LOCUS       CP141467               20691 bp    DNA     circular BCT 10-JAN-2024
DEFINITION  Staphylococcus aureus strain Sau49 plasmid unnamed1, complete
            sequence.
ACCESSION   CP141467
VERSION     CP141467.1
DBLINK      BioProject: PRJNA1046723
            BioSample: SAMN38508018
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Nyasinga,J., Munshi,Z., Kigen,C., Nyerere,A., Musila,L. and
            Revathi,G.
  TITLE     Displacement of hospital-acquired methicillin-resistant
            Staphylococcus aureus clones by heterogenous community strains in
            Kenya over a 13-year period
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20691)
  AUTHORS   Nyasinga,J., Munshi,Z., Kigen,C., Musila,L. and Rivathi,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2023) Pathology, Aga Khan University, Nairobi, 3rd
            Parklands Avenue, Nairobi, Nairobi 00100, Kenya
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-MAR-2023
            Assembly Method                   :: FLYE v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 23x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/06/2023 16:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 1,754
            CDSs (with protein)               :: 1,754
            Genes (RNA)                       :: 83
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1,029
            CDSs (without protein)            :: 1,029
            Pseudo Genes (ambiguous residues) :: 0 of 1,029
            Pseudo Genes (frameshifted)       :: 978 of 1,029
            Pseudo Genes (incomplete)         :: 54 of 1,029
            Pseudo Genes (internal stop)      :: 33 of 1,029
            Pseudo Genes (multiple problems)  :: 31 of 1,029
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            NCBI analysis of this genome has determined that >30% of the
            predicted CDS features contain frameshifts, which is well above the
            usually observed value of <10%.
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Sau49"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Kenya: Kajiado"
                     /lat_lon="1.8421 S 36.7919 E"
                     /collection_date="2022"
                     /collected_by="Aga Khan University Hospital, Nairobi"
                     /note="genotype: MRSA"
     gene            join(20573..20691,1..717)
                     /gene="blaZ"
                     /locus_tag="UM789_14200"
                     /pseudo=""
     CDS             join(20573..20691,1..717)
                     /gene="blaZ"
                     /locus_tag="UM789_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /name="blaZ"
     gene            1086..1694
                     /locus_tag="UM789_14205"
     CDS             1086..1694
                     /locus_tag="UM789_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WRN52189.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            2106..2399
                     /locus_tag="UM789_14210"
     CDS             2106..2399
                     /locus_tag="UM789_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WRN52182.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            2474..2776
                     /locus_tag="UM789_14215"
     CDS             2474..2776
                     /locus_tag="UM789_14215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WRN52183.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKNFLFSTQNEIILTLYVIKHHF"
                     /name="hypothetical protein"
     gene            3674..4405
                     /locus_tag="UM789_14220"
     CDS             3674..4405
                     /locus_tag="UM789_14220"
                     /inference="COORDINATES: protein motif:HMM:NF018897.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WRN52184.1"
                     /translation="MIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQ
                     VLSKNVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQ
                     LLKDNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFS
                     WNKKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQH
                     LLQIAYIKYLEKYFKDLMREI"
                     /name="DUF1430 domain-containing protein"
     gene            4409..4624
                     /locus_tag="UM789_14225"
     CDS             4409..4624
                     /locus_tag="UM789_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WRN52185.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKRVINWIFRL"
                     /name="YxeA family protein"
     gene            4730..5355
                     /locus_tag="UM789_14230"
                     /pseudo=""
     CDS             4730..5355
                     /locus_tag="UM789_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /name="ABC transporter ATP-binding protein"
     gene            5592..6520
                     /gene="mobV"
                     /locus_tag="UM789_14235"
                     /pseudo=""
     CDS             5592..6520
                     /gene="mobV"
                     /locus_tag="UM789_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_process: GO:0006310 - DNA recombination
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            6521..6859
                     /locus_tag="UM789_14240"
     CDS             6521..6859
                     /locus_tag="UM789_14240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="WRN52191.1"
                     /translation="MTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKELNYTLKLFNQ
                     VFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQAQEREIIFKE
                     DREDGYTL"
                     /name="recombinase"
     gene            7242..8102
                     /locus_tag="UM789_14245"
     CDS             7242..8102
                     /locus_tag="UM789_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WRN52186.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            8189..8530
                     /locus_tag="UM789_14250"
     CDS             8189..8530
                     /locus_tag="UM789_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WRN52187.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(8769..8840)
                     /locus_tag="UM789_14255"
     CDS             complement(8769..8840)
                     /locus_tag="UM789_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WRN52190.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            8989..9174
                     /locus_tag="UM789_14260"
     CDS             8989..9174
                     /locus_tag="UM789_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WRN52188.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(9393..10135)
                     /locus_tag="UM789_14265"
                     /pseudo=""
     CDS             complement(9393..10135)
                     /locus_tag="UM789_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            10545..11221
                     /locus_tag="UM789_14270"
                     /pseudo=""
     CDS             10545..11221
                     /locus_tag="UM789_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            11232..11514
                     /locus_tag="UM789_14275"
                     /pseudo=""
     CDS             11232..11514
                     /locus_tag="UM789_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_process: GO:0006260 - DNA replication
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            12719..13335
                     /gene="cadD"
                     /locus_tag="UM789_14280"
                     /pseudo=""
     CDS             12719..13335
                     /gene="cadD"
                     /locus_tag="UM789_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /name="cadD"
     gene            13354..13701
                     /locus_tag="UM789_14285"
     CDS             13354..13701
                     /locus_tag="UM789_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO_process: GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WRN52174.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            13870..13962
                     /locus_tag="UM789_14290"
     CDS             13870..13962
                     /locus_tag="UM789_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WRN52175.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            14174..14833
                     /locus_tag="UM789_14295"
     CDS             14174..14833
                     /locus_tag="UM789_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WRN52176.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFISSTHHLMIVILKI"
                     /name="DUF4062 domain-containing protein"
     gene            14878..15042
                     /locus_tag="UM789_14300"
     CDS             14878..15042
                     /locus_tag="UM789_14300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WRN52177.1"
                     /translation="MKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLK
                     LLQKIIRLR"
                     /name="hypothetical protein"
     gene            complement(15266..16081)
                     /locus_tag="UM789_14305"
     CDS             complement(15266..16081)
                     /locus_tag="UM789_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WRN52178.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(16074..17515)
                     /locus_tag="UM789_14310"
                     /pseudo=""
     CDS             complement(16074..17515)
                     /locus_tag="UM789_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(17487..18080)
                     /locus_tag="UM789_14315"
     CDS             complement(17487..18080)
                     /locus_tag="UM789_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WRN52179.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(18343..18723)
                     /gene="blaI"
                     /locus_tag="UM789_14320"
     ARG             complement(18347..18723)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(18713..19507)
                     /locus_tag="UM789_14325"
     CDS             complement(18713..19507)
                     /locus_tag="UM789_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013100.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008658 - penicillin binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="WRN52181.1"
                     /translation="MIIQSQFLMGQSLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMY
                     SMKKDKYYIYNEKESRKRYSPDSTYKIYLALFGLDRHIISDKNSRMSWNHKHYLFESWN
                     KEQDLNTAMQNSVNWYFERISNQIPKNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISN
                     LEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVG
                     YVITNHDKYYFATHLSDGNPSGKNAELISEKILKGMGVLNGQ"
                     /name="penicillin-binding transpeptidase domain-containing
                     protein"
     gene            complement(19504..20508)
                     /locus_tag="UM789_14330"
                     /pseudo=""
     CDS             complement(19504..20508)
                     /locus_tag="UM789_14330"
                     /inference="COORDINATES: protein motif:HMM:NF017390.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /name="M56 family metallopeptidase"
     ARG             476..717
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="97.5"
                     /coverage="28.47"
     ARG             4..302
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="95.83"
                     /coverage="35.23"
     BGC             1..7399
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          11570..11716
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="1.06e-24"
     Typing          7243..8102
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9394..10135)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.731"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5593..5867
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 tagatactaa aagtggtaag gaagtaaaat ttaattcaga taagagattt gcctatgctt
       61 cgacttcaaa agcgataaat agtgctattt tgttagaaca agtaccttat aataagttaa
      121 ataaaaaagt acatattaac aaagatgata tagttgctta ttctcctatt ttagaaaaat
      181 atgtaggaaa agacatcact ttaaaagaac ttattgaggc ttcaatgaca tatagtgata
      241 atacagcaaa caataaaatt ataaaagaaa tcggtggaat caaaaagtta aacaacgtct
      301 aaaagaacta ggagataaag taacaaatcc agttagatat gagatagaat taaattacta
      361 ttcgccaaag agcaaaaaag atacttcaac gcctgctgct ttcggcaaga ctttaaataa
      421 acttatcgca aatggaaaat taagcaaaaa aataaaaatt tcttacttga tttaatgttt
      481 aataataaaa acggagacac tttaattaaa gatggtgttc caaaagacta taaggttgct
      541 gataaaagtg gtcaagcaat aacatatgct tctagaaatg atgttgcttt tgtttatcct
      601 aagggccaat ctgaacctat tgttttagtc atttttacga ataaagacaa taaaagtgat
      661 aagccaaatg ataagttgat aagtgaaacc gccaagagtg taatgaagga attttaatat
      721 tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt tctattatcg
      781 ttgacatgta taattttgat atcaaaacct gattttccct ttattatttt cgagatttat
      841 tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag
      901 atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg
      961 cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca aagtgacata
     1021 attgttgaat ttatagtaac tgaaacgtat gattagggtg tatattaatt atgagatgga
     1081 aatcaatgat tgttggctat gcgagagtat catcgaacga tcaaaattta gaaagacaac
     1141 ttgaaaattt aaagacgttt tgtgctgaaa aaatatttac agaaaaacag tctgggaaat
     1201 ctattgaaaa tagaccagta cttcaagaag cacttaactt tttgaggatg ggagatcggt
     1261 ttgtagtaga atccattgat tgtttaggtc gtaattatga tgaagtgatt cataccgtta
     1321 attatttaaa agacaaagaa gtacaattaa tgattactag cttacctatg atgaatgaag
     1381 tggttggcaa tccattactg gataagttta tgaaagattt aattattcaa atattagcaa
     1441 tggtttcaga acaagagaga aatgaaagta aacgtcgaca agcgcaagga attcaagttg
     1501 cgaaggaaaa aggcgtatat aaagggcgcc ccttacttta ttcaccaaat gctaaagatc
     1561 cacagaaacg tattatttat catcgcgttg tagaaatgtt agaagaaggc caatcaatta
     1621 gtaaaattgc gaaagaagta aatataacaa ggcagacgat ttacagaatt aaaaacgata
     1681 atgatttgat ttaataaact ttgtttcaat gcaactgaga gcttctcaca gcgtctcaaa
     1741 acagatgatt ataatctatt atgagttttt aataataaga caagaaaaga gagcgtaaat
     1801 atgctctctt tttctcatta acgaatgttt ataacgcaat catgatgaga gtattaatac
     1861 agttaattat ttgaaatact ttagtttata taatgttgaa ttataggtag tgaaattgta
     1921 tttattatac attgtcatat cgttgtttaa tgatagttaa tttaaggata atagaattaa
     1981 tattatatat agatgaacaa aacaagcaac agagcagaaa aaacactttt agattatttt
     2041 tactcattct ctattttatt tatttttgct ataataaata aagaatatta aatgtaggtg
     2101 tttttatgaa aaagaaattt gtttctagtt gtattgctag tacaatctta ttcggcactt
     2161 tattaggtgt aacttataaa gctgaagctg caacagtaca tgtagctggt ggtgtttgga
     2221 gccatggtat tggaaaacat tacgtatggt cttattatag tcacaataaa agaaatcatg
     2281 gttcaacagc agtagggaaa tattcatctt ttagtggtgt tgctcgacct ggtgttcaat
     2341 ctaaggcatc tgctccaaag gcttggggtg gaaacaaaac attttatagt cttcactaat
     2401 tacatatatt aattaaggag aatagacaga attaagtaaa cttaattctg tctattctct
     2461 tgaaagaggc aatatgaaaa aattcaaatt atttttagat tttagtactt tattattaat
     2521 ttcagggcta ttatttttat ttttcttcaa agaaaatgaa gaaattatac ctgaaagttc
     2581 caatatttta acaatctcta attgggataa atctaacagt aaatcaaaag tgttagatgt
     2641 cattgaatca ggcgccaaaa atcaaaatat acaaataatt aaatccgtta aagattttga
     2701 taataaaaag aattttttgt tttcaactca aaacgaaata attctgactt tatacgtaat
     2761 aaaacatcac ttttaactcc atcagattta ttaaacagag agataaaagg aaagtattat
     2821 attattggtg aacatttcaa tgtagaagag cttacgagtg aattaaaaaa gctggtttaa
     2881 catctgaagt tgaatacata aatagaaaca tattattttt tgaattagtc atagataatg
     2941 atttattagt gcctttcata ttcttaggcg tattatatct tttgtacttc ttatatgaca
     3001 gaggatataa tttgaaattt tatgctattc aaaagctaca tggcttttca actacacgca
     3061 ttatttttca taatattcat ataaaaatca tttattggct agtacttacc gctattttct
     3121 ttattgctaa tatattaatc attcatatag ccaatttaga attggatttc ttaatgttta
     3181 ttcgtagatt tatcgtgttg ctttttgttt ttgcttgtat tactacattg ctgtggctga
     3241 tttcgtatag tcttcttttg aagttaagta ttccattaat cttaaaaggg aaaaaaggat
     3301 ataagttttc aatatttatg ggtaccttca ccaaatgtat catggtgcta atactatctt
     3361 ttttactggc acaaaattta aatgcttata cgaagttaaa gaatatatat gactctgaaa
     3421 aatctggcaa aagttagata attactatac attagagatt tctcctaata ttagaaattc
     3481 agaagaaaaa caaattttag aaactaatat atacaattta attaagaaaa gcgaacaact
     3541 tggtggattg cttttaaaga ataataatat agccaatccc gataaaaaca attacattcc
     3601 tgaaaatggc aatgtctttt ttatcaataa caattttctg aatttctata aaaatataaa
     3661 tcatgatttt aaaatgattg acaatcattc ggataaaata aacgtgttta ttcctccaag
     3721 gttacaatat caaaaatctg aaattcaaaa aaatcaccaa ggatggattg actttcaaaa
     3781 gcaacaaaac aaaaaagtag atgtgcaagt actgtcaaaa aacgtatctg ttttttcttt
     3841 tgatcgcaca actaatttga aatttgggta tctagattct ccaatagtaa tgttgttgac
     3901 acttgacgat ttaacaggag atttttattt agctgctgtt tctcaaggtg gatatctatt
     3961 taaagattta gatacactta aacagttgct taaagataat cacctagaag aagaaattag
     4021 cggtataact aattacaagg attctgtgtt aaatgaatta aatgaaacga aaacaaaaat
     4081 gataataaca atagttacta ttattattaa cgttttcatt cttttgatag ctactgtatt
     4141 tgaaacaatt caatatttta gctggaataa aaagcaacta ttattaagaa aaattcatgg
     4201 ttattcatta ttcagcagta atgtacgtta tttaacaatt tctatcttac tttctattat
     4261 gttagcttac actacgcata ttttatttgg ttcaaagata ttactattca taataatgtc
     4321 tattgctatt attcaacatt tacttcaaat tgcttatatt aaatatcttg aaaaatactt
     4381 taaagattta atgagggaga tttaaacaat gaaaagaact ttaattattg taacaattgt
     4441 actattaatc atcatactta gtctgttatt ggttagaaat gagcatttag atcgttttaa
     4501 cccactgtta aaagaaaaag tatcttatgc caaagttcca aaagataccc aggactatag
     4561 aaatataact gtatactcta aaaatggaga aaaaagagtt ataaattgga tttttaggtt
     4621 atgatcctac aaaagaatat gtgaaagtaa atcataaagg gaaatatgtg cgttcgatag
     4681 aatacattac aaaagatatt cctagctttt taaaatgagg tgaccgatag tgataaaact
     4741 tgaaaatgtt tttgtaaaaa aggtaataaa aacattttag acggatgtaa ctttaatttt
     4801 gaaaaaggaa aatcatatgc tttagttgga gaaagtggcg ctggaaaatc tactttactt
     4861 aacataatcg ctgggttcga agatgtatca caaggaagca tatatattga agataagtta
     4921 ctaaaaaaga aagtcgattt ttatagatat acgttagggt atttgtttca aaattttgct
     4981 ttacttgaaa atcaaacaat cagtcaaaat ttagatttag ccctaaagtt caaaaagaat
     5041 aaaaaagata atatgaactt aaagaaagaa gtattaaaaa aggtcggcct agatttagat
     5101 atcaagagaa ttgttagttc tctaagcggt ggtgaacaac aaagagtagc attggcaaga
     5161 ttaatattaa aagatccgaa aatcatttta gctgatgaac ctactgggtc actagatacg
     5221 aaaaatggaa aaatagttat tgatttatta ttgaagctac ttgatgaaaa taaaactatg
     5281 attgtcgtaa cgcatgattt agagcttgcg aaacgatttg atgtcatagt caatatttct
     5341 gagcttagaa gttaagaaat attgataggt atagccatac atagagaaaa acaatatgaa
     5401 atatgattag tcattacttt tgagtttttt aatttaagga ctggattttc cagtcctttt
     5461 ttatgatgac aaaaaacaag cctcgcagag cacacgtttt taatgactta tttggttttt
     5521 aataagtcta gtatgttaaa acttttctgt gtttaggtgt tataatcata tgcagtggaa
     5581 gttgatttat tatgtcttat tcaattattc gagttgttaa agtgaaatca aaaacaaata
     5641 caagaggtat tcaaagacat attcaaagag aaaataaaaa ttatgaaaat atagatattg
     5701 atttaagtaa gtcatattta aactatgatt tagtcaatga tactaagttt gattttaata
     5761 aaaaaattga tgagaaaatc gaaaaaaatt ataaagggaa acgcaaaatt agaacagatg
     5821 ccattaaaca tattgatgga ttaattacat cagataatgt gttttttaat caattatctg
     5881 aagaagagac aaaatagttt tttaagcatg ctaaagcgtt cttggaagaa gaatacggta
     5941 aagaaaatct tttatatgcg acagtacata tggatgagat aacaccacat atgcattatg
     6001 gcgtagttcc tattactaaa gatggacgtt taagtgcgaa agaagttgta ggtaataaaa
     6061 aagcattaac cgaattccaa gatagattca atacgtatat taataagcaa ggctatgatt
     6121 taaaacgagg aatttctaga caacttacaa aagaaaaaca tgatcaagtt agccgttata
     6181 aacaaaaaac agaatatcat aaacaaatgc atatgcgcta gaagcaaata gaggattatt
     6241 taaagcaaga aaatgataaa ttgatacaaa actaccaaaa atcattagat acgcttaaaa
     6301 agcttctgag tgtcatgtat gaaattaaaa ctaaaaatgt aggtggatgg tttcataaag
     6361 agaagcaaga aacaggcaat attgtaatta ctaaaactga ttttgaaaaa tacactaaac
     6421 aaattaaagc agcacagatg atattagatg attatgaatg tattaaaagt gggaagtcat
     6481 taaaaaagca gagaaacaaa atgaaagttt agttaatgaa ttgactagtg tccatatgga
     6541 aaatgaaaag ctggttgaag aatttaatga tttagcacaa cgttataatt atttgttgtc
     6601 agagaatgaa aagaaagata aagaactaaa ttatacgctt aagttattca atcaagtttt
     6661 taaaatcatt aaaagtatga tgaaagaaga gcgttatcat acactaataa atcatattga
     6721 taatcattta gacaattcta aaatacgtga agtaatgacg atagataaca atgatgaaca
     6781 gttctttaag aagaaatatc aagcacaaga acgagaaatc atttttaaag aagatagaga
     6841 agatggttat acattataaa aatagatatt taaatgcatt tgtttgaggt taattatttt
     6901 ttaggatgaa aaataggtag ccccctcgaa ctttttaata tatttttata aatcttttta
     6961 aaaacctttt taggggcttg ttaatcccta gagcaacaag ggatttcaat gcctaaagga
     7021 gtgtttttgc cacataaaag gagtgttttt gccacataaa aggagtgttt ttgccacata
     7081 aaaggagtgt ttttgccaca taaaaggagt gttttaaaaa gttgtactcc ttttatggtt
     7141 tgacattgat aataaagtag tttattgtga ataaaagttt tcaaatggga gtgtatattc
     7201 aaacgatact cctttaggtt cgtaataaag ggggctgtat catgacagga gagacagttg
     7261 tatataaaaa tgaaatgaat ttagttccat taaggcgatt tacagctact gaaattaatt
     7321 tattttttgc gatgtgtaat aaattaaaag agcaagatac taatacatta cgtttgtctt
     7381 ttgatgaatt aaaaaaatta agtaattata gtccagaaac acgtaatatt aatagatttg
     7441 ctaatgattt agataatgtg tataaaaaga tgttgaattt aaccattaga tatgaagatg
     7501 atgatgtaat agaaagattc gttttattca atcattatag aatccataag cgagaacaat
     7561 atctagaaat atcaacttct tcaaatttaa aacacatatt gaattcaatt actaataatt
     7621 ttacaaaatt tgaattaaaa gaaatgacta ggcttaaatc tacatattct aaaaatatgt
     7681 ttagattact taaacaatac aaacatacag gctatttaaa aattcacatc gatgatttta
     7741 aaaatcgttt agatatacct aaatcttatc gtatgactga tattaacaaa aatgttttta
     7801 aacctattat tattgaatta ggttctatat ttaataatct tactatcaat aaaatcaaag
     7861 cgaaaaaagg gcgtaaaatt gaatggatag agttcacatt tgacgctgag aaacgcattc
     7921 ataacaaacg acaacctcaa atgtccaaga tagataaatc aagacaatat gtgaggcgtg
     7981 aaaagacgcc taaatggtta gaagaacggt catatgaaaa gcaacctcaa aaagattacg
     8041 acccacaatt agagaaagaa cgagaggatt ttttaaaaca acttgaactg aattgggaat
     8101 aacgcaactt ttcgttgcga gttggttaac taaaagtatc gtgacttttc aattatttta
     8161 attatagttt gagttaagga gtgattttat gaaattagct gaagctataa aagaacagag
     8221 agaattaaaa ggatggtctc aagaggaatt agccaatata ttaaaggttt ccagacaaag
     8281 tgtttctaaa tgggaaagtg ccaaaaacta tccttcctta gatattttga ttgctatgag
     8341 cgatttattc ggaatctctt tagagcattt aattaaagga gatgcacgtt ttaaaaaagt
     8401 tattttagag ggaaattata gagagagtaa cagggtacct tccatagttg attttcttta
     8461 tgatttttgg tggcttttct ttcctgttgc cggattttta gtttgggcca ttcattcgtt
     8521 catgggttga ttttaaaatc aaaatttaat gcacatcagg caatatgaaa aaggaaagta
     8581 tttttacgac accttgaaat gtattgataa gaatagaaga gtttacaaaa ggcaatatga
     8641 aatatatact aaaagtaaca agaatttcgt ttgtaaacgc acaaaacccc gaactattcg
     8701 ccgtagttcg gggttttttg gtgaattggc tttaatcgtc tatattattt tttatcacgt
     8761 ttatggagct aataaccaaa taacgtaacg agacaaccac tgattgccgt tgtcatgatg
     8821 tgaacaagaa tttcattcat aaatcgcacc tcctctctac gccgaattga catctgagag
     8881 atagacgacc ttcacattat aacaaatctc tccttccatg aaataacatg aatattaata
     8941 aatgttgtta tatattaaag ataaataaat ataagggtgg gattgataat gtttggcgat
     9001 caaagacaag aagctacaaa atatgtcatt aaagaaggtt atcaagatat ttatttcctc
     9061 aacaaaaacg gggaatggta ttatttcgaa gtaagaagtg tctggcgtgg caaacatatt
     9121 attagagtta aagatggttt gttaggttgg agaaaagaaa tagtaactga ataaagatac
     9181 aaaatacttg ccaatgcaaa agaatgaaaa cgcgtcaaat caacgatgta taaaagatgc
     9241 ataaaagata atgaaagtaa aaagccataa atgccgtcat ataggcattt atggcttttt
     9301 gtttggttaa cataatcgat gattatcaga agttcaattc acataaaaaa gcacggctaa
     9361 tttatgaact tgtcgtacct ttttaattaa aattagttac caaatagacg actccaaaac
     9421 ccctttttag gttccgtttc ttcactcttt tcttctatat cactatcatt gtcatttgga
     9481 ttagctgaaa cgtcttctct ttgtgcttct tcaccttgtg tactttcgtc gctttgactt
     9541 ccagatatat ccttatattg aatctctgtt tctttagttt cttcctgatc tcggttaata
     9601 tttacaggac tagtagtata tgttttttct tgtgcatcaa cgttttgtct agcgttagta
     9661 gatgtatcaa agggataatt aaggtgtttt tctttttcta attgattttc taatttttga
     9721 atctttttat tactctctaa tgccagaact tgctgattct caagcaattt tgatctttct
     9781 tgcaactctt ttctgtaaaa ttcaattgtt gtttcatgac gactttcttg tttttcaatt
     9841 tgattattta aagtattaat ctgattttta agtatttcta ctatttgagc atcgctttta
     9901 gattcaaaat taatattatc cgactctttt tttgtttgtc ttcagtagca ctttcaaaac
     9961 catatttctt tttattttca tttactcttt gaatgatttt ttctatatcg gtatcttctt
    10021 taataaacga agtgttttcc tgttttattg tctctatatt caatctctta atattattaa
    10081 aaatcgtttg cttactaacc cctaattctt cactcaacct ttttacactt ttcatatcat
    10141 caacccttta taaaatgttt ataaaccctt agtaaaatat tataagctta taacgattaa
    10201 taataaagta atcgacaaat aaaaatataa attcgccaac ctttttatca agggtttgta
    10261 aagggtttat ggtgtttatc aaaggtttat aaacctatat tttccctttt aaagtttatt
    10321 caaagtgcta taataacctt aatataacaa aaaccccaac tactggtaat agtcggggtt
    10381 tggtaataaa acgtttagaa actgtttatt tatataattt tcgatactta gattatataa
    10441 tgaataatat cttagtgcaa gcacctagat tattaaataa acacaaaaaa tgtaagcgtt
    10501 ttattacctc ttaccaaact aaaattcgga aagaggtttt tattatgtca attaaacttt
    10561 acaacataaa tgaggaatac agagaaaagt tttatcaaat accgaaagta ttttttacaa
    10621 atcctaaata tatcaagtta tccaatgatg caaaaatggc atggggtatt cttagagatc
    10681 gtttagattt atcaattaaa aatggttggg ttgattctaa aacaggtaat atttattttt
    10741 attacaaaaa tgaaaaccca caaaatatat taaattgtaa aaaagataaa gtaattaaaa
    10801 taaagaaaga acttaattga agccgaatta ctactacaag aaagacaagg tttaaataag
    10861 acaaataaac tttatttagt taaaccagaa gtaacagaag atgatattta taaaattcaa
    10921 aagcaagaaa acactgttga gagcagcaat ggcaaggaag tcggaaaaac cgacttcaag
    10981 aagtcgataa aacagaatct aatgatactg aacttagcta tactgaatct aacaatactg
    11041 aaataaatga tatgaatgac ttgaatgata tagagtccga aaatgaaatt agtaatcatt
    11101 caagtcaatg caaggttatt taatgcaaac aattttaact gaatttgaag aaacacatgc
    11161 actttatatg cgccgtaaaa acatgaaaca atataatatc tttaatcaat cattcaaatg
    11221 attttataac aatcaaagat acatcaaaat atttcgttaa atcatctgac tttttaactg
    11281 atagcgatga aaaaatcaag aaattgtcag tgatctagaa aaagatttgt atcgaaaacg
    11341 tatgttaagt tatggtggat tgcttaaata aaaacataaa attttaaact tagacgatgc
    11401 tgaagatggc aatttaatta atacaagtga tgaagataaa atagcaaatg aagaagaaaa
    11461 ggcacattcg attacggcaa ttttgaaaag caaaattatt atttaaggca ttagtgataa
    11521 ctgatgtctt ttttattact tttttgtaaa aaagtgctgt cttatttttg tgacaaatgc
    11581 tgtatgtagt gtcacaaaag tgtgacactt gagcttttat gaccccacac aaaaacattt
    11641 tcgaagcctt gaaatatctg gctttgccag atcccccatt cattgatggg gacaaatttc
    11701 ccttatgctc ttacggagat tttagagtta aattaaaatt tctaagtttt tgataagaaa
    11761 agccctgcag gaatttagaa gaacgtgggg aagttttaaa gaattttatg ctaattttta
    11821 atttgcatgt aaatgggcag tgtctaaaaa atagttattg tttttgctca aatttttgtt
    11881 tttgaaaata gcatatattt atttggctca tatttgcgtt ttaaaagcgt atataagttt
    11941 tagatataaa actatatgaa tcacccctaa atctttaaaa tgccctctta aaattcaaaa
    12001 taagggcata aaaaataaaa gccccccaga taaagtgagg tagctttttc tttcgacgcc
    12061 gtatcgtcga tgtaattcaa aaagttatgg gctataaatc tacatcactt tttcaagaat
    12121 gtagtagcaa tattcaacgc aaattaattt ttataactgc tcggaatatc tcaagccgtt
    12181 tctttaattt tgaaataaaa aatcgacgaa ggtcgattac gtttttgcac atgtcgattt
    12241 atccgacgta taagtgcgcc cttacgggat ttaactagat tataacgacg aattttagac
    12301 ctgtaaagca ataacaatta ggtctaccag gtagataaag tgtacaaagt agaaccgaaa
    12361 aagttattta ttttgaagat ttttcgtaga tataatttgc taataaactt cctaaaactg
    12421 aataaagaaa aatagttaaa ctcatgtaat cactccataa ttctgaagtg tctccatgtc
    12481 tacgcataga cacgcattta tgccgagaaa atttattgtg cgttgagaag aacccttaac
    12541 taaacttgca gacgaatgtc ggcatagcgt gagctattaa gccgaccatt cgacaagttt
    12601 tgggattgtt aagggttccg aggctcaacg tcaataaagc aattggaata aagcacctat
    12661 gattatttaa aaatataagt tataatacat tcaaaaatat ttttgaatga ggtgtattat
    12721 gattcaaacg gttgttgctg ctgctgttct ttatattgct acagcagttg atttattagt
    12781 aattttatta atattttttg ctagagcaaa gactagaaaa gaatatagaa atatttatgt
    12841 tggtcaatat ttagggtcta ttattttaat attagttagt ttgtttttag cttttgtatt
    12901 aaattatgtt ccagaaaagt ggatattagg tttattgggt ttaataccaa tttatcttgg
    12961 aattaaagtg gctatttatg atgattgtga aggagaaaag agagctaaaa agaattgaat
    13021 gaaaaaggat tgtctaaatt agttggtacg gttgcaattg ttacgatagc aagttgtggc
    13081 gccgataata ttggtttatt tgttccgtat tttgtgacat taagtgttac taatttatta
    13141 cttactctgt ttgtcttttt aattttaatt ttcttcttgg tgtttactgc acaaaaatta
    13201 gctaatatcc caggaattgg agaaattgtt gagaaattta gtcgttggat tatggctatc
    13261 atttatatag ctttaggttt atttattatt attgaaaatg acactattca aacaatttta
    13321 ggatttatat tttaatttag ggtgtgattt tatatgagtt atgaaaatac ttgtgatgtg
    13381 atctgtgtac atgaggataa agttaacaat gctttaagtt ttttagaaga tgataaatct
    13441 aagaaattac ttaacatttt agaaaaaatt tgtgatgaga agaaattgaa aatcatatta
    13501 tctttgatta aagaagatga gttgtgtgtt tgtgatattt ctttgatatt gaaaatgagt
    13561 gttgcttcaa cttcacatca tttaaggctt ttatataaaa atgaggtact tgatttttat
    13621 aaagatggaa agatggcata ttattttatt aaagacgatg aaataagaga gtttttctct
    13681 aaaaatcagg agggtttttg aaacgagtga aacgagtttc ttcttgtctt gatactataa
    13741 aaaacaaagt tatataacat aagacgactt atcggaagtt atctatcatc gtatattcgg
    13801 agatgatata atatacaggt cgcctatctc tcaggcgtca attcagacgc agagaggagg
    13861 tgcattactt tgctaatcat tttcgttcac atcatagcac cagtcatcag tggctgtgct
    13921 gttgcgtatt atacttattg gcttagtaaa cgcaacaaat aattaatagg cgactttata
    13981 agccagcatc gaaaaaatcc ccacactatt gcagtagtgt ggggattggt gcattaattg
    14041 ttaatcattt tcgttatcta gattataaca ctggttagta gaggaatgca aaaagataat
    14101 attttaagta gtctttttgg atattatcat atgatatatt tatgtaaatt gattttttat
    14161 taaaggagca tatatgagta atactaaaat taaatatcaa atttttattt catcaactta
    14221 tttagattta atagaagagc gtcaagcagt agtagaagca gttttaaaca gtaataatat
    14281 tccagcaggt atggaacttt ttaactcaag tagtaaaagc caatgggaaa taataacaca
    14341 atggatcaat gaatcagatg cagtgatatt attgttaggt tctagttatg gaacaattga
    14401 tagtgaagaa aatattagct atacagaaat ggagtacaat tttgctaaat ctttaggtaa
    14461 acctatatat gtttttgaga tgaataaagc atctaccgaa tccgcggaaa agtctaaaaa
    14521 attagatgag tttaagacaa aagttaaaga aaatagacta attagtccaa atattaatga
    14581 tatttcggaa ttaaaaagta aagtatctca gactttaatg caaagtgtac ctagtgaaat
    14641 taaagaaaat agtggatgga taagagtaca ggattgtggt aaaaatgtaa aaaaattaaa
    14701 aaaattggat gctatacaaa gagatttaga tacatatgat tttttgaaag aagaacttta
    14761 taaaatattc atgtatgtaa aaacttttat ttccagcaca caccatttga tgatagttat
    14821 attgaagata tagaaaacta cctgctaaat tatgaaaata atccacataa atcattcatg
    14881 aaaataaaag tttatgatta taaaaacttt ttaaaacatt taaaaaacat attgatttta
    14941 attaaacaag gaaaaaagcc attagctcaa gccggtcatt ttaaattaag cttttacgaa
    15001 gaggatttaa aattgttaca aaaaattata agactacgct agtcgatatt aaaggattga
    15061 ataaacattt ttataaggaa tgtgataggt gcttaaaagc atataatatt tttattgagg
    15121 gtataaataa acatttatat gaatgatata agtacaccct aatcatacat atataattgt
    15181 tgtcacttta ctcgatatat gagaatgact ttacctaata aatgagaaaa gcaacgcact
    15241 atattaaaat gcgttgcttt ttctattatc gaatacctat aactaaacta tctctagctg
    15301 tttctacaac ttctgtactt attttatcta aaccatttat atccataatt ctgtctatct
    15361 gcgcaaataa acgatgaatc agcctaaaat tcccttttgt aattttaata atcgtcgtta
    15421 ttgcctcata atcagtaaag tcttcgagtt ttaaatcaaa tcctaagtct tgccacttat
    15481 attctaatat atgatgcgtc tcatctttac ttagattatc aaattcatgc gcaaaaccta
    15541 tacgcgaata tagttgagga taacgagata gttttttctc tatacccggc ataccaataa
    15601 atatcattgc taaattatgt tcatcataaa tagctcttaa ttgctctaaa tgttgcactt
    15661 taaggcgatc aatctcatct actataatta aatcaatata tttaaacata tcactataat
    15721 gtttactatg ttcatgtcca tacttattta caacatgtaa tttttgaccc aaatcaatga
    15781 ttgcagtaat actatataaa tcattcctta atttagtttg tttttctgct ggtgcattat
    15841 aaaatatttt atccaccgat agtatgtcat cggtcgtttt actagctaaa tcttcccaac
    15901 ctctatggtt aacttgtttt tcaatagtat cccaatttgt gtaataccta gacgataaag
    15961 tttttccgac accaggttga ccataacata taccaatata ctgatattta atacaagcat
    16021 cacaaaattc agcaaagcgt ttatactctt tcgtttcaat gaaattttga tttttagtca
    16081 ttacgatatc tctttaattt tttgacgttt ttagtcgttt cttgaggtga ataaccacaa
    16141 ttttttcttc cttaattaat tccgtggtac ttggactagc aatattttgt tttaagtgtt
    16201 ttcttctctg actacgagcg tgctgtatgt cttttatatc tatggagtaa tcagctattt
    16261 ctggacatat agcagtacaa agaaattcat ctctataaaa cacacgtatt tcagccatat
    16321 catttgggtt ataacgaatc agtacatatt cacctacata agcttctaga ttagtattac
    16381 tgtatcgaaa accttgaaaa tgaatgccat ctgaatgaac ttttctagat ttaggtattt
    16441 ccaacaataa taaatcaagt tgttctaagc tacttggcat attagggaaa aagtgattac
    16501 tattccaatg actgataggc gtggtatgaa tagtactatg ttctttttga ttataatctt
    16561 caattaaaaa gtaacgtaat ttttcttcaa aattgtgaaa atttattaga ttactaggag
    16621 tatcattatt gtttatatat ccaggcagtt gttctaagaa agtttgatta actgtctgaa
    16681 agaatcgttc tatttttcca cgaccacgag gaacaccaac tttcgaaaac atcaagttaa
    16741 ttttcaaatc aatagcgact tgttccatat gatgtgaagt aaagtcactt ccatgatctg
    16801 tgtagaattt ttcgggtata ccacacactg gccaattggt atcattttta ttccatattg
    16861 cttggtgtag tgttaatgct gtgttttgag cattgggggc atcaaaactt aagaaatagc
    16921 ctgcaattgc acgactatag tcatccataa taatagttag ccatggtcta ttgatattac
    16981 ctttttggtc taatataaag atatctagca aagtatggtc tgcttgccat atttcattgg
    17041 gtctcgagga ttctctaatt tgtattaaat cgtacttatt ttgatagtat ttttcaccct
    17101 gatgagagaa atcaataaca gacttaggca tcgctttaat aacactataa atttgtttat
    17161 aacttggtat atcaaaatta ttttctttac agtaattagt gattttccta tgaattgtgg
    17221 ctatactatt tcttttattc ataagcgcaa ggcgttcaat ttcatcaatt atctttggtt
    17281 ctattttacg tgttccttta tcagctcttg tcgcatagat tagtccttta agtccttgtt
    17341 ctttatatga cttattccac ctataaaggg tgcttaacgc aatcccttta cttttagaaa
    17401 tacttgataa agattgttcg cctaggataa aaggttttat aatattgtac ttttgagtag
    17461 cttttttacg tttagcttca gaaaaattag ttagatactt ctccttgttt ttcatcttcc
    17521 ttgtcctcca atttattgag gtaacgataa attgttgtac gtgaaacatg ccactgttct
    17581 gcaattgtct taattggcgt gccattatca taaagggtct taagtaagtt taaatctttt
    17641 tgatttaatt tttctggtct gcctccatat cggcctctag cacgtgctgc aattcggcca
    17701 gctgaagaac gctctaaaat taaattacgt tcaaattctg caaatgctgc gaataaatga
    17761 aataataatt gtccagttga acttgattta tccattgtta tattttcttc taaactatgg
    17821 aaacttacac ctcgttcgtt aagctcatta actaatgtga ttaaatcctc catattacgt
    17881 cccaatctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa ttcaattgct
    17941 ttatctaaac caggtctttt actttttgaa ccactcatat ggtcattaaa tatcttttca
    18001 caaccatatg tatttaatcg atcctcctgt aaatttaaat tttgtaatcc agttgaaact
    18061 cttgcataac ctattttcaa aatacaaacc tcctatatac atcttcagtg taccataact
    18121 taattacaag gtcttttatg aacatagatt ttttgttggg tttttgaaca cataatccta
    18181 tgtaattctt acttatttag acataaagaa gagtgtacag aaaatggtta ttttctgtac
    18241 actctcatct ttaataatat attttaaatg tcattaacaa aactaattta ataagagtca
    18301 agcatagttt acatatgtat tatgtttgaa acatacctct aattactttt tactaatatc
    18361 atttaaaata tctcgcaatt cttcaatttc tttgttattt aattcttcat ttttcgcaaa
    18421 attcaacact aaacttttca tatcccctcc atacagttta ttaagaaagg ttttagcagt
    18481 tttcatttta atatcgtctt ctttaatatt tgatgagtaa aaataaatat tttctgattt
    18541 gtatcgtttt ataatctctt ttttatatag tcttgtgatt aatgttctaa tcgttttatc
    18601 actaacttct ttatattttt gaatttcaac tacaatttca ttagctgata ctgatttttt
    18661 atcccatatt atattcataa catcccattc agccatagat atttcaactt gcttattggc
    18721 catttaaaac acccattcct tttaatattt tttcacttat taattcagca tttttcccag
    18781 acgggtttcc atctgataaa tgtgtagcaa aataatactt atcatgattt gtaattacgt
    18841 aacctacaaa ccacccatta ttatacttcc cgtttactat acctgtacct gttttcccat
    18901 acagttcata cttttcattt ttcttaatca ataatgaaga agataattga ttctttgctt
    18961 ttttactaaa atggttattt tgttccatca tatttttaaa aactattact tgttcaagat
    19021 tagatatttt caaactatct tccatccaat agcttttata acttcccaaa ttttcattac
    19081 cataatttaa ttgcttgagt tgagccgcag tatagttctt tggtatttga ttactaatac
    19141 gttcgaagta ccaattaact gaattttgca ttgctgtatt taaatcttgt tccttattcc
    19201 aagactcgaa aagataatgt ttatgattcc atgacatacg tgaattttta tcacttataa
    19261 tatgacggtc aagtccaaac agtgctaaat aaattttgta agttgaatca ggagaatatc
    19321 tttttctact ttctttttca ttataaatat agtatttgtc tttcttcatg ctgtacataa
    19381 cgaaagatcc actatttgaa ccaaaatttt tactttcatc taaaatttgg taatcgcttt
    19441 gtaagggctt tttataatta taatcagtta aggattgccc cattaaaaac tggctttgaa
    19501 ttatcatgat aaaaaagtaa aaatacaaat aaaaattaga attaattttg actgcttttt
    19561 cagatcggct tcttttatat taattaatct atttttgagt aatgattttt taccattaaa
    19621 tgaatggctt aatatatttt tgttgaaaaa tgaggttttt aatacggagt ccataataga
    19681 ttccgcataa gttttaaatt catttttgtt aatattatta aggacaaatc tatcggcttc
    19741 tacttcattg tcatgaatta tctttctttt tacaatatgt actaatggat tgtaactcat
    19801 aataatagag aagatattaa agataattaa atgtaaagta tctctatttt tagcatgagc
    19861 atattcatgc agtattatat attttagtct tttatcaatt acacttttaa aatatgaact
    19921 aggaatcaaa ataatatatt tcccatacca aaaagttatt ggagattgaa tagcctctgc
    19981 ttttcgaatc acaatatttt ttatattgat ggttgaaaag tatcgtatct attttatttt
    20041 ttcattttcg tttagataaa gtgactgttt ctttaaatat ttaagatata ataaggcttt
    20101 caaaaattta aaacttaaaa taataactaa aactatccaa ataactgtgc agatattatc
    20161 aattgaatcc caattaaact tatggatatc tgttgcgaac tcttgaacag gtttggtggt
    20221 aattatgtta tggttcaagt cgtgtgactt actttcaact gtgggcgctt gattattcac
    20281 attattaaat tttataaaag agaatttaat tgggataaaa ggaattaatc cagcaagcac
    20341 agttagatac caaactttat aatttaaact ataattaaaa tagcgtttta atatgtacct
    20401 aaaaaatact aacaatagaa atataaaaca aaagcttact acactcatta ttagtaattt
    20461 agccattttg acaccttctt tcaaatattt ataataaaca attgacatca atattacaat
    20521 tgtaatatta ttgttttgta aaaattacaa ctgtaatatc ggagggttta ttttgaaaaa
    20581 gttaatattt ttaattgtaa tggctttagt tttaagtgca tgtaattcaa acaattcaca
    20641 tgccaaagag ttaaatgatt tagaaaaaat ataatgctca tattggtgtt t
//
