LOCUS       CP137453                8828 bp    DNA     circular BCT 08-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 2022JQ-00839 plasmid unnamed3, complete
            sequence.
ACCESSION   CP137453
VERSION     CP137453.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN34174867
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 8828)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/25/2023 12:49:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,791
            CDSs (total)                      :: 5,670
            Genes (coding)                    :: 5,528
            CDSs (with protein)               :: 5,528
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 53 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 21 of 142
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2022JQ-00839"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022"
     gene            join(8656..8828,1..322)
                     /locus_tag="QDT99_28900"
     CDS             join(8656..8828,1..322)
                     /locus_tag="QDT99_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPA01066.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVQAEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPEGKDNFLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     I"
                     /name="hypothetical protein"
     gene            complement(710..850)
                     /locus_tag="QDT99_28905"
     CDS             complement(710..850)
                     /locus_tag="QDT99_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WPA01067.1"
                     /translation="MQEPPVQPDCCALPGNYRLESNPGRHDKAPLAAAIGNWEFAENLF
                     S"
                     /name="replication initiation protein"
     gene            1210..1572
                     /locus_tag="QDT99_28910"
     CDS             1210..1572
                     /locus_tag="QDT99_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WPA01068.1"
                     /translation="MAWHSVSQAQNMTGKSRRTLYRDMASGRLSWRTNTSGHRELETSE
                     LIRVYGEIKNDGTAERHTVSQPDVTVSGSDIRDELRALRQEVADLKSMLLRLEHKQEEQ
                     PETAAPWWRFWQKKST"
                     /name="entry exclusion protein 1"
     gene            complement(1825..4308)
                     /locus_tag="QDT99_28915"
     CDS             complement(1825..4308)
                     /locus_tag="QDT99_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WPA01069.1"
                     /translation="MKKNILAGIFFVAMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDHQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYRAQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAAVPAVALALML
                     IFMPFLPSWVLYAAALAVTFAIYSRSKKILGGLASRCGRFPALASGLRWMGGFVSGLDA
                     DRKQETGAASVNPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDGNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFVRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGGG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYTGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(4356..4658)
                     /locus_tag="QDT99_28920"
     CDS             complement(4356..4658)
                     /locus_tag="QDT99_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WPA01070.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYQQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="mobilization protein"
     gene            4818..6641
                     /gene="mobQ"
                     /locus_tag="QDT99_28925"
     CDS             4818..6641
                     /gene="mobQ"
                     /locus_tag="QDT99_28925"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WPA01062.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGIIHDYTRK
                     SGVEHSEIFLPQNSPDWASNRETLWNAVEQKENRRNSTVAREFELAFPAELDGKQRLEL
                     LRDFCSSLVERHGMAVDAAIHAPGKEGDQRNHHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNHWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAKLDRVPTIH
                     IGYAAAQMEKRGLETERGNEYRQIILRNAEIQPLSRAIQLIDNWRATHDNNSRAGHGSI
                     APVPERDKGRDKRGVRTAQSYTGRVGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKSKRPEVIRLEWQSLRRDKLTFVQDKAGRIAARGA
                     AHVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRDYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETLSEETKELESGE
                     KEKLSKRDQVLEKFKAKVERDKQNERGGRGR"
                     /name="mobQ"
     gene            6784..7203
                     /locus_tag="QDT99_28930"
     CDS             6784..7203
                     /locus_tag="QDT99_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WPA01063.1"
                     /translation="MSEEQRRRGFMKAEASLRLEGIDPSGSPRYESVKARIISGEITYD
                     QGRAEILAFHTGKRAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRR
                     LSAEESYKLAEQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(7364..7717)
                     /locus_tag="QDT99_28935"
     CDS             complement(7364..7717)
                     /locus_tag="QDT99_28935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPA01064.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            8134..8652
                     /locus_tag="QDT99_28940"
     CDS             8134..8652
                     /locus_tag="QDT99_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WPA01065.1"
                     /translation="MSVRAQRAGKRNQSERRIELINTLWEGEEIETWDRNDRPRNDGFI
                     TVPRYLPLLGVLMDELSKGSPLSSTYLALWFRVSDEGLIEIRDKTVLALESGFASGRGV
                     TTWTGRMRKLKELGFISCREGSSGEFHNVLIVHPLVAVKKLLDEGKITKGKTYNTFAER
                     VIEVKSSWE"
                     /name="hypothetical protein"
     Typing          complement(742..1109)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 cagaggtgaa actcggcatt tctgccagga aagatacgaa ggaagtaaga ctatcgtgca
       61 gcggatactg tcagcgatag aaaacggaga gtacataacg gcctttattt ccaaggggca
      121 agggcagcgg tattaccacc ttaaccatca tgatgacttc attctaatgg agataagaaa
      181 gcctgaaggt aaagataatt ttcttcgcat acatgttgtt acggcatata cgctggatga
      241 atgggggacg gtcaacaaag gacggaattt gaggttcagg tatgttttgg agcagaggtt
      301 acaggggaag aaaatcatat aaaaaacaaa aagcgcccaa ggcgcctttg ttttactgca
      361 aaaatttcgg tctggccttt cgttatcccg ctcactggct aggagggttg cccccggcat
      421 cagcacccct ctttcaaggt tccggtttgt gctgacaggt atattgtcca cttaccaatg
      481 ccacttgtca acttcacggg tttgtcatga tcttcttgag atcctttgtt tatcgtcgta
      541 gtctcttgtc cagtaaacga aaaaaccacc cggcaaggtg gtttttcgaa ggttcagtaa
      601 accggcaagt ttctgaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc
      661 cttccagtgt agccgtactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca
      721 aattctctgc gaactcccag ttaccaatgg ctgctgccag tggcgctttg tcgtgtcttc
      781 ccgggttgga ctcaagacga tagttaccgg gcaaggcgca gcagtcgggc tgaacggggg
      841 gttcttgcat acagcccagc ttggagcgaa cgacctacat cgagctgaga tacctacagt
      901 gtgagctatg agaaagcgcc acatgtcccg taagggaaaa aggcggtcag gtatccggta
      961 aagcgacacg gtcggaacag gagagcgcaa gagggagcga ttcaccggaa acggtgatga
     1021 tctttatagt cctgtcaggt ttcgccgcct ctggcttgag cgtctgtttt tgtgatgctc
     1081 gccagggggg cggagcctat ggaaaaacgg gtctggcgtt tttctctttg ctttcttacc
     1141 gcgtgcctgg atgccgttag cctgctagag tgcagcccgt agagtgtgtc atttaatcaa
     1201 ggtggtgtta tggcatggca cagtgtgtca caagcacaaa acatgacggg gaaaagtcgc
     1261 aggacccttt atcgtgatat ggcgtctggc cgcttgtcat ggcgaactaa tactagcggc
     1321 catcgcgaac ttgagacctc agaactgatc cgtgtttatg gcgagattaa aaacgatggc
     1381 acagctgaac ggcacaccgt gtcacagcct gatgtcacag tatccggtag tgacatcagg
     1441 gatgaattgc gtgccttacg gcaggaagtt gcagacctta aatctatgct tttgaggctg
     1501 gaacataaac aggaagagca gcctgaaact gcggctccgt ggtggcgttt ctggcagaaa
     1561 aaatctactt aacccttttc tctttttcgc ggctaaagtt gattacgtta tccggtagtg
     1621 tttcaggctt ggtggtcttt tgtgcctcga ttggcatagc actgtgtctt ctgttttgcg
     1681 tcaccagagc ccagataaag gcacagacaa agcggaaaga gaagtatatc ggtatgccta
     1741 tcacaaaggc gacggcggtt aaggccggga gcaagacccg gccaaacatc agagctaaca
     1801 gaacgccgac aacgattcca aatatcatgc gctgtagtag gggttagggt ctgccatacc
     1861 cgtgtattct gggtctgtca gatagttaat acgtgtgagc tgataagggt attcgccttt
     1921 cagaatcaca attggtcggg ttggtccaag cctcattact tcgtcaggtg tcagtaattc
     1981 cctcccagca aactgctgac ttgagccaga tgaacgacca cgattcattg agccgccacc
     2041 cccggaaact ccgccgccag agttgcggcc tgaattctgg gtttcaaatt caaccgtcga
     2101 tttacccaag gtgtcgctga cgtattttgc agtgtcatag tcgtctgtgc cgtagaacgt
     2161 cttcgctgag ttggccagaa acgtctgcca tttagggtag gcacttttca gctgtgacag
     2221 atcctgcagg aagacccaga aagacagccc gtagccacgt aacaggctta cggcatcctc
     2281 gattgatttc atatagccca gcacgttgaa ctcatcgagg atgaatgcga cattatgagc
     2341 tggtttcgcc ttcgattctg ttacagcgga gatcacagaa ccgataaaca gccttacaaa
     2401 tcgggtattt gctgcaatgc gattagctgg tagcacaaga taaactgtca tcggttcagc
     2461 tttgatggct gacaggttga agtctgagtg tgacagcgca gctgctatac gcgggtcgtc
     2521 cagaaatgct gtgtttttac gggcggtaga caatactgag ccacgttccc ggtctgccat
     2581 ccccatgagc gtgttggccg ctcgggcggg gatgccgaaa gcaacgtctt cacttgcggc
     2641 catatcggcc agcagggcta acaggttttc ttcatcactg gtgatcagtc gacgtagttc
     2701 cgcgagatta cgtcgggact catccggtaa tccggaaaca tgcagcatca ggccctgtaa
     2761 cagggttcgg gctgattcat caaaatgatt accgtcaccg cctttctctg ataccacaag
     2821 gctttcggcc agaactgcag attcagcgac gcaatccggg ctggtcacat cgagacgatc
     2881 cagaatgttc actgattctt ttatgccgct ggttataccg aatgggtcta ttacatggac
     2941 gctctgaccc atttcacgac ggcgacgacc tgtcacctcg gcgttttccc cttttggatc
     3001 aaagacaagt gctgaaccct gataagtcag aagattggga attactgcgc caatcccttt
     3061 tccgctgccc gtaggggcaa cagtcacaac gtgcccggtg aaacagaatt ttccatccac
     3121 tccgggcggt ggggacagtt ctcgtgccag ggtgaatccg gcttgctttt ccgttggtaa
     3181 caggtggtta ttttcccgca tttctgctgc ggttccccag cgtgatgagc cgtgagtgtg
     3241 atgtgatgcc gggttgacgg atgccgcgcc ggtttcctgc tttctgtcag catcgaggcc
     3301 agacacaaaa ccgcccatcc agcgaagccc ggaggccagt gcggggaaac gcccacagcg
     3361 agaggccagc cctccaagaa tttttttgct gcgggaataa atggcgaatg ttacggccag
     3421 cgctgccgcg tacaataccc atgatggtag gaaaggcatg aatatcagca tcagcgccag
     3481 tgccactgcg ggtaccgctg cggccatgac tgctttcact gatgccgtga aagcatccag
     3541 ccttacccgc cgtgcggtaa gtgcaccaat aataccaacg atgataagga acagcagaac
     3601 tggagcatat acctggctta tccgggcaag gaagccgccg aaatatcctt ccacgctgtc
     3661 acctttgaaa ttatgggctt tatcttcgac aatcttatct gattgcgcac gatactcttt
     3721 ctgggcttcg ttaccggcgt tctgtgccac gccttggata aagctctgtg cccgaacccg
     3781 agtctgataa accagctgtt gcgcgatacc tgcagctttg acctgtttat ccggttcagt
     3841 cagcatttga ctgacgttat cagtcagtcc gtcagcgctg gccggagcaa cgagccctgc
     3901 attctggtac aggtaattca gatcaccgcc gaaaagcgcc aggatgttac ccgcgcccag
     3961 taacgatgaa tttttttctg ccagtgcatg caggctatta atctggggag caatgttatc
     4021 gaacgtggat tttacggcgt tccagttctg atgatcgcgg gcgctgcgga tggcgttttc
     4081 tgcatctccc atcgcctgga aattttggcg ccactggtca taagtgtcat taacggcctg
     4141 acaccgcttt gtttcctgta gtctggcgat gttttttagc tgctcatctt ggatcattgc
     4201 ccagactgtc tcatttggat ggcagcgggt gaatgtttcg gccgctgcgt gccatactgg
     4261 caatagcatt gcgacgaaga aaatcccggc tagtatattt tttttcacgc taaatctcct
     4321 gatctgattt atcatccggt aataaataat tggcgtcact tattaccggg gttaaaagat
     4381 tcttttctgg cgtcaactgc ctttaaaccc atttcatcaa acagtgcccg gtctctgtca
     4441 tttttcagct cattgtccag aagactgacc aactgctgat aattccattc cccacgcttt
     4501 atttttgcta gtacggctcc accgattagg atttttcggc gggtatcctt cttgcgttct
     4561 tctgtctgct ctctttgctt taattgctgg atttgcgcct ttatctgagc ttccttctga
     4621 cgcaactctt ccaatttttc cgctcttgat tttgccatta gtcacctcgt tgattcaaat
     4681 ttgaacgata acccctgtac tgatatattt caacccgaag cgaaagcaaa gggcgcactt
     4741 atgcaaactc ttcgagtttg cgtgcggcag ggttaaaacc ctgcacccaa ggtcaacgga
     4801 aaaacaggaa tacgaaattg gcaatttttc atcttagcgt taagacaatc agccgtagtg
     4861 ctggccgttc ggccactgcg gcaattgctt atcgtacagg ttcaagaatt gttgatgaac
     4921 ggactgggat tatccacgac tacacccgta aatcaggcgt cgagcattca gagatttttc
     4981 tccctcaaaa ctcaccagat tgggcgagta atcgggagac tctatggaat gcagttgaac
     5041 agaaagaaaa ccgtcgtaac tcaaccgttg ccagagaatt tgaactcgca tttcctgcag
     5101 agcttgacgg aaaacagcgt ctcgagctgc ttcgtgattt ttgttcttcg ctggttgagc
     5161 gtcacggaat ggcagtggat gccgctatcc atgctccggg taaagaggga gaccaacgta
     5221 atcatcatgc tcacgtttta tgcagcaccc gccgactgtc ggaggaaggt ttcaaggata
     5281 agacccgtga acttgatgat tttaagagcg gcgaagtcaa ccactggcgt tctgcatggt
     5341 cagaaatgac gaaccgtcat ctggaaaagg ctggccgttc agagcgtatt gaccatcgtt
     5401 cacttgaggc gcaacgggaa tcagctattt ctcaggggga ttcagccaag gcagcaaagc
     5461 tggatcgggt tccgactatt catatcggtt acgcagctgc gcagatggaa aagcgcggct
     5521 tagagaccga acgaggcaat gaatatcgcc agattatatt gcgtaatgct gaaatccagc
     5581 cgctttcacg ggcaattcag ctgattgata actggagggc aacccatgac aacaacagca
     5641 gagcaggaca tggaagcatt gctccagtcc ctgaacgaga caaagggcga gataagcgag
     5701 gagtccgcac cgctcagtcc tacactggcc gcgttggagc cgtcccgccg cccagcgcaa
     5761 ggggtcgcct gcgcaacctg tccgaactcg atatggttcg cttcggagaa cgaagtgaga
     5821 tgttattgcc gggcaatgta tctcgtgacg tggagcacca gagaaccgac agaaatcagc
     5881 ctctgcgacg ggaaaacact gtaaccagca agtcaaaacg gccggaagtt atccggcttg
     5941 aatggcagag tttacgccgg gataagctga ctttcgttca ggataaggcc ggacggattg
     6001 ccgcccgtgg ggcggcacat gttgaacgcc agagacaaaa aatatcctca catgagcgac
     6061 aaagaccaga acagcccagc ggcctgatag gaatgtttaa acgcagcgcc tatgaacagg
     6121 ccgccagtac ctggcagacc atcaaaaatg gcctcgatcg tcgtctggtg cagctgaaaa
     6181 atcgacttgg ctttgtcaga gactacatgc gcaagtctgg cgcttatgaa atgtctacgc
     6241 cgggagaacg gctggccgac cagctggccg cgaaagaaaa tccgatcttg gctgggcaga
     6301 taaaatcgct caatgctcag aagaacgcgg aagatatggc tgcataccgt acccgcgtac
     6361 agtcccgcaa acaggagaaa gctgatatga agctggaaac tatcgaagaa ctcaaacaac
     6421 gtggtgatgc ccggagagat aaagccgaca aagccgaacg tgcagagctg gaaaaagcgt
     6481 ctcatcaggc ttcactgcag aatgaaacgc tcagcgaaga gacaaaagaa ctggagtccg
     6541 gggagaaaga gaaactcagc aagcgtgatc aggttctgga gaagtttaag gcaaaagtcg
     6601 agagggataa gcagaatgaa cgtggtggcc gtgggcgtta gcggtatgca acacagcgcc
     6661 aaccatgctg catacctgac ccggccagac tgatcaggcg tcttttcggg ctgaatgcat
     6721 tgtactggtt aacattattt catgacatat gaggtacgcc tgatattgac atatgaggta
     6781 gatatgagtg aagaacagcg ccggcgtggc ttcatgaagg ctgaggcaag cctcagactg
     6841 gaaggtatag atcccagtgg aagcccccgc tacgagtcgg ttaaagcgag aattatttcg
     6901 ggtgagataa cctatgatca gggacgagct gaaattctgg ctttccatac cgggaagcgt
     6961 gcggagccga caactgcggg ggaaatgctc gcagaggaat ttttaaaacc tctgaacatg
     7021 tctcagggtg aactggcgca ggctacagga attcctgagc atcatatcga tgatattatc
     7081 agtaaccgcc gtcgcctcag tgctgaggag tcttacaagc tggccgaaca gttccagacc
     7141 gataaagatt tctggctcaa cctgcaggct ttgcatgacg catggctgac gcaccagaaa
     7201 tagcagccag gggcgcgatt agatatataa tgttccagtc taatgtagga tgttccatgc
     7261 aggtcagagt attaaaaata aagccccgtc cttaccaggc ggggctttat tttttcctgc
     7321 ccctcaagtg tcataatggt tctgaaatcc ggacatccaa aaatcatgta ttagccccaa
     7381 cgagtttctc cataccagtc atgaagctca ccagttccct gcggccatcc ggatattctt
     7441 tgataacgca gttttctggt gtcgtctcat cggcgtaata gacaggaacg ccgctgagaa
     7501 gagcttcgcg ggcggcttcg ccgcagtcat gttccagtag tgatagatta atggtcgcca
     7561 tgatgacttc tccttcggta atactccaaa tggatggttt tgaccctttg accttctttt
     7621 cttggctaaa ttgattgcca ttcatttgtg tgcatatcaa ttcataccaa caaaaactca
     7681 ccagtgcgat tttacggcct tttaattggg taatcatggt cttttgaggc ttagggcata
     7741 aaaacgcgct gagggcctct gaggcggttc tgctaacgga tgttatggta aatacgagtg
     7801 accggcatgg tgactcttaa tttatttttc gatttctcca cccctccttg ccccctacaa
     7861 ccccatggag cacaagggag caccggaagc acatggagca cggtgagcac atggagcacc
     7921 gcaattaaat aaaaactctt tttaaatcat gttaatgcga attctttttg taatatatat
     7981 tacttataac tgccgttttt gtaataaata ttgcattttt tatggttaaa aaaaataaaa
     8041 actggaagta attgtccgag ggaatttata aaagttattg atttataagg attttttggt
     8101 aaattggctc tcatgcatta acaggaggcg agtatgtcag tcagggcaca aagagcaggg
     8161 aaacgtaacc agtctgaaag aagaatcgaa ctgataaata cattatggga aggggaggaa
     8221 attgaaacat gggatcgtaa tgaccgacct cgaaacgatg gcttcataac agtacctcgt
     8281 tatcttccat tactaggtgt tcttatggat gagttatcaa agggaagccc actttcaagt
     8341 acctacctag cgttgtggtt cagggtttca gacgaaggcc tgattgagat aagggataag
     8401 actgtgctgg ctttagaatc aggctttgcc agtggaaggg gggtaacaac atggacgggg
     8461 agaatgcgga aattaaaaga gctgggattc atatcatgcc gggaaggtag ctccggtgag
     8521 ttccacaacg tgttgattgt tcatccgctg gttgcggtaa agaaactact ggatgaaggg
     8581 aaaataacga aagggaaaac ctataacact tttgctgaac gggtgatcga ggtgaagtcc
     8641 tcatgggagt aagaaatggc aggtacggta tggaatgatg ttaatacagg gcgtgaagtt
     8701 ttctcgatga cacacctgca ccctcagctg cataaggttg aggtttctgg ggtgcaggct
     8761 gagattgaga ttagcttcgg gtttcatgta tttactgacc aaaagcaagt cggaaaggta
     8821 atgagttt
//
