LOCUS       CP143336                5282 bp    DNA     circular BCT 01-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2023CB-00606 plasmid unnamed5,
            complete sequence.
ACCESSION   CP143336
VERSION     CP143336.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN38182633
KEYWORDS    .
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/25/2024 15:42:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,254
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,987
            CDSs (with protein)               :: 4,987
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 52 of 147
            Pseudo Genes (incomplete)         :: 102 of 147
            Pseudo Genes (internal stop)      :: 13 of 147
            Pseudo Genes (multiple problems)  :: 19 of 147
            Pseudo Genes (short protein)      :: 1 of 147
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2023CB-00606"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..904
                     /gene="ltnD"
                     /locus_tag="V1238_01840"
                     /pseudo=""
     CDS             1..904
                     /gene="ltnD"
                     /locus_tag="V1238_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]; GO_process: GO:0005975 - carbohydrate
                     metabolic process [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /name="ltnD"
     gene            916..2166
                     /gene="otnK"
                     /locus_tag="V1238_01845"
                     /pseudo=""
     CDS             916..2166
                     /gene="otnK"
                     /locus_tag="V1238_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /name="otnK"
     gene            2159..2590
                     /locus_tag="V1238_01850"
     CDS             2159..2590
                     /locus_tag="V1238_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="WVJ08743.1"
                     /translation="MTEQQLREEMVQIGASLFSRGYATGSAGNLSLLLPDGNLLATPTG
                     ACLGELQAQRLSVVTLQGEWISGDKPSKEVTFHRAVYLHNPACKAIVHLHSHYLTALSC
                     LQGLGHCCKVSDEAAFCLIQRPYISKTLLTRRISPRGSP"
                     /name="class II aldolase/adducin family protein"
     gene            complement(2536..3240)
                     /locus_tag="V1238_01855"
     CDS             complement(2536..3240)
                     /locus_tag="V1238_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WVJ08744.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMPAQRGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            3509..4239
                     /gene="bla"
                     /locus_tag="V1238_01860"
                     /pseudo=""
     CDS             3509..4239
                     /gene="bla"
                     /locus_tag="V1238_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity [Evidence
                     IEA]; GO_process: GO:0030655 - beta-lactam antibiotic
                     catabolic process [Evidence IEA]; GO_process: GO:0046677 -
                     response to antibiotic [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="bla"
     gene            complement(4260..5021)
                     /locus_tag="V1238_01865"
     CDS             complement(4260..5021)
                     /locus_tag="V1238_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="WVJ08742.1"
                     /translation="MIPIERHQRILALVEQRGAVSINELTEILGVSHMTIRRDVSKLEE
                     QGLLVSVSGGVRAVSRLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTT
                     LALARAILDRNDLQVVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRH
                     LAIDTAFISASGWDSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPL
                     TRFTTIITDRHLSDAAASHIARHACEVLRAG"
                     /name="DeoR/GlpR family DNA-binding transcription
                     regulator"
     ARG             3998..4239
                     /name="SHV-4"
                     /gene="SHV-4"
                     /gene_name="SHV beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; cephalosporin; penam"
                     /identity="98.75"
                     /coverage="27.97"
     ARG             3378..3964
                     /name="LEN-14"
                     /gene="LEN-14"
                     /gene_name="LEN beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam; penem"
                     /identity="90.79"
                     /coverage="128.29"
ORIGIN
        1 atggctgcac acactaacgt ctgcgtgatt ggactgggtt caatgggcat gggcgccgcc
       61 cgcgcctgcc tgcaggcggg cctgaacacc tggggcgttg acatcaatcc cgacaactgt
      121 cgcgcactgc tggcggcggg cgccaaaggc gcgggcccca gcgcggtgcc gttcgccgcg
      181 gaactggatg cagttgtgct gctggtggtc aatgccgccc aggtgcgggg gatcctgttc
      241 ggcgagagcg gcctcgccgc ccatctgaag ccgggcaccg tcgtgatggt gtcgtccacc
      301 atcgcctccg ccgatgctca ggccattgcc gaggcgctgg cggagtacca gctattgacg
      361 ctcgacgcgc cggtatcggg cggcgccgtg aaagcggccg ccggcgacat gacggtgatg
      421 gcctccggga gcgatgccgc ctttgcccga ctcgcgccgg gcgctggacg ccgtggccgg
      481 caaagtctac cgcatcggga gcgacattgg tcttggctcg acggtaaaaa ttatccatca
      541 gctgctggcc ggggtgcaca tcgccgttgc cgccgaagcg atggcgcttg ccgcccgcgc
      601 cgggatccca ctggagacga tgtatgacgt ggtcacccac gcggcgggta attcctggat
      661 gtttgagaat cgcatgcagc acgtcctgga tggcgattac tcgccaaaat ccgctgtcga
      721 tatttttgtc aaagatctcg ggctggtgaa tgacactgcc cgggcgctga ccttcccgct
      781 gccgctcgct accaccgcgc tgaatatgtt cacctccgcc agtaatgccg gattcggtcg
      841 ggaagatgac agcgcggtga tcaagatttt caacggcatc accctgccgg gccataaaca
      901 gtgaggagag acaacatgca gcttggtgtc attgccgatg acttcaccgg cgccacggat
      961 attgccagct tcctcgtgcg caacggcatg ccgacggtgc aactgaatgg cgtgccgacc
     1021 cgcgatattc cgctgaccag cgaggcggtg gtcatcagcc tgaaaacccg ctcctgcccg
     1081 gcggaaatgg ccgtcagcca gtcgctggcg gccctgcgct ggctgcaggc ccagggctgt
     1141 cagcagtttt atttcaagta ctgttccact ttcgacagca ccgcgcaggg caacatcggc
     1201 ccggtgctgg atgtcctgct ggccgagctg ggtgagacgc ggacggtgat ttccccggcg
     1261 ctgccggtta acggccgcac ggtctatcag ggatatctgt tcgtcggcga gcaactgctg
     1321 aatgagtccg ggatgcgcca ccatccggtg acgccgatgg aggatgcgca cctgggccgc
     1381 ttaattgagc gccaagggcg cggaaaagcc gcgctgattg cctggccgat tgtcgcccgg
     1441 tcgccgccgc gctggcggca gtcaacgatc cggcggtgcg ctatgtggtg ctcgacgccc
     1501 tcagcgaaca ggatctgctc acccagggcg tggcgctgcg ggagatgaag ctggtctccg
     1561 gcggttccgg cctcgccatc ggcctcgccc gcgactgggc gcagcgccat ggcgccaggg
     1621 gagagccctc aggccggcat gccgctggcc ggtccggcgg tggtgctctc gggctcctgc
     1681 tcggtgatga ccaacagcca ggtggcggcc tatcgtcagc aggcccccgc ccgcgccgtc
     1741 gacttaagcg cctgctttac cgatctggag agctacgtca ggacgctgac tgactgggtg
     1801 gacgcgcagc gcgatgcgcc gctggcgccg atgatctatg ccaccaccga gccgcaaacg
     1861 ctgcagcgga tccaggcgca gtatggcgac aaggccagca gcgaacgggt ggaacagctg
     1921 tttgccgctc ttgccgccgc cctgaaggcg aacggattta cccgctttat tgtggccgga
     1981 ggggaaacgt cgagcattgt ggcgcagacc ctgggggttg aggcgttcca tattgggccg
     2041 accatctccc ctggcgtgcc ctgggtgcgt gacacccgcc agccgctctc cctggcgctg
     2101 aagtcaggta acttcggcga tatccagttc tttgcccgtg cccagcagga gtttcgtcat
     2161 gactgagcaa caactgcgag aggaaatggt gcagattggc gcctcgttgt ttagccgcgg
     2221 ctatgccacc ggctccgctg gcaatctgtc gctgctgctg ccggacggca acctgctggc
     2281 gacgccgacc ggcgcctgcc tcggcgaact gcaggctcag cggttgtcgg tggtgacgct
     2341 gcaaggggaa tggatctccg gcgacaaacc gtcgaaagag gtcacttttc accgggcggt
     2401 ctatttgcac aacccggcct gcaaggcgat cgtccacttg cacagccact atctgaccgc
     2461 gctctcctgc ctgcaggggc tcgggcactg ttgcaaagtt agcgatgagg cagccttttg
     2521 tcttattcaa aggccttaca tttcaaaaac tctgcttacc aggcgcattt cgcccagggg
     2581 atcaccataa taaaatgctg aggcctggcc tttgcgtagt gcacgcatca cctcaatacc
     2641 tttgatggtg gcgtaagccg tcttcatgga tttaaatccc agcgtggcgc cgattatccg
     2701 tttcagtttg ccatgatcgc attcaatcac gttgttccgg tacttaatct gtcggtgttc
     2761 aacgtcagac gggcaccggc cttcgcgttt gagcagagca agcgcgcgac cataggcggg
     2821 cgctttatcc gtgttgatga atcgcgggat ctgccacttc ttcacgttgt tgaggatttt
     2881 acccagaaac cggtatgcag ctttgctgtt acgacgggag gagagataaa aatcgacagt
     2941 gcggccccgg ctgtcgacgg cccggtacag atacgcccag cggccattga ccttcacgta
     3001 ggtttcatcc atgtgccacg ggcaaagatc ggaagggtta cgccagtacc agcgcagccg
     3061 tttttccatt tcaggcgcat aacgctgaac ccagcggtaa atcgtggagt gatcgacatt
     3121 cactccgcgt tgggccggca tctcctgcag ctcacggtaa ctgatgccgt atttgcagta
     3181 ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat
     3241 gtgcagctcc atcagcaaaa ggggatgata gagtttatca ccaccgacta gggcgccggt
     3301 aaccatgccg ggttattctt atttgtcgct tctttactcg cctttatcgg ccttcactca
     3361 aggatgtatt gtggttatgc gttatattcg cctgtgtatt atctccctgt tagccaccct
     3421 gccgctggcg gtacacgcca gcccgcagcc gcttgagcaa attaaacaaa gcgaaagcca
     3481 gctgtcgggc cgcgtaggca tgatagaaat ggatctggcc agcggccgca cgctgaccgc
     3541 ctggcgcgcc gatgaacgct ttcccatgat gagcaccttt aaagtagtgc tctgcggcgc
     3601 agtgctggcg cgggtggatg ccggtgacga acagctggag cgaaagatcc actatcgcca
     3661 gcaggatctg gtggactact cgccggtcag cgaaaaacac cttgccgacg gcatgacggt
     3721 cggcgaactc tgcgccgccg ccattaccat gagcgataac agcgccgcca atctgctgct
     3781 ggccaccgtc ggcggccccg caggattgac tgcctttttg cgccagatcg gcgacaacgt
     3841 cacccgcctt gaccgctggg aaacggaact gaatgaggcg cttcccggcg acgcccgcga
     3901 caccactacc ccggccagca tggccgcgac cctgcgcaag ctgctgaccg gccagcatct
     3961 ctgagcgccc gttcgcaacg gcagctgctg cagtggatgg tggacgatcg ggtcgccgga
     4021 ccgttgatcc gctccgtgct gccggcgggc tggtttatcg ccgataagac cggagctagc
     4081 aagcggggtg cgcgcgggat tgtcgccctg cttcgcccga ataacaaagc agagcgcatt
     4141 gtggtgattt atctgcggga tacgccggcg agcatggccg agcgaaatca gcaaatcgcc
     4201 gggatcggcg cggcgctgat cgagcactgg caacgctaag ccggcggtgg ccgcgcgcgt
     4261 tatccggccc gcagcacctc gcaggcgtgc cgggcgatat gactggcggc ggcatcggaa
     4321 agatgccggt cggtaatgat ggtggtgaac cgggtcaaag gtaacgccat aaacgtggcc
     4381 acctgattgt atttcgaact gtcgcacagc aggatgcttc tcgcgctgac ctggctgacg
     4441 gtctccttga cggtaacctt gttctcatca ggggtgaata tcccgcgact gtcccagccg
     4501 ctggcggaga taaaggccgt atcgatagcc aggtggcgta acgtacgcgc cgccgattcg
     4561 cccacgcagg agcggttctc ccggcacaga gtgccgccgg tgtggatcac gccgcactgg
     4621 ctggcatcga tcagcagctg ggttatctca aaatcattgg tgaccacctg gagatcgttc
     4681 cggtcgagga tcgcccgcgc cagcgccagg gtggtagtcc cggcatccag atagatgcaa
     4741 ctgtttttag cgatatgact cgccgccagc gcgccgatcg cctgtttctc ctcactctgc
     4801 agcgtgcttt tcaccagatg actgggttcc gcggccagcc ggctgacggc gcgtacgccg
     4861 cccgagacgc tgaccagcag cccctgctcc tccagtttac tgacgtcccg acggatggtc
     4921 atatgggaca cgccgaggat ctccgtcagc tcgttaatgc ttaccgcccc acgctgctcc
     4981 accagggcta aaatacgctg atgtcgttct attggaatca ccgctctccc cttaccattt
     5041 tttcacacca ggcgtcacca cccaggctac ggccctggcg acacccggtg ttgttatcgc
     5101 gctttgccgc tgttttttac gctattttag ggcaagaatc gccgctgtgc agcctctttc
     5161 cgcctgtgaa ttttttatat tcatgtgggt tattcgtgat aactctcaca taaattcaca
     5221 tgataacgct ttattctatc attaaatcac attaattaac taatgttcac aaggagacca
     5281 gc
//
