LOCUS       CP139229                9730 bp    DNA     circular BCT 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain ARLG-4720 plasmid pARLG-4720-4,
            complete sequence.
ACCESSION   CP139229
VERSION     CP139229.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868726
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Genomic characteristics of 6 CR-HvKP strains in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/28/2023 20:20:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,847
            CDSs (total)                      :: 5,719
            Genes (coding)                    :: 5,541
            CDSs (with protein)               :: 5,541
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 75 of 178
            Pseudo Genes (incomplete)         :: 107 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 27 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4720"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pARLG-4720-4"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            join(8443..9730,1..674)
                     /locus_tag="JJ203_29160"
     CDS             join(8443..9730,1..674)
                     /locus_tag="JJ203_29160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WPQ23747.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            674..1405
                     /gene="mobC"
                     /locus_tag="JJ203_29165"
     CDS             674..1405
                     /gene="mobC"
                     /locus_tag="JJ203_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WPQ23748.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1412..1942
                     /locus_tag="JJ203_29170"
     CDS             1412..1942
                     /locus_tag="JJ203_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ23749.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1970..2149)
                     /locus_tag="JJ203_29175"
     CDS             complement(1970..2149)
                     /locus_tag="JJ203_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WPQ23750.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2218..2595
                     /locus_tag="JJ203_29180"
     CDS             2218..2595
                     /locus_tag="JJ203_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WPQ23751.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(2657..2956)
                     /locus_tag="JJ203_29185"
     CDS             complement(2657..2956)
                     /locus_tag="JJ203_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WPQ23752.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2946..3209)
                     /locus_tag="JJ203_29190"
     CDS             complement(2946..3209)
                     /locus_tag="JJ203_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WPQ23753.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            3586..4671
                     /locus_tag="JJ203_29195"
     CDS             3586..4671
                     /locus_tag="JJ203_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WPQ23754.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            4668..6857
                     /locus_tag="JJ203_29200"
     CDS             4668..6857
                     /locus_tag="JJ203_29200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_function:
                     GO:0015343 - siderophore-iron transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0015891 -
                     siderophore transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WPQ23755.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(7063..7643)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          675..1402
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 tcatcggctc gatggacgat gaggcggtga tccgggtgca gaagatgctg tttgcccgct
       61 gcgcgcaaat catcattgcg cgggatgagt tccggaggtg gccgcacgcg agcatcatgc
      121 tcgatgagat taaatacctg ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca
      181 gtcgggactg caacctgctg ctcgcgcacc agtcgctggg cgacttcggg cagtgcggcc
      241 aggatctgcc cgctgagttt gtgaaaacca cggtgctgga taacacgccg atccgctggt
      301 tctaccgggc ggccagccag gagtccgcgc agtgggcggc gggccagacc ggggaaatcc
      361 gggtggacgt ggagcgccgc cgggccagcc gggaggcggg gaacgtggag catatcagcg
      421 gggacagctt cattcagaag gaggcgcggc cgctgtttga cgtgaacacc ctgcagcacc
      481 tgcctgacgg gtttgcggtg atgaccgggc tgggcgtggc ccggatggcg ttcagcagcc
      541 cgctgcgggt tgaacgccgg gaaatcccgc tgcagcgctt cccggtgctg gcgaaaacgg
      601 acccgctggc ggagtaccag ccggaggccg ggcggcagcg gccgggtgat gatgattttg
      661 cggggctgta ctgatggcac tggagcgata caacgtcagc cacgcgaagc ggcaggcgcg
      721 gaacgcggag aaaacccggc tgaccctgcg ctggctgcgg gaagagctgt gttccacggc
      781 ggagctggtt gcgcgacggc tggggattgc ggcggtgcag ccggtttacc gcttcctgga
      841 cagcctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg gccgtcaggt
      901 cagcgtgtgg ggactgacgc cgcacggggt ggcgttctcg ttcgacgagg acgaaccgct
      961 gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc cgcaccggct
     1021 ggccgtgcag tccctgcgtc tcgccatgga ggcccggggg gcctccggct ggcggtacct
     1081 gcaccgtatg gccctgaagg ggatgaaggt gccggacgcg ctggcggagc tggacggcag
     1141 aacggtggcg tttgaggtgg agcgcaccgt gaagagccgc aggcgctatc aggaggtggt
     1201 gtccgggtat ctgttcaatc gcaaagcgaa cggcatcgat gaaatctggt acatctgccc
     1261 ggaccgggcg acgcaggtca gggtgcagcg ggcgatcctg tcggtggatg aaatcgtgaa
     1321 tccacagacc ggcgaggccc gcaaaacggc ggagctggac cgggagcggc tgtttgcgtg
     1381 ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgaaga
     1441 cgcggaggac cgggacggcg agcgctgcat catgccgacc agcgagcatc ttgaagatat
     1501 ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca
     1561 tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc
     1621 cacgtacttt ttcaccggta aaaatccgca atgggaacgg gacaaacccg caggaatgaa
     1681 gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt
     1741 ggttgatgcc gggaagtgct caccggagca gattagcgcg atgattgagg tggtcagcga
     1801 gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa
     1861 cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg cccgccggtg
     1921 gtggccgttc ggcaaaaaat aaacggcagc accggccatg ccggtgctgt tattccttcc
     1981 cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgcagc cgctcgcaca
     2041 tgtcggccgg ggtatccata tccagctcgt tcagtttctc gagtaacagc agcgactggt
     2101 ccttaataaa tttagccata ttgagggcag ttttttcctg cttagtcatg ttttctgtcc
     2161 ttccgggtgt ggtgtgcttc ctgtcgggta tactgtctca aaaccggagg tgagacaatg
     2221 gcgtgggtga cggtgagaca ggcgacggaa ctgacgggga aagcccggag ttccctgtat
     2281 agggatatgg cgaaaggccg cgtatcatac cgtactgaag ctgatggtgg tcgggtgatg
     2341 gataccagcg aacttatccg ggtgtacggt gaattaagac agattgagac tcacgagcgg
     2401 gacgggttga gactgtcgga tgagactgaa aaaatggcct ccgacgcgtt gatcgcggag
     2461 ataaaagcac tcagggagga ggtggccgga ctgcggcagg aaatgcaggc catgagactg
     2521 ctggaacaca aaccagagac agcagagaca ccggccggaa catcgcgccg atggtggcag
     2581 tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga cggccacgca atgaattgcg
     2641 cccccatcac cggttatcag cgaacggtat ggggattctg gcgctggcca accacggcca
     2701 gaacccggac tttcctggtc tggtcatcaa catcatagag gacatgctgt cccatcagac
     2761 tgatccggcg aacgtcctcg ccatagcgtg agtccacttc atataggcgc gggaagtcgg
     2821 ccagtttgtc cgctgcagcg agaactcgat cggtaacgtt ctgcgcatcc cacagggatg
     2881 aattttccgc aatgtagtca aacaatgctg tcacatcctc gatggctccc tgcttccatt
     2941 ccacctcata cggcatcgcg tagttcctga tagcggccat gcagtctctg gcggagttgt
     3001 tccgtggtca tgcccgggcg cgtgtcagcg cgggcggcgg tcactttagc gcgaacctga
     3061 tcttcatatt ctgcctgggc cagttcttcg ttagccaggc gctgcaacag tgcagctttg
     3121 tccgggccac cttcaggcag tatgcggggc agtctctcgg ccagttcccg caggaaattg
     3181 gcgttttcat agtgtgtact gttgctcatt gtggagcctc ccgggctgat acgtcgatat
     3241 gaaggagttt agcagggggc ggtctgaaag aatatgctcg tgtcagtcag gctgatgaag
     3301 acgaaagctg gtaaaaaagg gcttacgaca cccattttac gtcaaactgc acgacatgca
     3361 gttgcgtgca tagccatgca gcgggttaaa atttatcagg cgcgtttccg gcggttttcc
     3421 gggtggtttg ttgccggttt tatcccgtag ccgccggaaa tgccctcaga ccgtctgagc
     3481 ggtgcgcgta atgcgacgtt atggtaaatc atgtgattaa tggtagtgat aatcattatc
     3541 acttttgcta tattcttgtt tcttttattg taaggaaatg taagtatggc aatcacgatt
     3601 aaatccgccg ttaatgtccg tagcagagta ttaatatact ctttactact gtttactggt
     3661 tcagcgtatg cggctgatat agctgaatca acattcctga ctcgtgatat cggcaatggt
     3721 ctgtatgaaa tgtcttttga tggcaaaaat aatagtctct gggcagcatc atcgtcttca
     3781 ttcgacagag ataagggcca cggaattgtt tatcagttag aaccatccac tctgaaaaca
     3841 aaatctgccg ttaaaatcgc acgtcttggg tttgccacag cactggatga ggaacatgag
     3901 cgactttata tcggcaatac tctggacggc tcagtgacgg tcattgatac tctaaccgga
     3961 aaagaacttt cagtaattcc gttaactgag ggacaggatg aggctcattt cagaccgacc
     4021 agagaaatgg tcattgacaa aaaaaatcag cgtctttatg tctccagcgc cgcagataaa
     4081 ggggctctgt gggttattga catggctgtc gggaagaaaa ttaaaaccat tgatggactg
     4141 gggcacgctg ttactggaat tgcgttaaat aaaaatgggg ataatattta tctggttaac
     4201 ggggatggtg agattattaa cctggatgcc agtagctatg aaataaaaaa acggtttgtt
     4261 gtagagcctg agaaaaaaca ctttttcctt aatgtaagtc tggatgaaaa aaacggacgt
     4321 gcatttatta ccgacccgga tattcccggg gtgctggttg ttgatgtaaa ctcgggaaaa
     4381 atactacaca gaattgatgt tataaattca ctggcgattt tatttaatcc tttacggaac
     4441 gaaatctata tcacgcaccg taatgcaaaa caggtcagca ttgtggacag taattcttat
     4501 tacgtaaaaa caacgataaa aacccgcctg atgccaaaca gtctgtcttt atcacatgac
     4561 ggtgaaaatc tgtacgttag tgtaaaacaa gaaaaaaaag agatgagtac tatgaaggat
     4621 tacatactca aaatcgaatt atctaaaatt gctatggagc cagaagaatg aataaatatt
     4681 tagggctgag catagtatat atgatgtata catcatctgt tatggctgat gattctggcg
     4741 cagttagtaa agataaaaat atttcaccag aagaaacatt aattgttcat gggaccgctg
     4801 tttctgaaag cacaacagaa ggaacagatt catacacaac ccagtcagcc aatacagcat
     4861 caaagatgaa tctgtcattc cgggatacac ctcagtcaac tacagtcatt acgcgacgaa
     4921 gaatggatga tgtagcagca aaaaatatta ttgatgtggt taaaaccgct cctggattat
     4981 atgtcagtac agcagaaggt tccggaagac cgaattttat gtccagagga tttaatgcta
     5041 atgtcatgta tgaaggattt agcctgccgt ggaattcata cattccaagt tcacaaacta
     5101 gttcagctat ttttgaccgg gtggagatag tccgcggggc aacaggtctt gttcagggtg
     5161 ccggagaccc ttcagccgcg ataaatatga tacacaagcg tccgacccgg caatatcagg
     5221 ccagcactaa catcggtgcc ggacggtgga atgactaccg gggtggggtt gatgtatcag
     5281 gaccactgaa tgacagcgcg accttacgcg gaagggcagt tgtggatggc gaaaacagtg
     5341 agaccttcag ggatagtgag aaacgcagga ataacgtcat ttacggaatt atggaggcag
     5401 atctgactga taatacccta cttacgattg ggggatatca gcagtatgaa tataacaatc
     5461 attggtggta tgacttacct atttctggta ccggacacca ccttaatctt cctcgctcga
     5521 cgtttacagg caatgactgg gagtttgaaa gaagtaaaag taaaactgca tttgcaactc
     5581 tggagcagaa gttcagtaat agctggaagc tgaaattttc aacgctgcaa aactggagaa
     5641 atgctgacct gttagggacg gcaacatacc ggcataaaga tacccgacaa agtatgtatc
     5701 agcaagtatg gggatcactg agggaatata attccaaaag ctatgacctg tcgcttaatg
     5761 gggactatgc tttatttggt cgtgttcatc aattcgtggc tggaacaact cagcaaaaat
     5821 acaccgataa agatacaggt tatacatatt ccccaatata tattgagaat gttaatatat
     5881 ttgatcatgg ttcacattct tcgaaaaaac ccatgaagat gtcaggtgat gcatcctacg
     5941 acgtcactga acaaaaaagc gcctacgcag caactcattt tctacttagt gataactgga
     6001 aaatattgct gggtggccgt cttgactggt atgaatatac aagtaaaggt acatccatta
     6061 atacaggtta taaagttacc agaaatatca ccaaatatgc aggttcaaca tacaatcttg
     6121 acgatcatca taccatttat gggagctata ccgatatatt caatccacag tcaagcaccg
     6181 acctcaatgg taaagtgatc aagcctattg ttggtaagaa ttatgagaca ggtattaagg
     6241 gtgagtattt taacggcttg cttaatgcct ctctggcggt atttcaggta gaccaggaaa
     6301 atcgagccag gctgcttccc gatcagacag ggtgtgttac taatggcaat cctcaatgct
     6361 atgaggctag cggtaaagta cgcacaaaag ggatagattt cgaacttcag ggcatggtta
     6421 cgccactctg ggaaattggg gtgggatata catatgcaga tacgcgatat ataaaagatg
     6481 gagatcgtag taatgaaggc aagcgattca atactgccgt gtcgccacaa aatttattta
     6541 agatttcgag catgtacacc tttgacggaa aattaacaaa ctggcgagca ggagtaaaca
     6601 cgtactggca aagtaaaata tatgctaaca taacagacac aagggatggc aatccggtta
     6661 ttaataaaca aaaggcttat tcaacaactg acattattat tggctatact ccaatgccac
     6721 aactggattt acagctcaat ataactaatg tctttgataa ggtttattat acatccatag
     6781 gtgaatcgat tggatatggc acggaaacat atggagaacc aagaaatgtt aaaattagtg
     6841 caacctatag attctaacca tgcggattgg gtcttctcct cggatttgcc cctataaatc
     6901 cgtcttttgt ggttccggcg tacaaccccc tgtttgccac ttcagttgat ggttaatcaa
     6961 atcatgtaga ggatcttctt gagatcctcc atggttcgtc gtaatctttt gccctgtaaa
     7021 cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg gaactgctta
     7081 accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca gtgtagccgg
     7141 actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc
     7201 ccagttacca atggctgctg ccagtggcgt ttttgcgtgt ctttccgggt tggactcaag
     7261 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc
     7321 cagcttggag cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc
     7381 gccacacttc ccgtaaggga gaaaggcgga acaggtatcc ggtaaacggc agggtcggaa
     7441 caggagagcg caagagggag cgacccgccg gaaacggtgg ggatctttaa gtcctgtcgg
     7501 gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa
     7561 aagcgtctgt ttatgtgaat tctggcagga gggcgaagcc tatggaaaaa cgccaccggc
     7621 gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta
     7681 actttgcgag agttagcagc gcccgccgca gtctcacggc cggagcgtag cgactgagtg
     7741 agggaggagg cgcaatatcc cccctcccgc agacggccac cggacgcgcg gatgtgacat
     7801 attgcaccgt gttaccgcct ttcccttccc tgccgataaa tcattcagat aggtgaagtc
     7861 tgtcggtttt ctgctgaata ttttacgtta acctatctct gttgcttttt tctccttgcg
     7921 gagaaatgca ggtcatcccc gctggggcgt ggctgacttc aggaggaaat cccctgctta
     7981 cgctggctgg acgccaccga cgcacggtct ttccccctct ttctggctca gctcggctgg
     8041 acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga ccacccacaa
     8101 aaacccacgg tgctgaacgc acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg
     8161 gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg
     8221 gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc
     8281 ttttccgctt ggggcggaaa gaggctggcc tccgtggtga aagctgaatg caatcaccac
     8341 ggacgtgacc gccgccgcca tgcctcgggg catgtccgcg gcttgtgcag caggggctgc
     8401 acaccacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa tacggattcg
     8461 cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg
     8521 ctgtacaggg agtattacgg tctggccggg gctttcctgg ttatcggcct gtttttcggt
     8581 ctggctgaac tctgggccgg acggatgcgt tttgtggggc tgagtcataa ggagcgacgg
     8641 gagtatgccg atcgctataa gcgcgataat aaccggactg cgccattttc ggtgctggct
     8701 tcgctggatt cgcccgggcg gatggtcagg gcacggcggc atgtctggaa ctacgcgtac
     8761 tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc
     8821 gagagctggg gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga ttattacctg
     8881 ctgcagccgg ctaacctgtt cgggattgtc tggtgtgccg gagggcttgt cgccggcatc
     8941 cttctggcgc ggctgctggc atttctgctg cttgacggtc attttgcggc cgctgacgag
     9001 gcggtgaatg cgaaactgaa ccaggagagc cggcgcagca gccagcggac cggagaaatg
     9061 accgacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct ggccgatttt
     9121 tccacagagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag
     9181 ccggtgctgg tcccgcgtga tacctggcgc aagaccaata ttcagatcct cggcctgccc
     9241 gggagcggca agagcgtgat gggcaccaac gcgctcattc gctgcgtacg cgatttcggg
     9301 gacgcggtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg
     9361 cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg
     9421 tttcgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt taacctgagt
     9481 gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt
     9541 gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc atacgcgctg
     9601 ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa cgtggccgac
     9661 ctgagcgtcc tgcagacgga cagcggcatc gacgtggcgg ggataatcaa cggcggcggc
     9721 tgcctgtacg
//
