LOCUS       CP139224                5596 bp    DNA     circular BCT 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain ARLG-7683 plasmid pARLG-7683-5,
            complete sequence.
ACCESSION   CP139224
VERSION     CP139224.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN19016597
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Genomic characteristics of 6 CR-HvKP strains in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/28/2023 20:07:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,833
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 74 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 24 of 177
            Pseudo Genes (multiple problems)  :: 32 of 177
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-7683"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pARLG-7683-5"
                     /geo_loc_name="USA"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            join(5442..5596,1..4)
                     /locus_tag="SOI88_29715"
     CDS             join(5442..5596,1..4)
                     /locus_tag="SOI88_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WPQ07351.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            362..688
                     /locus_tag="SOI88_29720"
     CDS             362..688
                     /locus_tag="SOI88_29720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07352.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            714..1259
                     /locus_tag="SOI88_29725"
     CDS             714..1259
                     /locus_tag="SOI88_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07353.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1325..1675
                     /locus_tag="SOI88_29730"
     CDS             1325..1675
                     /locus_tag="SOI88_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07354.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1745..2011)
                     /locus_tag="SOI88_29735"
     CDS             complement(1745..2011)
                     /locus_tag="SOI88_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WPQ07355.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1995..2219)
                     /locus_tag="SOI88_29740"
     CDS             complement(1995..2219)
                     /locus_tag="SOI88_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WPQ07356.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2886..3098)
                     /locus_tag="SOI88_29745"
     CDS             complement(2886..3098)
                     /locus_tag="SOI88_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WPQ07347.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3105..3614)
                     /locus_tag="SOI88_29750"
     CDS             complement(3105..3614)
                     /locus_tag="SOI88_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WPQ07348.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(3649..4353)
                     /locus_tag="SOI88_29755"
                     /pseudo=""
     CDS             complement(3649..4353)
                     /locus_tag="SOI88_29755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4292..4636)
                     /locus_tag="SOI88_29760"
     CDS             complement(4292..4636)
                     /locus_tag="SOI88_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WPQ07349.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4626..4802)
                     /locus_tag="SOI88_29765"
     CDS             complement(4626..4802)
                     /locus_tag="SOI88_29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07350.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(4889..4965)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          5154..5551
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2771..4302)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctaactgcgg ctacactgaa agaacagttt tggtatctgc ggtctcgtat acccagttac
       61 ccggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc caggcggttt
      121 tttcgtttac agtgcaagag attacgcgca gaaaaaaagg atctcaagaa gatcctttaa
      181 atgatcgtca gttctgttat ccccggagct ccatccatcc cacggaaaaa cacccgccac
      241 tgatgccctc agcgcactgc acatcatgta caaaccactc cctccctcct tttttattgc
      301 gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa acgcaatagc aagggggggt
      361 aatgagagaa agaacaaata tcatgctaga tataggatta aagcaatcct tacaacggct
      421 ttcgaataaa accaaaatga gtctaagtga tcttattaac cagagattat caggttcact
      481 ttcagttgaa gaacgcattg gcgaaaagcc tgaatggatg agaaaccttg atccctatct
      541 cgccaatcac ttttcaaata ttgagttacc gctcccttca ccattaaaag aataccaaga
      601 aaaaatggat gagatagatt caattattaa tgacatgatg gttctaaagg aatatttcag
      661 agctaagttt tacgacgaaa taaaataggc acgctatacg gaggcggaga attatgtttt
      721 ttaaacggta tttaacggag aaggaaatat acaaggcagc actcccaatt gttaggcttc
      781 aagggagagt taacacttca ttacgttttt tatccaaata ctcagatgta aacaaaaatg
      841 gattaattgc attgcatctt aatttattac attttatctc atacaacata cacggtgtag
      901 agtctctgaa aatcactcaa gcaggcataa acaaagctgt acaacaaagc cctcacgcag
      961 atgaaataaa aaatattgcg caggcaatag gacgcaaagg acctgagtac atcgaaggat
     1021 taattagaaa cccatggggg aagaaaaatg ataatccact aatatttata atgagcattg
     1081 aagaaagtat aaaagaaacc cttaactcag gttatatgtt atatagagta gctcccataa
     1141 ctgagggtga agcagctgct ttcatggatg ttcttaaaca attattatta tttgaggata
     1201 attttcgcct ttttggtaga gacttggttt ataaaataga aaaattaaaa atcagataac
     1261 aaagtatggt gaacgaaaaa tgaaagagag gatagtgaaa tgtgtaatac caagattctc
     1321 aactatgtat tggttaattg cgttcctagc atttatattc cccggtcgat cttttgctga
     1381 cggttacacc gcttactacc gatgtaatgg tactcaggaa gcatctctta ctgttaaaga
     1441 taacaaacta agtatggttg taggtctttc taagtttaat aggcaactgg atacttatag
     1501 attcaaagaa aagggaatcg aagtagtaat gcagagagct gcttctgatg ataactctga
     1561 ggccataatg tcttttatta tgcctgacgt taacaatatg accttattag ttaaaaataa
     1621 cggctacatt atcgttaaga caacctgctg gttaactaaa accgacaaac agtagaacac
     1681 gtagttagtc gagtatttac cataatacct gttgtcggca tcaaaaaagc ccgcatagcg
     1741 ggcttcagtc gtaaacttcg cggcgatgtc caatcgtggc ggcaaggata acaagctcgt
     1801 catctcggat ctcacacaga acgcgataat ctcccacccg atagcgccag aactcgccca
     1861 gctcaccttt gagcggcttc cctgactggc gaggatcggc agcaactgca atgcgtaaac
     1921 tcataaaatc cacaatccgt cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg
     1981 cccgatcgga atagttaatc gtccagacca agtgacttcc tcacgtcttc agacgaatgc
     2041 actgcttcat caccacggcg aacgcgtgca gcagtttctg ctgaaagata ataatcctcc
     2101 aggtcttcca gatgcgccag tattgcttcc cgcgcataaa acgtctttgt tctgccggtt
     2161 tgcttcgcca gcgagtccag acgggactca atctcatcag ataaccggat agcaagcata
     2221 taaacctccc gaacaccatt tgctatacat gtatatcaac agtaccacaa tcaacctaaa
     2281 agaacaaaaa ataatcttgt tagcccctct tgataatccg taacgcggtc aatatatcta
     2341 gcttgagggc tttcctctta actattgaaa ggataacgat tatgggtctt aagccgggtc
     2401 cgaaacgaat tgccgattca acaggggaac ctgataaacg ccagcgtgac aataaaaaaa
     2461 cgccgggaaa tactgacaaa ttgaaaccca gcaaatcttc aaaaaaataa gtcagtagtt
     2521 aaaagccctc cggggctttt ttgtatctgc cgttcacgct gattaaacat aacacccgtt
     2581 gcccgcaccg ccccggacgg gttcgccgcg aacggggtgg cagtcaggta aaaccggcaa
     2641 caaaccaccc gataaaccgc cccgttcgcg acagataaat tttacctggc tgcatagcca
     2701 tgcagccagg tgaatgacat ccggtttcag cgtaaatgcc cgatgagaac cctgttttcc
     2761 tgcatttcag cccagcgacg gaccgtcata gcttcgctgc cgggaacgcg cttccgtcag
     2821 ttccttttcg tgctgccgtt gctcgtgcgc ccgttctgcg gccacggcca tctcatgacg
     2881 ctgcactacc ggctcaaacg ctgcagttgc ccgctcaagc gttccacctg ctgactcaag
     2941 cgcgtcacgc gctcgctcag cgccgcgttc tcccgttgca taagccctga cattttccgc
     3001 cattccccga aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc
     3061 tctaatgcgc tcagcaactg tttttccagc tcggtcatcg gcacttactc cttcaccagt
     3121 ctcagcgcgt tctgcttcga ccgcccgttc ccgaccgtcc agctctgcgt cctgtctacg
     3181 gccgtcccct tcggcagcac cagaaaacgt ccgttgctgt cttccaggta cgtcacgccc
     3241 caggtcttcg cgttcagctt cgacagcgct gcgttctgcc ggtcgatttc ggccagattt
     3301 gacgctatca agctcccctg ataccacaac accccgctca ggctggcgaa cagcagtcct
     3361 gacaccatcg tgatggtcag ccatgtccgc ccgaccatac gcatcacgct caggcggttc
     3421 gactgcatgg ccgcgctcat accctgctcg tgggcgcgga tggcggcgct gattctcttc
     3481 ccgctttcgc tcagttccgc tctgacagac ttctcgtgct cgctgaatgc ggctttcagc
     3541 atttcgccgg tactctgctg ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt
     3601 aaaagactgt tcatagatgg ctcccttaag tcggatgttt cgccctccgt ccgggtcggc
     3661 aatgctgatg ctgctctttg tggtgcgcac gacctcaaaa cctgcgcttt ccagcgcctc
     3721 agtgacgtcc tgacgcgttt taagctcccc tgacgaggcg agggccagta agccccgcgt
     3781 aatagcctct gcggcttcct gcttcgtttt gggtaacgaa gatggcgtca ccagcgcgcg
     3841 ccggttctcc ggtgcgttcg ggtcgtgcag ccccagtcgc ccgttcacca cggtctgcca
     3901 cgcatcgatg cgcggacggt ctgcccggtc gtagtacggc tggagacgcc tgccggtcag
     3961 cagttccgtg ttcggtacca gaaaattcag ttccagccgc cccttgtccc ggtgttcaac
     4021 ccacagcacg ctgtactggt ctttatcgag tccgggcatc agaacccgct cgaagctcgc
     4081 catcagcttt tcacgctggc cgggcggtaa atcctgttca gcaaaagaca ggacgccgga
     4141 ggtgtacttt ttggcgtagg gcgaggcgtc gataagctcc cggacctcct ccggctttcc
     4201 ctgcaggacg atggccccgt cgcgctggcg gtctttcccc agcagataat ccaccggccc
     4261 ggcaccgccg cctcgccctc gcggatgaaa cttaacgatc atcgtctgcc cccttttcca
     4321 gcacggcatg ccgcagccgc tcgagttcgg catcaatggc catcagcgcg gccaccacct
     4381 gaacccggtc agcgcctgac cactggccgc cgttaatttt ccgggcgatc tggttgaggt
     4441 tgttccccat cccggcaagc tggcgcagca aaaccggatc gatggacggc agccgcaacg
     4501 accgcgccgg gcgcgtatcg agacaggttt cccgcatcca caccgccagt tgccttccat
     4561 tgcagcgctc cagcagctgc tggtactccc catcggtcac ccggatacct aaaaaacggg
     4621 tacgcttaac cgccatcctc tcctccgata tccgcaaaat gtgaccgggc tgaacgctca
     4681 agctctgcca tctgctcccg gcggtaatct tcctcctgtg cctgacgcat cagatttgcc
     4741 atcactgccg gatgccagga tctttttttt acgacgagcg gcttcttctt cggcttctgc
     4801 attatccctc cttcgcaaac gccggacggc acggaaaagt tttccgggtc gctccggcgt
     4861 tggacaagcg ccagcgcgtc agtggtgtta gcgggtttcg gggcgcagcc ctgaaccagt
     4921 cacagcgttt acgctgtgta tactggctta ctatgttggc atgagagcac gattcatgaa
     4981 agtgcctcat gtggcaggtg tgatatgtgg ctcaggagcg tccgcaatac catgcacgga
     5041 cagatgaaat gacgcctcct cgctcactcg gtagctgcgc tcctgccgtt cggctgcggc
     5101 gggcgtgacc gactcacgga taacgggata aagcaaaggc gctcaaatca gaaagggccg
     5161 gagaaaagga tgcggcgttg ccgtttttcc ataggctccg cctcccctga cgagcatcac
     5221 agaaatggac gctcaagtca gaggaggacg aaacccgaca ggacttaaag atgccggtga
     5281 tttctctccg gcggctccct cgtgcgctct cctgttccga ccctgccgtt taccggatac
     5341 ctgttccgcc tttttccctt tcgggaagcg tggcgctttc tcatagctca cgctgctggt
     5401 atctcggctc ggtttaggtc gttcgctcca agctgggctg tatgcacgaa ccccccgttc
     5461 agcccgactg ctgcgccctt tccggtaact atcatcttga gtccaacccg gacggacacg
     5521 caaaaacgcc actggcagca gccattggta actggatata tacgagagag atattgagag
     5581 gtcttgaagt gggtgc
//
