LOCUS       CP142572               16185 bp    DNA     circular BCT 19-JAN-2024
DEFINITION  Escherichia coli strain ExPEC_A251 plasmid pA251_p1, complete
            sequence.
ACCESSION   CP142572
VERSION     CP142572.1
DBLINK      BioProject: PRJNA1044843
            BioSample: SAMN38414390
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16185)
  AUTHORS   Li,X., Zheng,J. and Tan,C.
  TITLE     Diversity of population structure and antimicrobial resistance of
            swine extraintestinal pathogenic Escherichia coli from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16185)
  AUTHORS   Li,X., Zheng,J. and Tan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2023) National Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, Shizishan Road,
            Hongshan District, Wuhan, Hubei 430070, China
REFERENCE   3  (bases 1 to 16185)
  AUTHORS   Li,X., Zheng,J. and Tan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-DEC-2023) National Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, Shizishan Road,
            Hongshan District, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/16/2024 11:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,826
            CDSs (total)                      :: 4,706
            Genes (coding)                    :: 4,487
            CDSs (with protein)               :: 4,487
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 81 of 219
            Pseudo Genes (incomplete)         :: 136 of 219
            Pseudo Genes (internal stop)      :: 33 of 219
            Pseudo Genes (multiple problems)  :: 32 of 219
            Pseudo Genes (short protein)      :: 2 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..16185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ExPEC_A251"
                     /isolation_source="lung"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pA251_p1"
                     /geo_loc_name="China:Hubei"
                     /collection_date="2014"
     gene            complement(join(15742..16185,1..348))
                     /locus_tag="SNB03_24065"
                     /pseudo=""
     CDS             complement(join(15742..16185,1..348))
                     /locus_tag="SNB03_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(727..2928)
                     /gene="iutA"
                     /locus_tag="SNB03_24070"
     CDS             complement(727..2928)
                     /gene="iutA"
                     /locus_tag="SNB03_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="WRY17244.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTVA
                     EMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVLVD
                     GVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETMMEF
                     EAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDNTQTG
                     LQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTSTPFVSN
                     GLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQVTAFSS
                     SQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGGLNNKKIY
                     TTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQRQIAAGKA
                     TSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYGRGIYGAAVN
                     GHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKSVVANKDLTIS
                     VVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDVKTASPSKATAY
                     IGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTLSFSIENLFDRDY
                     TTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
                     /name="iutA"
     gene            complement(2954..4287)
                     /gene="iucD"
                     /locus_tag="SNB03_24075"
                     /pseudo=""
     ARG             complement(3014..4287)
                     /name="iucD"
                     /gene="iucD"
                     /gene_name="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="97.65"
                     /coverage="95.51"
     gene            complement(4284..6026)
                     /gene="iucC"
                     /locus_tag="SNB03_24080"
     ARG             complement(4288..6026)
                     /name="iucC"
                     /gene="iucC"
                     /gene_name="NIS family aerobactin synthetase IucC"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="97.59"
                     /coverage="100.0"
     gene            complement(6026..6973)
                     /gene="iucB"
                     /locus_tag="SNB03_24085"
     ARG             complement(6030..6973)
                     /name="iucB"
                     /gene="iucB"
                     /gene_name="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="96.19"
                     /coverage="100.0"
     gene            complement(6974..8698)
                     /gene="iucA"
                     /locus_tag="SNB03_24090"
     ARG             complement(6978..8698)
                     /name="iucA"
                     /gene="iucA"
                     /gene_name="aerobactin synthase IucA"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="95.31"
                     /coverage="100.0"
     gene            8834..10027
                     /locus_tag="SNB03_24095"
     CDS             8834..10027
                     /locus_tag="SNB03_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0022857 - transmembrane transporter activity [Evidence
                     IEA]; GO_process: GO:0055085 - transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WRY17240.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTNL
                     SLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLMAT
                     GAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSCGRL
                     FGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDCLPYL
                     LCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVVLRPQR
                     LTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLNDKLADGA
                     GAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWHYRTIK"
                     /name="MFS transporter"
     gene            complement(10407..10787)
                     /gene="crcB"
                     /locus_tag="SNB03_24100"
     CDS             complement(10407..10787)
                     /gene="crcB"
                     /locus_tag="SNB03_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="WRY17241.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLAG
                     LIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTSVL
                     VHVIGSLIMTALGFFIITILFA"
                     /name="crcB"
     gene            10967..11670
                     /locus_tag="SNB03_24105"
                     /pseudo=""
     CDS             10967..11670
                     /locus_tag="SNB03_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]; GO_process: GO:0006313 - DNA transposition [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            11812..12552
                     /locus_tag="SNB03_24110"
     CDS             11812..12552
                     /locus_tag="SNB03_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]; GO_process:
                     GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WRY17242.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAYL
                     LAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEKVR
                     ARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRRA
                     EADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFALDMAA
                     TLAVPFTGDGRDAAEILRTLPPLR"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(12837..13814)
                     /locus_tag="SNB03_24115"
     CDS             complement(12837..13814)
                     /locus_tag="SNB03_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WRY17243.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNTL
                     SRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVELP
                     FVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSAYYD
                     TERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPAPISL
                     ARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPRLKAPN
                     DESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
                     /name="RepB family plasmid replication initiator protein"
     gene            14560..14883
                     /locus_tag="SNB03_24120"
                     /pseudo=""
     CDS             14560..14883
                     /locus_tag="SNB03_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            14885..14989
                     /locus_tag="SNB03_24125"
                     /pseudo=""
     CDS             14885..14989
                     /locus_tag="SNB03_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /name="AAA family ATPase"
     gene            15050..15562
                     /locus_tag="SNB03_24130"
                     /pseudo=""
     CDS             15050..15562
                     /locus_tag="SNB03_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /name="DUF4113 domain-containing protein"
     BGC             1..16185
                     /name="NI-siderophore"
                     /gene="NI-siderophore"
                     /tool="antismash"
     Typing          complement(12838..13814)
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.569"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acccgacata gccccactgt tcgtccattt ccgcgcagac gatgacgtca ctgcccggct
       61 gtatgcgcga ggttaccgac tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag
      121 gccaacgccc ataatgcggg cagttacccg gcatccaacg ccattcatgg ccatatcaat
      181 gattttctgg tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt
      241 acggcagtga gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac
      301 cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca
      361 aaaacaccat catacactaa atcagtaagt tggcagcatc accccgcagc cgtagatatt
      421 ctagccacgg tggtaacgag tatccggaag agattccact tacactcttt caatcctatt
      481 gcaaaagctg atttactcaa caggattaaa agagggagtt tgatacaatt tatttcactt
      541 tttgttagaa tactaattct tttcttgtat ttttttgagg aaatgagcac ggaacacgac
      601 ataatctgta gtcagtaaag agtctatcct ccctgactac agattatgtc gtgtattata
      661 tgatatcaac ttggctgtca gcggccatgt tgatatcagc gtacctttgt tgtaaaggaa
      721 taccggtcag aacagcacag agtagttcag accaaaggtt cggcccctgc ctttgtagtc
      781 gtacagtgac gctgggccgt aacccgggct gtagtacagt ggtgcacgct gcccccagac
      841 agtggtgtag tcacggtcga agaggttttc aatgctgaag ctgagtgtac ccaccggaag
      901 ctgataactg ccgagcagat ccacggtggt atagccatcg accttgtagc cctgcgcgtc
      961 gctcacgtca aaggaggtgg tgctctgcac gcgcagactc cacgggtccg gtgcccagcc
     1021 aatgtaggct gtcgcttttg atgggcttgc tgtcttcaca tcgtatttct gccaggtacc
     1081 gttcactttc gactcagttt tcagcacgtt gaagttcact ccggtactcc agtcagtatc
     1141 aggaatcagg tagtccaccg caccttccac gccgtaaata cggcgtttgt cgtccaccac
     1201 gctgatggtc agatctttat tcgccaccac gctcttatca gaaatcgaat agtaggccgc
     1261 gatttgggta cgcagattat tgccagtaaa gcgccagccc agctcataag aatcgacttt
     1321 cacgccttcc agcttgctgt cgctgacgtt cacactcttt gttagaggaa gatggccgtt
     1381 cactgcagca ccatagatgc cgcgaccata gtatttaccc gggtccggca gctccacgcc
     1441 ctgggagaag ttgagccatg cctgctggcg ttcggtgatg tgcatcagca gaccggcgtt
     1501 gaacaggaag ttgtcgtaat cgactgagcc gccaggaatg gcgtcggcgg atgtagcctt
     1561 cccggcggca atctgccgtt gctgcgcgta gccgatgaaa tcatcaatct tgttttcagt
     1621 gtactgatag cgtacgccac cgttgagggt aaagagatta ttgatgtcat agcctgattg
     1681 caggaaggcc gccaggttgg tgatgtcata cgacggatag cgcccggtgg tgtaaatctt
     1741 cttgttgttc agccctccgg aagcgcttgc ctgagccagg tcgaagaaca tctggttgga
     1801 ggtaaagcgc tcatgatcag catccagccc ccaggtgatt tgccagccgt ccatcggttt
     1861 gctgttcaga gtcagtttca tgccgtactg gtcggtgtcc tgctgtgacg aagagaaagc
     1921 cgtcacctgt ttgttcgcat ttaccgtcgg gaacgggtag aatcgcaacg actcatcgcg
     1981 gtagtaaacc tgaccgacca gctcctgtcc cagaaaagcg ctgtcagagt actgcaggct
     2041 gatcaaatgc cgctcagtgc cgggaatacg gtcggaattc agcccgttac tgacgaatgg
     2101 cgtgctggtc cctctgatgg cagagaagcc tttcccgaga ttaagcccgt aatcgtcgtc
     2161 gccctggctt ttatagtact gtgtgatcaa ctgaagctgc cgggattcat cgatgttcag
     2221 cgtaccagtt cccatgatgt ccagccgatc ggagtactgc aggccggtct gggtgttatc
     2281 aagcaaggtg gcatcgccgt taccgtcaaa ccagccgcca aatttctgat atgccacgga
     2341 aagacgtccg gagatatgct catttccgcc ggagacagct ccggcaatgc gttcatcgtg
     2401 atctttactg ctgctaaagc cacttttggt gccagcctca aactccatca tggtttccgg
     2461 ctggcctttt ttggtcacga tgttgatcag gccaccggta ctgccgccgc cgtacaggga
     2521 cgtcgcaccg gagatcactt caatatggtc gatattaaaa ggatctatag agtccagttg
     2581 tcggctgtcg gtacgtgaag agttgagacg cacgccgtca accagcacga ccagcgggcg
     2641 gccacgcaca ttcataccgt agttggtgcg gctccggctg ctgacgtcaa ggccagggat
     2701 cagctgagcc agtgcgtctt taagctcttt gccgccctga atctgctgtt ccagttcggc
     2761 gttttcgata acccaggtgg tttgcgccat ctccgctacg gtgcgattgc tgcggttggc
     2821 agacaccacg aacgtttcat catcggtttg ttgagcgact gctggcgcca tcatggtaag
     2881 aagcagtggg ttgagagccc aaagcgtata ctttttgctt atcatcatta ttcctccata
     2941 cgggcggaaa aaatcagccc aaagatgctg tgtagcgagt taaagaagca gcctccggct
     3001 gcgttttccc taggtgccgc tgcgccactg aatcagggcg ggcggcatac tgagatcgaa
     3061 taaatcacgt cccattacgc gattaagaat acgtgcagat ctccatgcca tcaggctgag
     3121 ctggggttcg gcgatgccat gggtttgcat actggcgttg accacgaaga tgttgttctc
     3181 tttcgggcca ctccattcca gagtgaagtc atcacgcact ttaaaggtgt tcttatcgtg
     3241 catggtgatc aggggcatca gtgagggaag tatttgtggc aacgcagaac ggtaacctgt
     3301 ggcgaaaatc accacatcac tttccaggct ctccctgccc tgatccagat gatgctccat
     3361 cagcaacttc cagccaggac cactactttc cagagttgtt accgagcggc tgggtagcag
     3421 acggatattt cttggttttc tcagaacttc aaaacggtgg tacaactcac gataaatggt
     3481 cagtaaagaa tcggcagtga tgccatccga tgtcattttc tgctcatcca gtaactgatg
     3541 gcgaatatct tcctccagtc cggagaagcc tgaaatatat tcaggtgtaa aatactcatc
     3601 agcaaaagca gcctcatcca gtgcgttaaa attattacgc cgggagaccc agtttatttc
     3661 cgccgcttct ccccattccc cgcgtaatgc attaaggaac aggtctgcac cactctgtcc
     3721 tccaccaacc acggttatcc gttttccact aaggtccggc cgacgaagat tcatttcact
     3781 ggcatggaaa caggattgtg tcatatgctt cacacagggt ggtaaataag gttgttttcc
     3841 tgtaccaagg cagatattgc gggcaaaata ttctccctgg ctggtttgca ccagaaacaa
     3901 tcgacgtttt ttatcgaaat caatgttttc aacggtatga ctgaaataca ggttattcat
     3961 atcttcagca gcccagcgga ggtagtcaga aaactcttca cgggatactg tacgtagtct
     4021 gcttgtaagg aagcgataga actttttgtg cttcaccaga tagttaacaa aactgtaggg
     4081 atttgtaggt gcaacagcac tgaccagatc tttcagaaag acggtctgca tatgacaatc
     4141 cggtaccagc atacccggat gccaggaaaa atgaggatgt tcatcaaaga agagacagtc
     4201 cagttcttcg atctgatgtg acaacgcagc aatgctgaga ttaaatggcc ctgtccctac
     4261 accaataaaa tcgacacttt ttttcatgat tcatattcct gagttaccag ccacagcgga
     4321 ttttgcagat cctcaaggta attcggcagc atgcggctgc cgccatccag atcaggccag
     4381 gtcagtttta ccgggttcag caccacgcga atgatttgtg gcctgaaaag tgagaaaagc
     4441 gcaaaacgct ctgacatttg tggatgtttt ttcatgtaat cactcaacac tgctgccagc
     4501 agttgataaa atcgcctttc aggtacgcca agacgaacca tcagtggcga aataaaacgc
     4561 agtactgtca cgaagtgacc cgtctgcaaa tcatggatta agtagtccgc actcaggcgg
     4621 gatgtaacat cacgaacctc ctgaggcaaa gagtccattt cggggaactc ttctttcacc
     4681 agtcgcatat cgccctggaa gtctttcagc agaacacgct gtggaacccc ctctttcatg
     4741 gcgagagtta tattttgtcc atgtgcaata agcgcgacac cgtagcggca aagcaggtga
     4801 tacagaggaa ccacgaccac ccggaacagt tgcgtaagcc aggtttcagc gtccagcccg
     4861 gagcggtcta tatatgcgcc tgccagcggc tgattgtttt cgtcgcactc catcagtgtt
     4921 gccatcagaa ccggactttc atccggtttc agccagcggc acggattctc ccgccagata
     4981 acaccaagca tttcctggta gcgataggga gcccgggcaa gcgcggcata gccttcatgg
     5041 gagacatagc ctgcagccgg ttcaccaagt atcactgcgc cgctttgcac tagggtggcg
     5101 tcggtcgcaa aaacctgttg tagccagcgt gaagccagtg gtccggcagc gatgtatctg
     5161 ccagggatcc cccggtagca tgaggtgttg tagatggtca gcggcagctt gatatccagc
     5221 ccgccccggc gactggcgtt ggtcagggta cgcagcgact gctgggcgag ccactggtcg
     5281 ccaaactcgc cgagagacac catcctgcct tcggcaaaat cagcgatgaa gtcggtagcg
     5341 attttttgct gccactgcca cggatgtacc ggcagcggca gccagttatg atccagtccg
     5401 ttttcctgcc agacctgact gaagcgggca aactcctgcg gatccattgc ggccgtcaac
     5461 aactgatgaa tatccatctc gttatcacag cgccagatca tatgttcacg ttttaccgcc
     5521 agccagtgca gtctgaaggt gttggcatac tctggcgcat atcgttccag cgcctcttta
     5581 ccccagccac ggcgaccttt attaaaaacg aatttaggat gaccgctcag caggcattgc
     5641 aggcggtcgg cattaagatt aatcaggtca ctggcgctca gcccgcgacg ggctttcagt
     5701 agttgcaggt cgcccagcag cgtggcatac aaatcctgca tatgctcagc aacggttgca
     5761 tcgctcattg acagtacctg ctttagctgc atcagcagcg tctgagccag tactggctcg
     5821 tccgcgcagc gcagagtttg agcatcaatc cagagccagc cccagatacc acgttcagcg
     5881 atgaagcgcc attgtgctcc cggcaggtta atgcagtagc ggtcatcgcc ctgagattcc
     5941 gcgtggaaaa cctgctcata ctccagctca gacaacattt ttgccaccag gcggcggttg
     6001 accaaatccc agtctttgtg attcattaca gaccgacctc cgtgaagaag tgatgacgat
     6061 ccgccatcac catgcgcgag cgtttatgtg ggaagtcgaa ctctttaatt gtccggtatc
     6121 ccgcaggctc aagatggcgg aacaggcgct ggttatcggt gcgtggctcc agtactgtgc
     6181 gctgcgtacg tggctcattc agcagcaggt aatgtgtcac cccgcgcagc cagctttgca
     6241 catagtgggc tccgcgccat tgctgttcac caaccagcag atgcaggcca cggtcaaagg
     6301 gctgccacga atagtggcga ccaatgcggt cttccgccgc ccagtagatt tcgaaataac
     6361 tgaacggccg atcgtcgaaa caaccaatca gcgggaatgc atgtttactg gtgagctggc
     6421 gttccagata ggcgatctgt acctccagtg agccactttg ctcccagaaa tactcaacgc
     6481 gcggatcgtt catccagcga gtgaaacgtt ctgcatcaga aacgggatcg gcaatgcgga
     6541 aactcagcat cctgcgaatg cgtggatcgt aacgacgata aacttcaccc tgcggacggg
     6601 gggggcgctg cgggaaataa atctcacgct ctgcatcaaa aaccatttca ccggaggccc
     6661 ggtttgccgg agaactcagc cataacggta actgccagaa agcggaacga tggataatgt
     6721 cgctttgtac ctgtccgaaa agcgtcagcg cctgtggctc ctcacaccat tcggaccagg
     6781 gaagaaccac tgctgaaagt tgtggtgcgg cgataaatat ctgatccagc gcgtcgcaca
     6841 gccaccctgt tggcagctcc ccgggccagt gcaacaccgc gctattgtcc agcccccagc
     6901 caagattcag aggcttgtcg agtttttcac agcgcagtcc atatccgctg tgaacaatgt
     6961 ttgccccaga cattcagacc tcctgagcct gcagcgggtt agcgaaatca aaatagatca
     7021 ccgaagggtc aacgatggtg ttctcgttat gatcgttcag ataacagaaa aagttacctt
     7081 ttacgttcca gtacggactt tccagaacat agttgagaca ggttttatga gtcacctggt
     7141 cacgcacttc agccagcaat gttcgtacac gagccatcag attcgcttcg ctgtccagcc
     7201 cggcagcacc cagcgcagca gttacggcaa aagtggagtt aaccagcagg taataaggga
     7261 aatagcgcag caactgctca cgggtgaaaa tattttccgc ctgcgcctcg tcaatggtat
     7321 cgagccactc tgtcgcgtga ggcataaacg cgctgccctg gcagtcacgg taaataaatc
     7381 cgaccggcag atccccaagc atctgcacaa gaatattttg ctgatgcgcc agcagcacca
     7441 ggccgtaatc cgcttcagcc gtaaacagag gcttcagcac ctgctgacag taagcatcga
     7501 cccatgcgtg ggcggcctgt tgcacagtga taccgaggcg atcgctcagg cgttttaccg
     7561 ccgaaaccag cagcgaatca ccgccatccg gtgcggcctg agtcagggaa accagaacgt
     7621 tggtctggct ttgcggctgc tccagcagca ggttttcacg cagggaaaac aaactttcct
     7681 gcatgatgtt gccgttaaga tcgcgcagcc cggcccagcc gtcctcctgc attacccgga
     7741 aagtagggaa gcgggcctgt agcatctgcc agccgtcggt ttgcgccaga cgtgccaggc
     7801 gcattcctcg ctccacctct ttcacagaca gagtacggac ggagttggtc agccgaacgc
     7861 tcagggagaa cttgatcata tcgcggctgg tagcacagta gagggagcgg gaagaggtgg
     7921 tcggcagcca cgacgtgccg gcctcgccta agtctctgat aagtccttta gcaaaaagtg
     7981 cctggcacca cacttgctgc aaaagatatt ctccctgcca cgggtgcagc gggaacagcc
     8041 attgattgtc acttaattcg ttgagtaact ggggcgcatt ttctgcggca aatcgcgtca
     8101 accgctgttg aaggttaaga tgcagacttt caccagcgat ttgcgtttta tccaccgaaa
     8161 accaccgcag cgggaagtga ggtgccatgt caggcaggta tcgttcagct tcctggcggt
     8221 taaacggttc atgagactta ggtgcagggt ggaaagcgtg tcctgtcagc agtgcctgct
     8281 cagcctcgcc aaaattcaac gctttttcac gcaggatggc ccagtcatga cgggcatcaa
     8341 ttgtctgttg cgtatgggcg tagctttcca gcacgcgttg gtggaaagtt tcgcagaggg
     8401 gaactggcag aaaaagttga tgccggagct tatcaataat aagccgggag agagtggtaa
     8461 agtcgacagg ataactgcct gatgccgtga ccagatgagc aggaaatgca aagcggtgat
     8521 gctgggttgg ggagaaataa gccactctga aatggagcga cttctgttcg tccagcggga
     8581 taatcaattc gtctggcggg tattccgcca gttgccagtc tttggtttca cgaatcaagg
     8641 cattcaggaa gcactgcgca gccacatctg tggcggattt ttcagagggc aggatcataa
     8701 tcaaacagct cctacacagt aaaataataa caattatgtc aataatgatt ctcattatca
     8761 atgagaaatg ttattgtcag aaaaattaat tttacaaata aacacaatta atacatggtg
     8821 ttgaaacatt acaatgtcat ctaagatcga agatacgccc caaaaaaccc tatcctgctg
     8881 gccactggcg ttcagtgccg gtcttctcgg tatcggacag aacggtctgc tggttgtact
     8941 ccctgttctg gtcatacaga caaatctgag tctgtctgta tgggctgccc tgctgatgct
     9001 gggctcaatg ctgtttctgc catcttcccc atggtgggga aagcaaattt cccttactgg
     9061 cagtaagact gtggtgctgt gggctctggg aggatatggc gtaagcttta ccctgcttgg
     9121 gctgggaagc gtgctgatgg ctaccggtgc cgtaacaaca gcggtggggt tgggaatatt
     9181 aatcatcgcc cggatcgtct acggtctgac cgtgtcagca atggtgccag cctgtcaggt
     9241 ctgggcattg cagagagcgg gagaagggaa tcgcatggcc gctctggcaa ccatcagctc
     9301 cggcctgagc tgcggcaggc tattcgggcc gctgtgcgcg gcggcaatgt tggtcattca
     9361 ccctctggcg ccagtgtgga tgctgatggc agctccggtg tgggctgtgg tgatgcttct
     9421 gcggttgccc ggcacaccac cacagcccac accggagcgc aagagcgtca gcctgaagcg
     9481 ggattgcctg ccttatctgc tttgcgcaat gttactggct gcggcaatga gcatgatgca
     9541 gcttggactt tcgccagccc ttactcgcca gttcgatact gataccacca ctattagcca
     9601 acaggtggcg tggttgttgg ggctgtccgc aatagctgcg cttatcgcgc agttcgtggt
     9661 actccgtcca cagcgcctga ctccagtggc tctgctcctg agtgccgggg tgttgatgag
     9721 tagtggtctg gctatcatgc tcactgaaca gctatggttg ttttacctgg gctgtgcagt
     9781 gctgtcattt ggggccgctc tggcaacacc cgcttatcaa cttttactga atgataagct
     9841 ggccgacggc gcaggcgcgg gctggcttgc ggccagtcac acacttggct atgggctgtg
     9901 cgcattgttg gtgccactgg cgtcgaaaac aggtgtctca atcgcgctga ttgtgacggc
     9961 attatttgcc gctgtattat ttaccatagt gtctgcatgt atttggcatt accgtacaat
    10021 caagtaaggc tatgatgttg tactcacagc tggcataacg tcctgtgcaa tttcgctgtc
    10081 gaaaccgcct gtgctgaccg cccagtgcac tcacctgaag cacatcgcag catgccacca
    10141 ggatgccaga tcgtcttctg aaatgggtgg atacattggc aacgttaacc gcaacaattt
    10201 ttcagagaga attttggggg gtgagggcca gtgatgtaaa aaatacgcca agaggttaat
    10261 tcattgttta tactgatatg tcaggtaatg cgtttgcagt cattacagtc gtcatggaga
    10321 ctgtaatgac tgcaaacgca taccatggag cgaaattcta tcctaagcgt cacattagtt
    10381 gataaacctg taacgttaat gaattctcat gcgaaaagta tggttatgat aaaaaagcct
    10441 aaagctgtca tgatgaggga gccaatgacg tgaacaagta cagacgtcaa agcccatata
    10501 taatttcccg cctggagtaa tgcgaatact tcaacagaaa atgtagagaa agtactcaga
    10561 cctccacaaa gtcccgttgt tatcattaat ttccaaaaag gatcaaggtg tggttgtcgt
    10621 aaaaagtacg ccaacgcagt accaataatt aacccggcaa ggaggttcac caccagtgta
    10681 cctggaggta gatttggaaa aagactgtta aagcgtgtgg acagtaacca gcgcagagta
    10741 catccaacag aaccgccgat aatgacggca aaaagtgatt taatcatgta cccctcataa
    10801 aataattttt tatcgtaaca atatcagaat atgttctcat ttgactgata ttgttaattt
    10861 atatcctttg actacatgca agataaggga aggttctatt tatggcactg ttgcaaatag
    10921 tcggtggtga taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa
    10981 aggccggcat tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg
    11041 catcagttac cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc
    11101 cacgatttac cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta
    11161 ctggcgtaac ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa
    11221 tggccgctgg gcgtatctgt accgggccgt cgacagccgg ggccgcactg tcgattttta
    11281 tctctcctcc cgtcgtaaca gcaaagctgc ataccggttt ctgggtaaaa tcctcaacaa
    11341 cgtgaagaag tggcagatcc gcgattcatc aacacggata aagcgcccgc ctatggtcgc
    11401 gcgcttgctc tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt
    11461 aagtaccgga acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc
    11521 acgctgggat ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg
    11581 cgtgcactac gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg
    11641 cgcctggtaa gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa ggctgcctca
    11701 tcgctaactt tgcaacagtg ccctgcgatt tattgtcgtg gaaggatccg ggcgttcagg
    11761 tcagaatacc tgtatccata aacagtgcgt gtgctacgtg aaaaataact catgaacaat
    11821 gtcattcccc tgcagaattc acctgaacgc gtctccctgt tacccattgc gccgggggtg
    11881 gattttgcaa cagcgctctc cctgagaaga atggccactt ccacgggggc cacaccggcc
    11941 tacctgctgg ccccggaagt gagtgccctt ctcttctata tgccggatca gcgtcaccat
    12001 atgctgttcg ccaccctctg gaataccgga atgcgtattg gcgaagcccg gatgctgacg
    12061 ccggaatcat ttgacctgaa tggagtaaga ccgtttgtgc ggatcctgtc cgaaaaagtg
    12121 cgtgcgcgac gcggacgccc gccaaaagat gaagtgcgcc tggttccgct gacagatata
    12181 agctatgtca ggcagatgga aagctggatg atcaccaccc ggccccgtcg tcgtgaacca
    12241 ttatgggccg tgaccgacga aaccatgcgc aactggctga agcaggctgt cagacgggcc
    12301 gaagctgacg gagtacactt ttcgattccg gtcacaccac acaccttccg gcacagctat
    12361 atcatgcaca tgctctatca ccgccagccc cggaaagtca tccaggcact ggttggtcac
    12421 agggatccac gttcgatgga ggtttatacc agagtgtttg cactggatat ggctgccacg
    12481 ctggcagtgc ctttcacagg tgacgggcgg gatgctgcgg agatcctgcg tacactgcct
    12541 cccctgaggt gacggcatac gcgcttcctt atataagctg tggtcagcag aacagacata
    12601 acataagctg gagcatgtat ataagctgta gtgagtaagt catgttttat gaagggagca
    12661 atgcctcagc atcaggttac gggatcactt acgtaaggga caggcagatg gcagctcagc
    12721 tacagacagc actgcaggaa acagaatata aactgcagtg agcagaccac tcactgcacc
    12781 ggttaatata agctgtagtc agtaaaggag cgctcttcac tgactacagt ttatgttcag
    12841 cgggatttga agagtttttc caggtcatcc agcgtgatgc ccagtttctc aagcagggca
    12901 agcttctccg ccatctccgg actgactttt tccgcaggtg aaggttgcaa cggattttcc
    12961 ttactctcgt cattcggcgc ttttaaccgg ggacgccggt agtgaataca gaagaatttt
    13021 gtccgccccc gctggatctc cgtgtaatca agatatccaa tctcgcgcaa ctgctccatt
    13081 gcccgtctga ccgtctggtt ctgggaaaat acaggagact tcagattgag gcgtgcacgc
    13141 agccgcgcca gcgatatcgg tgccggatcc cggggcaggc tctctataaa ggtgtagagt
    13201 gcctgggcgg actcccgtcg cttcagggca ttaatcgcct taagctggag aaggactttt
    13261 ctgtcaaact ggtacagttc aaacaggcgg ggatcggcct gtaactgcac aatatcccgc
    13321 tcagtatcgt agtaagctga ctgtaccaga tgggtgatat attcccgggt gtgcttctca
    13381 tcggtacggg aaaacgaaat cacggtaccg gcaatgcgct tcagggaagg gctgatgcgc
    13441 tcacgcagcc tgcgggatga ctggcttgaa ggtataccac acagttttgc aaactcaaca
    13501 aaaggcagtt caactttgtc accaatcacg ttatggcggg caaaggaatg aatgatcccc
    13561 acccaggtct tgaaatcatt atccatatcc aggcgggggc cggtgatctc aaccttatcg
    13621 aatccctcag cacgggccag ggaaagacgt gtcagctctt ccgtggcatc agtacgtgac
    13681 agtgtatttt ttttactgtt cttcagtgat ttaagggtcg gtacaaaaac gcccagacgc
    13741 atcagcgcca caggttgtac ggtgttgtta ttgtttggtg tcagtgtcac cagctcaccg
    13801 gaggacttat ccacctcgcc gaacagtttt ttgatgtctg aattttcgtt ttccagaaag
    13861 cctccagcct gtggacagta aaagaataaa cagaaaatcg tgtgtcagaa ctccgctaac
    13921 tgactgaaga aaaacctgca gcctgtattt aacaactgag tctggcatac tgaccatagt
    13981 ttatgtcatt aacttacttc agtatatgga ttactaaata cagcttatca ttagcatgtt
    14041 acagtttatc agtaacatgc cacagcttat gtgatcgtgg tcccggccca tgagtgacaa
    14101 ggtttgcagc gatatgggga tctttaatgg atcctttgtt gatcattcat ggatctgttt
    14161 actggatcgg gcctccggat atgtggataa atcattccag aaggacagcg cacacaaccg
    14221 ttttatgaaa gggcttcagg gcgtacctgc tccctgtaac tacagataca ttgcgcttcc
    14281 tcgccggttc gcgggcttcg cccggtcacc ggtctgcggc gagcactgtt ttttctgatg
    14341 tctgtcttta caggtaattt ttgacggaat ctgccgtact tcctgagcgt acggtcatat
    14401 ccgaagtaca cttcatatgt gtgtttcaat gccagggatt gtgtatacaa actacaggtg
    14461 tcagcgccag tgatataaga cggtaattca ccatttggat tgtccgctcc acccaacatg
    14521 ttgtttcctt aaggttctca caccagaaag gacatcaaca tgctgagcag agaggacttt
    14581 tacatgataa agcaaatgcg ccagcagggc gcgtacattg tcgatattgc ggctcagatt
    14641 ggttgctctg aacggacggt cagacgctac ctcaaatacc ctgaaccgcc agccagaaag
    14701 acccgccaca aaatggttaa gctgaaaccg tttatggatt acatcgacat gcgcctggca
    14761 gagaatgtct ggaatagcga ggttatcttc gcggagatta aggcaatggg ttatacgggc
    14821 ggacgttcca tgctgcgtta ctacatccag cccaaacgta aaatgcgtcc gtcaaaaaga
    14881 acaaggagag agttaccggt taaaagagaa acgtaaagct ggagtgctga ccaaaaacac
    14941 aacgccaatc agtgatgatg aaatggtgca aagcggacag catcagtaac gaaagtattt
    15001 agcgggcatg aaaatggcaa ataacggtca aacatcgtgg cgttgacata gtcgcagttt
    15061 tggtgaacga atcacagaca aagatgccat gcaccaggct gttgttcagt atgcagagcg
    15121 ggccgcagag aaactacgtg gggagcgtca gtattgccgg caggtgacga catttgtacg
    15181 gacatccccc tgaaccctgt tacagcaatg ccgctgtgga aaagctttca ttgcccacac
    15241 aggacagccg ggacattatt gccgccgcat gcagagcctt aaaccatgtc tggcgtgaag
    15301 ggtaccgcta tatgaaggca ggagtcatgc tggctgattt cacaccatcg ggtatagcgc
    15361 agccgggatt atttgatgaa atccagcccc gtaaaaacag tgaaaagtta atgaaaacac
    15421 tcgatgaact gaaccagtcg ggaaaaggga aagtgtggtt tgcggggcga ggaaccgccc
    15481 tgaatggcaa atgaaacggg aaatgttgag tcagtgttat acaactaaat ggcgagatat
    15541 tcccctggcc aggctgggtt agttcagtca tctccgaaca tattttcagc gtttcttctg
    15601 gtctggtctt caccgtcatt tctcgacaga ctctgctctg taaggggagt actgcaatcc
    15661 acagacgtat ggagaccaga ttgtttttcc agtaattcaa tcaatttctt ttcaaccgaa
    15721 aaagcaggta atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac
    15781 tttgtcatgc agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag
    15841 gtgctgcctc agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac
    15901 gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac
    15961 cacgtcaaag ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc
    16021 gaatacgtgc gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg
    16081 gcgcgattta gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtg
    16141 ctgtcacacg agtatggagt cgttctttaa caagcaatac gtaac
//
