481 gaaatggacc agattgtgat cgtcaccgtc aggcatcaga aagaatccgg gtatccgggg
      541 gcagacagcc tctaataacc gatgacgggg gcgcaattca ttgcgtggcc gtcgccggtt
      601 tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga aaaacgggta aaattcagcg
      661 atacatttac cgtgagaaat tacacacctt tcacacccga taaaatcagg ggtatacagt
      721 ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc gcgggcatta ccagaagaac
      781 gctatacaat catgtaaaac agggaaaggt taccgtgtca cgtgacggga aaaacaaccc
      841 tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat gtgaatatcc ctgagaaaca
      901 aattcccggc atttcacacc gtgaaaatac acaaaaaaat ttcccacagg aacagcttct
      961 ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc accctgatgc tggaagataa
     1021 aacatcccgg gaagaagaac gccgccagca cgatgatgag cgccggaaac tccaggccga
     1081 agtggacaga ttgacaacag aactgacaca gaagaaaaaa cgcttctggt ccggctggtt
     1141 tagctgagaa cttaccgaag ggaatggaga acatgacgaa gcaggaaaaa actgccctca
     1201 atatggctaa attcattaag gatcagtcgc tgctgttgct ggaaaagctg aacgagctgg
     1261 acatggatac cccggccgac atgtgcgaac ggcttcacga ggatgcggaa acactctggc
     1321 aggtgatgcg ggataagctc ggggaagaat aacagcaccg gccatgccgg tgctggcgtt
     1381 attttttgcc gaacggccac caccggcggg cgggtttgtc ctccggcagt tcaggcaccg
     1441 gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc ttctcgttca
     1501 ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat cgcgttaatc tgctccggtg
     1561 agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc tccacctttc
     1621 gatagaggta atcacacagc ttcattcctg tgggtttgtc ccgttcccat tgcggatttt
     1681 caccggtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca tacatcccga
     1741 tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg
     1801 tctgccaggc gagcaccacc atgtcttcca gatgctcgct ggttggcata atgcagcgct
     1861 cgccgtcttt gtcttccacg tcctcaataa ataccatgta cagcgccata atccctcact
     1921 ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg gtccagctcc accgttttgc
     1981 gggcctcgcc ggtctgtgga ttcacgattt catccaccgc cagaatcgcc cgctgtaccc
     2041 ggacaagcgt cggccggtca gggcagatgt accagatttc atcgatgccg ttcgcccggc
     2101 ggttgaacag atacccggac accacttcct gataccgcct gcggctcttg acggtgcgct
     2161 ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag cgcgtccggc actttcatcc
     2221 ctttcagggc catacggtgc aggtaccgcc agccggtggc cccccggccc tccatggaga
     2281 ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc cttgacggct
     2341 gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa cgagaacgcc accccgtgcg
     2401 gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg gtatttcgcc cggaccagca
     2461 gccctttcgc caccagactg tccaggaagc ggtaaaccgg ctgtaccgcc gcaatcccca
     2521 gccgtcgcgc aaccagctcc cgccgtggaa cacagctctt cccgcagcca gcgcagggtc
     2581 agccgtgttt tctccgcgtt ccgcgcctgc cgcttcgcgt ggctgacgtt gtactgctcc
     2641 agtgtcatca gtaaagcccc gcaaaatcat catccgcttt tggtccgccg ccgtcggccg
     2701 gaggcaggta ctccgccagc gggtccgttt tcgccagcac cgggaaggtt ttcagcgaaa
     2761 tttcccggcg ctcaacccgc agcgggctgc tgaacgccag ccgcgccacg cccagcccgg
     2821 tcatcaccgc gaacccgtca ggcaggtgtt gcagggtgtt cacgtcaaac agcggccgcg
     2881 cctccttctg gatgaagctg tcgccgctga tgtgctccac gttccccgcc tcccggctgg
     2941 cccggcggcg ctccacgtcc acccggatct ccccggtctg gcccgccgcc cactgcgcgg
     3001 actcctggct ggccgcgcga tagaaccagc ggatcggcgt gttatccagt accgtggttt
     3061 tcacaaaatc agcgggcaga tcctgcccgc actgaccgaa gtcgcccagc gactggtgcg
     3121 cgagcagcag gttgcagtcc cggctgcgca gcgtccccag cgcgttcagc acgtacttcg
     3181 acagcagata tttaatctca tcgagcatga tgctcgcgtg cggccacctc cggaactcat
     3241 cccgcgcaat gatgatttgc gcgcagcggg caaacagcat cttctgcacc cggatcaccg
     3301 cctcatcgtc catcgagccg atgacgtaca ggcagccgcc gccgttgatt atccccgcca
     3361 catcgatacc gctgtccgtc tgcaggacgc tcaggtcggc cacgttctcc agcttggtga
     3421 tcagcccctt cacgtccttt ttcagggcct ccggcaacgc gtacgccgcc gccagcacct
     3481 gctgaatgtt tgcccctgcg ggaactgctc cgcaatcagc ttcgccgctt tctgctcgct
     3541 gatgcggtag tggtccgcca cgtcgccggt ctcactcagg ttaaacccgg caaccagcag
     3601 attcttcagg gcgtactggt cgagatcgcg gaacagattc agctgcgccg gtttccccgg
     3661 ccgcagatcc agcagggtga agtccgggca gtgcgcccgg aagacgtgcg gcgcccacgc
     3721 gtcccctttg gggtcgaagt acaccacagc gtccccgaaa tcgcgtacgc agcgaatgag
     3781 cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg ccgaggatct gaatattggt
     3841 cttgcgccag gtatcacgag ggaccagcac cggctggccc tgctcatcct tacccagaaa
     3901 caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg gccagcgcgt taaccggtac
     3961 cggctccccg aaatgcaggt ggcggacgtc tgtcatttct ccggtccgct ggctgctgcg
     4021 ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcggccgcaa aatgaccatc
     4081 aagcagcagg aacgccagca gccgcgccag aaggacgccg gcgacaagcc ctccggcaca
     4141 ccaggctatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt tcgcatagag
     4201 gtccgggcgc aggacggttt ctccccagct ctcgataaac acggcgacaa ccagcccccc
     4261 cagaagggcc agaccggcag cgccggtcag ccagtacgcg tagttccaga catgtcgccg
     4321 tgccctgacc atccgcccgg gtgaatccag cgaggccagc accgaaaatg gcacagtccg
     4381 gttattatcg cgcttatagc gatcggcata ctcccgccgc gccttatggc tcagccccac
     4441 aaaacgcatt cggccggccc agagttcagc cagaccgaaa aacaggccga taaccagaaa
     4501 aaaaccggcc agactgtaat actcccggta cagccagctg acccacgggt aaatctgccg
     4561 ggccagctcc gggatatgcc actgccaggc aagcgaatcc gtattaaaca tgcaatctct
     4621 ctccttcaga cgacgggttt accggcgtga tgcgcagccc ccgctgcaca agccgcggac
     4681 atgccccaag gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccgc
     4741 ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca
     4801 cgccccagcg tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc
     4861 ccccgtcaga gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc
     4921 tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac
     4981 gggggccggg tgaatttctg gcgtggccgg cgtccagccg gacagagcca gaaagagggg
     5041 aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa ggtcttttcc cctgaagtca
     5101 gccacgcccc agcggggatg acttgcattt ctccgcaagg agaaaaaagc aacagagata
     5161 ggttaacgta aaatgttcag cgaaaaaccg acatggttca cctatcctga taattgatcg
     5221 tcagggaata gaaagactta atcaggggac aatatcccac atcagcgcgt ccggtgaccg
     5281 tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccgggcgt
     5341 gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca
     5401 ggggactaac atgtgaggca aaacggcagg gccgcgccgg tggcgttttt ccataggctc
     5461 cgccctcctg ccagagttca cataaacaga cgctttttcg gtgcatctgt gggagccgtg
     5521 aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc
     5581 gtttccggta ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata
     5641 cctgtccgcc gttctccctt gcgggaagtg tggcgctttc tcatagctca cacactggta
     5701 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
     5761 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg gaaagcacgc
     5821 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
     5881 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
     5941 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
     6001 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
     6061 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt
     6121 cagaggagga cgatggtgcc accgttccgc cctgaacatc ccggatataa tttgcctgac
     6181 atgcggccag aagaaagccg gtaatgactc caatttactg atagtgtttt atgttcagat
     6241 agtgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagg gacttcctgc
     6301 ccagccttgc cagatgctgt ctcagattca ggttgtgtcg ctcaattcgc tgcgtgtaac
     6361 gcttactgat gacgtgcagt tttcccttca gacgtgactc atacatcggc cagccgtccg
     6421 tcatccatac caccacgtca aaaactgaca gcaggctcag aagacgctcc agtgtggcca
     6481 tagtgcgttc accgaaaacg tgggctacca ccgccctgcg tatcctgtca tacgcgtaaa
     6541 acagccagcg ctgacgtgat tttgcaccga cgtagcccca ttgctcgtcc atctccgcac
     6601 agacaatgac atcactgccc ggctgtatcc gggaggttac tgactgcggc ctgagttttt
     6661 taaatggcgc aaaatggtgt tgaggcccac gcccattagt cgtgcggtgg cacggcaacc
     6721 gacgccgttc atagccatat caatgatttt ctggtgtgta ccgggctgag aagcggcata
     6781 agtgaaggtg agctgccatg ttttacggca gtgagagcag agataacgtt gatgcccggc
     6841 agtacttttg ccattacgca tcacgccttc agtagcggaa caggagggac agcagacgga
     6901 gactgaagcc acgggagcac ctcaaaaaca ccattataca ctaaatcagc aagttggtag
     6961 catcaccaag aaagccagca acaatcaata taaaaagaaa aatagtcttt acctttttca
     7021 tatactttcc ctgtatttaa agactccggc attcatccct gaacaccgga agtcagtatt
     7081 tcaaatcatc cttgataatt ttttgttacc agttaccgtc ccgataatca aaagctatat
     7141 aacaattatc cacatcaggt tttatttgat ttttaaaaaa tggctgtagt aaaggaaccc
     7201 attctttctt caaatcaaac tcaccattat taatattatc ctttaaaggc attcccaagc
     7261 tttcaatgac agaaccatcg tcaccaaagt ctttagaata ctcttcccct ttaaactctt
     7321 cagttttctt atcaaaccat gtcaaacgta atttaagccc catatttacc tctcaaagat
     7381 attttttaat gttccgtttt ggatctggcc ctttaacctg cttacccgtt ttgggatcaa
     7441 atgcgcctag gtgttcgcca tcactggcac gatacccttc aagctcaccg tgctgggaat
     7501 cccattcata aattttacgt tttttatcgc cataccatcg ggcccgctta ccaccaccac
     7561 cttgtttagg tgttttagga tcacctcgct ttaactctcc aagtcccttt atgtcttctg
     7621 ttttaggtac tggatgataa tcatggccgt agtctttagt ccccttgcga ggcttgttct
     7681 tttcctcatt taacttattt tctgcatcct tcgctttttt ctccttttgt ttccggctct
     7741 ccatcgcccc ggtaagtgca gccttatctc tggataagga ttcattcatt gcgtttaaag
     7801 atgcctgagc cgcttttaat ttctcattct gattctgaat atccttatca agatagcgaa
     7861 cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca aaatcatctt
     7921 tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc cttgcaccgg
     7981 ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg
     8041 catccagctc agctttcgct ttatcatagt ctcttttcag cccctcttcc ggatgagcgg
     8101 cgtcccatgc ctgctggcga cgcttttctt cctcctgacg ctgttttacc tgtgccgggg
     8161 taagaacatc tgtcaccgac acataaaccg gcttctgtcc ggtctctttc gggaaaccaa
     8221 taacggcctc acgggagtta ccaccggctg taaaaccagc cggacgtgaa tcaccttttt
     8281 cagcaacaat gccttttggc ggggctttcg ctgtcggaac acctttcggt acgctcacct
     8341 gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt gtcggtttcg
     8401 catccaccac agggacactc attggccgac ctgcgacaac cgcaatatgc tgccgctcat
     8461 ccttcaccac atccacaacc cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag
     8521 tacttgccgg agtctccgtc accgcatcgg ccggtaatga cgtcataatt tttgacatca
     8581 ttttcggatc atcttttgca atctcagaag gcagcacacc gtagatcgca atcccccaca
     8641 gaccaaactt aaacggcccc ttcagggcag ccagcacatc agcaacggcg gccgacaacg
     8701 catcgccgga aactgataaa gccagtcctt cagcccccgg agtagccagg gcaggcagac
     8761 cgaaggccat cgcagtggcc gaagactgtc cgccaccaga gctaccgcta tgattgccgc
     8821 tgttaccacc attaccaccg ggtttactcc cgccgttacc aaactccccc ggattatagt
     8881 tatgaatatc tccattcggc gtatgcgtcg taccccagcc cggcccgctg tttggatccg
     8941 taccaccacc tgatgaaggg ccacctttac caagacgtac cgttcacatt cccactggcc
     9001 tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc accactcata
     9061 ccgttttcct ctttgacatt taaaacaata aattaaagcc aacatgtcgc ggtagggata
     9121 cccgttaccg gatacccccc gcacagatcc cggcgtgcgc gatttacgca ccgggctcct
     9181 gcctcgggtg tctggcggtg aaccgctcca caggccatgg atgaagaacc cgaacccttg
     9241 gtagccatgc ggctgccagt ttgtttgctt tcgtccaggt cgtatcatcc ttctggctcc
     9301 tgcgcctgag cgcccggcgc cagaggtttg ttacgtgtgt cctgaacttc tgcatggtgg
     9361 ggaagttgcc cggtaccgag tgatagttca ggtatccctg aaccactctc ctgagccatt
     9421 ttccctgttc ggggattgag taatgccagc gccttcgcag accgtctttg atggctttca
     9481 gagttgccgt catccgatcc cggcgggtct ttcgtatcag catgaacctg ccgttgcgat
     9541 ctttcccgct gatgtgcgtg aacccgagga agttgaacgt ttctggtttg ccttttcccc
     9601 tgatggcacg gttttcggca gcgaagcggc cgaactccat cagacgggtt ttctccgggt
     9661 gaaccgtgag tccgaactcc ctcagtctgc gctgcatggc tatacggaag cgccgggcat
     9721 cgtatcgttt gtcgaacccg atgacgatgt catcggcgta tctgaccatt accacattgc
     9781 ctgtggcata gcgacgtcgc cactgatgcg cccacagatc gaagacgtag tggaggtata
     9841 tgtttgccag cagcggtgag atgaccgcac cctgtggggt gccttcctcc gttgctcgcc
     9901 attgaccctc ctccgacgtc ccggctgtga gccacttacg tatgagcctg attaccctcc
     9961 ggtcgccgat ccgatgctct gtgaacctga tcagccattc gtggctcacc ctgtcgaaga
    10021 actgactgat gtcggcatcc agtacccagt ttacgttagt gcgtaccagc cctgtggcca
    10081 gtgcgtccag tgcatcgtgc tggcttcgcc cgggtctgaa cccgtatgag aaccccataa
    10141 agtcgttttc atagactgcg ttcaggattt tcaccagcgc atactggacg atcttgtcct
    10201 ccagcgaggc gatgccgagc gggcgttgtt ttccatccgc ttttgggatg tagtgacgcc
    10261 tgccgggctg cgccctgtag ctgccctgat gtagcctccg gtgcagatct gttatgttgt
    10321 tcttcatgtt tccggcgtag tccatccacc tgatgccatc cactccggcg gccgctttcc
    10381 tgctcaggga gaggaatgcg gcttccagtg cttcgactgt cagcaggtgg aacaatgctg
    10441 taaaccgttc ttcttccgct gcttcgcagc ttcccgcacg cgtgacagcc tctgtgacat
    10501 gctttcccgg ctctgtgtcc ggcgcatgtg tggctgttcc gcgttcccct tggccccgct
    10561 ccttcgctcc actgactccg ctcctttcgg gttgttcgcc tgcttcgccg ctactatgag
    10621 cgagtccgac ttctcctctc cgtacatcac cggctatgac tcctcgtctt cccggtgcgg
    10681 gccatctccg acactggcag atggtcagag gggagatctc ccggttcccg cgtagagatc
    10741 gtattgacat gccagggtct cagaccccgc cgggtccatg tggcactcgc agtatcgcac
    10801 cctatgatgt tgccttccgt taacagtaca acgtcggcac ccggtaattt aatatacatt
    10861 tcgtggctca atggctggcc tgtcaacacc cctgtcaacg cttcgcccca tacctcgcgg
    10921 tatgcaacgc atgactcggg gaccttgtgg attgctggtc cttcaatggt cggggacttt
    10981 caccccttga tctctaaccg gtctcccggc gcacactgta catatacaca gtataattat
    11041 taccttactc ctcccgtttt caggtgtcaa tatatgagat tagttctcat cttcaggcac
    11101 aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt ttcccgaaaa tttctcatgg
    11161 caacaaattt tattatttaa gacattcatc acacttacat ttcagatggt atagacacat
    11221 ctgaaagcta actggcatga ggagaggacc tgcggttaaa ctgttcggga aacctgagac
    11281 gcccggaggc cactatgacg cacctgaaac tgactcccgc tgaagaaaag cgcctggcgg
    11341 acgcctttgt cgctcaggca ctggccatgg gcctgaaagg gaacaaaacg ctgccaaagg
    11401 cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat tcaggacgaa cgtgaagcca
    11461 ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac ggaaaccacc ttcacctggc
    11521 agccacagcg ccgccggtaa ggggtattta ccataacgtc gcattgcccg caccgctcag
    11581 acggtctgag ggcgtttccg gcggctacgg gataaaacag gcaacaaacc acccggaaaa
    11641 ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg cctatgcacg caactgcatg
    11701 gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt tatgtaaatg aacgtggccg
    11761 taaagagtga caaatataca tgtaacattt tttactgaca ggtcatcaac ccgatgcgtt
    11821 taataccctt tatcctgctg gcattgtgca gccccttact cctgacggcc tgtaagcatt
    11881 ccaccaccca gccgccgaaa atttatactc tggataagtc atctaaaatc agtagctacg
    11941 ctgcggccat caagaccgct gttgaaatgc agatgtttga accagaggcc tatgcgggaa
    12001 aaatgtgcac cgtgcggata tccatacaac gtgatggcac tctgaacagc gccattgctg
    12061 aaggaggaga tccgaaactc tgtaaagcag caatatcagc cgttacccac gccaaaattc
    12121 cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa cgcccctctg gatttcagac
    12181 catgaaagct tttactctga cagtgctggc ggccatgttc ctcactccgg tacatgcgat
    12241 accactaaaa cccgtaacca cactgcagcc aacctacccg gtacaggccg cagcaaaacg
    12301 catcgaaggt caggtgaccg ccacgtttga tattctggac gatggacgag tggacaacgt
    12361 gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg acggcaatga ggcactggct
    12421 atatcccgca gggcacccgg caatgcgaca ggttataaat tttgaattca gtctgaaaaa
    12481 tgacggctta caggatccgc gaccagtcac cacccaaaag ctcaatgatg atggttgccg
    12541 ccagtaacag gtcctttaac ccgcaggctg caatagtcct ggatgatgag tgagcctgta
    12601 gcagccctga ttagtggaag cgaagctgaa aaatgggcag caaaagccgt atttctggca
    12661 aatagcgccg ccacggatta aagtagtcat gtgtaataca tatgactaca aaggggcttt
    12721 tatgtcatca gcaacatcca tcaggct
//
