LOCUS       CP137012               10060 bp    DNA     circular BCT 31-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 015710 plasmid p1_015710, complete
            sequence.
ACCESSION   CP137012
VERSION     CP137012.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN09842395
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Lin,J., Wang,J., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 015710
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Lin,J., Wang,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/24/2023 14:54:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,671
            CDSs (total)                      :: 5,548
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 47 of 131
            Pseudo Genes (incomplete)         :: 86 of 131
            Pseudo Genes (internal stop)      :: 14 of 131
            Pseudo Genes (multiple problems)  :: 16 of 131
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="015710"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_015710"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-08-07"
     gene            complement(625..774)
                     /locus_tag="D0S72_28240"
     CDS             complement(625..774)
                     /locus_tag="D0S72_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WOP90848.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(859..1116)
                     /locus_tag="D0S72_28245"
     CDS             complement(859..1116)
                     /locus_tag="D0S72_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WOP90849.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1126..2811)
                     /locus_tag="D0S72_28250"
     CDS             complement(1126..2811)
                     /locus_tag="D0S72_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WOP90850.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3138..3383
                     /locus_tag="D0S72_28255"
     CDS             3138..3383
                     /locus_tag="D0S72_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90851.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3657..4028
                     /gene="tolA"
                     /locus_tag="D0S72_28260"
     CDS             3657..4028
                     /gene="tolA"
                     /locus_tag="D0S72_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WOP90852.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            4025..4390
                     /locus_tag="D0S72_28265"
     CDS             4025..4390
                     /locus_tag="D0S72_28265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WOP90853.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4565..4843
                     /locus_tag="D0S72_28270"
     CDS             4565..4843
                     /locus_tag="D0S72_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WOP90854.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4831..5145
                     /locus_tag="D0S72_28275"
     CDS             4831..5145
                     /locus_tag="D0S72_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WOP90855.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5309..5737
                     /locus_tag="D0S72_28280"
     CDS             5309..5737
                     /locus_tag="D0S72_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90843.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5763..5942
                     /locus_tag="D0S72_28285"
     CDS             5763..5942
                     /locus_tag="D0S72_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WOP90844.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5969..6499)
                     /locus_tag="D0S72_28290"
     CDS             complement(5969..6499)
                     /locus_tag="D0S72_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WOP90845.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6506..7237)
                     /gene="mobC"
                     /locus_tag="D0S72_28295"
     CDS             complement(6506..7237)
                     /gene="mobC"
                     /locus_tag="D0S72_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WOP90846.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7237..9201)
                     /locus_tag="D0S72_28300"
     CDS             complement(7237..9201)
                     /locus_tag="D0S72_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WOP90847.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7811
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..522
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6510..7237)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ccagagttca cataaacaga cgctttttcg gtgcatctgt gggagccgtg aggctcaacc
       61 atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc gtttccggta
      121 ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata cctgtccgcc
      181 gttctccctt gcgggaagtg tggcgctttc tcatagctca cacactggta tctcggctcg
      241 gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca gcccgactgc
      301 tgcgccttat ccggtaactg ttcacttgag tccaacccgg gaaagcacgc aaaacgccac
      361 tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt
      421 gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct
      481 ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac
      541 cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat
      601 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga
      661 cgatggtgcc accgttccgc cctgaacatc ccggatataa tttgcctgac atgcggccag
      721 aagaaagcca gcaacaatca atataaaaag aaaaatagtc tttacctttt tcatatactt
      781 tccctgtatt taaagactcc ggcattcatc cctgaacacc ggaagtcagt atttcaaatc
      841 atccttgata attttttgtt accagttacc gtcccgataa tcaaaagcta tataacaatt
      901 atccacatca ggttttattt gatttttaaa aaatggctgt agtaaaggaa cccattcttt
      961 cttcaaatca aactcaccat tattaatatt atcctttaaa ggcattccca agctttcaat
     1021 gacagaacca tcgtcaccaa agtctttaga atactcttcc cctttaaact cttcagtttt
     1081 cttatcaaac catgtcaaac gtaatttaag ccccatattt acctctcaaa gatatttttt
     1141 aatgttccgt tttggatctg gccctttaac ctgcttaccc gttttgggat caaatgcgcc
     1201 taggtgttcg ccatcactgg cacgataccc ttcaagctca ccgtgctggg aatcccattc
     1261 ataaatttta cgttttttat cgccatacca tcgggcccgc ttaccaccac caccttgttt
     1321 aggtgtttta ggatcacctc gctttaactc tccaagtccc tttatgtctt ctgttttagg
     1381 tactggatga taatcatggc cgtagtcttt agtccccttg cgaggcttgt tcttttcctc
     1441 atttaactta ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc
     1501 cccggtaagt gcagccttat ctctggataa ggattcattc attgcgttta aagatgcctg
     1561 agccgctttt aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc
     1621 ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc
     1681 ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggagcgc
     1741 cttctctgtc gatgcaatgc ggctgtttaa ggtcgcaata tttttatctt cggcatccag
     1801 ctcagctttc gctttatcat agtctctttt cagcccctct tccggatgag cggcgtccca
     1861 tgcctgctgg cgacgctttt cttcctcctg acgctgtttt acctgtgccg gggtaagaac
     1921 atctgtcacc gacacataaa ccggcttctg tccggtctct ttcgggaaac gaataacggc
     1981 ctcacgggag ttaccaccgg ctgtaaaacc agccggacgt gaatcacctt tttcagcaac
     2041 aatgcctttt ggcggggctt tcgctgtcgg aacacctttc ggtacgctca cctgcagaga
     2101 cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac
     2161 cacagggaca ctcattggcc gacctgcgac aaccgcaata tgctgccgct catccttcac
     2221 cacatccaca acccgttgtc tgacacgaac cgtcgcctgg tccagtggta aagtacttgc
     2281 cggagtctcc gtcaccgcat cggccggtaa tgacgtcata atttttgaca tcattttcgg
     2341 atcatctttt gcaatctcag aaggcagcac accgtagatc gcaatccccc acagaccaaa
     2401 cttaaacggc cccttcaggg cagccagcac atcagcaacg gcggccgaca acgcatcgcc
     2461 ggaaactgat aaagccagtc cttcagcccc cggagtagcc agggcaggca gaccgaaggc
     2521 catcgcagtg gccgaagact gtccgccacc agagctaccg ctatgattgc cgctgttacc
     2581 accattacca ccgggtttac tcccgccgtt accaaactcc cccggattat agttatgaat
     2641 atctccattc ggcgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc
     2701 acctgatgaa gggccacctt taccagacgt accgttcaca ttcccactgg cctgaccacc
     2761 attatgtccc agacctgaat tacccggacc tcgaccgtct ccaccactca taccgttttc
     2821 ctctttgaca tttaaaacaa taaattaaag ccaacatact gtacatatac acagtataat
     2881 tattacctta ctcctcccgt tttcaggtgt caatatatga gattagttct catcttcagg
     2941 cacaaaaaag cgcccggagc cacctgtaag cagatcgcgt cttttcccga aaatttctca
     3001 tggcaacaaa ttttattatt taagacattc atcacactta catttcagat ggtatagaca
     3061 catctgaaag ctaactggca tgaggagagg acctgcggtt aaactgttcg ggaaacctga
     3121 gacgcccgga ggccactatg acgcacctga aactgactcc cgctgaagaa aagcgcctgg
     3181 cggacgcctt tgtcgctcag gcactggcca tgggcctgaa agggaacaaa acgctgccaa
     3241 aggcggtaaa acaacaggtg cgggaaaggg tgcttcgtgc cattcaggac gaacgtgaag
     3301 ccaggcgggc gcaggaaatg cgtcgggaga aatacgaggc tacggaaacc accttcacct
     3361 ggcagccaca gcgccgccgg taaggggtat ttaccataac gtcgcattgc ccgcaccgct
     3421 cagacggtct gagggcgttt ccggcggcta cgggataaaa caggcaacaa accacccgga
     3481 aaaccgccgg aaacgcgccc ggtaaattgt aacccgctgc atgcctatgc acgcaactgc
     3541 atggcgggcc gtttggtgta aaaacgatac gataagccgt gtttatgtaa atgaacgtgg
     3601 ccgtaaagag tgacaaatat acatgtaaca ttttttactg acaggtcatc aacccgatgc
     3661 gtttaatacc ctttatcctg ctggcattgt gcagcccctt actcctgacg gcctgtaagc
     3721 attccaccac ccagccgccg aaaatttata ctctggataa gtcatctaaa atcagtagct
     3781 acgctgcggc catcaagacc gctgttgaaa tgcagatgtt tgaaccagag gcctatgcgg
     3841 gaaaaatgtg caccgtgcgg atatccatac aacgtgatgg cactctgaac agcgccattg
     3901 ctgaaggagg agatccgaaa ctctgtaaag cagcaatatc agccgttacc cacgccaaaa
     3961 ttccgccggc accggatgaa aatacctggc aaatattcaa aaacgcccct ctggatttca
     4021 gaccatgaaa gcttttactc tgacagtgct ggcggccatg ttcctcactc cggtacatgc
     4081 gataccacta aaacccgtaa ccacactgca gccaacctac ccggtacagg ccgcagcaaa
     4141 acgcatcgaa ggtcaggtga ccgccacgtt tgatattctg gacgatggac gagtggacaa
     4201 cgtgtcgctt accggctgtc accttttttt tcgggaaacg ctgacggcaa tgaggcactg
     4261 gctatatccc gcagggcacc cggcaatgcg acaggttata aattttgaat tcagtctgaa
     4321 aaatgacggc ttacaggatc cgcgaccagt caccacccaa aagctcaatg atgatggttg
     4381 ccgccagtaa caggtccttt aacccgcagg ctgcaatagt cctggatgat gagtgagcct
     4441 gtagcagccc tgattagtgg aagcgaagct gaaaaatggg cagcaaaagc cgtatttctg
     4501 gcaaatagcg ccgccacgga ttaaagtagt catgtgtaat acatatgact acaaaggggc
     4561 ttttatgtca tcagcaacat ccatcaggct cgatgaagaa cttaaggacc ggctgaaaac
     4621 gctggccgat gaccggcacc gctcagcgca cgcgctaatg ctggaggcaa tcaccgaata
     4681 catcgaccgg gaagagaagc gcagccagta cctgcgggac ggacaggccg catggcagca
     4741 ctatcaggaa acgggtctgc accttactgc ggaagaagcg gaagcctgga taagtacctg
     4801 gggaactgag aacgagcagg atgctccgcc atgccacagg taacgatttc cgcgctggca
     4861 cagcgcgatt tacaacgcct tcaggacttt ctcaaaacca aaaatcggct ggcagccaga
     4921 aaaggaggtg aggtgatcgt ccgggctatc cagcaactga aaacattgcc agacattggc
     4981 cgcccggtgc cgtttctgcc gctggaatat aaggaactgg tgatcgggtt tggcgacagt
     5041 ggctatgtga tgctctaccg ccacgaccgg gaaatggacc agattgtgat cgtcaccgtc
     5101 aggcatcaga aagaatccgg gtatccgggg gcagacagcc tctaataacc gatgacgggg
     5161 gcgcaattca ttgcgtggcc gtcgccggtt tgacaagcgc agcgcgtcag aaaaccacat
     5221 cctgaacgga aaaacgggta aaattcagcg atacatttac cgtgagaaat tacacacctt
     5281 tcacacccga taaaatcagg ggtatacagt ggcgaaaaca ttgctcaacc tttcacaggc
     5341 agcacaagcc gcgggcatta ccagaagaac gctatacaat catgtaaaac agggaaaggt
     5401 taccgtgtca cgtgacggga aaaacaaccc tgtcgtggat gtgtcagaac tgatccgtgt
     5461 atacggaaat gtgaatatcc ctgagaaaca aattcccggc atttcacacc gtgaaaatac
     5521 acaaaaaaat ttcccacagg aacagcttct ggcaatgcaa aaggaactgg ccgatctccg
     5581 gcaggctgtc accctgatgc tggaagataa aacatcccgg gaagaagaac gccgccagca
     5641 cgatgatgag cgccggaaac tccaggccga agtggacaga ttgacaacag aactgacaca
     5701 gaagaaaaaa cgcttctggt ccggctggtt tagctgagaa cttaccgaag ggaatggaga
     5761 acatgacgaa gcaggaaaaa actgccctca atatggctaa attcattaag gatcagtcgc
     5821 tgctgttgct ggaaaagctg aacgagctgg acatggatac cccggccgac atgtgcgaac
     5881 ggcttcacga ggatgcggaa acactctggc aggtgatgcg ggataagctc ggggaagaat
     5941 aacagcaccg gccatgccgg tgctggcgtt attttttgcc gaacggccac caccggcggg
     6001 cgggtttgtc ctccggcagt tcaggcaccg gcacgttaaa atagcggctg ttatattccc
     6061 gcatcagctc cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca
     6121 cctcaatcat cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac
     6181 cgttctcgat ggcgaaccgc tccacctttc gatagaggta atcacacagc ttcattcctg
     6241 tgggtttgtc ccgttcccat tgcggatttt caccggtgaa aaagtacgtg gctttttcgt
     6301 cgctgttgag ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc
     6361 ggtcgacaaa acagaaaaca tacgccccgg tctgccaggc gagcaccacc atgtcttcca
     6421 gatgctcgct ggttggcata atgcagcgct cgccgtcttt gtcttccacg tcctcaataa
     6481 ataccatgta cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcgaaca
     6541 gcctctcccg gtccagctcc accgttttgc gggcctcgcc ggtctgtgga ttcacgattt
     6601 catccaccgc cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca gggcagatgt
     6661 accagatttc atcgatgccg ttcgcccggc ggttgaacag atacccggac accacttcct
     6721 gataccgcct gcggctcttg acggtgcgct ctacctcaaa tgccaccgtt ctgccgtcca
     6781 gctccgccag cgcgtccggc actttcatcc ctttcagggc catacggtgc aggtaccgcc
     6841 agccggtggc cccccggccc tccatggaga ggcgcaggga ctgcacggcc agccggtgcg
     6901 gcagctgcgc cgccgacacc cttgacggct gaaacggaat aacgtccgtc agcggctcgt
     6961 cctcgtcgaa cgagaacgcc accccgtgcg gcgtcagccc ccacacgctg acctgacggc
     7021 cgtcaaccgg gtatttcgcc cggaccagca gccctttcgc caccagactg tccaggaagc
     7081 ggtaaaccgg ctgtaccgcc gcaatcccca gccgtcgcgc aaccagctcc gccgtggaac
     7141 acagctcttc ccgcagccag cgcagggtca gccgtgtttt ctccgcgttc cgcgcctgcc
     7201 gcttcgcgtg gctgacgttg tactgctcca gtgtcatcag taaagccccg caaaatcatc
     7261 atccgctttt ggtccgccgc cgtcggccgg aggcaggtac tccgccagcg ggtccgtttt
     7321 cgccagcacc gggaaggttt tcagcgaaat ttcccggcgc tcaacccgca gcgggctgct
     7381 gaacgccagc cgcgccacgc ccagcccggt catcaccgcg aacccgtcag gcaggtgttg
     7441 cagggtgttc acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt cgccgctgat
     7501 gtgctccacg ttccccgcct cccggctggc ccggcggcgc tccacgtcca cccggatctc
     7561 cccggtctgg cccgccgccc actgcgcgga ctcctggctg gccgcgcgat agaaccagcg
     7621 gatcggcgtg ttatccagta ccgtggtttt cacaaaatca gcgggcagat cctgcccgca
     7681 ctgaccgaag tcgcccagcg actggtgcgc gagcagcagg ttgcagtccc ggctgcgcag
     7741 cgtccccagc gcgttcagca cgtacttcga cagcagatat ttaatctcat cgagcatgat
     7801 gctcgcgtgc ggccacctcc ggaactcatc ccgcgcaatg atgatttgcg cgcagcgggc
     7861 aaacagcatc ttctgcaccc ggatcaccgc ctcatcgtcc atcgagccga tgacgtacag
     7921 gcagccgccg ccgttgatta tccccgccac atcgataccg ctgtccgtct gcaggacgct
     7981 caggtcggcc acgttctcca gcttggtgat cagccccttc acgtcctttt tcagggcctc
     8041 cggcaacgcg tacgccgccg ccagcacctg ctgaatgttt gccccctgcg ggaactgctc
     8101 cgcaatcagc ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt
     8161 ctcactcagg ttaaacccgg caaccagcag attcttcagg gcgtactggt cgagatcgcg
     8221 gaacagattc agctgcgccg gtttccccgg ccgcagatcc agcagggtga agtccgggca
     8281 gtgcgcccgg aagacgtgcg gcgcccacgc gtcccctttg gggtcgaagt acaccacagc
     8341 gtccccgaaa tcgcgtacgc agcgaatgag cgcgttggtg gccatcgcgc tcttgccgct
     8401 cccgggcagg ccgaggatct gaatattggt cttgcgccag gtatcacgag ggaccagcac
     8461 cggctggccc tgctcatcct tacccagaaa caccgcctgc tgtttccggg cctgctccgt
     8521 ggaaaaatcg gccagcgcgt taaccggtac cggctccccg aaatgcaggt ggcggacgtc
     8581 tgtcatttct ccggtccgct ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac
     8641 cgcctcgtca gcggccgcaa aatgaccatc aagcagcagg aacgccagca gccgcgccag
     8701 aaggacgccg gcgacaagcc ctccggcaca ccaggctatc ccgaacaggt tagccggctg
     8761 cagcaggtaa taatctgcgt tcgcatagag gtccgggcgc aggacggttt ctccccagct
     8821 ctcgataaac acggcgacaa ccagcccccc cagaagggcc agaccggcag cgccggtcag
     8881 ccagtacgcg tagttccaga catgtcgccg tgccctgacc atccgcccgg gtgaatccag
     8941 cgaggccagc accgaaaatg gcacagtccg gttattatcg cgcttatagc gatcggcata
     9001 ctcccgccgc gccttatggc tcagccccac aaaacgcatt cggccggccc agagttcagc
     9061 cagaccgaaa aacaggccga taaccagaaa aaaaccggcc agactgtaat actcccggta
     9121 cagccagctg acccacgggt aaatctgccg ggccagctcc gggatatgcc actgccaggc
     9181 aagcgaatcc gtattaaaca tgcaatctct ctccttcaga cgacgggttt accggcgtga
     9241 tgcgcagccc ccgctgcaca agccgcggac atgccccaag gcatggcggc ggcggtcacg
     9301 tccgtggtga ttgcgttcag ctttcaccgc ggaggccagc ctctttccgc cccatgcgga
     9361 aaaggcgcag ccccggagga acaacgagca cgccccagcg tgtgagagga aaaggcggca
     9421 ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga gttcgccata gcgagcggct
     9481 gaccacccgc aaaatcagga gcagctttgc tgcttcgtca ggtgcgtcca gcaccgtgga
     9541 tttttgtggg tggtcaggcg tgagcctgac gggggccggg tgaatttctg gcgtggccgg
     9601 cgtccagccg gacagagcca gaaagagggg aaaagaccgt gcgtcggtgg cgtccagcca
     9661 gcgtaagcaa ggtcttttcc cctgaagtca gccacgcccc agcggggatg acttgcattt
     9721 ctccgcaagg agaaaaaagc aacagagata ggttaacgta aaatgttcag cgaaaaaccg
     9781 acatggttca cctatcctga taattgatcg tcagggaata gaaagactta atcaggggac
     9841 aatatcccac atcagcgcgt ccggtgaccg tcttgcaggt gccggggaaa ttgcgcctcc
     9901 tcgctcactc ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc ggacacatac
     9961 aaagttaccc acagattccg tggataagca ggggactaac atgtgaggca aaacggcagg
    10021 gccgcgccgg tggcgttttt ccataggctc cgccctcctg
//
