LOCUS       CP139223               11972 bp    DNA     circular BCT 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain ARLG-7683 plasmid pARLG-7683-4,
            complete sequence.
ACCESSION   CP139223
VERSION     CP139223.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN19016597
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Genomic characteristics of 6 CR-HvKP strains in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Jiang,J., Chen,L. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/28/2023 20:07:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,833
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 74 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 24 of 177
            Pseudo Genes (multiple problems)  :: 32 of 177
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-7683"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pARLG-7683-4"
                     /geo_loc_name="USA"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            complement(join(11262..11972,1..21))
                     /gene="mobC"
                     /locus_tag="SOI88_29640"
     CDS             complement(join(11262..11972,1..21))
                     /gene="mobC"
                     /locus_tag="SOI88_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WPQ07341.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(21..1985)
                     /locus_tag="SOI88_29645"
     CDS             complement(21..1985)
                     /locus_tag="SOI88_29645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WPQ07342.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3106..3256
                     /locus_tag="SOI88_29650"
                     /pseudo=""
     CDS             3106..3256
                     /locus_tag="SOI88_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3471..3620)
                     /locus_tag="SOI88_29655"
     CDS             complement(3471..3620)
                     /locus_tag="SOI88_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WPQ07343.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3705..3962)
                     /locus_tag="SOI88_29660"
     CDS             complement(3705..3962)
                     /locus_tag="SOI88_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WPQ07344.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3972..5657)
                     /locus_tag="SOI88_29665"
     CDS             complement(3972..5657)
                     /locus_tag="SOI88_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WPQ07345.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5762..7249)
                     /gene="ltrA"
                     /locus_tag="SOI88_29670"
     CDS             complement(5762..7249)
                     /gene="ltrA"
                     /locus_tag="SOI88_29670"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003964 - RNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WPQ07346.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7894..8139
                     /locus_tag="SOI88_29675"
     CDS             7894..8139
                     /locus_tag="SOI88_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07333.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8413..8784
                     /gene="tolA"
                     /locus_tag="SOI88_29680"
     CDS             8413..8784
                     /gene="tolA"
                     /locus_tag="SOI88_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WPQ07334.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8781..9146
                     /locus_tag="SOI88_29685"
     CDS             8781..9146
                     /locus_tag="SOI88_29685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WPQ07335.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9321..9599
                     /locus_tag="SOI88_29690"
     CDS             9321..9599
                     /locus_tag="SOI88_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WPQ07336.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9587..9901
                     /locus_tag="SOI88_29695"
     CDS             9587..9901
                     /locus_tag="SOI88_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WPQ07337.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10065..10493
                     /locus_tag="SOI88_29700"
     CDS             10065..10493
                     /locus_tag="SOI88_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07338.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10519..10698
                     /locus_tag="SOI88_29705"
     CDS             10519..10698
                     /locus_tag="SOI88_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WPQ07339.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10725..11255)
                     /locus_tag="SOI88_29710"
     CDS             complement(10725..11255)
                     /locus_tag="SOI88_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPQ07340.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10657
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2788..3368
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.113"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11266..11972)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.068"
                     /coverage="97.0"
                     /evalue="1.42e-136"
ORIGIN
        1 gttgtactgc tccagtgtca tcagtaaagc cccgcaaaat catcatccgc ttttggtccg
       61 ccgccgtcgg ccggaggcag gtactccgcc agcgggtccg ttttcgccag caccgggaag
      121 gttttcagcg aaatttcccg gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc
      181 acgcccagcc cggtcatcac cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca
      241 aacagcggcc gcgcctcctt ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc
      301 gcctcccggc tggcccggcg gcgctccacg tccacccgga tctccccggt ctggcccgcc
      361 gcccactgcg cggactcctg gctggccgcg cgatagaacc agcggatcgg cgtgttatcc
      421 agtaccgtgg ttttcacaaa atcagcgggc agatcctgcc cgcactgacc gaagtcgccc
      481 agcgactggt gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc
      541 agcacgtact tcgacagcag atatttaatc tcatcgagca tgatgctcgc gtgcggccac
      601 ctccggaact catcccgcgc aatgatgatt tgcgcgcagc gggcaaacag catcttctgc
      661 acccggatca ccgcctcatc gtccatcgag ccgatgacgt acaggcagcc gccgccgttg
      721 attatccccg ccacatcgat accgctgtcc gtctgcagga cgctcaggtc ggccacgttc
      781 tccagcttgg tgatcagccc cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc
      841 gccgccagca cctgctgaat gtttgccccc tgcgggaact gctccgcaat cagcttcgcc
      901 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcact caggttaaac
      961 ccggcaacca gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc
     1021 gccggtttcc ccggccgcag atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg
     1081 tgcggcgccc acgcgtcccc tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt
     1141 acgcagcgaa tgagcgcgtt ggtggccatc gcgctcttgc cgctcccggg caggccgagg
     1201 atctgaatat tggtcttgcg ccaggtatca cgagggacca gcaccggctg gccctgctca
     1261 tccttaccca gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc
     1321 gcgttaaccg gtaccggctc cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc
     1381 cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc
     1441 gcaaaatgac catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca
     1501 agccctccgg cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct
     1561 gcgttcgcat agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg
     1621 acaaccagcc cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc
     1681 cagacatgtc gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa
     1741 aatggcacag tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta
     1801 tggctcagcc ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg
     1861 ccgataacca gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac
     1921 gggtaaatct gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta
     1981 aacatgcaat ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg
     2041 cacaagccgc ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt
     2101 tcagctttca ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg
     2161 aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt
     2221 tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc
     2281 aggagcagct ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca
     2341 ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga
     2401 gccagaaaga ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt
     2461 ttcccctgaa gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa
     2521 aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc
     2581 ctgataattg atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc
     2641 gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc
     2701 tacgctccgg gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat
     2761 tccgtggata agcaggggac taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt
     2821 ttttccatag gctccgccct cctgccagag ttcacataaa cagacgcttt ttcggtgcat
     2881 ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact
     2941 taaagatccc caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg
     3001 ccgtttaccg gatacctgtc cgccgttctc ccttgcggga agtgtggcgc tttctcatag
     3061 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
     3121 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcatc ttgagtccaa
     3181 cccgggaaag cacgcaaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga
     3241 ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact
     3301 ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc
     3361 ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca
     3421 aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc
     3481 gcgatccccg tcagttcaga ggaggacgat ggtgccaccg ttccgccctg aacatcccgg
     3541 atataatttg cctgacatgc ggccagaaga aagccagcaa caatcaatat aaaaagaaaa
     3601 atagtcttta cctttttcat atactttccc tgtatttaaa gactccggca ttcatccctg
     3661 aacaccggaa gtcagtattt caaatcatcc ttgataattt tttgttacca gttaccgtcc
     3721 cgataatcaa aagctatata acaattatcc acatcaggtt ttatttgatt tttaaaaaat
     3781 ggctgtagta aaggaaccca ttctttcttc aaatcaaact caccattatt aatattatcc
     3841 tttaaaggca ttcccaagct ttcaatgaca gaaccatcgt caccaaagtc tttagaatac
     3901 tcttcccctt taaactcttc agttttctta tcaaaccatg tcaaacgtaa tttaagcccc
     3961 atatttacct ctcaaagata ttttttaatg ttccgttttg gatctggccc tttaacctgc
     4021 ttacccgttt tgggatcaaa tgcgcctagg tgttcgccat cactggcacg atacccttca
     4081 agctcaccgt gctgggaatc ccattcataa attttacgtt ttttatcgcc ataccatcgg
     4141 gcccgcttac caccaccacc ttgtttaggt gttttaggat cacctcgctt taactctcca
     4201 agtcccttta tgtcttctgt tttaggtact ggatgataat catggccgta gtctttagtc
     4261 cccttgcgag gcttgttctt ttcctcattt aacttatttt ctgcatcctt cgcttttttc
     4321 tccttttgtt tccggctctc catcgccccg gtaagtgcag ccttatctct ggataaggat
     4381 tcattcattg cgtttaaaga tgcctgagcc gcttttaatt tctcattctg attctgaata
     4441 tccttatcaa gatagcgaac ctgatcctgc cacccggagc catattcatg aggcgggtta
     4501 taagtcacaa aatcatcttt atttgcctct gcctctttta cctttttatc ggcttcctga
     4561 acagcagccc ttgcaccggg gagcgccttc tctgtcgatg caatgcggct gtttaaggtc
     4621 gcaatatttt tatcttcggc atccagctca gctttcgctt tatcatagtc tcttttcagc
     4681 ccctcttccg gatgagcggc gtcccatgcc tgctggcgac gcttttcttc ctcctgacgc
     4741 tgttttacct gtgccggggt aagaacatct gtcaccgaca cataaaccgg cttctgtccg
     4801 gtctctttcg ggaaacgaat aacggcctca cgggagttac caccggctgt aaaaccagcc
     4861 ggacgtgaat cacctttttc agcaacaatg ccttttggcg gggctttcgc tgtcggaaca
     4921 cctttcggta cgctcacctg cagagacggg agacccggaa tcgacacact gaataccccc
     4981 ggacgttttg tcggtttcgc atccaccaca gggacactca ttggccgacc tgcgacaacc
     5041 gcaatatgct gccgctcatc cttcaccaca tccacaaccc gttgtctgac acgaaccgtc
     5101 gcctggtcca gtggtaaagt acttgccgga gtctccgtca ccgcatcggc cggtaatgac
     5161 gtcataattt ttgacatcat tttcggatca tcttttgcaa tctcagaagg cagcacaccg
     5221 tagatcgcaa tcccccacag accaaactta aacggcccct tcagggcagc cagcacatca
     5281 gcaacggcgg ccgacaacgc atcgccggaa actgataaag ccagtccttc agcccccgga
     5341 gtagccaggg caggcagacc gaaggccatc gcagtggccg aagactgtcc gccaccagag
     5401 ctaccgctat gattgccgct gttaccacca ttaccaccgg gtttactccc gccgttacca
     5461 aactcccccg gattatagtt atgaatatct ccattcggcg tatgcgtcgt accccagccc
     5521 ggcccgctgt ttggatccgt accaccacct gatgaagggc cacctttacc agacgtaccg
     5581 ttcacattcc cactggcctg accaccatta tgtcccagac ctgaattacc cggacctcga
     5641 ccgtctccac cactcatacc gttttcctct ttgacattta aaacaataaa ttaaagccaa
     5701 catgtcgcgg tagggatacc cgttaccgga taccccccgc acagatcccg gcgtgcgcga
     5761 tttacgcacc gggctcctgc ctcgggtgtc tggcggtgaa ccgctccaca ggccatggat
     5821 gaagaacccg aacccttggt agccatgcgg ctgccagttt gtttgctttc gtccaggtcg
     5881 tatcatcctt ctggctcctg cgcctgagcg cccggcgcca gaggtttgtt acgtgtgtcc
     5941 tgaacttctg catggtgggg aagttgcccg gtaccgagtg atagttcagg tatccctgaa
     6001 ccactctcct gagccatttt ccctgttcgg ggattgagta atgccagcgc cttcgcagac
     6061 cgtctttgat ggctttcaga gttgccgtca tccgatcccg gcgggtcttt cgtatcagca
     6121 tgaacctgcc gttgcgatct ttcccgctga tgtgcgtgaa cccgaggaag ttgaacgttt
     6181 ctggtttgcc ttttcccctg atggcacggt tttcggcagc gaagcggccg aactccatca
     6241 gacgggtttt ctccgggtga accgtgagtc cgaactccct cagtctgcgc tgcatggcta
     6301 tacggaagcg ccgggcatcg tatcgtttgt cgaacccgat gacgatgtca tcggcgtatc
     6361 tgaccattac cacattgcct gtggcatagc gacgtcgcca ctgatgcgcc cacagatcga
     6421 agacgtagtg gaggtatatg tttgccagca gcggtgagat gaccgcaccc tgtggggtgc
     6481 cttcctccgt tgctcgccat tgaccctcct ccgacgtccc ggctgtgagc cacttacgta
     6541 tgagcctgat taccctccgg tcgccgatcc gatgctctgt gaacctgatc agccattcgt
     6601 ggctcaccct gtcgaagaac tgactgatgt cggcatccag tacccagttt acgttagtgc
     6661 gtaccagccc tgtggccagt gcgtccagtg catcgtgctg gcttcgcccg ggtctgaacc
     6721 cgtatgagaa ccccataaag tcgttttcat agactgcgtt caggattttc accagcgcat
     6781 actggacgat cttgtcctcc agcgaggcga tgccgagcgg gcgttgtttt ccatccgctt
     6841 ttgggatgta gtgacgcctg ccgggctgcg ccctgtagct gccctgatgt agcctccggt
     6901 gcagatctgt tatgttgttc ttcatgtttc cggcgtagtc catccacctg atgccatcca
     6961 ctccggcggc cgctttcctg ctcagggaga ggaatgcggc ttccagtgct tcgactgtca
     7021 gcaggtggaa caatgctgta aaccgttctt tcttccgctg cttcgcagct tcccgcacgc
     7081 gtgacagcct ctgtgacatg ctttcccggc tctgtgtccg gcgcatgtgt ggctgttccg
     7141 cgttcccctt ggccccgctc cttcgctcca ctgactccgc tcctttcggg ttgttcgcct
     7201 gcttcgccgc tactatgagc gagtccgact tctcctctcc gtacatcacc ggctatgact
     7261 cctcgtcttc ccggtgcggg ccatctccga cactggcaga tggtcagagg ggagatctcc
     7321 cggttcccgc gtagagatcg tattgacatg ccagggtctc agaccccgcc gggtccatgt
     7381 ggcactcgca gtgtcgcacc ctatgatgtt gccttccgtt aacagtacaa cgtcggcacc
     7441 cggtaattta atatacattt cgtggctcaa tggctggcct gtcaacaccc ctgtcaacgc
     7501 ttcgccccat acctcgcggt atgcaacgca tgactcgggg accttgtgga ttgctggtcc
     7561 ttcaatggtc ggggactttc accccttgat ctctaaccgg tctcccggcg cacactgtac
     7621 atatacacag tataattatt accttactcc tcccgttttc aggtgtcaat atatgagatt
     7681 agttctcatc ttcaggcaca aaaaagcgcc cggagccacc tgtaagcaga tcgcgtcttt
     7741 tcccgaaaat ttctcatggc aacaaatttt attatttaag acattcatca cacttacatt
     7801 tcagatggta tagacacatc tgaaagctaa ctggcatgag gagaggacct gcggttaaac
     7861 tgttcgggaa acctgagacg cccggaggcc actatgacgc acctgaaact gactcccgct
     7921 gaagaaaagc gcctggcgga cgcctttgtc gctcaggcac tggccatggg cctgaaaggg
     7981 aacaaaacgc tgccaaaggc ggtaaaacaa caggtgcggg aaagggtgct tcgtgccatt
     8041 caggacgaac gtgaagccag gcgggcgcag gaaatgcgtc gggagaaata cgaggctacg
     8101 gaaaccacct tcacctggca gccacagcgc cgccggtaag gggtatttac cataacgtcg
     8161 cattgcccgc accgctcaga cggtctgagg gcgtttccgg cggctacggg ataaaacagg
     8221 caacaaacca cccggaaaac cgccggaaac gcgcccggta aattgtaacc cgctgcatgc
     8281 ctatgcacgc aactgcatgg cgggccgttt ggtgtaaaaa cgatacgata agccgtgttt
     8341 atgtaaatga acgtggccgt aaagagtgac aaatatacat gtaacatttt ttactgacag
     8401 gtcatcaacc cgatgcgttt aatacccttt atcctgctgg cattgtgcag ccccttactc
     8461 ctgacggcct gtaagcattc caccacccag ccgccgaaaa tttatactct ggataagtca
     8521 tctaaaatca gtagctacgc tgcggccatc aagaccgctg ttgaaatgca gatgtttgaa
     8581 ccagaggcct atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg tgatggcact
     8641 ctgaacagcg ccattgctga aggaggagat ccgaaactct gtaaagcagc aatatcagcc
     8701 gttacccacg ccaaaattcc gccggcaccg gatgaaaata cctggcaaat attcaaaaac
     8761 gcccctctgg atttcagacc atgaaagctt ttactctgac agtgctggcg gccatgttcc
     8821 tcactccggt acatgcgata ccactaaaac ccgtaaccac actgcagcca acctacccgg
     8881 tacaggccgc agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat attctggacg
     8941 atggacgagt ggacaacgtg tcgcttaccg gctgtcacct tttttttcgg gaaacgctga
     9001 cggcaatgag gcactggcta tatcccgcag ggcacccggc aatgcgacag gttataaatt
     9061 ttgaattcag tctgaaaaat gacggcttac aggatccgcg accagtcacc acccaaaagc
     9121 tcaatgatga tggttgccgc cagtaacagg tcctttaacc cgcaggctgc aatagtcctg
     9181 gatgatgagt gagcctgtag cagccctgat tagtggaagc gaagctgaaa aatgggcagc
     9241 aaaagccgta tttctggcaa atagcgccgc cacggattaa agtagtcatg tgtaatacat
     9301 atgactacaa aggggctttt atgtcatcag caacatccat caggctcgat gaagaactta
     9361 aggaccggct gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg
     9421 aggcaatcac cgaatacatc gaccgggaag agaagcgcag ccagtacctg cgggacggac
     9481 aggccgcatg gcagcactat caggaaacgg gtctgcacct tactgcggaa gaagcggaag
     9541 cctggataag tacctgggga actgagaacg agcaggatgc tccgccatgc cacaggtaac
     9601 gatttccgcg ctggcacagc gcgatttaca acgccttcag gactttctca aaaccaaaaa
     9661 tcggctggca gccagaaaag gaggtgaggt gatcgtccgg gctatccagc aactgaaaac
     9721 attgccagac attggccgcc cggtgccgtt tctgccgctg gaatataagg aactggtgat
     9781 cgggtttggc gacagtggct atgtgatgct ctaccgccac gaccgggaaa tggaccagat
     9841 tgtgatcgtc accgtcaggc atcagaaaga atccgggtat ccgggggcag acagcctcta
     9901 ataaccgatg acgggggcgc aattcattgc gtggccgtcg ccggtttgac aagcgcagcg
     9961 cgtcagaaaa ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg
    10021 agaaattaca cacctttcac acccgataaa atcaggggta tacagtggcg aaaacattgc
    10081 tcaacctttc acaggcagca caagccgcgg gcattaccag aagaacgcta tacaatcatg
    10141 taaaacaggg aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt
    10201 cagaactgat ccgtgtatac ggaaatgtga atatccctga gaaacaaatt cccggcattt
    10261 cacaccgtga aaatacacaa aaaaatttcc cacaggaaca gcttctggca atgcaaaagg
    10321 aactggccga tctccggcag gctgtcaccc tgatgctgga agataaaaca tcccgggaag
    10381 aagaacgccg ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg gacagattga
    10441 caacagaact gacacagaag aaaaaacgct tctggtccgg ctggtttagc tgagaactta
    10501 ccgaagggaa tggagaacat gacgaagcag gaaaaaactg ccctcaatat ggctaaattc
    10561 attaaggatc agtcgctgct gttgctggaa aagctgaacg agctggacat ggataccccg
    10621 gccgacatgt gcgaacggct tcacgaggat gcggaaacac tctggcaggt gatgcgggat
    10681 aagctcgggg aagaataaca gcaccggcca tgccggtgct ggcgttattt tttgccgaac
    10741 ggccaccacc ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac gttaaaatag
    10801 cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc gctttccccg
    10861 tgccgctcgc tgaccacctc aatcatcgcg ttaatctgct ccggtgagca cttcccggca
    10921 tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca cctttcgata gaggtaatca
    10981 cacagcttca ttcctgtggg tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag
    11041 tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga tttcatcatc
    11101 gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg ccaggcgagc
    11161 accaccatgt cttccagatg ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct
    11221 tccacgtcct caataaatac catgtacagc gccataatcc ctcactccgt tgtcataaat
    11281 ttaaagcacg cgaacagcct ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc
    11341 tgtggattca cgatttcatc caccgccaga atcgcccgct gtacccggac aagcgtcggc
    11401 cggtcagggc agatgtacca gatttcatcg atgccgttcg cccggcggtt gaacagatac
    11461 ccggacacca cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc
    11521 accgttctgc cgtccagctc cgccagcgcg tccggcactt tcatcccttt cagggccata
    11581 cggtgcaggt accgccagcc ggtggccccc cggccctcca tggagaggcg cagggactgc
    11641 acggccagcc ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa cggaataacg
    11701 tccgtcagcg gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac
    11761 acgctgacct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc tttcgccacc
    11821 agactgtcca ggaagcggta aaccggctgt accgccgcaa tccccagccg tcgcgcaacc
    11881 agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg tgttttctcc
    11941 gcgttccgcg cctgccgctt cgcgtggctg ac
//
