LOCUS       CP133393                1308 bp    DNA     circular BCT 03-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KLB_MDR_391377 plasmid p377C4, complete
            sequence.
ACCESSION   CP133393
VERSION     CP133393.1
DBLINK      BioProject: PRJNA987035
            BioSample: SAMN35883625
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Sangeetha,S., Anbazhagan,K. and Shruthi,T.N.
  TITLE     Genomic characterisation of three Multidrug Resistant bacteria from
            critically ill patients by Nanopore technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Sangeetha,S., Anbazhagan,K. and Shruthi,T.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) Microbiology, Rajarajeswari Medical College
            and hospital, No. 202, Kambipura, Mysore Road, Bangalore, Karnataka
            560074, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-APR-2023
            Assembly Method                   :: Flye assembler v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: HS11286
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/24/2023 16:28:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,594
            CDSs (total)                      :: 5,471
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 2,116
            CDSs (without protein)            :: 2,116
            Pseudo Genes (ambiguous residues) :: 0 of 2,116
            Pseudo Genes (frameshifted)       :: 2,002 of 2,116
            Pseudo Genes (incomplete)         :: 150 of 2,116
            Pseudo Genes (internal stop)      :: 43 of 2,116
            Pseudo Genes (multiple problems)  :: 74 of 2,116
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            NCBI analysis of this genome has determined that >30% of the
            predicted CDS features contain frameshifts, which is well above the
            usually observed value of <10%.
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KLB_MDR_391377"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p377C4"
                     /geo_loc_name="India: Bangalore"
                     /collection_date="2023-02-24"
                     /collected_by="RRMCH"
     gene            326..862
                     /locus_tag="RBI21_27970"
     CDS             326..862
                     /locus_tag="RBI21_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WMR25723.1"
                     /translation="MQTQNVVTDKEREIRKRDLEDKDRKNASERRQEQKNQNFTQVYPL
                     GWKRLRELFRKNPGAAELYSMLAENIDGSCGAVVADQTHLASLLGVGRQTISRYVKWLE
                     EQGVLVKIPVAGKVCAYALNPHEVWKGYDNAKPYAAFLTKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
     Typing          327..503
                     /name="Col(KPHS6)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
ORIGIN
        1 cggaacccct gaaggggccc ccacgatttt tcggttgcca atggttaaat tttcaccgtt
       61 ttttgcccga ctgaaaggag ggtgctacct ggagatctcg cagctcgctg tgagatcttt
      121 ttagataaga aggaaaaaat tgtatgttgt tgtttttatt atgggtttat aaatgtgttg
      181 tcgcatccta ggggcgacaa cgttgtcgca tctataggat gcgacagata tacccaacat
      241 gtcgtgcaaa tgcgacatgt tgacacaaaa aaatgacaac tgtatgttac tttcaaacga
      301 catggtgtcg caggagagat agtctatgca aactcaaaat gttgtgacgg acaaagaacg
      361 ggaaatcaga aaaagagatc ttgaagataa agaccgtaaa aacgcctcag agcgtcgaca
      421 ggagcaaaaa aaccaaaact tcacccaagt atacccttta ggctggaagc gtctcagaga
      481 gcttttcagg aagaatccgg gggcagcaga gctttattca atgcttgcag aaaacattga
      541 tggctcttgt ggtgcggttg ttgccgatca aacccattta gcatcgttgc ttggtgttgg
      601 tagacaaaca atatctaggt acgttaagtg gctagaagag caaggtgttt tagtaaaaat
      661 cccagtagct ggaaaggtat gcgcttatgc tctcaacccg catgaagtct ggaaaggcta
      721 cgataatgcc aaaccgtatg ccgcattttt gacgaaaacc ctagtcaaca aagacggcga
      781 tattcagcgc cgaatcatgg ccatgttcca cggaaacgat gcccgtcagg gcatggaagg
      841 cgacacgcca gaagccgaat agattcccgt tcgccaaatc gccgaagccc gcccgcaagg
      901 gcggtacagg ttccacaagg agctgcttca ccactccacc ctgcaaagcc ctaaaacgct
      961 ctcagaacgc acgtaagcca tttttccgcc caaagcccac gtgagcccgt agaacgtcaa
     1021 aaaacgcgtc aaaacgcgtt ttattgcgtt ttacgggtga tttcgtgggt cttgccggac
     1081 tggttcacca ggacggctag aggcggagta ctccgattat ccagactgat ctcatctctc
     1141 aaattcgtga aattagagag atgcgcactt atatgtttcc tcgccgttgg ctcagaaaca
     1201 tgtgcggcct gcggcgctaa aacccgtctg gcaggtggtt gcgctcactg cgtgaacaca
     1261 accaccttcc ccaatttttt ttgatcggtg cgttggcaac aaaaaaat
//
