LOCUS       CP134895               13546 bp    DNA     circular BCT 27-SEP-2023
DEFINITION  Escherichia coli strain 4080 plasmid pMB4926_4, complete sequence.
ACCESSION   CP134895
VERSION     CP134895.1
DBLINK      BioProject: PRJNA924946
            BioSample: SAMN32977769
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Shropshire,W.C., Strope,B., Selvaraj Anand,S., Bremer,J.,
            McDaneld,P., Bhatti,M., Flores,A.R., Kalia,A. and Shelburne,S.A.
  TITLE     Temporal Dynamics of Genetically Heterogeneous Extended-Spectrum
            Cephalosporin Resistant Escherichia coli Bloodstream Infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Shropshire,W.C., Strope,B., Selvaraj Anand,S., Bremer,J.,
            McDaneld,P., Bhatti,M., Flores,A.R., Kalia,A. and Shelburne,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2023) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TEXAS 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/21/2023 12:23:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,726
            Genes (coding)                    :: 4,605
            CDSs (with protein)               :: 4,605
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 47 of 121
            Pseudo Genes (incomplete)         :: 65 of 121
            Pseudo Genes (internal stop)      :: 29 of 121
            Pseudo Genes (multiple problems)  :: 21 of 121
            Pseudo Genes (short protein)      :: 2 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4080"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB4926_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-01-03"
                     /collected_by="MDACC"
                     /note="genotype: ST196"
     gene            complement(join(13291..13546,1..233))
                     /locus_tag="RNQ29_24105"
     CDS             complement(join(13291..13546,1..233))
                     /locus_tag="RNQ29_24105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70642.1"
                     /translation="MFKSDLSDSQFEKLVALQKYLPSDDQPTRQAKHLLVEGFTPEFVA
                     EYTGVKLEKVQKLYEESWNPRCRVPLSVYNEIDRDRNVFEDRRRKSLLMWNCGMNIEEI
                     AKELKVTCFHIWGLLSEILPRKELNQRLPKPGSRAYREFIFIYRRHRNKVQKAMAQKA"
                     /name="hypothetical protein"
     gene            complement(569..1486)
                     /locus_tag="RNQ29_24110"
     CDS             complement(569..1486)
                     /locus_tag="RNQ29_24110"
                     /inference="COORDINATES: protein motif:HMM:NF025107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WNL70643.1"
                     /translation="MSNNEYGELFTPSFKRMLQLEFKKPVATTVNKNTGEVKHRYKLNT
                     NEMLVYGEIACFHFHGKTVYISEQTLADQYGWTPKTVRTAIHSLENAGLINVKREQGKS
                     NIYELLVTPSQIGEMCDQQGVELFKGYKAAAEQVKQVDNQSQEAPKQATQQAKKAEVVQ
                     LPTQDTKAPQINTVSDKPRRGRPVKDVTDGGWIVLGESEAKDRMRSLWESKYKGLNKRC
                     PIRLSKIQEDFYTTEEVMRHKGNRDGEIWKPEEYWHLTRPVFIFANKLIGMGFEILLDQ
                     TYGDYPEILWRNVRKNKKGILEFR"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1479..1706)
                     /locus_tag="RNQ29_24115"
     CDS             complement(1479..1706)
                     /locus_tag="RNQ29_24115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70644.1"
                     /translation="MAGYESPVLSWSVDEIVEKVMSGKYRLPSLYAVNKRTGKIHPHIE
                     QAIKQIRILKLQEKLKALEAERDTGRGKNE"
                     /name="hypothetical protein"
     gene            complement(1846..1995)
                     /locus_tag="RNQ29_24120"
     CDS             complement(1846..1995)
                     /locus_tag="RNQ29_24120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70645.1"
                     /translation="MAISDAIAIINLAVLWCGVAYIAGVTAIDNLEEKMIDKYKKIVDN
                     LTNA"
                     /name="hypothetical protein"
     gene            complement(4394..4981)
                     /locus_tag="RNQ29_24125"
     CDS             complement(4394..4981)
                     /locus_tag="RNQ29_24125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70646.1"
                     /translation="MNIKTIIAAITLAFSANTAAIDVGAVKDASCADKLAALNAGFDAP
                     QGYQMGLSLKNIILPMESGAYVYGAIGKLEKPEIDLQGVRAKITKRNESLCDVAEIAFM
                     SDDEKSILAFCKDSDDNDRFFKITHNRPISFYSNATMGAGMQWLKRAKLTEIDIEKEAN
                     AGSYGFWKGWTKYTFQQCGIDSQAYDNGFWFF"
                     /name="hypothetical protein"
     gene            complement(5029..5292)
                     /locus_tag="RNQ29_24130"
     CDS             complement(5029..5292)
                     /locus_tag="RNQ29_24130"
                     /inference="COORDINATES: protein motif:HMM:NF014814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WNL70647.1"
                     /translation="MTGAKDIKRLREELISEVKAKAKQMEIAAQMDVAEQMYRMVEEGT
                     PRQVIAKTFGVGIATVHRRLKTYADYHNIERKEDGRFKKNKQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            6325..6660
                     /locus_tag="RNQ29_24135"
     CDS             6325..6660
                     /locus_tag="RNQ29_24135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70648.1"
                     /translation="MKDMTIRTRGHIISYAVDEQFAIRDVEVKNKVKAEIPAGTVVVVT
                     EAGEVETFVKATHTDKTPLLVVEHAEVGDAFIVVAHKGCYINPAYVDKDGLEAVIAASG
                     GDLRVAQ"
                     /name="hypothetical protein"
     gene            6672..7715
                     /locus_tag="RNQ29_24140"
     CDS             6672..7715
                     /locus_tag="RNQ29_24140"
                     /inference="COORDINATES: protein motif:HMM:NF015802.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="WNL70649.1"
                     /translation="MAKKEFEVRDLCGLIKDIPLDNQIISAMNIFNTTSLCNTFKFEYD
                     KVRQVKQTAFGQYPRYGEQMATITADERETGTGDMAFTAASSAIFPSDWQGIRVPGKDS
                     EENLESVTARRIVQLGEGLRQTHEEARQLALFENKIILTKGKELDVCDLLGVEQTTSEV
                     DLAKDILKQLQDLVALSKVKQRGVVRALRGFTLFCRAELFAAIQTDELLRKVLLFGNQN
                     ITDKGLLFPEDILPGFPTFRIPGLALRVVQMDNLPENTAYLVPQFQPYALGDMGVYQQF
                     FGPCARNLKLANGAARDAFMYMEESRGKREIFVESSYAFLNMMPEVVVKASVSNSAAAA
                     LAKAKAK"
                     /name="major capsid protein"
     gene            7830..8081
                     /locus_tag="RNQ29_24145"
     CDS             7830..8081
                     /locus_tag="RNQ29_24145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70635.1"
                     /translation="MKIVLYGDRDDVPFDIIHIDDEDLPEFLRISTFAACYDVSRQAMH
                     KYREYNKCTWAETFEHFIARKQAQDRKKALNAHNRFAK"
                     /name="hypothetical protein"
     gene            8156..8959
                     /locus_tag="RNQ29_24150"
     CDS             8156..8959
                     /locus_tag="RNQ29_24150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70636.1"
                     /translation="MASDALTAAKQAESRSPAFTVMCGGYSQNKNIMDGFNYTDNVAIF
                     FDSISDVSYSFPVEKSSYAVEDGTTMSDHAVIKDKSVSFTGIITSSPYIIRKENYIDRN
                     TDPDNPRESMRPAAAVQALMEMRDKRQLVSLVSEDAGLLENFIMTKCEVKRSSSSGDCI
                     TIDCTFEEFRTFKVKMVENAGVFTNSKKVNKTQQKGAVNSAKEGSNAGNAATKSTRAKH
                     RGEYAQGEAAGKIAAGDKSVVSNEFHETMIDKANMLLDDITEINK"
                     /name="hypothetical protein"
     gene            8956..9414
                     /locus_tag="RNQ29_24155"
     CDS             8956..9414
                     /locus_tag="RNQ29_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016068527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70637.1"
                     /translation="MSPKVRCTIDKRMMDKLHKELSQMEGVAVGWDDGKHYSGLNYATL
                     AAIHSEGWGDLPKRSFMESAHAAMLYESLRHTKALINAIVKGGSSEAVRKRIGKRYADI
                     LETILISGQFSNNKVSPQWASYKGFNDAMRHYDDLIGAIKYWSNVGRG"
                     /name="hypothetical protein"
     gene            complement(9965..12085)
                     /locus_tag="RNQ29_24160"
     CDS             complement(9965..12085)
                     /locus_tag="RNQ29_24160"
                     /inference="COORDINATES: protein motif:HMM:NF040129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="WNL70638.1"
                     /translation="MAVKEVARLTNVIYWDSDKSSWRKEVKRLEDYKKSWKKATDEVAK
                     ADFKSNQAVLRTQRTRAKVEEQIARAQRKQQEAQKKQRAQAVSRLTGGNNPELASMRKF
                     YQQQAREAARQQKLLERSVGDTQHRKARVGANSARISGMFDGSIVSQFQGRIQRVNADY
                     AKGAINLQTYNQRMARLRSEMKMATAEAGKFQSRLDRAAVAGSKLHDNLGKLNSTLGKI
                     AIGLTAAGWGVQRAVSGAMSSLADRNSAYFGLMAQNKDDKAKTDQQMAFIKNTANAYGM
                     DQREMEGGYMRFAASTPTMKESDKQALFEAMAIKGRSVGATGEQQNRALVAIQQMASKG
                     KVQAEELRGQLSEAFAGSQQDFQQAAIDAGIIKNAQEFDKAMKDGKITLDALLPSLVKI
                     WGAAKETKAFAESMKQPEVALQRMKNSFNELAIQFMGVVDPADGVISLSESLISIFEDL
                     TTDMDGSGSAAKQFGEAVGAAMREIMYWVGFAKGYIEVKAKEFGMTGSEVGELVGKLVV
                     GLAGLKVITTLLSPLTGFVSLVTQLIALLGKIPGMGKLMSSAPSGAAAGAAAISAGSNA
                     ERSLIDGKMQEMSKENPKQGALFELGRFVSNAGALFTPGGDTGPLFDQVKGWFNKGPTL
                     AVDTGIRPTPSTGMAIQNGGYQPTQISVVPQGEISGKWEITIDEGGIARVIQKEVSDQL
                     DWKATQEANQLF"
                     /name="tape measure protein"
     gene            complement(12087..12335)
                     /locus_tag="RNQ29_24165"
     CDS             complement(12087..12335)
                     /locus_tag="RNQ29_24165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70639.1"
                     /translation="MIMNTYEKIYQKLVEGSEEGSSIAFGEGLAEQLNPQRGPTIIKSG
                     SHTEGLSIEELADKYNIDKEALERAILLSKSLKQGEV"
                     /name="hypothetical protein"
     gene            complement(12332..12715)
                     /locus_tag="RNQ29_24170"
     CDS             complement(12332..12715)
                     /locus_tag="RNQ29_24170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70640.1"
                     /translation="MNIEEMIVEFEEGDFLEDGEFKYSLFKQALFSTDTKPHVSDLNDD
                     DLCRYICTRLYEEGSYTGQNFPDLYFKAQTFEEGLRVENLVDKMLSAGYLHTELLNPKA
                     ESKRHYIFVWSVGDKCPLRRKGK"
                     /name="hypothetical protein"
     gene            complement(12774..13217)
                     /locus_tag="RNQ29_24175"
     CDS             complement(12774..13217)
                     /locus_tag="RNQ29_24175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNL70641.1"
                     /translation="MSQIAKLLSQVIKAFDAEDSKVRQDLLDGKFTNTLAFTRDKEHFL
                     KHRHHAFAECKSANGDVFYVDLVVMACRLWMIQDLMRQHGDDPLISMWNSAQDGFVIAA
                     LSKSMIEAEGASLQNMADLFEISVGTMQTMLQAVKPLKLVNLD"
                     /name="hypothetical protein"
ORIGIN
        1 tctatttcgt tgtagacact gaggggaact cgacaccgtg gattccagga ttcttcatat
       61 agtttttgga ctttctcaag tttaacgcct gtgtattcag ccacgaattc aggggtaaat
      121 ccctcaacta ataaatgttt agcctggcgt gttggttgat catcactggg gagatatttc
      181 tgcagtgcta ccagtttttc aaattgggaa tcagaaagat cagatttgaa catgattaat
      241 tctccgaacg tttaaaagat cagggcaatc aatctagttc tagtgcttag tttatttagt
      301 ttgtttgggg ataacagagg gtactaatta ataagggatg caggagtctc tctcggttat
      361 ttccatccag ggatcaagct gcaactcctc gtactgttct tgcgtctctt tatcgataga
      421 aattaaatac tccatctctt cctcttcgat tatgtgatta tagtcataat cagaaaggtc
      481 gataccaggg attaaataat cgtttaatat gctcataata aaatctcaca aaatataaat
      541 ataaaaagaa gaggaattat cagtattttt atctaaactc taaaatgcct tttttattct
      601 ttctaacatt tctccacaat atttcaggat aatctccgta agtttgatct aacaatattt
      661 caaatcccat cccaataagt ttatttgcaa aaataaacac aggtctggtt aaatgccagt
      721 attcttccgg tttccagatt tcaccgtctc tattgccttt atggcgcatt acctcttctg
      781 tggtgtaaaa atcctcctga atctttgaca aacggatcgg gcaacgtttg tttaaacctt
      841 tgtatttgct ttcccacagt gagcgcattc tgtctttagc ttcgctctca cccaggacaa
      901 tccagcctcc atcagttaca tctttcacag ggcggccccg gcgtggttta tcgctaacag
      961 tgttaatttg aggcgcttta gtgtcttggg taggcaattg caccacctcg gcctttttag
     1021 cttgctgtgt ggcttgtttt ggggcttcct gcgactgatt atctacctgt ttcacttgtt
     1081 cggcagctgc cttgtatccc ttgaacaatt caacaccttg ctgatcgcac atctccccaa
     1141 tttgtgacgg cgtaacaagt aattcgtaga tatttgattt gccctgctct cgtttaacgt
     1201 ttatcagacc tgcattctcc agggagtgga ttgcagtcct cacagtttta ggcgtccatc
     1261 cgtactgatc tgccagagtc tgctctgaga tgtaaacggt tttaccgtgg aagtggaagc
     1321 aagcgatctc accgtaaaca agcatttcat tagtatttag tttgtagcgg tgttttactt
     1381 cccctgtgtt cttattaacc gttgttgcaa ctggtttctt aaactcaagt tgcagcatac
     1441 gtttgaaaga tggggtgaat aattccccat actcgttatt actcattttt cccccttccg
     1501 gtgtcgcgtt ctgcctctaa agctttcagc ttttcctgta atttcaatat tctgatctgt
     1561 ttaattgctt gctcgatatg tgggtgaatc tttccggtgc gcttattcac agcgtaaagg
     1621 cttggaagtc tgtattttcc agacattacc ttttccacaa tttcatccac agaccaacta
     1681 agaacgggcg attcgtatcc agccataatc aaagactctt ttagtagacc ttgtgacaaa
     1741 aaagaagctg ggatgtaacc cagcatgaga agtttttatg agcacactaa gcattatagc
     1801 tatcagtatg cctaagcagg atttctccca ctgttatata ttttatcagg cattcgtcaa
     1861 attgtcaaca atctttttgt atttatctat cattttttct tccagattgt cgattgcagt
     1921 tactccggct atgtaggcaa caccacacca cagcacggcc aggttgatga tcgctattgc
     1981 atcactgatc gccatattga cctccagatc tgtttcttca ccatgtatat aatcttatca
     2041 tactgtcaac agaatgtcaa taggaatcat tatcatttag gctttctata ggtgcaagca
     2101 agtccacagc aaagggggta agcattatta atggccccta ccgtgtcaaa actataatcc
     2161 cttatagcat ttatgcagta agattgtcaa gccataaatg gggcgggtgt aggcatttaa
     2221 ctttaagccc tctgagaggc cctgtaacgc gatttaagca tcaggcaatg caattcacta
     2281 ccccacacag ttaatgcagc cagaatgcca tttaggggcc ttgtagcccg tttaaactcg
     2341 tgcaagtagc actcatcccg agagcgttaa actcgtgcac acgcgcggga gactcctacg
     2401 cttaaacggt ccggattgga aacagaagtc tcttatccgg actccacatt atacagctac
     2461 gtgcttcgca ctacgctccc cacacaggcc gacgtagtcg gttaagctgg atagcttaag
     2521 tgctggaagc accccacaca tgtaaacagg gtgacgtgtg taatgtatgg aatacattgt
     2581 cacccctaca catattgcct aaagaggatt agcaactctt taggcttctc tttgtgtggt
     2641 ccacttcgtg gatttagctt tcagctaaac ccttcgggcc tttgtggggt atcgactttt
     2701 catctctcta tgaaaactct caccacccat tccccctggg ggggttaggg gggatatgta
     2761 tttattgtct attttattta tctattagtc ggtaaatttt accgtggtta ccccggtaat
     2821 ttttccccag gtacctcggt aatttttacc tgggtgatgt acctaccccg gtaaattctt
     2881 ccctggtctg ctggtcagac tcaccttata cgcggcccgg aggtgggtaa attcttccgt
     2941 tgtcgttttc atggtgggta aatttggtgc ctctcatcgt gatggcatgg cttacacccc
     3001 ggtaaaattt accggggttg gagacaccgg agaaattctc agtagtagtg taggaaatac
     3061 cgacaagtgg gggattgatt gcaccggatt ttgactcaat gtgtcttcaa ttgactaaca
     3121 aacctctctt ctccctcttt gctctgattt cctctcatgt gtgggaaata tgcagatcaa
     3181 cacccgttga ttttatcaac acttatgttg accgggtatt gtaatagcat cataactatg
     3241 gcccctggag cagtccatcc ttccctcaca caggccaatg aatatataaa gaacatgaaa
     3301 gactgttcac accaacacgc cgataaattg cctgaagagg cttacatggg gttattgttg
     3361 tgtgggtaat agtgatcatg ctaacagatt atgttaatca gatgtaaatt ccgattttgt
     3421 ggtctgcaga taacaaacta tactggttaa atgatcagga gaatgactac aaaacaatca
     3481 aaaatgtggg atatagccat ctatacttct aatgtgctgt gtggggtgtt agtgcatact
     3541 tacatgtaag tcattgattt gaagggttgg gaaaattgca tttaacataa tagatcttat
     3601 ctacactgat aaagcgcact acaaaatctg atttaaaaat catgatcagt gggcacagaa
     3661 tttacgtaca cgcacctgaa taaataatgt gcacaacata cagcaaagct taagaattaa
     3721 catagtagca acgcggtcag tagtggtggg atatttgaga attgttagaa gaaaaaaggg
     3781 aggtggggga gcgcctgcct ggcacctcag acgagtctcc gatggatatt aataccacta
     3841 atggatattt ctgacaccca cacctaagac cgctttcagc acatagccat acgccgcaca
     3901 ggatgcaatt gtgataacaa aaaatagggg tgtaataggg taggtcatct ttcattaaaa
     3961 tcatctctac ggcgtttcca gagtgataac aagaaaacag gcctctaaga ttatctgtgc
     4021 ctcgttttta gctttttgtg tggttaccag gtgaagagga aatgttaaaa actgttcatt
     4081 ttatgcacaa actagtcagg gtgataccac aaaatgaggt gcaagaaatc cagctgcaga
     4141 taatcttgca gccagatttt gcagtttttt agggtatgtt aaatttgaca aaattagaga
     4201 agaggctgat ttagctaagt tattgatttt actcattatc taaaaatggg tgatcacctt
     4261 actcttacac tgtcaacaat gcctaaaacc attaaatcaa taaataggca ttaaataccc
     4321 attaaatagg ggttaaatag gggataaata aaaaggggcc gtgatggccc cctggcggga
     4381 ttaacttagt aggttagaaa aaccaaaatc cgttgtcgta ggcctgacta tcgatgccgc
     4441 attgttggaa cgtgtattta gtccagcctt tccagaatcc ataactgcct gcattggcct
     4501 ctttttcaat gtcgatctca gttaatttag ctctcttcag ccactgcata cccgcgccca
     4561 tagtagcatt agagtagaaa gagattggcc tgttgtgggt gatcttaaag aagcgatcat
     4621 tatcatcgct atctttgcaa aaggctaaaa tagatttttc atcgtccgac atgaaagcta
     4681 tttcagctac atcacaaagg ctttcatttc ttttggtgat cttcgctctc acgccctgca
     4741 ggtcaatctc tggcttctcc agttttccta tcgctccgta tacataagct ccagattcca
     4801 tggggagaat aatattttta agagatagcc ccatttgata gccctggggt gcatcgaatc
     4861 ccgcattaag tgccgccagt ttatcagcac aagatgcatc ttttacggcc cctacatcaa
     4921 tagctgcggt gttggcacta aaggcaaggg tgatggctgc aatgatagtt ttaatgttca
     4981 tagtggtacc cctttgcgag aagttgaatt aagttagtaa atagcccttc actgcttatt
     5041 cttcttaaat ctcccatcct cctttctttc gatattgtgg tagtcagcat aggttttcag
     5101 ccttctgtgc acggtggcga tccccacacc aaatgtttta gcaataacct gtcgtggtgt
     5161 tccctcctca accatcctgt acatctgctc cgctacatcc atttgtgcgg caatctccat
     5221 ttgctttgcc ttcgctttta cttccgatat gagctcttct ctcagtcgtt ttatatcttt
     5281 agcccctgtc ataaactcct ctcaatgctg atcaatacaa aatataagac tgccagactt
     5341 attaaaatgc tttgcctcct ttgctcatcc tcacacctcc acacacagag ctaacatcag
     5401 ggctaagaca ccaaccactt caattgcctt aaccatgtgt gggtgatgaa acctggcctt
     5461 ttgccatgcg ctggcactgt tcttgatacc cataactatc accttttgcg agaagttgat
     5521 ttgtttgttc ggttgggttg ctatcaatgc tccccttcct tgattcgaac tatagccggg
     5581 taaatccccg gaaaacattg atattgatca aaagatatct atcacgaatg aaaaagttat
     5641 gatagatgcg atgagatgac gtattgatgg gattgataga ggtctgaaaa taccgttact
     5701 acgctaatta ttagaattta tataatcagg gtgttgacaa tgtgagtaaa tactgatata
     5761 atatacagta taccgtgaat catttagcga aggtgatttg ccgtattttc ctccaaagta
     5821 acggcgacca ggtagcggtg ccgccttata aataacacta ccctgatcat cgttgagtgg
     5881 tcgattaatt ctcctattgg gctagttatc cacaccgttt tcttttgcgg gttttctgtg
     5941 tgggtagcta tcccgaaagg tatttccccg ctgatgtgac cgcagcctga actgattcgg
     6001 gggagtgcct ttcgtgatag tgctctctag taaccgtaac catactgtga actatcatcc
     6061 gactgttgtc ggtttcccca tatggaatag cccccgcttg gacggtgcgg ggctatctcc
     6121 ctttcctagt atcaatctcc ctttcaatag atttccggcg tagccctttc ggcctgagcc
     6181 tgtggcttgt gggtgcttcc agcactttag cttacagcta aacccttcgt gcctgtggca
     6241 tgactacagg atggcatgat tcaaggggtt atcccggaac tctaatgaaa tgaggtgaga
     6301 tgcattttag tttcaggtga tcaaatgaaa gacatgacta tccgcacacg cgggcacatc
     6361 atttcttacg ctgtggatga gcaattcgct atccgtgacg tggaagtgaa aaataaagtt
     6421 aaggctgaga tcccggccgg tactgttgtt gtggtaactg aggctggcga ggtcgaaacc
     6481 ttcgttaaag caacacatac cgataagact cctctccttg tcgttgagca cgcagaagtc
     6541 ggtgatgctt ttatcgttgt tgcccacaaa ggctgttata tcaatccggc ctatgtggat
     6601 aaagacggct tagaagctgt tattgctgcc agtggtggcg atttacgtgt tgcacaataa
     6661 ggaggttgaa aatggctaag aaagaatttg aagttcgcga tctgtgtggt ctgattaaag
     6721 atatcccgct ggataaccag attatttcag caatgaatat cttcaacaca acttccctgt
     6781 gtaatacctt taaatttgaa tatgacaagg tgcgccaggt taagcaaacc gcttttgggc
     6841 agtatccgcg ctatggtgag caaatggcga ccattaccgc agatgaacgt gaaactggta
     6901 caggtgatat ggcatttact gctgcatcct cggctatttt cccttctgac tggcagggta
     6961 tccgtgttcc gggcaaagat tccgaagaga atttagagtc tgttaccgct cgtcgtatcg
     7021 tgcaacttgg tgaaggctta cgccagaccc acgaagaagc acgccagctt gctctctttg
     7081 agaacaaaat tatccttacc aagggtaaag aactcgatgt gtgtgacctg ctgggcgtag
     7141 aacaaactac ttccgaagtg gatttagcta aagacatcct taaacagctg caggacctgg
     7201 ttgcactgag caaggttaaa caacgtggtg tcgttcgcgc cctgcgtggt ttcaccctgt
     7261 tctgccgtgc tgaactcttt gctgcaattc agactgatga actgttgcgt aaagtgctgc
     7321 tattcggcaa tcagaatatc accgataaag gtctactgtt ccctgaggat attctgccgg
     7381 gcttcccaac cttccgcatt ccgggcttag ccctgcgtgt ggtccagatg gataatctgc
     7441 cagagaatac tgcttatctg gtcccacaat tccagcctta tgcactgggc gatatgggtg
     7501 tctatcagca attctttggc ccttgtgccc gtaacctgaa gctggcaaac ggcgctgcac
     7561 gtgatgcatt tatgtatatg gaagagtcca gaggtaaacg agaaatcttt gttgaatcct
     7621 cttatgcttt cttgaacatg atgcctgaag ttgtggttaa agcgagtgtt agtaactccg
     7681 ccgccgctgc tctggcaaaa gctaaggcta aataaggtcc ttcttcgaag gccaaataaa
     7741 agcaaaggga agaggtgaga gcaggctaaa taggtctttc cctcttccct tttttgttat
     7801 ccccgtctta agtttttgag gtgtttttta tgaaaattgt tttatacggc gaccgtgatg
     7861 acgtgccttt tgatattatc catatcgatg acgaggatct gccggaattc ctgcgtatct
     7921 ccacatttgc cgcctgctat gacgttagcc gccaggctat gcataaatac cgtgaatata
     7981 ataaatgcac ctgggccgag actttcgaac attttattgc acgcaaacaa gcccaggacc
     8041 gcaaaaaagc cctgaatgcg cataatcgtt ttgcaaaata acgatagata gccctgtctt
     8101 tttatcagaa ttcacgcagg gcaacgataa gttctatttt ggaggcatgt ttaatatggc
     8161 tagtgatgct ctgacagctg caaaacaagc tgagtcgaga tctcctgcat tcactgttat
     8221 gtgtggggga tattcacaaa acaaaaatat aatggatggc ttcaactata ccgataatgt
     8281 ggctatcttc tttgactcta tctctgatgt cagctattcg ttccctgttg agaaatcgag
     8341 ctatgcggtc gaggatggca ccaccatgtc agaccatgcc gtcattaaag ataaatctgt
     8401 cagctttaca gggattatta cctcttcccc ttatatcatc cggaaagaga actacatcga
     8461 ccggaacact gatccagata atccccgtga atctatgcgc cctgccgccg ctgttcaggc
     8521 acttatggaa atgagagaca agcgccagct ggttagcctg gtcagtgaag atgccggact
     8581 tcttgagaac ttcatcatga cgaaatgtga agttaagcgt agctcttcct ctggcgactg
     8641 catcactatt gattgcactt ttgaagaatt ccgcaccttc aaggtgaaga tggtcgaaaa
     8701 tgccggagtt ttcactaaca gtaagaaggt gaataaaaca cagcaaaagg gtgctgttaa
     8761 cagtgctaaa gagggtagta acgctggcaa tgctgctacg aaatcaacca gggctaagca
     8821 tcgcggggaa tatgcacagg gagaggcagc gggaaagatt gccgcaggtg ataagtctgt
     8881 tgtgtcgaat gagttccacg aaacgatgat tgataaggcc aacatgttgc tcgatgacat
     8941 cacggagatc aacaaatgag tccgaaagtc cgttgcacca tagacaagcg catgatggat
     9001 aagctgcata aagagctctc acagatggaa ggtgtagcgg ttggctggga tgacggaaag
     9061 cactatagcg gcctgaatta cgccacactg gcggctatcc actctgaggg gtggggtgat
     9121 ttgcctaagc gctcctttat ggaatctgcc cacgctgcaa tgctctatga gagcctgaga
     9181 cacacaaaag ccctgattaa tgcaatcgta aagggtggga gctctgaggc cgtcagaaag
     9241 agaattggca agcgatacgc cgacattttg gagactatcc tgattagtgg gcaattcagc
     9301 aataacaagg tttccccaca atgggcaagc tataaaggct tcaatgatgc aatgcgccac
     9361 tatgatgatt tgatcggtgc aatcaaatac tggtcaaatg ttgggagagg atgaaaaaag
     9421 acaatagtgt tcttttgagg ccgtagtgat acaccacaaa tcagcccctt tccacttctt
     9481 tctcgcgatt ggtcccttgt gtgaatcctt tatcaggtat gcattgtccc gtgcatgagc
     9541 ggtcgataga gcaagcctct ttcccggact tgttaggatt tagtgcgctg attgagcgcc
     9601 gctatcactg ttagcctgtt tatcgttgcc tggtttgtgc atcgctgcac cccctgggca
     9661 ccggacccgc ctcaaaggtc ggatcatcac aattggcggg gcgattttat agtcgcttta
     9721 aggtttatag accggagttg acttaccttc cggcaacctg ctaacattag tctcgcgaga
     9781 ggttgatttt tgttagcagg ttcgccccgc atcaaaagga taccggagaa gagccccttt
     9841 gttaacactt aggggctttt ttcttgcctg gagtttgtcc ggttaagcta acttattgat
     9901 tttgatcaca atattaagcc ctccgaagag ggtttagctg aaagctaaat ccacagagtg
     9961 gatattagaa aagctggtta gcttcctgtg tggctttcca atccagctga tcgctgactt
    10021 ccttctggat caccctggca attcctccct catcaatggt tatctcccat ttgccggaga
    10081 tctcaccctg cggaactaca ctgatttgag tcggctgata gcctccattc tggatagcca
    10141 tgcctgtgct aggtgttggc ctgataccag tgtctactgc taatgttggc cctttattaa
    10201 accaaccctt gacctgatcg aacaaaggac cagtatcacc gccaggtgtg aacagtgcgc
    10261 cagcattgct aacgaatctc cccagctcaa acaatgcgcc ctgtttagga ttctccttgc
    10321 tcatttcctg catcttgcca tcaataaggc ttctttcagc gttagagcct gcactgatag
    10381 ccgctgctcc tgcagctgct ccacttgggg cagatgacat aagcttgccc atacccggga
    10441 ttttgcccag gagagctatc agttgggtga caagtgaaac aaatccggtc agaggggaaa
    10501 gcagtgtagt aataactttc agaccagcta agccaacaac cagttttccg acaagctcgc
    10561 caacctccga tccggtcatg ccgaactctt tagccttaac ctcgatgtaa cccttcgcga
    10621 accctaccca atacatgatc tcacgcatgg cagcaccgac tgcctcacca aactgttttg
    10681 ccgcgctccc gctgccgtcc atatcggtcg taaggtcttc aaagatagag atcagggatt
    10741 cagataagct aataacgcca tctgccggat ctacaacacc cataaactga atcgccagct
    10801 cattaaagct gtttttcatg cgctgcagtg ctacttctgg ctgcttcata gattcagcaa
    10861 aagctttagt ttcttttgca gcaccccaaa ttttcaccag ggaaggaagt agcgcgtcca
    10921 gggtgatttt gccatctttc atggccttat cgaattcttg ggcattcttg atgattccgg
    10981 catcgatagc ggcctgttgg aagtcctgtt gtgagcctgc aaaggcttcc gatagctgtc
    11041 cgcgcaactc ttcggcctgg acctttccct tgcttgccat ctgctgaata gcgacaagtg
    11101 cccggttttg ttgctcgcca gtggcaccta ctgagcggcc tttgatagcc atggcctcaa
    11161 acaatgcctg tttatcactc tctttcatgg ttggagtaga ggcagcaaag cgcatatagc
    11221 caccttccat ctcacgctga tccatgccgt aggcgttggc tgtattcttg ataaaagcca
    11281 tctgttgatc agtcttagct ttatcatcct tgttttgggc catcaaaccg aagtaggcac
    11341 tgttgcgatc tgcaaggctg ctcatagcac cactaacagc acgttgaaca ccccagcctg
    11401 cggcagtcag gccaatggct atcttgccaa gtgtggaatt tagtttcccc aggttgtcat
    11461 gcagcttgga tcctgcgacc gcagctctgt ctagtctgct ctggaatttt ccggcttcag
    11521 ctgttgccat cttcatttca gatctgagtc gggccatcct ctgattatag gtctgcaggt
    11581 taatagcccc tttagcataa tcagcattaa ctctttgaat gcgcccctgg aactgggaga
    11641 cgatagagcc atcaaacatg ccgctaattc gtgcactgtt ggcccctaca cgtgcttttc
    11701 tgtgctgggt gtcaccaaca ctacgctcta acagtttttg ttgtctggcg gcttctctgg
    11761 cttgctgctg gtagaacttg cgcatcgatg ccagctctgg attgttgccc ccggtcagac
    11821 ggctgacagc ctgagcacgt tgtttcttct gtgcttcctg ttgtttcctc tgagctctgg
    11881 caatctgctc ctctacctta gcgcgtgttc tttgtgttct gagtacagcc tgattagatt
    11941 taaaatcagc ttttgccacc tcatctgtag cctttttcca gctcttttta taatcttcca
    12001 atcgttttac ttcttttcgc cagctggatt tatcagaatc ccagtaaata acgtttgtta
    12061 gtctggcgac ttccttcact gccattttac acctcgccct gttttaaaga ttttgataat
    12121 aaaatagccc tttccagggc ctctttatcg atattgtatt tatcggcaag ctcctcgatt
    12181 gataaacctt ctgtgtgaga gccgctctta atgatggtag gtcccctttg tggatttagc
    12241 tgttcagcta aaccttctcc gaaggcaata gaggatccct cttcggagcc ctcgaccagt
    12301 ttttgataaa tcttctcata ggtattcata atcatttccc tttcctgcgg agaggacatt
    12361 tatcacccac agaccataca aaaatataat ggcgcttgct ttcagcttta ggatttaata
    12421 attctgtatg caaatatccg gctgagagca ttttatctac caggttttcc actcttagcc
    12481 cctcctcaaa tgtctgtgcc ttaaaataga gatctggaaa gttctggcct gtatagctac
    12541 cctcctcata taagcgggtg catatatagc ggcataaatc atcgtcattc agatcgctaa
    12601 cgtgtggttt agtgtcggtg ctgaataatg cctgtttaaa aagggagtat ttaaactccc
    12661 catcttccag gaaatcaccc tcttcgaatt caactatcat ttcttcaata ttcataggca
    12721 tatcctctca ttgatgcacc cccaaaaatg gggagtgctt atttatcaat agcttaatca
    12781 agattaacca gtttgagagg tttgacagct tggagcatgg tctgcattgt tcctacgctt
    12841 atttcaaaga gatctgccat gttctgaagg cttgcacctt ctgcctcaat catcgatttg
    12901 gataaggcag caatcacaaa gccatcctgg gcgctgttcc acatgctgat aagaggatca
    12961 tcaccgtgtt ggcgcataag gtcctgaatc atccacaatc tacatgccat aaccaccaaa
    13021 tcgacataga aaacatcacc attagcggac ttgcattcag caaaagcgtg gtgtctgtgt
    13081 tttaagaagt gctctttgtc acgggtaaat gccagggtgt tggtaaactt gccatctaaa
    13141 agatcctggc gaacttttga atcttcagcg tcgaaagctt tgatgacctg ggataataat
    13201 ttagcgattt gagacatttg gaattcctta aattagagcc ccggaagggg ttaaagtgag
    13261 tcgaaaataa ataaatcgaa gagataaaac ttaggctttc tgggccattg ctttctggac
    13321 tttatttcta tggcggcggt agatgaatat aaattcgcga taagcacgac taccgggctt
    13381 aggcaaacgt tgattaagct ccttacgtgg caggatttca ctcaacaaac cccagatatg
    13441 aaagcaggtt acttttaatt cttttgctat ctcttcaata ttcatcccac aattccacat
    13501 taaaagagat ttgcggcggc gatcttcgaa gacatttcta tctcta
//
