LOCUS       CP139235                9730 bp    DNA     circular BCT 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain ARLG-3484 plasmid pARLG-3484-4,
            complete sequence.
ACCESSION   CP139235
VERSION     CP139235.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868158
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Genomic characteristics of 6 CR-HvKP strains in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/28/2023 20:13:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,869
            CDSs (total)                      :: 5,746
            Genes (coding)                    :: 5,571
            CDSs (with protein)               :: 5,571
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 72 of 175
            Pseudo Genes (incomplete)         :: 107 of 175
            Pseudo Genes (internal stop)      :: 24 of 175
            Pseudo Genes (multiple problems)  :: 27 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3484"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pARLG-3484-4"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            join(9025..9730,1..1484)
                     /locus_tag="JJW40_29270"
     CDS             join(9025..9730,1..1484)
                     /locus_tag="JJW40_29270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_function:
                     GO:0015343 - siderophore-iron transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0015891 -
                     siderophore transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WPQ29325.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            3070..5031
                     /locus_tag="JJW40_29275"
     CDS             3070..5031
                     /locus_tag="JJW40_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WPQ29326.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5031..5762
                     /gene="mobC"
                     /locus_tag="JJW40_29280"
     CDS             5031..5762
                     /gene="mobC"
                     /locus_tag="JJW40_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WPQ29318.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5769..6299
                     /locus_tag="JJW40_29285"
     CDS             5769..6299
                     /locus_tag="JJW40_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ29319.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6327..6506)
                     /locus_tag="JJW40_29290"
     CDS             complement(6327..6506)
                     /locus_tag="JJW40_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WPQ29320.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6575..6952
                     /locus_tag="JJW40_29295"
     CDS             6575..6952
                     /locus_tag="JJW40_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WPQ29321.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(7014..7313)
                     /locus_tag="JJW40_29300"
     CDS             complement(7014..7313)
                     /locus_tag="JJW40_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WPQ29322.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7303..7566)
                     /locus_tag="JJW40_29305"
     CDS             complement(7303..7566)
                     /locus_tag="JJW40_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WPQ29323.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            7943..9028
                     /locus_tag="JJW40_29310"
     CDS             7943..9028
                     /locus_tag="JJW40_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WPQ29324.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     Typing          complement(1690..2270)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5032..5759
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 acgtcattta cggaattatg gaggcagatc tgactgataa taccctactt acgattgggg
       61 gatatcagca gtatgaatat aacaatcatt ggtggtatga cttacctatt tctggtaccg
      121 gacaccacct taatcttcct cgctcgacgt ttacaggcaa tgactgggag tttgaaagaa
      181 gtaaaagtaa aactgcattt gcaactctgg agcagaagtt cagtaatagc tggaagctga
      241 aattttcaac gctgcaaaac tggagaaatg ctgacctgtt agggacggca acataccggc
      301 ataaagatac ccgacaaagt atgtatcagc aagtatgggg atcactgagg gaatataatt
      361 ccaaaagcta tgacctgtcg cttaatgggg actatgcttt atttggtcgt gttcatcaat
      421 tcgtggctgg aacaactcag caaaaataca ccgataaaga tacaggttat acatattccc
      481 caatatatat tgagaatgtt aatatatttg atcatggttc acattcttcg aaaaaaccca
      541 tgaagatgtc aggtgatgca tcctacgacg tcactgaaca aaaaagcgcc tacgcagcaa
      601 ctcattttct acttagtgat aactggaaaa tattgctggg tggccgtctt gactggtatg
      661 aatatacaag taaaggtaca tccattaata caggttataa agttaccaga aatatcacca
      721 aatatgcagg ttcaacatac aatcttgacg atcatcatac catttatggg agctataccg
      781 atatattcaa tccacagtca agcaccgacc tcaatggtaa agtgatcaag cctattgttg
      841 gtaagaatta tgagacaggt attaagggtg agtattttaa cggcttgctt aatgcctctc
      901 tggcggtatt tcaggtagac caggaaaatc gagccaggct gcttcccgat cagacagggt
      961 gtgttactaa tggcaatcct caatgctatg aggctagcgg taaagtacgc acaaaaggga
     1021 tagatttcga acttcagggc atggttacgc cactctggga aattggggtg ggatatacat
     1081 atgcagatac gcgatatata aaagatggag atcgtagtaa tgaaggcaag cgattcaata
     1141 ctgccgtgtc gccacaaaat ttatttaaga tttcgagcat gtacaccttt gacggaaaat
     1201 taacaaactg gcgagcagga gtaaacacgt actggcaaag taaaatatat gctaacataa
     1261 cagacacaag ggatggcaat ccggttatta ataaacaaaa ggcttattca acaactgaca
     1321 ttattattgg ctatactcca atgccacaac tggatttaca gctcaatata actaatgtct
     1381 ttgataaggt ttattataca tccataggtg aatcgattgg atatggcacg gaaacatatg
     1441 gagaaccaag aaatgttaaa attagtgcaa cctatagatt ctaaccatgc ggattgggtc
     1501 ttctcctcgg atttgcccct ataaatccgt cttttgtggt tccggcgtac aaccccctgt
     1561 ttgccacttc agttgatggt taatcaaatc atgtagagga tcttcttgag atcctccatg
     1621 gttcgtcgta atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga
     1681 aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa
     1741 ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt
     1801 ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt
     1861 tgcgtgtctt tccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg
     1921 ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag
     1981 ataccagtgt gtgagctatg agaaagcgcc acacttcccg taagggagaa aggcggaaca
     2041 ggtatccggt aaacggcagg gtcggaacag gagagcgcaa gagggagcga cccgccggaa
     2101 acggtgggga tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc
     2161 ctcacggctc ccacagatgc accgaaaaag cgtctgttta tgtgaattct ggcaggaggg
     2221 cgaagcctat ggaaaaacgc caccggcgcg gccctgccgt tttgcctcac atgttagtcc
     2281 cctgcttatc cacggaatct gtgggtaact ttgcgagagt tagcagcgcc cgccgcagtc
     2341 tcacggccgg agcgtagcga ctgagtgagg gaggaggcgc aatatccccc ctcccgcaga
     2401 cggccaccgg acgcgcggat gtgacatatt gcaccgtgtt accgcctttc ccttccctgc
     2461 cgataaatca ttcagatagg tgaagtctgt cggttttctg ctgaatattt tacgttaacc
     2521 tatctctgtt gcttttttct ccttgcggag aaatgcaggt catccccgct ggggcgtggc
     2581 tgacttcagg aggaaatccc ctgcttacgc tggctggacg ccaccgacgc acggtctttc
     2641 cccctctttc tggctcagct cggctggacg ccggccacgc cagaaattca cccggccccc
     2701 gtcaggctca cgcctgacca cccacaaaaa cccacggtgc tgaacgcacc tgacgaagca
     2761 gcaaagctgc tcctgatttt gcgggtggtc agccgctcgc tatggcgaac tctgacgggg
     2821 gccggaacac cggaaaagac cgcactgggg tgccgccttt tcctctcaca cgctggggcg
     2881 tgctcgttgt tcctccgggg ctgcgccttt tccgcttggg gcggaaagag gctggcctcc
     2941 gtggtgaaag ctgaatgcaa tcaccacgga cgtgaccgcc gccgccatgc ctcggggcat
     3001 gtccgcggct tgtgcagcag gggctgcaca ccacgccggt aaacccgtcg tctgaaggag
     3061 agagattgca tgtttaatac ggattcgctt gcctggcagt ggcatatccc ggagctggcc
     3121 cggcagattt acccgtgggt cagctggctg tacagggagt attacggtct ggccggggct
     3181 ttcctggtta tcggcctgtt tttcggtctg gctgaactct gggccggacg gatgcgtttt
     3241 gtggggctga gtcataagga gcgacgggag tatgccgatc gctataagcg cgataataac
     3301 cggactgcgc cattttcggt gctggcttcg ctggattcgc ccgggcggat ggtcagggca
     3361 cggcggcatg tctggaacta cgcgtactgg ctgaccggcg ctgccggtct ggcccttctg
     3421 ggggggctgg ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgtccggac
     3481 ctctacgcga acgcagatta ttacctgctg cagccggcta acctgttcgg gattgtctgg
     3541 tgtgccggag ggcttgtcgc cggcatcctt ctggcgcggc tgctggcatt tctgctgctt
     3601 gacggtcatt ttgcggccgc tgacgaggcg gtgaatgcga aactgaacca ggagagccgg
     3661 cgcagcagcc agcggaccgg agaaatgacc gacgtccgcc acctgcattt cggggagccg
     3721 gtaccggtta acgcgctggc cgatttttcc acagagcagg cccggaaaca gcaggcggtg
     3781 tttctgggta aggatgagca gggccagccg gtgctggtcc cgcgtgatac ctggcgcaag
     3841 accaatattc agatcctcgg cctgcccggg agcggcaaga gcgtgatggg caccaacgcg
     3901 ctcattcgct gcgtacgcga tttcggggac gcggtggtgt acttcgaccc caaaggggac
     3961 gcgtgggcgc cgcacgtctt ccgggcgcac tgcccggact tcaccctgct ggatctgcgg
     4021 ccggggaaac cggcgcagct gaatctgttt cgcgatctcg accagtacgc cctgaagaat
     4081 ctgctggttg ccgggtttaa cctgagtgag accggcgacg tggcggacca ctaccgcatc
     4141 agcgagcaga aagcggcgaa gctgattgcg gagcagttcc cgcagggggc aaacattcag
     4201 caggtgctgg cggcggcata cgcgctgccg gaggccctga aaaaggacgt gaaggggctg
     4261 atcaccaagc tggagaacgt ggccgacctg agcgtcctgc agacggacag cggcatcgac
     4321 gtggcgggga taatcaacgg cggcggctgc ctgtacgtca tcggctcgat ggacgatgag
     4381 gcggtgatcc gggtgcagaa gatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg
     4441 gatgagttcc ggaggtggcc gcacgcgagc atcatgctcg atgagattaa atacctgctg
     4501 tcgaagtacg tgctgaacgc gctggggacg ctgcgcagtc gggactgcaa cctgctgctc
     4561 gcgcaccagt cgctgggcga cttcgggcag tgcggccagg atctgcccgc tgagtttgtg
     4621 aaaaccacgg tgctggataa cacgccgatc cgctggttct accgggcggc cagccaggag
     4681 tccgcgcagt gggcggcggg ccagaccggg gaaatccggg tggacgtgga gcgccgccgg
     4741 gccagccggg aggcggggaa cgtggagcat atcagcgggg acagcttcat tcagaaggag
     4801 gcgcggccgc tgtttgacgt gaacaccctg cagcacctgc ctgacgggtt tgcggtgatg
     4861 accgggctgg gcgtggcccg gatggcgttc agcagcccgc tgcgggttga acgccgggaa
     4921 atcccgctgc agcgcttccc ggtgctggcg aaaacggacc cgctggcgga gtaccagccg
     4981 gaggccgggc ggcagcggcc gggtgatgat gattttgcgg ggctgtactg atggcactgg
     5041 agcgatacaa cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acccggctga
     5101 ccctgcgctg gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg
     5161 ggattgcggc ggtgcagccg gtttaccgct tcctggacag cctggtggcg aaagggctgc
     5221 tggtccgggc gaaatacccg gttgacggcc gtcaggtcag cgtgtgggga ctgacgccgc
     5281 acggggtggc gttctcgttc gacgaggacg aaccgctgac ggacgttatt ccgtttcagc
     5341 cgtcaagggt gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgtctcg
     5401 ccatggaggc ccggggggcc tccggctggc ggtacctgca ccgtatggcc ctgaagggga
     5461 tgaaggtgcc ggacgcgctg gcggagctgg acggcagaac ggtggcgttt gaggtggagc
     5521 gcaccgtgaa gagccgcagg cgctatcagg aggtggtgtc cgggtatctg ttcaatcgca
     5581 aagcgaacgg catcgatgaa atctggtaca tctgcccgga ccgggcgacg caggtcaggg
     5641 tgcagcgggc gatcctgtcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca
     5701 aaacggcgga gctggaccgg gagcggctgt ttgcgtgctt taaatttatg acaacggagt
     5761 gagggattat ggcgctgtac atggtattta ttgaagacgc ggaggaccgg gacggcgagc
     5821 gctgcatcat gccgaccagc gagcatcttg aagatatggt ggtgctcgcc tggcagaccg
     5881 gggcgtatgt tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg
     5941 ggatgtatga cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtaaaa
     6001 atccgcaatg ggaacgggac aaacccgcag gaatgaagct gtgtgattac ctctatcgca
     6061 aggtggagcg gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac
     6121 cggagcagat tagcgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga
     6181 acgagaagct gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg
     6241 tgcctgaact gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataaa
     6301 cggcagcacc ggccatgccg gtgctgttat tccttcccga gcttatcccg catcacctgc
     6361 cagagtgttt ccgcatcctc gtgcagccgc tcgcacatgt cggccggggt atccatatcc
     6421 agctcgttca gtttctcgag taacagcagc gactggtcct taataaattt agccatattg
     6481 agggcagttt tttcctgctt agtcatgttt tctgtccttc cgggtgtggt gtgcttcctg
     6541 tcgggtatac tgtctcaaaa ccggaggtga gacaatggcg tgggtgacgg tgagacaggc
     6601 gacggaactg acggggaaag cccggagttc cctgtatagg gatatggcga aaggccgcgt
     6661 atcataccgt actgaagctg atggtggtcg ggtgatggat accagcgaac ttatccgggt
     6721 gtacggtgaa ttaagacaga ttgagactca cgagcgggac gggttgagac tgtcggatga
     6781 gactgaaaaa atggcctccg acgcgttgat cgcggagata aaagcactca gggaggaggt
     6841 ggccggactg cggcaggaaa tgcaggccat gagactgctg gaacacaaac cagagacagc
     6901 agagacaccg gccggaacat cgcgccgatg gtggcagtgg ggaaaacgct gacgcgctgt
     6961 gcttgtccaa ccggcgacgg ccacgcaatg aattgcgccc ccatcaccgg ttatcagcga
     7021 acggtatggg gattctggcg ctggccaacc acggccagaa cccggacttt cctggtctgg
     7081 tcatcaacat catagaggac atgctgtccc atcagactga tccggcgaac gtcctcgcca
     7141 tagcgtgagt ccacttcata taggcgcggg aagtcggcca gtttgtccgc tgcagcgaga
     7201 actcgatcgg taacgttctg cgcatcccac agggatgaat tttccgcaat gtagtcaaac
     7261 aatgctgtca catcctcgat ggctccctgc ttccattcca cctcatacgg catcgcgtag
     7321 ttcctgatag cggccatgca gtctctggcg gagttgttcc gtggtcatgc ccgggcgcgt
     7381 gtcagcgcgg gcggcggtca ctttagcgcg aacctgatct tcatattctg cctgggccag
     7441 ttcttcgtta gccaggcgct gcaacagtgc agctttgtcc gggccacctt caggcagtat
     7501 gcggggcagt ctctcggcca gttcccgcag gaaattggcg ttttcatagt gtgtactgtt
     7561 gctcattgtg gagcctcccg ggctgatacg tcgatatgaa ggagtttagc agggggcggt
     7621 ctgaaagaat atgctcgtgt cagtcaggct gatgaagacg aaagctggta aaaaagggct
     7681 tacgacaccc attttacgtc aaactgcacg acatgcagtt gcgtgcatag ccatgcagcg
     7741 ggttaaaatt tatcaggcgc gtttccggcg gttttccggg tggtttgttg ccggttttat
     7801 cccgtagccg ccggaaatgc cctcagaccg tctgagcggt gcgcgtaatg cgacgttatg
     7861 gtaaatcatg tgattaatgg tagtgataat cattatcact tttgctatat tcttgtttct
     7921 tttattgtaa ggaaatgtaa gtatggcaat cacgattaaa tccgccgtta atgtccgtag
     7981 cagagtatta atatactctt tactactgtt tactggttca gcgtatgcgg ctgatatagc
     8041 tgaatcaaca ttcctgactc gtgatatcgg caatggtctg tatgaaatgt cttttgatgg
     8101 caaaaataat agtctctggg cagcatcatc gtcttcattc gacagagata agggccacgg
     8161 aattgtttat cagttagaac catccactct gaaaacaaaa tctgccgtta aaatcgcacg
     8221 tcttgggttt gccacagcac tggatgagga acatgagcga ctttatatcg gcaatactct
     8281 ggacggctca gtgacggtca ttgatactct aaccggaaaa gaactttcag taattccgtt
     8341 aactgaggga caggatgagg ctcatttcag accgaccaga gaaatggtca ttgacaaaaa
     8401 aaatcagcgt ctttatgtct ccagcgccgc agataaaggg gctctgtggg ttattgacat
     8461 ggctgtcggg aagaaaatta aaaccattga tggactgggg cacgctgtta ctggaattgc
     8521 gttaaataaa aatggggata atatttatct ggttaacggg gatggtgaga ttattaacct
     8581 ggatgccagt agctatgaaa taaaaaaacg gtttgttgta gagcctgaga aaaaacactt
     8641 tttccttaat gtaagtctgg atgaaaaaaa cggacgtgca tttattaccg acccggatat
     8701 tcccggggtg ctggttgttg atgtaaactc gggaaaaata ctacacagaa ttgatgttat
     8761 aaattcactg gcgattttat ttaatccttt acggaacgaa atctatatca cgcaccgtaa
     8821 tgcaaaacag gtcagcattg tggacagtaa ttcttattac gtaaaaacaa cgataaaaac
     8881 ccgcctgatg ccaaacagtc tgtctttatc acatgacggt gaaaatctgt acgttagtgt
     8941 aaaacaagaa aaaaaagaga tgagtactat gaaggattac atactcaaaa tcgaattatc
     9001 taaaattgct atggagccag aagaatgaat aaatatttag ggctgagcat agtatatatg
     9061 atgtatacat catctgttat ggctgatgat tctggcgcag ttagtaaaga taaaaatatt
     9121 tcaccagaag aaacattaat tgttcatggg accgctgttt ctgaaagcac aacagaagga
     9181 acagattcat acacaaccca gtcagccaat acagcatcaa agatgaatct gtcattccgg
     9241 gatacacctc agtcaactac agtcattacg cgacgaagaa tggatgatgt agcagcaaaa
     9301 aatattattg atgtggttaa aaccgctcct ggattatatg tcagtacagc agaaggttcc
     9361 ggaagaccga attttatgtc cagaggattt aatgctaatg tcatgtatga aggatttagc
     9421 ctgccgtgga attcatacat tccaagttca caaactagtt cagctatttt tgaccgggtg
     9481 gagatagtcc gcggggcaac aggtcttgtt cagggtgccg gagacccttc agccgcgata
     9541 aatatgatac acaagcgtcc gacccggcaa tatcaggcca gcactaacat cggtgccgga
     9601 cggtggaatg actaccgggg tggggttgat gtatcaggac cactgaatga cagcgcgacc
     9661 ttacgcggaa gggcagttgt ggatggcgaa aacagtgaga ccttcaggga tagtgagaaa
     9721 cgcaggaata
//
