LOCUS       CP137013                5596 bp    DNA     circular BCT 31-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 015710 plasmid p2_015710, complete
            sequence.
ACCESSION   CP137013
VERSION     CP137013.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN09842395
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Lin,J., Wang,J., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 015710
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Lin,J., Wang,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/24/2023 14:54:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,671
            CDSs (total)                      :: 5,548
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 47 of 131
            Pseudo Genes (incomplete)         :: 86 of 131
            Pseudo Genes (internal stop)      :: 14 of 131
            Pseudo Genes (multiple problems)  :: 16 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="015710"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_015710"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-08-07"
     gene            join(5578..5596,1..308)
                     /locus_tag="D0S72_28305"
     CDS             join(5578..5596,1..308)
                     /locus_tag="D0S72_28305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90859.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            334..879
                     /locus_tag="D0S72_28310"
     CDS             334..879
                     /locus_tag="D0S72_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90860.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            945..1295
                     /locus_tag="D0S72_28315"
     CDS             945..1295
                     /locus_tag="D0S72_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90861.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1365..1631)
                     /locus_tag="D0S72_28320"
     CDS             complement(1365..1631)
                     /locus_tag="D0S72_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WOP90862.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1615..1839)
                     /locus_tag="D0S72_28325"
     CDS             complement(1615..1839)
                     /locus_tag="D0S72_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WOP90863.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2506..2718)
                     /locus_tag="D0S72_28330"
     CDS             complement(2506..2718)
                     /locus_tag="D0S72_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WOP90864.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2725..3234)
                     /locus_tag="D0S72_28335"
     CDS             complement(2725..3234)
                     /locus_tag="D0S72_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WOP90865.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(3269..3973)
                     /locus_tag="D0S72_28340"
                     /pseudo=""
     CDS             complement(3269..3973)
                     /locus_tag="D0S72_28340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3912..4256)
                     /locus_tag="D0S72_28345"
     CDS             complement(3912..4256)
                     /locus_tag="D0S72_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WOP90856.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4246..4422)
                     /locus_tag="D0S72_28350"
     CDS             complement(4246..4422)
                     /locus_tag="D0S72_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90857.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            5062..5220
                     /locus_tag="D0S72_28355"
     CDS             5062..5220
                     /locus_tag="D0S72_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WOP90858.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          complement(4509..4585)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4774..5171
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2391..3922)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcatgctaga tataggatta aagcaatcct tacaacggct ttcgaataaa accaaaatga
       61 gtctaagtga tcttattaac cagagattat caggttcact ttcagttgaa gaacgcattg
      121 gcgaaaagcc tgaatggatg agaaaccttg atccctatct cgccaatcac ttttcaaata
      181 ttgagttacc gctcccttca ccattaaaag aataccaaga aaaaatggat gagatagatt
      241 caattattaa tgacatgatg gttctaaagg aatatttcag agctaagttt tacgacgaaa
      301 taaaataggc acgctatacg gaggcggaga attatgtttt ttaaacggta tttaacggag
      361 aaggaaatat acaaggcagc actcccaatt gttaggcttc aagggagagt taacacttca
      421 ttacgttttt tatccaaata ctcagatgta aacaaaaatg gattaattgc attgcatctt
      481 aatttattac attttatctc atacaacata cacggtgtag agtctctgaa aatcactcaa
      541 gcaggcataa acaaagctgt acaacaaagc cctcacgcag atgaaataaa aaatattgcg
      601 caggcaatag gacgcaaagg acctgagtac atcgaaggat taattagaaa cccatggggg
      661 aagaaaaatg ataatccact aatatttata atgagcattg aagaaagtat aaaagaaacc
      721 cttaactcag gttatatgtt atatagagta gctcccataa ctgagggtga agcagctgct
      781 ttcatggatg ttcttaaaca attattatta tttgaggata attttcgcct ttttggtaga
      841 gacttggttt ataaaataga aaaattaaaa atcagataac aaagtatggt gaacgaaaaa
      901 tgaaagagag gatagtgaaa tgtgtaatac caagattctc aactatgtat tggttaattg
      961 cgttcctagc atttatattc cccggtcgat cttttgctga cggttacacc gcttactacc
     1021 gatgtaatgg tactcaggaa gcatctctta ctgttaaaga taacaaacta agtatggttg
     1081 taggtctttc taagtttaat aggcaactgg atacttatag attcaaagaa aagggaatcg
     1141 aagtagtaat gcagagagct gcttctgatg ataactctga ggccataatg tcttttatta
     1201 tgcctgacgt taacaatatg accttattag ttaaaaataa cggctacatt atcgttaaga
     1261 caacctgctg gttaactaaa accgacaaac agtagaacac gtagttagtc gagtatttac
     1321 cataatacct gttgtcggca tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg
     1381 cggcgatgtc caatcgtggc ggcaaggata acaagctcgt catctcggat ctcacacaga
     1441 acgcgataat ctcccacccg atagcgccag aactcgccca gctcaccttt gagcggcttc
     1501 cctgactggc gaggatcggc agcaactgca atgcgtaaac tcataaaatc cacaatccgt
     1561 cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg cccgatcgga atagttaatc
     1621 gtccagacca agtgacttcc tcacgtcttc agacgaatgc actgcttcat caccacggcg
     1681 aacgcgtgca gcagtttctg ctgaaagata ataatcctcc aggtcttcca gatgcgccag
     1741 tattgcttcc cgcgcataaa acgtctttgt tctgccggtt tgcttcgcca gcgagtccag
     1801 acgggactca atctcatcag ataaccggat agcaagcata taaacctccc gaacaccatt
     1861 tgctatacat gtatatcaac agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt
     1921 tagcccctct tgataatccg taacgcggtc aatatatcta gcttgagggc tttcctctta
     1981 actattgaaa ggataacgat tatgggtctt aagccgggtc cgaaacgaat tgccgattca
     2041 acaggggaac ctgataaacg ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa
     2101 ttgaaaccca gcaaatcttc aaaaaaataa gtcagtagtt aaaagccctc cggggctttt
     2161 ttgtatctgc cgttcacgct gattaaacat aacacccgtt gcccgcaccg ccccggacgg
     2221 gttcgccgcg aacggggtgg cagtcaggta aaaccggcaa caaaccaccc gataaaccgc
     2281 cccgttcgcg acagataaat tttacctggc tgcatagcca tgcagccagg tgaatgacat
     2341 ccggtttcag cgtaaatgcc cgatgagaac cctgttttcc tgcatttcag cccagcgacg
     2401 gaccgtcata gcttcgctgc cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt
     2461 gctcgtgcgc ccgttctgcg gccacggcca tctcatgacg ctgcactacc ggctcaaacg
     2521 ctgcagttgc ccgctcaagc gttccacctg ctgactcaag cgcgtcacgc gctcgctcag
     2581 cgccgcgttc tcccgttgca taagccctga cattttccgc cattccccga aggcgctctc
     2641 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     2701 tttttccagc tcggtcatcg gcacttactc cttcaccagt ctcagcgcgt tctgcttcga
     2761 ccgcccgttc ccgaccgtcc agctctgcgt cctgtctacg gccgtcccct tcggcagcac
     2821 cagaaaacgt ccgttgctgt cttccaggta cgtcacgccc caggtcttcg cgttcagctt
     2881 cgacagcgct gcgttctgcc ggtcgatttc ggccagattt gacgctatca agctcccctg
     2941 ataccacaac accccgctca ggctggcgaa cagcagtcct gacaccatcg tgatggtcag
     3001 ccatgtccgc ccgaccatac gcatcacgct caggcggttc gactgcatgg ccgcgctcat
     3061 accctgctcg tgggcgcgga tggcggcgct gattctcttc ccgctttcgc tcagttccgc
     3121 tctgacagac ttctcgtgct cgctgaatgc ggctttcagc atttcgccgg tactctgctg
     3181 ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg
     3241 ctcccttaag tcggatgttt cgccctccgt ccgggtcggc aatgctgatg ctgctctttg
     3301 tggtgcgcac gacctcaaaa cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt
     3361 taagctcccc tgacgaggcg agggccagta agccccgcgt aatagcctct gcggcttcct
     3421 gcttcgtttt gggtaacgaa gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg
     3481 ggtcgtgcag ccccagtcgc ccgttcacca cggtctgcca cgcatcgatg cgcggacggt
     3541 ctgcccggtc gtagtacggc tggagacgcc tgccggtcag cagttccgtg ttcggtacca
     3601 gaaaattcag ttccagccgc cccttgtccc ggtgttcaac ccacagcacg ctgtactggt
     3661 ctttatcgag tccgggcatc agaacccgct cgaagctcgc catcagcttt tcacgctggc
     3721 cgggcggtaa atcctgttca gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg
     3781 gcgaggcgtc gataagctcc cggacctcct ccggctttcc ctgcaggacg atggccccgt
     3841 cgcgctggcg gtctttcccc agcagataat ccaccggccc ggcaccgccg cctcgccctc
     3901 gcggatgaaa cttaacgatc atcgtctgcc cccttttcca gcacggcatg ccgcagccgc
     3961 tcgagttcgg catcaatggc catcagcgcg gccaccacct gaacccggtc agcgcctgac
     4021 cactggccgc cgttaatttt ccgggcgatc tggttgaggt tgttccccat cccggcaagc
     4081 tggcgcagca aaaccggatc gatggacggc agccgcaacg accgcgccgg gcgcgtatcg
     4141 agacaggttt cccgcatcca caccgccagt tgccttccat tgcagcgctc cagcagctgc
     4201 tggtactccc catcggtcac ccggatacct aaaaaacggg tacgcttaac cgccatcctc
     4261 tcctccgata tccgcaaaat gtgaccgggc tgaacgctca agctctgcca tctgctcccg
     4321 gcggtaatct tcctcctgtg cctgacgcat cagatttgcc atcactgccg gatgccagga
     4381 tctttttttt acgacgagcg gcttcttctt cggcttctgc attatccctc cttcgcaaac
     4441 gccggacggc acggaaaagt tttccgggtc gctccggcgt tggacaagcg ccagcgcgtc
     4501 agtggtgtta gcgggtttcg gggcgcagcc ctgaaccagt cacagcgttt acgctgtgta
     4561 tactggctta ctatgttggc atgagagcac gattcatgaa agtgcctcat gtggcaggtg
     4621 tgatatgtgg ctcaggagcg tccgcaatac catgcacgga cagatgaaat gacgcctcct
     4681 cgctcactcg gtagctgcgc tcctgccgtt cggctgcggc gggcgtgacc gactcacgga
     4741 taacgggata aagcaaaggc gctcaaatca gaaagggccg gagaaaagga tgcggcgttg
     4801 ccgtttttcc ataggctccg cctcccctga cgagcatcac agaaatggac gctcaagtca
     4861 gaggaggacg aaacccgaca ggacttaaag atgccggtga tttctctccg gcggctccct
     4921 cgtgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc tttttccctt
     4981 tcgggaagcg tggcgctttc tcatagctca cgctgctggt atctcggctc ggtttaggtc
     5041 gttcgctcca agctgggctg tatgcacgaa ccccccgttc agcccgactg ctgcgccctt
     5101 tccggtaact atcatcttga gtccaacccg gacggacacg caaaaacgcc actggcagca
     5161 gccattggta actggatata tacgagagag atattgagag gtcttgaagt gggtgcctaa
     5221 ctgcggctac actgaaagaa cagttttggt atctgcggtc tcgtataccc agttacccgg
     5281 ttaagcagtt ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc
     5341 gtttacagtg caagagatta cgcgcagaaa aaaaggatct caagaagatc ctttaaatga
     5401 tcgtcagttc tgttatcccc ggagctccat ccatcccacg gaaaaacacc cgccactgat
     5461 gccctcagcg cactgcacat catgtacaaa ccactccctc cctccttttt tattgcgttt
     5521 cttaatgcgc aatggtagct ttgtgcgcaa caaaaaacgc aatagcaagg gggggtaatg
     5581 agagaaagaa caaata
//
