LOCUS       CP135474                9297 bp    DNA     circular BCT 03-APR-2024
DEFINITION  Citrobacter freundii strain isolateG plasmid pIsolateG_E, complete
            sequence.
ACCESSION   CP135474
VERSION     CP135474.1
DBLINK      BioProject: PRJNA1020811
            BioSample: SAMN37529178
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Lerminiaux,N., Fakharuddin,K., Mulvey,M.R. and Mataseje,L.
  TITLE     Do we still need Illumina sequencing data?: Evaluating Oxford
            Nanopore Technologies R10.4.1 flow cells and v14 library prep kits
            for Gram negative bacteria whole genome assemblies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Lerminiaux,N., Fakharuddin,K., Mulvey,M.R. and Mataseje,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg R3E3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-JUN-2023
            Assembly Method :: Trycycler v. v0.5.0; Flye v. v2.9.2; Raven v.
            v1.8.1; Miniasm/minipolish v. v0.3_r179
            Assembly Name                     :: isolateG
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 227X
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2024 14:38:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,127
            Genes (coding)                    :: 5,025
            CDSs (with protein)               :: 5,025
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 27 of 102
            Pseudo Genes (incomplete)         :: 77 of 102
            Pseudo Genes (internal stop)      :: 18 of 102
            Pseudo Genes (multiple problems)  :: 18 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="isolateG"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pIsolateG_E"
                     /geo_loc_name="Canada: Ottawa"
                     /collection_date="2019"
     gene            complement(join(8917..9297,1..9))
                     /locus_tag="RRL16_26165"
     CDS             complement(join(8917..9297,1..9))
                     /locus_tag="RRL16_26165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10004.1"
                     /translation="MSKSNIAALQALADNLLTVAMEESDPSHWAGGDKPTQELTKGERG
                     DRLWDKKSAAATWGLVKNIHTLIAQMNGDKPAAAANDDDEEESEHDATLRRAQEEAAEI
                     MRSLKEKYGDDMGATVGNITRRTKH"
                     /name="hypothetical protein"
     gene            complement(54..272)
                     /locus_tag="RRL16_26170"
     CDS             complement(54..272)
                     /locus_tag="RRL16_26170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10005.1"
                     /translation="MTYRISPRIKARQRWEALLNKGHRFALCLTQPDGGAGEVVMSARH
                     APTLQPFIKFRAGLMLVDVAKQLATME"
                     /name="hypothetical protein"
     gene            complement(269..442)
                     /locus_tag="RRL16_26175"
     CDS             complement(269..442)
                     /locus_tag="RRL16_26175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10006.1"
                     /translation="MQNVTPRAGIVQAQRIARAAFNQRVTQPVRSFQQWELAANLMRLA
                     TLAAQMTQEPAR"
                     /name="hypothetical protein"
     gene            complement(520..1224)
                     /locus_tag="RRL16_26180"
     CDS             complement(520..1224)
                     /locus_tag="RRL16_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WNT10007.1"
                     /translation="MKVIAYRRVSTASQTQSGLGMEAQQSYIGHAVKANEWELIGAFED
                     AGLSGGLAPHLRPGLKAAIELAKKEGAALLVAKIDRLSRSVADTAQVMEAVEIKVATMP
                     HANNFQLHLFAALAEQEKEFIQQRTREALAALQARADAGEEVATAKIARRNAVIGTVAT
                     AATRQKGADAMKTGADAFAKAIRAHILEAQHDGATTLQQIADALNGKGVSTRRGAKWEA
                     ISVSRVMKRIGL"
                     /name="recombinase family protein"
     gene            complement(1537..1707)
                     /locus_tag="RRL16_26185"
     CDS             complement(1537..1707)
                     /locus_tag="RRL16_26185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10008.1"
                     /translation="MKANTKANTFTGYRVSPTQPAADTPTDWHKTHRTAQVNPPSRNWT
                     RTPNRRSRFTR"
                     /name="hypothetical protein"
     gene            complement(1704..2189)
                     /locus_tag="RRL16_26190"
     CDS             complement(1704..2189)
                     /locus_tag="RRL16_26190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10009.1"
                     /translation="MSYADYEAEIHLRAGAALLADKQYPAALAAFCHFLNLPYSQQGST
                     DEQRDSQIASARKLVAYIESGIAEKVDEQKRQQAEAVRVALALEAEQKEAKRQRRARKL
                     WLREAVQPFIKSRASRAEIQGKLSATRGEVDIAIRWNRRNVPEFHGTGKATAQWGAR"
                     /name="hypothetical protein"
     gene            complement(2186..2698)
                     /locus_tag="RRL16_26195"
     CDS             complement(2186..2698)
                     /locus_tag="RRL16_26195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10010.1"
                     /translation="MQTPAAVARRAAELAEKKMYSRAASLMRTIITSPAASEAERDSAS
                     AMADRYTATLSAIRSKAEETRRANYEARRRYFAQLASSKPRTPNGLAMGFNTFANSPRP
                     AATEADPKRQPVKDTRAQAREKIKHVIELMRTGATPAQIRAATGLKARTLRHYMQKIRE
                     QQEGKPQ"
                     /name="hypothetical protein"
     gene            complement(3647..6178)
                     /locus_tag="RRL16_26200"
     CDS             complement(3647..6178)
                     /locus_tag="RRL16_26200"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase family protein"
                     /protein_id="WNT10011.1"
                     /translation="MDGIEPAENASPNAWLLETSAKMYANGWRIVPCWINPTDGLPAAR
                     YGYAKGQVYTPKHPGWNAPDTLKDIFAVILDDAFLLDYDGNKADEKKVISEPELAQLVG
                     VDLGTPHQVRESNNSKHWLFSLPPDFVPEEYKQSQDGRLAVGVDIKRGNQPVYIKAGKA
                     LPFGGTFPARTQLPVAPESLLAAMRKPVNVVRVECELAPCENSTNYGRRALEAACRKYL
                     ADCDAGRNSTLTAVAFPIFQLVAGGEIAEADAETLLTQTAEATGLEDGEILRVLANCKR
                     DGFKQPRRLNRADPDIFEDLSTPALDVFSDEAPQQGTPENSDDAAQPVKGKKLRAELSG
                     VKARGLELDEVMRLANMNKAYTHVIAGGKNLVISTSKRLVNNKWQTNFNPILKQELKGA
                     FEHVPAINGFTVTFNKDGEEVKKPGRALNIAEAWLTWPDHSFKPGGMDFFPNPKRCPDN
                     MLNLFLGWDVVPARGVSDAEIAPFLNLARDVICAGNAEHYTVLMDFFAHALQKPDEKPG
                     YAVVLVGEKGVGKGQFMRPLEYIFGAYFLETQGVEPLIGKFNADIERKLLCFADETRIT
                     TRAQEDGCKKFITSGRMRLERKGVDAIEINNFARLVAASNRRDAVRVTESERRWFMLMV
                     SDIVKQNTAYFAKYDAWLMTPGNIEKVAGWLMERDISAFNPFEAPHTDALKDAMLSSLN
                     EVNTFMLEELSKPVPFECRADAEQWGKVTPETVAAAAHFPTANVGLSAGGVLCETAYQL
                     YADYKTQNNPKDRTKGVWTRASVRMELATAFKDCGVEATGRKGSRVFYCPDFDEMRRAF
                     CKRNGLSLALTFPGVDESQPEGEQLTGADFA"
                     /name="primase-helicase family protein"
     gene            complement(6198..6785)
                     /locus_tag="RRL16_26205"
     CDS             complement(6198..6785)
                     /locus_tag="RRL16_26205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10001.1"
                     /translation="MARKPQPLYTRAQVEKRIDAEFGEAIKTSKRAKIARAALVEMLLG
                     KEVTRAEVHELTPAREDCCTAIDHIKQRWLVPVETVRIKNAGRVAIYRMQRVEIFRFMN
                     DNGRKVQRQQQELLVNAHKLVNKGKATCDFYRETGRQAPADAHAFASLVLPALSDGDAY
                     RAPETTIAQLVATFVTPDGLAPATETTTSADA"
                     /name="hypothetical protein"
     gene            complement(6790..7050)
                     /locus_tag="RRL16_26210"
     CDS             complement(6790..7050)
                     /locus_tag="RRL16_26210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10002.1"
                     /translation="MKNPTVIDTPAAAEMLELSRMAYFEHRRNAQNFPPSVPGYRRGTY
                     HACDMHAYAARYFSGLADKAEAEAAALRARARHFAELAQEA"
                     /name="hypothetical protein"
     gene            complement(7915..8472)
                     /locus_tag="RRL16_26215"
     CDS             complement(7915..8472)
                     /locus_tag="RRL16_26215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNT10003.1"
                     /translation="MSKTHITTVRLDDTTHANMLAMLDGNSASNFIRQLIAAEYARRQQ
                     PAAPSAQPVAPHDIQQQVDALRRQLEALAAHIGASLSAGGIEPETPEANGLASDPISPP
                     AAPAPDSQPDAVAAYPKPPKGGTPPAALARLISLKDMGASHVDCAAALNAEGFKRGTGI
                     AWDAQAVGVQWKKEQQRRSRKQ"
                     /name="hypothetical protein"
ORIGIN
        1 cttactcata tgtctaaccc ctatcatgtg gcggctaccg cgccgccctg ctgttattcc
       61 attgtcgcca attgcttggc tacatccacc agcattaatc cggctcggaa cttgatgaac
      121 ggttgcagcg tcggcgcgtg cctggctgac ataaccactt caccggctcc cccatcgggc
      181 tgggttaggc atagcgcgaa gcgatgccct ttgtttagta acgcttccca gcgctgccgc
      241 gccttaatgc gtgggctgat tcggtatgtc atcgggctgg ctcctgcgtc atctgcgccg
      301 ctaacgtcgc aaggcgcata aggttggcgg ctaattccca ttgctggaag ctgcgcaccg
      361 gctgcgtgac gcgctggtta aaagctgcgc gggcgattcg ctgcgcctgt actattcccg
      421 cccgtggtgt tacgttctgc atcgtgttgt atttcctttc cgggttttac tcacgccgcc
      481 ccttaccgct ccaacggtag ggggcaacct tctatattct tacaggccga tccgtttcat
      541 cactcggcta acgctaatcg cttcccactt cgcgccgcgt ctggtgctta ctcccttccc
      601 gttcagcgcg tcggcaatct gctgtaatgt ggttgcgcca tcatgctggg cttccaggat
      661 gtgcgcacgg atagcctttg cgaaagcgtc cgcgcctgtt ttcatcgcgt cggctccttt
      721 ctggcgggtt gctgctgtcg caacggtacc gataacggcg ttacggcggg caatcttcgc
      781 ggttgccact tcttcgccag cgtcggcgcg ggcttgcagt gctgccagtg cttcgcgggt
      841 acgctgctgg ataaactctt tttcctgctc tgccagtgct gcgaacaaat gaagctggaa
      901 gttattagcg tgcggcatgg tggctacttt gatttctacc gcttccataa cctgcgctgt
      961 atcggcaacg ctacggctta aacggtcaat cttcgccacc agcaacgcgg ctccttcttt
     1021 cttcgccagt tcaatggcgg ctttcagccc tgggcgaagg tgtggcgcta aaccgccaga
     1081 taatcccgca tcttcgaaag cgccgattaa ctcccactcg ttagccttta ccgcgtggcc
     1141 gatgtagctt tgttgcgctt ccatgcctaa gccgctttgc gtttggctgg cggttgagac
     1201 tcgacggtag gcgataactt tcatgggctg gctcacttgt attcgtttgt attcgtttgt
     1261 attcgcttcg atggaaagga atataacatt tcacggatgc ccattcaaga tttgttataa
     1321 actgtattcg ctttgtattc ggttgcgaat acatgctttg ttatatggct gtcgtttttg
     1381 gaagggtgcc agcccgttgg ggggttctgg cgtattcgtt ttgtatgcgc tttcgtattc
     1441 gctttcgtat tcggtgccgt tggaacgtag cgcaagccag cgccccggct gctggtggag
     1501 gatgtgccaa caagccaggg cgctgggcgc tcggtgctat cgggtaaacc ggctgcggcg
     1561 gttgggtgtc cgcgtccagt tgcgtgacgg tgggttaacc tgcgccgtgc ggtgcgtctt
     1621 atgccagtcc gttggggtgt cggctgctgg ctgcgtcggg cttactcgat agccggtgaa
     1681 tgtattcgct tttgtattcg ctttcatcgt gcgccccatt gcgctgtcgc tttgccggtg
     1741 ccgtggaact ctggcacgtt gcgacggttc cagcgaatcg ctatatccac ttcgccgcgt
     1801 gtcgctgata gcttgccttg aatctcggcg cggctggcgc gggatttgat gaagggttgc
     1861 acggcttcgc gcaaccatag cttacgggcg cggcgctggc gcttggcttc tttctgctcc
     1921 gcttccagtg ccagggcaac gcgcaccgct tctgcctgct ggcgcttctg ctcgtctacc
     1981 ttctcggcaa tgccggattc gatgtacgcc accagctttc tggcgctggc tatctggctg
     2041 tctcgctgtt cgtcggtgct gccctgctgc gaatacggca aattcaggaa gtgacaaaag
     2101 gcggcaaggg ctgcggggta ctgcttatcc gctaacagcg ccgcgccagc ccgtaggtga
     2161 atttctgctt catagtcggc gtaactcatt ggggtttccc ttcctgctgt tctcttatct
     2221 tctgcatata gtggcgcaac gtccgcgcct ttaagccggt agccgctcgg atttgtgccg
     2281 gtgtcgctcc ggtgcgcatc agttcgataa cgtgctttat cttctcgcgt gcctgggcgc
     2341 gggtgtcctt caccggctgg cgttttggat ctgcctcggt agctgctggg cgcggactat
     2401 tggcgaaggt gttaaagccc attgccagcc cgttgggggt gcgcggcttg ctcgatgcca
     2461 gttgcgcgaa gtaccggcgg cgggcttcgt agttagcgcg tcgggtttct tctgctttgg
     2521 atcggatagc ggaaagggta gcggtgtatc tgtcagccat tgcgctggcg ctgtcgcgct
     2581 cggcttcgct ggctgctggg ctggtaatga tggtgcgcat caggctggcg gcccgtgaat
     2641 acattttctt ttctgccagt tctgcggcgc gacgtgcaac cgctgcgggt gtctgcattc
     2701 tcatccctgc ccgttggggt tgtccggttt tggcttcgtg cgaaaaacgc ataacgcgaa
     2761 ggcacggagg ggagtttagt tggtttcgcc ccttcattct ccagctattt gatgtttgtt
     2821 tgaccaatat taaatttcag ccctactttt tttcgttatt gataggctgt gcatatcagg
     2881 tggctatctg attttcgccg gtagcgtgcg gggcaatttg aggggaagcg atgtaccccc
     2941 atcgttttgg gtgtacgggt atcggttcga aggggtgggg gtgtcggctg cggctggggt
     3001 gtacttggtc gggtgtactt ggtcgggggt atcaggtacc aagtacagca ccaagtcaca
     3061 tgaccaagta ccagcccgga agccttgatt ttactgggtt tcttacttcc tttactctct
     3121 tttgtacttg gtgtacttgg taagtaataa aatagctggg aatgaaaaag tgtggatggt
     3181 gggaaaatac ccaacgggct ggaattgtgg ataactcagc aacgataaaa atcgtggggg
     3241 aatttacccc cctaaccaag taaagcaggt acaccaagta caagcgcatg atacataaag
     3301 catttttgtg gtacttgctc tttgtacctg gtacttggtg caggtaccag gtagcaggta
     3361 gcaggtagca ggttgccgcc tgttgtatgc ttttcgtaca aaatcaggcg cattaatagg
     3421 cggtgtatat cgtggcacat agcgagttat acatagctga ttacttttta acctgtggat
     3481 aagtcggggc aatcgtacaa atattgtaca acctgcggac gttccagcgc gtgccggtgt
     3541 cgggcggctc ctttttgcag gggtaccagg taccagcccg gaggcaataa aaaagccgcc
     3601 ttgttagggc ggctctggtt acatggtgct gattggatcg ggccgattaa gcgaagtccg
     3661 cgccggttag ttgctcccct tcgggctggg attcgtccac gcctgggaag gttagcgcca
     3721 ggctcaagcc gttacgcttg cagaatgcgc ggcgcatctc gtcgaagtca gggcaataga
     3781 atacgcggct acctttgcgc cccgtggctt ctacgccgca atccttaaac gctgtcgcca
     3841 gttccatgcg gacgctggcg cgtgtccata cccctttcgt tctgtccttc gggttgttct
     3901 gcgttttgta gtccgcataa agctggtatg cggtttcgca cagcacgcca ccagcggaca
     3961 aaccaacgtt agccgtaggg aaatgcgctg cggcggctac ggtttccggc gttaccttgc
     4021 cccattgctc cgcgtcggcg cgacactcga acggtaccgg cttactcagt tcctccagca
     4081 taaaggtgtt tacttcgttc aggctggaaa gcatcgcatc tttaagcgca tcagtgtgcg
     4141 gcgcttcgaa cgggttaaac gcggaaatat cacgttccat aagccagccc gccaccttct
     4201 cgatgttgcc cggcgtcatt aaccacgcgt catatttggc gaagtaggcg gtattctgtt
     4261 tcacaatgtc cgataccatc agcataaacc aacggcgctc cgactcggtg acgcgtaccg
     4321 catcgcggcg gttactggcg gcaaccagac gggcgaagtt gtttatctcg atggcgtcca
     4381 cgcctttacg ctccaggcgc atacgccccg aagtgatgaa cttcttacaa ccatcctcct
     4441 gggcgcgggt agtaatgcgg gtttcatcag cgaagcataa caatttgcgc tcaatgtccg
     4501 cgttaaactt cccaattaac ggctctacgc cctgcgtttc aaggaagtaa gcgccgaaga
     4561 tgtactctag cgggcgcata aactgccctt tgcctacgcc cttttcgcca accaggacaa
     4621 ccgcatagcc tggtttttcg tccggcttct gtaaggcgtg ggcgaagaag tccatcagca
     4681 cggtgtaatg ctccgcgtta cctgcgcaaa taacatcgcg tgccaggttc aggaacggcg
     4741 ctatctcggc atcagatacc ccacgggcgg gcactacatc ccagcccaaa aacaggttaa
     4801 gcatgttgtc ggggcaacgc ttcggattcg ggaaaaagtc cattccgcca ggcttaaagg
     4861 aatgatccgg ccacgttaac cacgcctcgg caatgttcag cgcacgccct ggcttcttca
     4921 cttcttcgcc gtctttattg aacgtgacgg taaagccgtt aatcgctggt acatgctcga
     4981 aagcgccctt taactcctgc tttagtattg ggttaaagtt ggtctgccat ttgttattaa
     5041 ccaggcgttt gctggtggag ataacaaggt ttttgccccc tgcgataacg tgcgtatacg
     5101 ccttattcat attcgccagg cgcatgactt catccagttc cagcccacgg gctttaactc
     5161 cgctcaattc tgcgcgtagc tttttgccct tcaccggctg cgcggcgtcg tcgctatttt
     5221 cgggggtacc ctgctggggt gcctcgtcgg aaaatacatc cagcgcgggg gtgctcaagt
     5281 cctcgaaaat atctggatca gcacggttaa ggcggcgcgg ctgcttgaat ccatcgcgct
     5341 tacagttagc cagcacgcgc aaaatctcgc catcttccag cccggtagcc tctgccgttt
     5401 gagttaacag cgtttcggcg tcggcttcgg caatctcgcc accagcaacg agctggaaga
     5461 tagggaaagc gacggcggtt agcgtgctgt tgcgccctgc gtcgcagtct gccagatact
     5521 tgcgacaagc tgcttccagt gcgcggcgtc cgtagttggt ggagttttcg cacggtgcta
     5581 attcgcactc gacgcggaca acattcaccg gcttgcgcat cgccgctaac agggattcag
     5641 gcgctaccgg tagttgtgta cgggctggga aggtaccgcc gaacggcaac gccttgcccg
     5701 ctttgatgta caccggctga ttaccgcgct tgatgtccac gcccacggct aggcgtccgt
     5761 cctggctctg cttgtattcc tctggcacaa agtcaggcgg caacgaaaac agccagtgtt
     5821 tagagttgtt ggactcgcgc acctggtgcg gggtgcctaa gtccacgcca acaagctggg
     5881 ctaattccgg ctcgctgata actttcttct cgtcggcttt gttgccgtcg tagtccagca
     5941 aaaaggcatc atccaggata acggcgaaaa tatcctttaa cgtgtccggc gcgttccagc
     6001 ccggatgttt tggggtgtaa acctgccctt tcgcgtagcc gtaacgcgct gccggtagtc
     6061 catcagtcgg gttaatccag cacggtacaa tgcgccagcc attcgcgtac atcttcgcgc
     6121 tggtttccag tagccaggca ttcgggctgg cattctctgc gggttcgatt ccgtccatat
     6181 ttttggcatc cattctgtta agcgtccgcg ctggtggtgg tttcggtagc cggtgccagc
     6241 ccgtcagggg taacaaaggt ggctacgagc tgggcgatag tggtttctgg tgcgcggtat
     6301 gcgtcgccgt cgctcagtgc agggagtacc agcgaagcaa aagcgtgcgc gtcggctggt
     6361 gcctggcgtc cggtttcgcg gtagaagtcg caagtcgctt tccctttgtt aaccagctta
     6421 tgcgcgttaa ctaatagctc ttgctgctgg cgctgtacct tgcgcccgtt gtcattcatg
     6481 aagcggaaaa tctcgacgcg ctgcatacgg tagatagcga cgcggcccgc attcttaatg
     6541 cgcacggttt cgacgggtac cagccaacgc tgtttgatgt ggtcaatcgc ggtgcaacaa
     6601 tcctcgcggg ctggcgtcag ttcgtgtact tcggcgcggg ttacttcctt gcctaacagc
     6661 atttccacca gcgcggcgcg ggcaatcttg gcgcgtttgc tggttttgat ggcttcccca
     6721 aattccgcat cgatacgctt ttcaacctgg gcgcgggtgt ataacggttg tggtttacgt
     6781 gccatagcct tacgcctcct gcgccagttc agcgaagtga cgcgcacggg cgcgaagggc
     6841 tgcggcttct gcctctgcct tatctgccag cccggagaaa tagcgggcgg cgtaggcgtg
     6901 catatcgcat gcgtgatagg ttccgcgacg gtagccaggc acggacggcg ggaagttttg
     6961 cgcgttacgg cgatgttcaa agtacgccat gcgggatagc tccagcattt cggctgcggc
     7021 tggggtgtcg attactgtcg ggtttttcat gtatggctac tccattaact tggattagcc
     7081 aggcgggagg gtactaagtt gagtttttgg ctggcgtgca aaccgccgtt attgcacaaa
     7141 tactgttcaa ttaagtatcg tttcggatac tgcgccttgc cagcccacac tattacgggc
     7201 taatatcagc aagacaaaac aggccatgtg ccaaaaaaac ctcgctcggt cagagcaaaa
     7261 atcttagtag ttccgccgcc tggcgggtgt agccggtgaa attgctgatt cagatagata
     7321 gcttggcggt taacgtatct gaataaagcg caccggctgt ttgtttctag ccgttcatcc
     7381 gatttaaatc actgaataaa cagctatata ctgcaatacg cacaatagca ctttttaaca
     7441 attctgcata tccccgcccg tatcgtttgc ctatttctta ggcggtagcc ccgtgcctta
     7501 tcgttttttc gtccagcaat gtgcgccagc gcccatagaa ttgactgaat gctatcaatg
     7561 cggggcaatg tacccctgca aattacatcc cgttagaaac gcttacaggc gctttcctgg
     7621 ggtgcgctga atactttgta ttcggttgta ttcggtgggg tgtattcggt tgtattcgaa
     7681 tgtattcggt agggtgccag cccgttggct tcgggttggg ataggatcgg gagtatgcgg
     7741 gccgatactt ggactatgaa aaattgcgcg tatcttgctg ggcttctaag agagaaagcg
     7801 cggcggcgaa ttttgggggt acccctgccc ttgcccttgc ccttcagaaa atagaaaggg
     7861 gggtaccccc ccttcgttat tatcctgctg cccttcgcct tgtgtctggg ctgctcactg
     7921 cttacggctg cggcgctgct gctccttctt ccactgtacg ccaacggctt gcgcatccca
     7981 cgcaatgccg gtgcctcgct tgaatccctc ggcgttcagt gctgcggcgc aatcaacatg
     8041 actcgcgccc atatccttta ggctaatgag gcgtgccagt gctgccggtg gcgtgcctcc
     8101 cttcggtggc ttggggtatg ccgctaccgc atcgggctgg ctatcgggtg ccggtgctgc
     8161 tggtgggctg attggatcgg atgccagccc gttggcttcg ggtgtctctg gctcgatgcc
     8221 accagcggaa agcgaagcgc caatgtgcgc ggctagtgct tccagttgac ggcgcaaggc
     8281 gtctacctgc tgttgtatat cgtgcggcgc tactggctgg gcgctcggtg ctgctggctg
     8341 ctggcggcgt gcgtattcgg ctgcgataag ctgccggata aagttgctgg cgctgttgcc
     8401 gtccagcata gccagcatgt tggcgtgcgt cgtatcgtcc aggcgcacgg ttgtaatatg
     8461 ggttttgctc actcggtaga ttctccgggc tgggtgtatt cgtttgtatt cgttcttatg
     8521 gcggtggctt ggctgcgtta tcctgcgcct gtcgcggagg gttggcaagt tttgggatga
     8581 tgccagcccg ttggggtgtc gatggcgcgc atggttgggt gctgtttcgc tgcgcaccgg
     8641 cataaaaaag catgctttct gcgcgaacca cggcgcgaac cggtggcgct acgttaattc
     8701 tgtagggtaa tgggtgccgc gttacggaag ttttcacgta gcgcaccggc tgtttgactg
     8761 aatacagtca aagaatgggt aatgtttgcg tgtagcctgg cgggtattca gtgccggaac
     8821 gtgcggaaaa atcccctccc ctggggtgtc tcgataggtt gggggtacaa aaaagccagc
     8881 tcgatggctg gcttctgtta gctggtgggc tggctgttag tgcttagtgc gccgggtgat
     8941 gttgcctaca gtcgcgccca tatcgtcgcc gtacttctct ttcaggctgc gcataatctc
     9001 tgcggcttcc tcctgcgcac ggcgtaacgt agcatcatgc tcggattctt cttcgtcgtc
     9061 gtcattggct gcggctgctg gcttatcgcc attcatctgc gcgataaggg tatgaatgtt
     9121 ctttaccagc ccccacgttg cggcggcgct tttcttatcc caaagtctgt cgcctcgctc
     9181 ccctttggtt agctcctgcg tcggcttgtc gccaccagcc caatggcttg gatcggattc
     9241 ttccattgcc acggttaaca ggttatcggc aagcgcctgt aatgctgcga tgttgct
//
