LOCUS       CP137928               14639 bp    DNA     circular BCT 15-NOV-2023
DEFINITION  Acinetobacter baumannii strain 2023CK-00001 plasmid unnamed1,
            complete sequence.
ACCESSION   CP137928
VERSION     CP137928.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN32801359
KEYWORDS    .
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14639)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 14639)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/06/2023 11:17:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,894
            CDSs (total)                      :: 3,800
            Genes (coding)                    :: 3,674
            CDSs (with protein)               :: 3,674
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 37 of 126
            Pseudo Genes (incomplete)         :: 90 of 126
            Pseudo Genes (internal stop)      :: 12 of 126
            Pseudo Genes (multiple problems)  :: 13 of 126
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="2023CK-00001"
                     /isolation_source="Tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA"
                     /collection_date="2022-12-27"
     gene            1..951
                     /gene="repM"
                     /locus_tag="PJT01_19385"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="PJT01_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WPF18508.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLDLFSKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="PJT01_19390"
     CDS             944..1519
                     /locus_tag="PJT01_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WPF18509.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQISQQIEPSKSDNDESTKKLIIHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQDNDYKKNNELNIVENVAVPQQETTAYHSQTLQH
                     ATLQNVAVPQPKKRGLFGRVLNAVFNEE"
                     /name="plasmid replication DNA-binding protein"
     gene            1541..1684
                     /locus_tag="PJT01_19395"
     CDS             1541..1684
                     /locus_tag="PJT01_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPF18510.1"
                     /translation="MKKLKEILQGIAFLFVGIPIMIMASIIDQRAFKKEMDERQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1991..2266
                     /locus_tag="PJT01_19400"
     CDS             1991..2266
                     /locus_tag="PJT01_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WPF18511.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALEDNVECGAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRKEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            2288..3397
                     /locus_tag="PJT01_19405"
     CDS             2288..3397
                     /locus_tag="PJT01_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004024 - alcohol dehydrogenase activity, zinc-dependent
                     [Evidence IEA]; GO_function: GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase activity
                     [Evidence IEA]; GO_process: GO:0006113 - fermentation
                     [Evidence IEA]; GO_process: GO:0009404 - toxin metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WPF18512.1"
                     /translation="MKSRAAVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAF
                     TLSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSN
                     LCISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPD
                     KFALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGW
                     GQSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPF
                     VTHTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            3450..3848
                     /locus_tag="PJT01_19410"
     CDS             3450..3848
                     /locus_tag="PJT01_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WPF18513.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            4322..5155
                     /gene="fghA"
                     /locus_tag="PJT01_19415"
     CDS             4322..5155
                     /gene="fghA"
                     /locus_tag="PJT01_19415"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0018738 - S-formylglutathione hydrolase activity
                     [Evidence IEA]; GO_process: GO:0046292 - formaldehyde
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WPF18514.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            5666..5953
                     /locus_tag="PJT01_19420"
     CDS             5666..5953
                     /locus_tag="PJT01_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WPF18515.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5940..6254
                     /locus_tag="PJT01_19425"
     CDS             5940..6254
                     /locus_tag="PJT01_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WPF18516.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6382..8793
                     /gene="znuD2"
                     /locus_tag="PJT01_19430"
     CDS             6382..8793
                     /gene="znuD2"
                     /locus_tag="PJT01_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WPF18517.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            9098..9559
                     /locus_tag="PJT01_19435"
     CDS             9098..9559
                     /locus_tag="PJT01_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WPF18503.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(9880..10413)
                     /locus_tag="PJT01_19440"
     CDS             complement(9880..10413)
                     /locus_tag="PJT01_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPF18504.1"
                     /translation="MLYEAYYPSEGSDNHPIKIFYGFVQEGFEKGHQLTLVVFYPELTG
                     TSLFNLMDETKAGLANFLLQKHVYGVGASHIKVAFVCEHVNKRDFYTEIWGVDVFENYA
                     LEQLEQQPKSLLSGILGNNSKEVSIHSSYLNIGLTDSIKLIDEGHLDTEQTAYLLNKLK
                     EKTDYGNFNKIFGE"
                     /name="hypothetical protein"
     gene            complement(10481..12298)
                     /locus_tag="PJT01_19445"
     CDS             complement(10481..12298)
                     /locus_tag="PJT01_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WPF18505.1"
                     /translation="MLHNFFMSYYGYLINGLHLKPLFVGFANTLFALAIIVIFTMIVVG
                     IKKIYKAHDPVYEIGYNIVLLAVVVLAHTYLLSYITNLLPNNYVVVPEKNYFFSWAVII
                     GLYVYLYFTGKKSKPRVERIKKESAEGYGTLLSELKRGFENKTPLIAFTDQTLPKKQIK
                     EMEQKYLDWSFHRESLRVTNEDRGIVLGAPGSGKTTFLISQIVEWMKSGRSFVATDVKP
                     EIWAILKANNVFKIFGYTDWVFNPTDVDSDHYNIFSEVKDSAELNEILNIIIKDDTADS
                     VVFNDLARRILKAVLIELGDKASLVNAQNYINSMDSNDELLKALRQSEKSSVSNIAKEV
                     SRTAQNDRLLASVMTAVSKAFEFLDDDRIRVTTSDNTQGFYLKEVLMKPKQAVFLQFDQ
                     QYKSSTATLFGAMVAHTMRILQANQNREAVFIALDEIINCAPIPKFTDLLNTIRSANMP
                     TFLYLQSLEGLNRLYGANSDKMFMGSSNLKIVFRIGDIESAEECSRLVGQTETTYISET
                     AGTSQTSGTSSSSRASSSSSNRSQNTGTTKSIKLESIIEPAEFIKLPICTAVVMYNGSY
                     GTLEMPKYYECYNMPKRTNLKTIRDFKVA"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            complement(12299..12538)
                     /locus_tag="PJT01_19450"
     CDS             complement(12299..12538)
                     /locus_tag="PJT01_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPF18506.1"
                     /translation="MGIASEIRSSLPLCGRCINCKLLIWNDKAELKSTQNLIKFRSSEA
                     SFYYTVRCSWLKSPVSEPQFLDTCEGKQQQKGSD"
                     /name="hypothetical protein"
     gene            complement(12539..13432)
                     /locus_tag="PJT01_19455"
     CDS             complement(12539..13432)
                     /locus_tag="PJT01_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="WPF18507.1"
                     /translation="MTLEYQFDQLCNKFREGSHATQANRRAMLKLFTEQLAKNGYDVKT
                     MSPKDLKGRHVNKLLEQWRKDGVSTGTIKNRMVAVRWWAEKVGNSGAVKDNATYKIENR
                     VYVTNENKSISLKDLDLSKTDPNIAQSLRLQDAFGLRREESMKFQPEYALDGQKIENAK
                     YIRLKDSWTKGGRPRTIPITNEKQRQELKNAYAQAVKNGGSMIPKEKSYKSHKANFEAV
                     THALGVGQTHGLRHGYAQTRYRELMGFDCPAVGGSRSLSADEQVKDKEIRMLISEELGH
                     SRINITSVYLGSWSKN"
                     /name="integrase domain-containing protein"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.266"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa agacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa ccagactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ctatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga
      661 ttttctttta cctttaaaca gaaaaagaag gatagcccat caatagaaag agatccgaat
      721 accttagacc ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaacaaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaactg aaagctatcc tcaatttgct
      841 gttcgtattg ctgagatgct acaagactct gaaaaaatca aagaactagc cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacacag taaatggcta agttatcgct
      961 aagtgaagta tctaaaaaat ttcatattga taggtcaacc atttatagag ctgtacgtaa
     1021 tgggcgttta tcacgctcta gtgatggtca atttgacctt tcggaagtga ttagatgctt
     1081 tggggagcct gagcaaatat ctcaacaaat tgaaccatct aaatcagata atgatgaatc
     1141 tacaaaaaaa ctcattattc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 gcgtttaatg cagcaaatcg accgtatgca gacgcttatt gagttaaaaa ctgttgcacc
     1261 cgccacggca gcgccacaac aagatgctac ggcatgcgac accgatatgc cacagcatgc
     1321 gacaacacaa caagacaatg attataaaaa gaataatgaa ttaaatattg tagaaaatgt
     1381 ggctgtgcca cagcaagaaa ctacagcata ccacagccaa acgctacagc atgccacatt
     1441 acaaaatgtg gcagtgccac aacctaaaaa acgaggctta tttggccgag tactgaatgc
     1501 ggtattcaat gaagagtaag ctctccaagg agcattgttt atgaaaaaac tgaaagagat
     1561 cctgcaaggt attgcctttt tatttgttgg gattccgatc atgatcatgg ccagcatcat
     1621 agatcaacgt gccttcaaaa aagagatgga tgaacgccaa aaagaaaaag accaggcatc
     1681 ctgattattc aattgaccta atcaaagttg tggaacattg ctcacttatt aatcgtggaa
     1741 catgaaaaat ccacaaaaaa gcccaatcag gtgtgattgg gcttttttgt ggagtctaag
     1801 atgatgattt ataagaaata aaaatttgca tttcgtataa ggcgtattat gttaatttta
     1861 taaaaactta aaaatagata tagaattttt taattataag cttttatcaa ttattaattt
     1921 tataaaggct tcttgtcatt ctatatacta tagggtacta tactataata aaatttaagg
     1981 aattagcttt atgccaaatc aagtcgaaga taaaaaaaag attctgacgc gtgtcagacg
     2041 aatcaagggt caggctgaag cgattgaaaa agctttagaa gacaatgtgg aatgtggtgc
     2101 aattctgcaa caaatatgct ctgtgcgtgg tgctattaat ggtttaatga atgaaatgtt
     2161 ggaggttcat ttaaaggaca ctttagtatc aggtgaaacc acagaacaac aacgaaaaga
     2221 agaattggct gaaatcgcca agattctaaa atcatattta aaatagatct gtataaggaa
     2281 ttattccatg aagtcacgtg ctgctgttgc gtttggtcct ggtcaacctc ttcaaattgt
     2341 tgaggttgat gttgcaccac cgaagaaagg tgaagtatta atcaagatct cccatactgg
     2401 tgtgtgtcat acagacgcat ttactttatc tggcgatgat cctgaaggcg tttttccggc
     2461 tattctcggg catgaaggtg ctggtgttgt agttgaggtt ggtgaaggcg tgactagcgt
     2521 caaaccaggt gatcatgtta ttccacttta cactgctgaa tgtggcgagt gcgtattctg
     2581 taaatctgga aaatcgaact tgtgtatttc cgttcgcgaa actcaaggta gaggcgtaat
     2641 gccagatggt accactcgct tttcctacaa tggtcaaccg atttaccact atatgggctg
     2701 ctcaacgttc agtgaataca ctgtagtggc agaagtatca ttggctaaaa tcaatcctga
     2761 agccaatcac gaacatgtct gcttactggg ttgtggggta acaaccggta tcggtgcagt
     2821 acataacacg gctaaagtac aagaaggtga tagtgttgcg gtctttggtt taggtggtat
     2881 tggtttggca gtcgtacaag ccgcacgtca agctaaagca gggcgtatta ttgtggttga
     2941 catgaatcca gataaatttg cgctggcaaa agagtttggt gcaacggact tcttgaaccc
     3001 taaagactac gataaaccaa tccagcaagt cattgtcgaa atgactggct ggggtgtaga
     3061 tcattcattt gaatgtattg gcaacgtgaa tgtaatgcgt gcagcccttg aaagtgcaca
     3121 ccgtggctgg ggacaatccg taattattgg tgtagctggc gcaggtcagg aaatctctac
     3181 ccgtccattc caactggtta ctggtcgtaa atggctaggt tctgcctttg gtggcgtaaa
     3241 aggtcgtacg caacttccag gaatggttga agatgccatg aaaggtaaaa ttcaattaga
     3301 accatttgtg acacatacga tgggcttgga tcagatcaat gaagcctttg atctaatgca
     3361 tgaaggtaag tcaattcgca ctgtcattca ctattgatcc acggatcaat acctaatttt
     3421 tcaaaaccaa ccatcaagga aacactttaa tggctactcc agtaatttcc ggtaacgata
     3481 ttttctccca tgtcttcctc ggcgcagtcg accttaagaa gtcggttgta ttctacgatg
     3541 ccgctctagg tgccctcggc attaaaaacc tagggccatt cggtaacgat tgggttctgt
     3601 acggtcgcga caaacctgct ttcatgatca cccgcccaag caatggcgca gaacccacca
     3661 gtaatggtgt gacggtaggc ttcgcagctg ccacccctgc agaggtaaat gcattccacg
     3721 cagccggcct cgccgctggt ggcaccgatg aaggtcagcc tggcccacgt ggccacctgc
     3781 cgggtgctta cgcggcctac ctgcgtgatc cggctggtaa taaggtctgc gcctacacct
     3841 ttgtctaaaa cctaggagaa gtgaggtcat tgtgtaagga ctggttccag ccaaaaacaa
     3901 tctcatcttc cactgcaacc gtgtggcatg cccgctcatg ccacacggtt tacggtactc
     3961 tgagcctcta tctgctgatt agagctcaga aaggctgtta cctcgccttc atagccaaag
     4021 acggtcaatg agcacgatcg gactgaaagt tcggtgcgtg atgcaagaaa cacagaatcc
     4081 aaaatgagca tcgcggtagt ggctccctga attacacact ttattgtttg tacattgaag
     4141 tcgacatccg accttcgtgg tgtggtggcg tctgggtttg tagcagcgat cttcatgcca
     4201 gcgacaacgc tgaaaacctg ctgagtggtg cggtgaagca tttttaacta atcctggatg
     4261 gaacgaaaat tcgtcttgat tggcgcctga gcaccacctc gttgaaataa ggagaaaatc
     4321 cgtggaacgc attgaacatc acgccagctt cgagggttgg caggacgttt ttcagcatga
     4381 gtcaacagtg cttggctgca tgatgaggtt tggaatctat cttccgcctc aggctgagca
     4441 tgaaaagctc ccagttctgt actggctctc aggtctgacc tgtaccgaac agaacttcat
     4501 caccaaggct gcggtgcagc ggtatgccgc ggagcacgga atcattgtcg tagccccaga
     4561 taccagcccg cgcggagaaa gcgttgcaga cgatgctgcc tatgatctag gtcaaggtgc
     4621 gggtttctat gtagacgcca cagagctacc ttggagcgat cactatcaga tgtattccta
     4681 tgtggtcgac gaattaccag cgctgatcga agcgaacttc cctgcgtcgt caagacgcag
     4741 catcagtggg cactccatgg gcggtcatgg cgctctggtt attgcgcttc gcaaccccgg
     4801 tcgctatgca agtgtctcgg cgttctcgcc gatcgtggcg cctacgcaag ttccatgggg
     4861 gcagaaggct ctggcggctt acttgggcga aaagaatctg gaagcctgga agcaatatga
     4921 cacggtcgag ctgattcgcg tggcgcaaga acgcttaccg ctgcttgtcg atcaagggct
     4981 gagcgatgag tttctcgagc accagcttcg accgcaactg ttgcgtaccg cgtgtgaaca
     5041 ggtagaccac ccgctaacgc tcaatctgcg gcctggtcat gaccatagct actactttat
     5101 ttcgacctac ttgagcgaac acatggccca ccatgcggct gcattgaatc gctgaacatt
     5161 gggagaccct gacttagaaa tgtctgtata ggacatttct atttggtttt aaggggcttt
     5221 gttgcataaa tacataacca actgattttg ttcatttaat ttttaggttg aaattcatac
     5281 aaacctattt aaatcagaag cttacaaaat ctttatgcaa caaagcccat atcagataaa
     5341 aaagtagagg agctaatact aagccgtgat ttcttggaaa agctgaaaga gctagttaat
     5401 cgaacagaaa taccatgtag ttaactatcc atttaacata atggatatta tacgaagtac
     5461 atttgtatat taatttaaat atcaactact taataaaaat ataaaaatcc aaaaatgccc
     5521 aaaaagccga ctttgaaaca agtcggcttt tttatgatcg attttgatag tttttgccaa
     5581 taaatctgac taaaaattac gcttctcaac ttgagtgata caatttcgtg atacattatt
     5641 gtatatacaa atttgaatgt tttttatgga acagtatttt gaatgggatg aggctaagaa
     5701 tcgaaagaat cagaaaaagc atgatatctc tttcgaaact gcaagccttg tttttgaaga
     5761 tccattacgg atctcaatcc aagacagata tactaatggt gaagaacgtt ggcaaaccat
     5821 tggacgagtt aaaggcgtac taatgttatt ggtggctcac accatttttg atgaagatga
     5881 ctgtgaaatc atccgaatca ttagtgcaag gcaagtcact aaagcggagc gaaacaaata
     5941 tgagcatggt tagatactca cacaaagagc tgaatgagaa atttggtgag aagcaagatg
     6001 ctgaaattca acgcttgctt gctaaaggca cggttcctga tgatcagcta gatctatcag
     6061 atattcctga aattacagac tggagcaatg ctgtacgcca aaatcaattt taccgcccag
     6121 taaagcaaca aacttcaatt cggttagatg ctgatgtact tgcttggttc aaagcacaag
     6181 gcaaaggtta tcaaacacgt atgaatgaaa ttttacgaga tgctatgcta aaagaattaa
     6241 aaaatcatca ataagaatgg gagcttaggc tcccattttt gacttcattt ttagggggga
     6301 ttaccattca aaaatttaaa tagtagattt tataaatttt taagtgttat attataatat
     6361 aacattaaat ttggttgttt tatgctaaat aaaagtaaac tatttttagc tttaattact
     6421 ttaggggcct cgaaaatttt attggcagct gaagggcctg ttactacctt aaatacaata
     6481 gtattaactg cacaatctga tgagttaggg tctgagctgt taggcaaaag tctaaatgtt
     6541 agtaatcagt ttattgatac tagtaaactt aaacagcgtt cgacaactct aggggatgcc
     6601 ttgggtacgg aattaggaat tcattctaat caatatggag gtggagcatc tactcctatt
     6661 attcgtgggc aggaaggaaa acgtattaaa gttttacaga ataatgctga cgtgttggat
     6721 atgtctaata tgtctccaga tcacgctgtc acagttgaac caagtttagc taagtctatt
     6781 gaaattatac gtggtgcaag tacccttttg tatagttcaa atagtgcggc aggggtagtc
     6841 aatgtcattg actataaaat tccaacccaa atgcctcaag acggattaga gggtaatact
     6901 acattacgtt ttaatactgg cagtaatgag aagttgacca cagcaggtgt aacagttggc
     6961 ttaagcccac atgtagcttt acgggcagaa gggttatatc gtaatgctgg taactataaa
     7021 actcctcatt accagtctag ctcttataat tctttagaag atcttgaaaa ccaaaacagt
     7081 atttataaaa atttaaaata tttaccagaa agctgggcag agtcgagagt aggtacgctt
     7141 ggattatcct ggatagacga taatacttat ttaggagttt cttatacaca tcgtcatgat
     7201 gaatatggtt taccagccca tagccattta tatgaaggtt gtggtgcctc tgcaatcggt
     7261 atcgattcac gaattagtgg attaaaaaat tatcttttat attatcctca attgatggat
     7321 gagcaagata ttaattacat aaatccacgt cctgattgtc atcagcataa tcatattcat
     7381 gaaactaatt tctctcataa tgctccttat attgatttaa atactcgacg ttatgatgtg
     7441 cgtggagaat tcactcaacc atttaccggt attgacaaaa ttcgtacctc attgagttat
     7501 atagattatt ttcacaatga actcgaggga gataagatta ctaacttttt taaaaataca
     7561 ggaaaagtag gccgcataga gttatcacat cagccattag gggaattaac cggtatcttg
     7621 gggctacaat atttggaaca ggataatagt gctttatcac cagtccattc tcaagaaggc
     7681 cataccacct atctagataa tcagcagcta ttaaatcgaa atgtcactaa aaactttagt
     7741 gtatttggcc tagaaaaata taactggaac gactttactt tcgaactagg tgctcgtata
     7801 gaaaagcaga aagttagtat ggattatgat atagaaaaaa ttaaagattc aatgaaacct
     7861 tggcctaata aatataacag tccttatgta gaaaaaaata ataaaatcag ggctcaaaat
     7921 ttaaaatcta ttttagaagc tgttcaacct aacaaagaaa cagctttttc ttatgcaggt
     7981 acagtccact ggagatttgc tccaaattat atcttatcat taacaggtac acatcaagaa
     8041 agattaccta atgcacaaga aatgtatact catggtatgc atctcgctac taattctttt
     8101 gaaataggta accgctttct aagaaaagag aaatccaata atttagaaat cagtctggcc
     8161 tataaggatg atttacttga ttatcaaatc agtacttact attatgattt tgataactat
     8221 atatatttac agacactcaa tgaagtttta ggaactacaa aagttagaga tcagcataca
     8281 ctacgtatta atcattatag ccagtctgct gctaattttt atggattaga gggcaacata
     8341 gggtatcaat ttaattctgt ttatcacggc tctctttttg gagattatgt gaaaggccgt
     8401 ttaacaaatt tacctgatgc tgtaattgca tacgatatct ggaatagaga accgacttta
     8461 gctccccaaa aggatcgtta cactcctaga ttacctcctg ctcgtttggg tacacgacta
     8521 aaagctgatt ttgatgaatc gcttaaaggt gaaatagaat attatcgagt ctttaaacaa
     8581 gataatattt ctaaatttga gcaggttaca tcaggttata atatgcttaa tatgacttta
     8641 gcatataaaa ataagctatc tcacacagaa tatgatttat tctttaaagc gaataattta
     8701 cttgatcaga aagtctatgc ccatgaaaca tttttgccat atattcccca aattggaaga
     8761 aattttagtt taggcttaaa tcttaatttc tagataagat actgtaatct tctaagaaaa
     8821 tttcttagaa gatttttctt ataaaatgat tagattaata attaaggtaa gcgtccgctg
     8881 aatcccatac ctatttttta attcgattta ggtttccagc agtgtggcag taactcttca
     8941 atctgggtca ctttatgtgt cggcagcctt ttcagcactg aatagccacc gattttgtag
     9001 acacctttta gctagataaa aagcaatttt tttgatcttt aaagaatgag gatataagct
     9061 gtttaaaact taattctaat tggttaatag gtgaaaaatg aaattagcag aagctctctt
     9121 acttcgaagt gatcaacaaa aaaaaattat ttcactaaaa caacgtatca atgcaaatgt
     9181 attggtacaa gatggtgatc aaccatctga agatccaaat gaactactaa aacaagtatt
     9241 ttctttaatt caagaatttc aaaaattaag ttatgcaatt catgaaacta atgctttaac
     9301 aaaattaaat gatggccgtt ctctacttgc tttattgact ttgcgtgatg aatttgtaga
     9361 acagcacaaa accctaactg cagcaataag taatacatct cgtgaatctg atcgttatag
     9421 tacgcgggaa ataaagtggc ataaagttat tccagtttca tcattacaaa aacaagctga
     9481 tgatattagt cttaaattac gtgatttaaa cgtgttaatt cagtcgacta attggaaaat
     9541 tgatttgatt gaagcctaat agtcctttag atataatcaa gttgaacata ctgggcgagt
     9601 acaagatccc ttacttccac aacataggat tgtaaataaa gtgtagtggc ttgcaaagca
     9661 agtggataag cttaaaattt taagtacaaa tcaatccatc acttggcata actcaaatct
     9721 ttgtgcaaac aaagttacac gttacttata actaccatgt actgacagta tgttcacctc
     9781 aattactaaa aataaagaaa tgagcaagca tcagctcaac tacatttcta tttttaattc
     9841 agttcggcat ttaacataaa atctcttata cgaaatcttt tactcaccaa aaattttatt
     9901 aaaatttccg taatcagttt tttctttcag tttatttaaa agataagccg tttgctccgt
     9961 atctaaatgc ccttcatcaa tcaatttgat tgaatcagtt aatccgatgt tcaaataact
    10021 tgaatgaata ctgacttcct tagagttatt tcccaaaatg ccgctgagta aacttttagg
    10081 ttgttgttcg agttgctcta aagcgtagtt ttcaaaaacg tctacgcccc agatttcagt
    10141 atagaagtct cttttattga catgctcaca tacaaaagcc actttaatgt gtgaagctcc
    10201 aacaccataa acatgtttct gtagtaaaaa atttgctaga ccagccttcg tctcatccat
    10261 caaattaaac aaagatgtac ctgttaattc aggataaaaa accaccaaag ttaattgatg
    10321 tcccttttca aacccctctt gcacgaagcc ataaaagatt tttattggat gattgtcgct
    10381 accctcactt gggtaatagg cttcatataa cactttttct agtactccta tattttcgtg
    10441 aaccgtacta aggtttttcg ttattaaaaa gttttgagtt ttaagcgact ttaaagtccc
    10501 taattgtttt taaattagtg cgtttgggca tgttgtagca ctcatagtat ttcggcattt
    10561 ccaatgttcc gtaagagccg ttatacatca caacagccgt acagatcggc aacttaataa
    10621 attcggcagg ctcaataata gattccaatt taatggactt ggttgtacct gtattttgcg
    10681 acctattgga agatgaactg cttgcccgac tcgatgaact tgtaccgctc gtctggcttg
    10741 tacctgccgt ttcagaaata tatgtcgttt cagtttgacc gactaatcga ctgcattcct
    10801 cagccgactc aatgtcccca attctaaaaa caatttttaa attgctagac cccatgaaca
    10861 tcttgtcgct atttgctcca tacaagcgat ttaagccctc taaactttgc agatagagga
    10921 atgtaggcat atttgcagaa cgtatcgtat tgagcaaatc agtgaatttt gggatagggg
    10981 cacaattaat aatttcatct aaggcaatga ataccgcctc acgattttga tttgcctgca
    11041 aaatacgcat ggtatgcgcc accatcgccc caaataatgt agctgttgaa ctcttatact
    11101 gttggtcaaa ctgcagaaat acggcttgtt ttggcttcat taatacttct tttaaataga
    11161 acccttgggt attgtctgat gtagttactc gaatacgatc atcatcaaga aactcaaatg
    11221 cttttgaaac agccgtcatc acagatgcaa gcaagcgatc attttgggct gttcgagaaa
    11281 cttctttagc aatatttgaa acactggatt tttcagactg tcttaatgcc tttaacagct
    11341 catcattact atccatactg ttaatataat tttgagcatt caccagtgat gccttatcac
    11401 caagttcgat gagaaccgcc ttaagaatac gtctagcaag gtcattaaaa accactgaat
    11461 cggctgtatc atctttgatg ataatgttta aaatctcatt gagttctgct gaatccttta
    11521 cttctgaaaa aatattgtag tggtcactat ccacatccgt tggattaaaa acccaatcgg
    11581 tataaccgaa aattttaaag acattgtttg ctttgagaat cgcccaaatt tctggcttta
    11641 cgtctgttgc aacgaagcta cgacccgact tcatccactc cacgatttga gagattaaaa
    11701 acgttgtttt acctgaacca ggtgcgccta acacaatgcc ccgatcttca tttgtcaccc
    11761 ttagactttc acgatggaac gaccaatcaa gatatttttg ttccatctct ttaatttgct
    11821 ttttgggtaa agtctgatcc gtaaaggcaa ttaatggcgt tttattttca aatccacgct
    11881 ttaattcgct taacaatgtc ccatagcctt cggcagactc tttttttatt cgttcaacac
    11941 ggggttttga cttcttccca gtaaaataca gataaacata taacccaatg attacagccc
    12001 atgaaaagaa gtaatttttt tctggtacaa caacataatt atttggaagt aagttggtga
    12061 tataactcaa tagataggta tgcgctaaaa cgactacagc aagcagaaca atgttatagc
    12121 cgatctcata tactggatca tgtgctttat aaattttctt tataccgaca acaatcatcg
    12181 taaaaattac aataattgct aaagcaaaca gcgtatttgc aaaaccaaca aacagcggtt
    12241 taaggtgcaa tccatttatt aaatagccgt aatagctcat aaaaaaatta tgtagcattt
    12301 agtcactccc tttctgctgc tgtttacctt cacaggtatc tagaaattga ggttcagata
    12361 caggactttt gagccatgaa caacgaacgg tataataaaa agatgcttct gagcttctaa
    12421 atttaattaa attttgagta gattttagtt ccgctttatc attccaaatc aatagcttgc
    12481 aattaataca acgaccacac aacggcaaac ttgatcttat ttctgatgca atacccatct
    12541 aatttttact ccatgaaccc aagtacacgg acgtaatatt gattcgacta tgtcccaatt
    12601 cttcactaat taacatacgg atttctttat cttttacttg ctcgtcagcc gacaatgaac
    12661 gagaaccgcc tacagcaggg caatcgaaac ccattaattc acggtatctg gtctgagcgt
    12721 agccatgtcg taaaccatgc gtttgtccca cacctagcgc atgcgtgact gcttcaaaat
    12781 tggctttgtg ggacttatag cttttttctt tcggaatcat tgaaccgcca tttttcacag
    12841 cttgagcgta ggcatttttg agttcttgac gttgtttttc gttggttatt ggaatagttc
    12901 gaggtcttcc gcctttcgtc caactgtctt ttaaacggat atatttagca ttttcaattt
    12961 tctgaccatc taaagcatat tcaggttgga acttcatgct ttcttcacgt ctaagcccga
    13021 aagcatcttg taaacgtaag ctttgcgcta tgtttggatc agttttagac aaatctaaat
    13081 cttttaaaga aatagattta ttttcattgg tcacatagac acgattctct attttataag
    13141 ttgcattgtc tttgacagca ccactattac caactttttc agcccaccac cttacagcca
    13201 ccatccgatt ttttattgtt ccagtggaca caccatcttt acgccattgc tcaagcaatt
    13261 tatttacatg ccgacctttt aaatctttag gcgacatggt tttaacatca taaccattct
    13321 tggcaagctg ttcagtaaac agtttcagca tagctcgcct atttgcttgt gtggcgtgtg
    13381 aaccttcacg gaacttattg cagagctgat caaattgata ctctaaggtc atgtgtcctc
    13441 ttttattcga gatgctttat tgatgagtac agcacagctt tatgctgggc tacggaatca
    13501 atgaagcatc atttttttaa tttataacta tttgagatcc gatgtactgg ttagtgttaa
    13561 gagccgaatt aacgttttat caaagtcaat tatagaaatt ggttcgataa aaaatgctca
    13621 aaagtgggta taaaaattcg agaatcttta aatccaaata tatgaatact tataaaaata
    13681 aaccgtttct gcatatagga tgagagcgat taaaagtatt tgatctccaa tattaagttt
    13741 gtgtttaagc tgaaaaacat aatgaaagtc agcaaagaaa agttatgaaa aaacaagaaa
    13801 gtttgatcat tatatcgttg atttagttac tgtcacgcat ctataactga atatttcgtg
    13861 ttcaattgta actgttataa tctttttccc agtcgtcact actgataagt agtgacgact
    13921 ggggagcatg gccactgttt acgcatatca aaaagggcgg tcgccacccg ttcgggatgg
    13981 ctctgctact ccctgaaaaa ttcagatggg agctggtagt cagagggaat aagttttgag
    14041 atttagattt tttctggtcg ctcacactcc gttcgctttg tacttttctt tttgacttaa
    14101 acaggtttaa accgagcgag tgtctacgag cgattgagca ttataaaaat ttcgcccctg
    14161 ccttttccct gattacaagc tccctttcga ttttaaacgg tagatacaga gcatcccgaa
    14221 tgggtgcgaa caaacttaat ttgaatctag accgagcgaa gcgagtaaaa aagcttatga
    14281 gcgaagcgaa ttccgagttg cttttgcttt ttcttaaagt cacgaaaata ttaaccaaaa
    14341 aattacccct cgaatcgagc gaaagcgaga ttcaatagag tttgagcgaa gcgaaaacca
    14401 agggtaattt ttcattccct gtgcttttaa ttattttaaa ttttttaaat gcttttagat
    14461 ggcttgaaag ccttatgaat aaagggtttc atctcttata tgtccacgtt taccttgcaa
    14521 tatgtccacg tttaccttgc aatatgtcca cgtttacctt gcaatatgtc cacgtttacc
    14581 ttgcattagt acacaaataa tattaacgtg tatttataca caataaaaaa atagtggct
//
