LOCUS       CP143332                5281 bp    DNA     circular BCT 01-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2023CB-00605 plasmid unnamed4,
            complete sequence.
ACCESSION   CP143332
VERSION     CP143332.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN38182632
KEYWORDS    .
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/25/2024 15:23:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,200
            CDSs (total)                      :: 5,080
            Genes (coding)                    :: 4,943
            CDSs (with protein)               :: 4,943
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 43 of 137
            Pseudo Genes (incomplete)         :: 101 of 137
            Pseudo Genes (internal stop)      :: 13 of 137
            Pseudo Genes (multiple problems)  :: 19 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2023CB-00605"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..763
                     /locus_tag="V1237_24130"
                     /pseudo=""
     CDS             1..763
                     /locus_tag="V1237_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226821.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /name="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            complement(784..1644)
                     /locus_tag="V1237_24135"
     CDS             complement(784..1644)
                     /locus_tag="V1237_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase SHV-12"
                     /protein_id="WVJ08046.1"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIEM
                     DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPV
                     SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWET
                     ELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLP
                     AGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALI
                     EHWQR"
                     /name="extended-spectrum class A beta-lactamase SHV-12"
     gene            1781..2485
                     /locus_tag="V1237_24140"
     CDS             1781..2485
                     /locus_tag="V1237_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WVJ08047.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSPRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(2538..2861)
                     /locus_tag="V1237_24145"
                     /pseudo=""
     CDS             complement(2538..2861)
                     /locus_tag="V1237_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226818.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /name="class II aldolase/adducin family protein"
     gene            complement(2854..4106)
                     /gene="otnK"
                     /locus_tag="V1237_24150"
                     /pseudo=""
     CDS             complement(2854..4106)
                     /gene="otnK"
                     /locus_tag="V1237_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /name="otnK"
     gene            complement(4118..5020)
                     /gene="ltnD"
                     /locus_tag="V1237_24155"
     CDS             complement(4118..5020)
                     /gene="ltnD"
                     /locus_tag="V1237_24155"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0016616 - oxidoreductase activity, acting on the CH-OH
                     group of donors, NAD or NADP as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="WVJ08045.1"
                     /translation="MAAHTNVCVIGLGSMGMGAARACLQAGLNTWGVDINPDNCRALLA
                     AGAKGAGPSAVPFAAELDAVVLLVVNAAQVRGILFGESGLAAHLKPGTVVMVSSTIASA
                     DAQAIAEALAEYQLLMLDAPVSGGAVKAAAGDMTVMASGSDAAFARLAPVLDAVAGKVY
                     RIGSDIGLGSTVKIIHQLLAGVHIAVAAEAMALAARAGIPLETMYDVVTHAAGNSWMFE
                     NRMQHVLDGDYSPKSAVDIFVKDLGLVNDTARALTFPLPLATTALNMFTSASNAGFGRE
                     DDSAVIKIFNGITLPGHKQ"
                     /name="ltnD"
     ARG             complement(788..1644)
                     /name="blaSHV-12"
                     /gene="blaSHV-12"
                     /gene_name="extended-spectrum class A beta-lactamase
                     SHV-12"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gtgattccaa tagaacgaca tcagcgtatt ttagccctgg tggagcagcg tggggcggta
       61 agcattaacg agctgacgga gatcctcggc gtgtcccata tgaccatccg tcgggacgtc
      121 agtaaactgg aggagcaggg gctgctggtc agcgtctcgg gcagcgtcag caccataagc
      181 cggctggccg cggaacccag tcatctggtg aaaagcacgc tgcagagtga ggagaaacag
      241 gcgatcggcg cgctggcggc gagtcatatc gctaaaaaca gttgcatcta tctggatgcc
      301 gggactacca ccctggcgct ggcgcgggcg atcctcgacc ggaacgatct ccaggtggtc
      361 accaatgatt ttacgagata cccagctgct gatcgatgcc agccagtgcg gcgtgatcca
      421 caccggcggc actctgtgcc gggagaaccg ctcctgcgtg ggcgaatcgg cggcgcgtac
      481 gttacgccac ctggctatcg atacggcctt tatctccgcc agcggctggg acagtcgcgg
      541 gatattcacc cctgatgaga acaaggttac cgtcaaggag accgtcagcc aggtcagcgc
      601 gagaagcatc ctgctgtgcg acagttcgaa atacaatcag gtggccacgt ttatggcgtt
      661 acctttgacc cggttcacca ccatcattac cgaccggcat ctttccgatg ccgccgccag
      721 tcatatcgcc cggcacgcct gcgaggtgct gcgggccgga taacgcgcgc ggccaccgcc
      781 ggcttagcgt tgccagtgct cgatcagcgc cgcgccgatc ccggcgattt gctgatttcg
      841 ctcggccatg ctcgccggcg tatcccgcag ataaatcacc acaatgcgct ctgctttgtt
      901 attcgggcca agcagggcga caatcccgcg cgcaccccgc ttgctagctc cggtcttatc
      961 ggcgataaac cagcccgccg gcagcacgga gcggatcaac ggtccggcga cccgatcgtc
     1021 caccatccac tgcagcagct gccgttgcga acgggcgctc agacgctggc tggtcagcag
     1081 cttgcgcagg gtcgcggcca tgctggccgg ggtagtggtg tcgcgggcgt cgccgggaag
     1141 cgcctcattc agttccgttt cccagcggtc aaggcgggtg acgttgtcgc cgatctggcg
     1201 caaaaaggca gtcaatcctg cggggccgcc gacggtggcc agcagcagat tggcggcgct
     1261 gttatcgctc atggtaatgg cggcggcgca gagttcgccg accgtcatgc cgtcggcaag
     1321 gtgtttttcg ctgaccggcg agtagtccac cagatcctgc tggcgatagt ggatctttcg
     1381 ctccagctgt tcgtcaccgg catccacccg cgccagcact gcgccgcaga gcactacttt
     1441 aaaggtgctc atcatgggaa agcgttcatc ggcgcgccag gcggtcagcg tgcggccgct
     1501 ggccagatcc atttctatca tgcctacgcg gcccgacagc tggctttcgc tttgtttaat
     1561 ttgctcaagc ggctgcgggc tggcgtgtac cgccagcggc agggtggcta acagggagat
     1621 aatacacagg cgaatataac gcataaccac aatacatcct tgagtgaagg ccgataaagg
     1681 cgagtaaaga agcgacaaat aagaataacc cggcgttggc actgttgcaa atagtcggtg
     1741 gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat tcaaaggccg
     1801 gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat acggcatcag
     1861 ttaccgtgag ctgcaggaga tgctggctga acgcggagtg aatgtcgatc actccacgat
     1921 ttaccgctgg gttcagcgtt atgcgcctga aatggaaaaa cggctgcgct ggtactggcg
     1981 taacccttcc gatctttgcc cgtggcacat ggatgaaacc tacgtgaagg tcaatggccg
     2041 ctgggcgtat ctgtaccggg ccgtcgacag ccggggccgc actgtcgatt tttatctctc
     2101 cccccgtcgt aacagcaaag ctgcataccg gtttctgggt aaaatcctca acaacgtgaa
     2161 gaagtggcag atcccgcgat tcatcaacac ggataaagcg cccgcctatg gtcgcgcgct
     2221 tgctctgctc aaacgcgaag gccggtgccc gtctgacgtt gaacaccgac agattaagta
     2281 ccggaacaac gtgattgaat gcgatcatgg caaactgaaa cggataatcg gcgccacgct
     2341 gggatttaaa tccatgaaga cggcttacgc caccatcaaa ggtattgagg tgatgcgtgc
     2401 actacgcaaa ggccaggcct cagcatttta ttatggtgat cccctgggcg aaatgcgcct
     2461 ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct
     2521 aactttgaac agtgcccgag cccctgcagg caggagagcg cggtcagata gtggctgtgc
     2581 aagtggacga tcgccttgca ggccgggttg tgcaaataga ccgcccggtg aaaagtgacc
     2641 tctttcgacg gtttgtcgcc ggagatccat tccccttgca gcgtcaccac cgacaaccgc
     2701 tgagcctgca gttcgccgag gcaggcgccg gtcggcgtcg ccagcaggtt gccgtccggc
     2761 agcagcagcg acagattgcc agcggagccg gtggcatagc cgcggctaaa caacgaggcg
     2821 ccaatctgca ccatttcctc tcgcagttgt tgctcagtca tgacgaaact cctgctgggc
     2881 acgggcaaag aactggatat cgccgaagtt acctgacttc agcgccaggg agagcggctg
     2941 gcgggtgtca cgcacccagg gcacgccagg ggagatggtc ggcccaatat ggaacgcctc
     3001 aacccccagg gtctgcgcca caatgctcga cgtttcccct ccggccacaa taaagcgggt
     3061 aaatccgttc gccttcaggg cggcggcaag agcggcaaac agctgttcca cccgttcgct
     3121 gctggccttg tcgccatact gcgcctggat ccgctgcagc gtttgcggct cggtggtggc
     3181 atagatcatc ggcgccagcg gcgcatcgcg ctgcgcgtcc acccagtcag tcagcgtcct
     3241 gacgtagctc tccagatcgg taaagcaggc gcttaagtcg acggcgcggg cgggggcctg
     3301 ttgacgatag gccgccacct ggctgttggt catcaccgag caggagcccg agagcaccac
     3361 cgccggaccg gccagcggca tgccggcctg agcgctttcc cccctggcgc catggcgctg
     3421 cgcccagtcg cgggcgaggc cgatggcgag gccggaaccg ccggagacca gcttcatctc
     3481 ccgcagcgcc acgccctggg tgagcagatc ctgttcgctg agggcgtcga gcaccacata
     3541 gcgcaccgcc ggatcgttga ctgccgccag cgcggcggcg accgggcgac aatcggccag
     3601 gcaatcagcg cggcttttcc gcgcccttgg cgctcaatta agcggcccag gtgcgcatcc
     3661 tccatcggcg tcaccggatg gtggcgcatc ccggactcat tcagcagttg ctcgccgacg
     3721 aacagatatc cctgatagac cgtgcggccg ttaaccggca gcgccgggga aatcaccgtc
     3781 cgcgtctcac ccagctcggc cagcagggca tccagcaccg ggccaatgtt gccctgcgcg
     3841 gtgctgtcga aagtggaaca gtacttgaaa taaaactgct gacagccctg ggcctgcagc
     3901 cagcgcaggg ccgccagcga ctggctgacg gccatttccg ccgggcagga gcgggttttc
     3961 aggctgatga ccaccgcctc gctggtcagc ggaatatcgc gggtcggcac gccattcagt
     4021 tgcaccgtcg gcatgccgtt gcgcacgagg aagctggcaa tatccgtggc gccggtgaag
     4081 tcatcggcaa tgacaccaag ctgcatgttg tctctcctca ctgtttatgg cccggcaggg
     4141 tgatgccgtt gaaaatcttg atcaccgcgc tgtcatcttc ccgaccgaat ccggcattac
     4201 tggcggaggt gaacatattc agcgcggtgg tagcgagcgg cagcgggaag gtcagcgccc
     4261 gggcagtgtc attcaccagc ccgagatctt tgacaaaaat atcgacagcg gattttggcg
     4321 agtaatcgcc atccaggacg tgctgcatgc gattctcaaa catccaggaa ttacccgccg
     4381 cgtgggtgac cacgtcatac atcgtctcca gtgggatccc ggcgcgggcg gcaagcgcca
     4441 tcgcttcggc ggcaacggcg atgtgcaccc cggccagcag ctgatggata atttttaccg
     4501 tcgagccaag accaatgtcg ctcccgatgc ggtagacttt gccggccacg gcgtccagca
     4561 ccggcgcgag tcgggcaaag gcggcatcgc tcccggaggc catcaccgtc atgtcgccgg
     4621 cggccgcttt cacggcgccg cccgataccg gcgcgtcgag catcaatagc tggtactccg
     4681 ccagcgcctc ggcaatggcc tgagcatcgg cggaggcgat ggtggacgac accatcacga
     4741 cggtgcccgg cttcagatgg gcggcgaggc cgctctcgcc gaacaggatc ccccgcacct
     4801 gggcggcatt gaccaccagc agcacaactg catccagttc cgcggcgaac ggcaccgcgc
     4861 tggggcccgc gcctttggcg cccgccgcca gcagtgcgcg acagttgtcg ggattgatgt
     4921 caacgcccca ggtgttcagg cccgcctgca ggcaggcgcg ggcggcgccc atgcccattg
     4981 aacccagtcc aatcacgcag acgttagtgt gtgcagccat gctggtctcc ttgtgaacat
     5041 tagttaatta atgtgattta atgatagaat aaagcgttat catgtgaatt tatgtgagag
     5101 ttatcacgaa taacccacat gaatataaaa aattcacagg cggaaagagg ctgcacagcg
     5161 gcgattcttg ccctaaaata gcgtaaaaaa cagcggcaaa gcgcgataac aacaccgggt
     5221 gtcgccaggg ccgtagcctg ggtggtgacg cctggtgtga aaaaatggta aggggagagc
     5281 g
//
