LOCUS       CP134930                4715 bp    DNA     circular BCT 27-SEP-2023
DEFINITION  Escherichia coli strain 284 plasmid pMB6980_7, complete sequence.
ACCESSION   CP134930
VERSION     CP134930.1
DBLINK      BioProject: PRJNA924946
            BioSample: SAMN32977818
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Shropshire,W.C., Strope,B., Selvaraj Anand,S., Bremer,J.,
            McDaneld,P., Bhatti,M., Flores,A.R., Kalia,A. and Shelburne,S.A.
  TITLE     Temporal Dynamics of Genetically Heterogeneous Extended-Spectrum
            Cephalosporin Resistant Escherichia coli Bloodstream Infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Shropshire,W.C., Strope,B., Selvaraj Anand,S., Bremer,J.,
            McDaneld,P., Bhatti,M., Flores,A.R., Kalia,A. and Shelburne,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2023) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TEXAS 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/21/2023 12:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,805
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,453
            CDSs (with protein)               :: 4,453
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 68 of 231
            Pseudo Genes (incomplete)         :: 168 of 231
            Pseudo Genes (internal stop)      :: 35 of 231
            Pseudo Genes (multiple problems)  :: 40 of 231
            Pseudo Genes (short protein)      :: 4 of 231
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="284"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB6980_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-11-04"
                     /collected_by="MDACC"
                     /note="genotype: ST10"
     gene            complement(202..315)
                     /locus_tag="RNP63_24005"
     CDS             complement(202..315)
                     /locus_tag="RNP63_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WNL98330.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(969..1163)
                     /locus_tag="RNP63_24010"
     CDS             complement(969..1163)
                     /locus_tag="RNP63_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WNL98331.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2239..3195
                     /locus_tag="RNP63_24015"
     CDS             2239..3195
                     /locus_tag="RNP63_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL98332.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            3179..4045
                     /locus_tag="RNP63_24020"
     CDS             3179..4045
                     /locus_tag="RNP63_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WNL98328.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            4042..4695
                     /locus_tag="RNP63_24025"
     CDS             4042..4695
                     /locus_tag="RNP63_24025"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004722 - protein serine/threonine phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WNL98329.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="PP2C family serine/threonine-protein phosphatase"
     Typing          814..896
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          1200..1298
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          1423..1678
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          complement(244..801)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.821"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctattgagat cttcgtgtca tatagataat ctcttttctg taaaagaaaa aaaccgcctt
       61 tgcggggcgg ttttctctta ggttcaaaga gtttgcggct cgaagaacca aggtaactgg
      121 cttggaggag cgcagtcacc aaaacctgtt ctttcagttt agccttaact ggcgcatgac
      181 ttcaagacta actcctctaa atcagttacc agtggctgct gccagtggcg ttaaggcgtg
      241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcagt cgggctgaac
      301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
      361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      421 ggtaagcggc agggtcggaa caggagagcg cacgagggag ccaccagggg gaaacgtctg
      481 gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc
      541 tcgtcagggg ggcggagcct atggaaaaac gcctgcggcg cggccttctt cctgttcttg
      601 gtcttttgct cacatgttct ttccggcctt atcccctgat tctgtggata accgtgctgc
      661 cgtttttgtg taagtcagta ccgctcgccg cagccgaacg accgagcgta gcgagtcagt
      721 gagcgaggaa gcggaaaagc gcctggacgc gcattttctc cttacgcatc tgtgcggaat
      781 ttcacacccg gcatggggca cttttcatac aatctgcact gatgccgcat agttaagcca
      841 gtatacactc cgctatcgct acgtgactgg gtcagggctt cgccccgaca cccgctgacg
      901 cgccctggcg ggcttgtctg ctgccgaaaa cgctcgcgga caagccgtga gcgcctccgt
      961 cagcttcgtt atgttcctgt ttcactttcg gtctcaaagc gaacctggat gctgttctgg
     1021 agttcttccg cgagttcgtg cagtcgttcg cacatggccg cctgttcgtc ggcatccaga
     1081 gcatccagtt tttcaagcag tgtcaggctc tggcttttga taaatcgcgc catattcagc
     1141 gcagtttgtt gctgtttgtt caccttccgt tgttctccgt cagtaccggg tgtcgggtgg
     1201 accctgacca ggtggtaatt gtaagacgtt gcgggcgacg gtattacaat tacacatcct
     1261 gtcctgtttc tttaggacgt atcatggcac agcatatcaa ctgataaata atccctgtga
     1321 agtgcagccg ctgttgcata aaatttcgct ctgcgtcgag tgtttcttaa tcaaaaattc
     1381 cgctgatact ccggaaaaac gcgagtctgt tttcatgcct gaaatgccca caccccacgc
     1441 aaaaacaatt ttttgctgat ttttctttat aaatagagag ttatgacaaa ttagttcttc
     1501 ttgctctctt tgtgatattt aaaaaagcgg tgtcggcgcg gtgttgtagc tgcgccaaca
     1561 ccgcttttta ggggtggtac tgactatttt cataaaaaac atcactttat attaggggtg
     1621 ctgctagcgg cgcggtgtgt ttttttacag gacgcccctg ggggcgctgc taggggtgtc
     1681 tgttcagata tgtagcaatg agtcagggat tcacgaactg aaaatgtccg aaaatgtgcc
     1741 ttatgggcac cgtatgtaac caaaccgatc gccattcact ttcatgcata gctatgcagt
     1801 gagctgaaag cgatcctgac gcatttttcc ggtttttact gggtaaaaca tctctttttg
     1861 cggtatctgc gtcagaatcg cgttcagcgc gttttagcgg tgcgcgtaat gcgacgttat
     1921 ggtaaatgac gctgaaaaat gatcttttac gctcatcgtt tatgagcaac agatgtccta
     1981 atcatggatt tgaactataa ccgccgctga attgatcgca tgaggtgtta tgaaaacggt
     2041 gatataagta aactttttct tgacttccta cttattatcc tataaaataa atccttagga
     2101 tttattttat aggatatcaa aaatataaaa atggcaaatg cttgtttgtt attatttttt
     2161 tcttgacaat caatctctaa tagaatatac gtccttagga cgtatattct attagagact
     2221 aaaccgaaaa ggttgaatat gtatcttatc gaagaagcgc taacgtcttg gttgttgaat
     2281 gaatataaaa aaccagtcat aaccaaagaa gaaatctctt tgcagattta ccttttttat
     2341 ttaaaaaaag aatataatgg tgagaggata tatcaaatta gaaagaacaa agcaggacct
     2401 aatgaatttg aaaaaaatat attaaaatta ttaaagtcag gggttttatc aagagttgaa
     2461 agtaacgata agcatcatgc ttacccccat ttaattggcg gtttctatga ggtctttttt
     2521 gttaaaggta acgatggttc tccggaaaat gatatagtgt gctctacgta tccatatgga
     2581 tacttatcct atttatccgc tatggattgg cataacatca cagataaaat acccaagata
     2641 atccacttta cgacatgtcg ttccgaacag tggaagaata agttctatca tgattttcaa
     2701 aaggataatc ctgatataga cgaagccaaa caattcatgc ctagatttcc aagaaattct
     2761 gaggtcaatg gaataaaaat tatcgttaca caagaaacaa attatgttga gccaatgaag
     2821 gctcgtaatt cccctgtcag ggtatcatct atcggtaaga cctttatgga tatgaccagg
     2881 cggcctgatt actgtggtgg tgaggagcat gtattggaat catttattga gcacggtaaa
     2941 aaatatgcac ctctgattat aaaggaagtc gataagcttg gccgtcatat tgacaaggta
     3001 agggttggat tcattttgga tagaattatt ggggttaata ataaaaaaat aagcgaatgg
     3061 aaacaagagg cgggtaaata cagaggtagt tcgaaagtgc tatctccaaa gagtccattc
     3121 tcaccttact ttgatgaaga atggtttatt tctattaatg ttgatatggt taagtcttat
     3181 ggaaaaagaa attaataatt ggatcgaaga gtctggtggg aagtacaggt tgatgcggca
     3241 ggctgtacat ataatactta aatctatttc tatagaggag agtttgaatg aaatgatggt
     3301 tatgaaggga gggactcttc ttggtgttaa gcatggtagt gatagataca caaaggatat
     3361 tgatttttct accgaaaaaa aactttctga aattgacttg ggggattttt tcaataggtt
     3421 taatgactca atggatgttg ctgaggctga attgggatat ggtattaaat gtaaagttca
     3481 atctcataaa attcaaccta atgcacaagg aacatttcca actgtacaga taaatatagc
     3541 cttttgcgat cgttctaatg cgtctgcaat gaaaaggctt gaatctaatc aatcaccttc
     3601 agtgataaat atcgatttta gctttaacga aaaaacgtat gattttgact ttttatcatt
     3661 agatggtgat gatgacaatg atagcgtaaa gacctacagc ttaactgatt tgatggctga
     3721 aaaatatcga tccgttttgc aacaaaaagt tagagagaga aatcgagagc aagatatata
     3781 tgatatcaat tatcttttag gtaaatatga gttttctcgg caagataagt ataaaatact
     3841 ggagtctctt ttaaaaaaga gtgaaggtaa acaattggat aatctcttaa acgttaaagg
     3901 tataagggat gtagaaatta tcgagcgttc cagtcagcga tatcaggatt tatcatccac
     3961 cgttaaatca ttacctcttt ttgaagatgc ttatgaaaaa gttgcctgtt tctatgaaag
     4021 tcttccgtgg gatatattca aatgatagcg ttcagtttta gtgatattgg taacagatgt
     4081 gaaaacttag acttattatc tacgataaat tgtcaaaatg atgtgtttgg aatggttatt
     4141 atggatgggt attcctgctc tgagtggaat atgaaaattc tctctggtag gttagataag
     4201 tgcgtgtttt ctggtgatct gaataaagat attattgaac catgcaagtc agttcctgtt
     4261 aaggctgcag tatgtttcct ttttaaaaat aatacgcacc tccatatata taacgcagga
     4321 gattgccgtg tatataacaa aagtggagtc cttctaacag aggataactc ggttgcctgg
     4381 aaaagattaa aggaaaatgg aatcgataat aaaaaaatac ctcacctcgt ttgttattgc
     4441 ccagacagaa attatctaac atcatttgtt gatttctgct ctgatgagat aattttcaat
     4501 cattcagttt ttgatttaaa tgatgaaaac aaagaggtta ttttatgtac tgatgggttt
     4561 tgggagcatt ttaatgatgg tactgttatt gacctcatga aagataaccc agaatctaac
     4621 ttggctaaat ttactaaaaa tatcccgggt aacgcagaga atcatacggt ttgctacgtt
     4681 agattccctt cttgatttcg tgttttttga agaag
//
