LOCUS       CP137448                3580 bp    DNA     circular BCT 08-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 2022JQ-00762 plasmid unnamed3, complete
            sequence.
ACCESSION   CP137448
VERSION     CP137448.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN33193817
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 3580)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/25/2023 12:38:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,506
            CDSs (total)                      :: 5,385
            Genes (coding)                    :: 5,254
            CDSs (with protein)               :: 5,254
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 51 of 131
            Pseudo Genes (incomplete)         :: 88 of 131
            Pseudo Genes (internal stop)      :: 11 of 131
            Pseudo Genes (multiple problems)  :: 15 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2022JQ-00762"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022"
     gene            2..268
                     /locus_tag="PZF77_27495"
     CDS             2..268
                     /locus_tag="PZF77_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WPA06322.1"
                     /translation="MAYKNKSGQPRERAMVAAYSLVTQFGGKQQDVAKVLGCSPSTVHQ
                     WVKEIGFKKEIAGLKQELSDANEYIEELAENLGLEYHSDDQED"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(514..795)
                     /locus_tag="PZF77_27500"
     CDS             complement(514..795)
                     /locus_tag="PZF77_27500"
                     /inference="COORDINATES: protein motif:HMM:NF016875.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WPA06323.1"
                     /translation="MDVQFKSKAYEKFISTGSTAGINQSFAKKLRAIYHTFSSAIELND
                     ISSVKYLEFHELKGDRKNEYAVSINGPWRVVFKTDGKTVYDVDIENYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1107..1379
                     /locus_tag="PZF77_27505"
     CDS             1107..1379
                     /locus_tag="PZF77_27505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WPA06324.1"
                     /translation="MHPGEYIREVYMEPFNIHAADLAKYLEVSPSTLSRILNAKADLSV
                     DMAFRLSKVLGRSPESWINLQTQYSLDKARKESADNFSVLRPIPL"
                     /name="HigA family addiction module antitoxin"
     gene            2149..2340
                     /locus_tag="PZF77_27510"
     CDS             2149..2340
                     /locus_tag="PZF77_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WPA06320.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2340..2762
                     /locus_tag="PZF77_27515"
     CDS             2340..2762
                     /locus_tag="PZF77_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WPA06321.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1621..1992)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.783"
                     /coverage="91.0"
                     /evalue="5.8799999999999995e-176"
ORIGIN
        1 tatggcttac aaaaacaaga gtggtcaacc tagggagcgg gccatggttg ccgcgtattc
       61 tctggtaact cagtttggtg gaaaacaaca ggacgtagct aaggttcttg gttgctcacc
      121 atcgacggtt catcaatggg tcaaagagat tggttttaaa aaagaaattg cagggctcaa
      181 acaagaactt agtgatgcaa atgaatatat tgaggagtta gctgaaaatc ttggccttga
      241 atatcattca gatgatcaag aagactgatg agagttactt catcagtctt ctttcttaac
      301 ccataagctt ggggcatcag caggaactta aaagaataaa tgccatatgt tattgattgt
      361 aaaggaattt attaacaaat ggaaaaggcg cagatcttac aaactgcgcc taaaaagggc
      421 gaggaaccct ataacccaat catcaacacg tggattaagt attcatttga ataaagttac
      481 taatccatca cttggtggat tagtaaccta ctttcagtgg tagttttcta tgtcaacgtc
      541 atacaccgtt tttccatcgg tcttgaagac aaccctccat ggaccattga tgctgacagc
      601 atactcgttt tttctgtctc cctttagttc gtgaaactca agatacttaa cggaagatat
      661 atcgttaagc tctattgccg aggaaaacgt atggtagata gcgcgaagct tcttggcaaa
      721 actctggttg ataccagcag tcgaaccagt gctaatgaac ttctcatacg ctttactttt
      781 gaattgaaca tccatctttt tctccttctt tctttcgtcg ttttcgataa cacctgatta
      841 aaatgaaata catagttact acctcgattg tttgttggac taagtattga aaaagtactt
      901 gacacaacca ttcagcgacc tataatttaa atgcccttta agggtaagaa taggtcattc
      961 actacaactc tgaatcatag ttgttgtttt tatttctgtc aatataaatt ttcatttgcg
     1021 cattacgcaa tgcgcatcac ccaagcaacc ggcggttgct tttttatgtt ttaattgagg
     1081 taggtatgat taatgtgttc aatcctatgc acccagggga gtatatccgc gaggtgtaca
     1141 tggagccgtt caacattcat gccgctgatc tagcaaagta tcttgaggtc tctccgtcta
     1201 cgttaagtcg aatccttaac gctaaggcag atctgagtgt tgatatggcg tttcgcctgt
     1261 ctaaagtttt aggcaggagc cctgagtctt ggataaatct acagacgcag tatagtctgg
     1321 acaaagctag gaaagaatca gcggacaatt tttctgtatt acgaccaatt ccactctgac
     1381 cgagaaaagc catctttaaa gatggctttt ctacatttag gcttgagatc agtatttagt
     1441 catcgtaatc tcttgctctg taaacgaaaa aaccacccgg caaggtggtt ttttcgaagg
     1501 ttcagtaacc ggcaagtttc tgaaccaagg taactggcta atagaggagc acagtcacca
     1561 aaactgttcg tttagtttag ccttaactgg cgcataactt caagactatc tcctctaaat
     1621 cagttaccag tggctgctgc cagtggcgct tttgcatgtc ttaccgggtt ggactcaaga
     1681 tgatagttac cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
     1741 agcttggagc gaactgcctt cccggaactg agtgtctggc gtggaatgag ataaacgcgg
     1801 ccataacagc ggaatgacac cggtaaaccg aatggcagga acaggagagc gcacgaggga
     1861 gccaccaggc ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
     1921 tgagcgtcag attctgtgat gcttgtcagg ggggcggagc ctatggaaaa acggccgggt
     1981 cgcggccttt ccctttatct tttacgtgat agctttgttt tctcgagctc ttatacacat
     2041 gttcctgccg ctcgccgcag ccgaacgacc ggagcgtagc gactgagtga gcgaggaagc
     2101 ggaaaagaga ctggtttgac actgagcact gacgctctga ggccctttat gtcgaaacaa
     2161 cagaaaacag cgctgaatat ggcaaaattc atccagaatc agtctctgct cctgctggag
     2221 aagcttaacg agcttgacct cgatgtcgag gccgaccttt gtgagaaact ccacgatgat
     2281 gcggagcatc ttttccgtac actgtcctcg aggctggatg aattacagga tggtaactga
     2341 tggccagagt gtctattagt gaagcggcac ggcttgttaa ggttagtcgc cccacaattt
     2401 acaagatgat taacagtggg aagttaagtt acacctctgt tgtaaagcac gggaaggcta
     2461 ttaaagttat tgatacttca gagcttatta gagtgtttgg ttctcttgat ggtgtcatcg
     2521 atactgtaaa atatgatgta aaaagtgacg cagaatctac aggtgttaac agcgttggtt
     2581 tacatgattt acaacataga atcgctttac ttgaagctga aaacgatggt cttaaagggg
     2641 ctgttaaggc tcgagatgag catattgatt cccttcgtca ggctatgcag cttctggagc
     2701 acaagcacga accttcttca ccaccccatt ccccgtggtg gaaattctgg aaaaaatcct
     2761 gaccccgtca tgcttccgtt ttcctctccg gccatcccgg ggagttcctg aaccttccca
     2821 aaaatcgtca gatctctgaa cgctcctcac cactaaaaat acccacaccc cacgcaaaat
     2881 taaaattttg cagataactc attaatatca ggtagttggc tgttgttaaa ttacgcagta
     2941 ttaccttctg taaggtaaat ccaagtcgtc ggcgattcgc gcacgacttg ccgatgactc
     3001 gtcacagagt catcgacgac tcagcccaga ctatcagtaa cttatgacga cttgactcta
     3061 gagtcgaatc gccattttgg tgtttttttg accttggcga ctctagagtc aagtcacgag
     3121 tcgaatcgcc gatgaatcgt gtgctaagtc gtccggtgac ttgccatcac gtcgctgcca
     3181 gctatcgctg aaccggattc ctgaacgtgc taaaaacggg gtttccggct gcgccggcaa
     3241 cgcctcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggaag agaagagcgt
     3301 cggaaacagg acttaccctg gctgaatggt gtggatttcc gggttatgca cttgctcgca
     3361 tactcatgca tgacgtaaaa acagagcctg tgctttctgg cgggttttcg ggtagtttgt
     3421 tgccggtttt accggtttcc cgtcagaaac gcccttaggc cgttttagcg gtgcgtacaa
     3481 ttaagtcgtt atgttaaata ccatctttgc gtaactatct tgcataaatg caccgcctct
     3541 tttaaaatta aatttcgaaa tttaatttta aaagaggcgg
//
