LOCUS       CP133392                3751 bp    DNA     circular BCT 03-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KLB_MDR_391377 plasmid p377C3, complete
            sequence.
ACCESSION   CP133392
VERSION     CP133392.1
DBLINK      BioProject: PRJNA987035
            BioSample: SAMN35883625
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Sangeetha,S., Anbazhagan,K. and Shruthi,T.N.
  TITLE     Genomic characterisation of three Multidrug Resistant bacteria from
            critically ill patients by Nanopore technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Sangeetha,S., Anbazhagan,K. and Shruthi,T.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) Microbiology, Rajarajeswari Medical College
            and hospital, No. 202, Kambipura, Mysore Road, Bangalore, Karnataka
            560074, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-APR-2023
            Assembly Method                   :: Flye assembler v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: HS11286
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/24/2023 16:28:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,594
            CDSs (total)                      :: 5,471
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 2,116
            CDSs (without protein)            :: 2,116
            Pseudo Genes (ambiguous residues) :: 0 of 2,116
            Pseudo Genes (frameshifted)       :: 2,002 of 2,116
            Pseudo Genes (incomplete)         :: 150 of 2,116
            Pseudo Genes (internal stop)      :: 43 of 2,116
            Pseudo Genes (multiple problems)  :: 74 of 2,116
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            NCBI analysis of this genome has determined that >30% of the
            predicted CDS features contain frameshifts, which is well above the
            usually observed value of <10%.
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KLB_MDR_391377"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p377C3"
                     /geo_loc_name="India: Bangalore"
                     /collection_date="2023-02-24"
                     /collected_by="RRMCH"
     gene            complement(162..560)
                     /locus_tag="RBI21_27950"
     CDS             complement(162..560)
                     /locus_tag="RBI21_27950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMR25720.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            737..1663
                     /locus_tag="RBI21_27955"
     CDS             737..1663
                     /locus_tag="RBI21_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WMR25721.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGYDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1666..2199
                     /locus_tag="RBI21_27960"
     CDS             1666..2199
                     /locus_tag="RBI21_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMR25722.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            2633..3652
                     /locus_tag="RBI21_27965"
     CDS             2633..3652
                     /locus_tag="RBI21_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WMR25719.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     Typing          2634..3408
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          738..1660
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttgagca gcgggctttc cggcggtttt ctcctctcag cccagcaatg gtgcggccta
       61 ccgcatgatt gaggtggtcg tgctcgccgc agtctcatga ccgagcgtag cgagcgaatg
      121 agcgaggaag caccagggaa ccggaacacg gatggaggca cttacgcgtc tgggtttggg
      181 gatgtctcca gatcgtactt aatttcctta agtgcctgta atatcctgtc ttttttatag
      241 catagctcgc ctgtttttgt gtctaccagt cctgcttcga tcagtagccc agccgcgaga
      301 aagagtcggt gattgcgttc tcgtcgttgt ttttttccct cgattcgttt ttggaccgcc
      361 tgttttcgtt cgctctcctg tgcctgtaag tcgttttttt ccgctgttgc cagtacgtgg
      421 aggagtttgc gttcctgttc tgtgagtcct gcttcgttgt cgagcaggtc aataatctgt
      481 ctctgtcgtg cggtctgttc tccgagcttc ctgatgaatc cgatccgttt cttcagccat
      541 tcgatgttgc tgatgcgcat gttcgtggtg ctccctggtg tttttttgcg gtgtgttgtg
      601 gtcgtgcagt atatcgcttg cggtcggcgg gtgcaggggg tatcttccgt gctaattcgt
      661 aaaattagaa ggaagcgcac ttatatgttt ctctccttcg tcgagaaaca gtgcgctcct
      721 gcggagggct gaaaccgtgg caacctatca cctcagcgtt aaatttggtg gcaaggggca
      781 ggctgcaaac cacgccgatt acatcgagcg caaagagaaa taccgcgacc gtcaggattt
      841 ggaatattcc gcacacggaa acatgccgga atgggcgcga gataatccct cacatttctg
      901 gcaggctgct gaccagtttg aacgtgcgaa cggttcgacc tatcgggaac ttgaaattgc
      961 attaccccgt gaactgacag cggaacagcg ccttgaactg gtgcaggatt ttgtacgtca
     1021 ggaagctggc gaacggcacg cctggagttt tgccattcac aatccgaaag cgagcattga
     1081 cggcggcgaa cagcctcatg cgcatatcat gatgagccag cgtgtgaatg atggcattga
     1141 acgtacaccg gaacagtatt tccggcgcta taacgccagg tatcccgagc ggggaggtgc
     1201 gaaaaaggat tccggaagcc tgactctcac tcagcaaaaa gagcagttgc gggaattgcg
     1261 aaaacgctgg gaggtgaagc acaacgaaca tatgagaaag catggctttg aaaggggctt
     1321 cattgactgc cggacgctga aagaacaggg cattgaacga cgtcccgaag tgcatctggg
     1381 ttttgaagct gccggacggc ttgatgatgc ccagcgtcat gacatcatgc aaaaacggtc
     1441 tgaaccggac tattcccgcc atcttgaaca ggcgcgaacg gtcgggtatg acatcaggga
     1501 atggctggac actccggtta agcaacatga actggtcaga aggctgcaac atgatgttgc
     1561 gcgggagcat ggctttgaca tgagagacag agtgagcaga cagaaccgaa ctgaacctga
     1621 acgggttaaa tcaccgtcac gtggattttc tatgggattc tgaacatggc cagcacagaa
     1681 gaaaaattac tcacgctcct gttatcggtg gagcatctgc aacataccgc aatggagcaa
     1741 cagaaagctg ttgctgattc tctggctgtg gcaagggaaa cacttcgcgg atatcgggat
     1801 atttctgacg gtgtggcaaa acaggtcagg gatgaagtca gaaacgaggt gcagaagctg
     1861 gatgttgcag ggctgatagg tgagcgcatc agccaggatt ttcagcggct ggaagagtca
     1921 gtcgcaaaca tgaaggcaaa cgcggatgcc ctgaacaggg atattgcgac aattcacagc
     1981 aatatcgtgc aggaatacaa cacccttaaa gggtatagct ggaaatggct gggtggggtg
     2041 tcagtggctt ttctggttat gttctgtgtg cttggatggg gaatgaaaac aatgctcgac
     2101 aggaactaca acgccacagc ggcggtgtat cagaaagttg tgacactgga agataaactg
     2161 gcggagaaac ctgtcacggg caagagccgg aaaaactgac gcgcttcgct tgtccaacgc
     2221 ctcacgaccc gcgaaaaaat tccgcgtccc gttcaccgtt ttctgatgag aaagtttgct
     2281 gttttgaccc gttcgccctt ctgaacgctc gaaacagatc gcgcatcgct ttcgtgcata
     2341 gttatgcaac gcgctaaaaa cgattctgac gcatttttct ggtttagcct ggtgtttcct
     2401 tgtctttttg cgttttttgc gccagagagc gactgagggc gttttaaggg gggcgtacaa
     2461 cgggagttat ggtaaatgga tcggttttgc gggaaggctc gacaggtttt gttgttgggg
     2521 gtagtgtaag cgactgaaaa acaaacgccc ctgaaattcg tgtctctccg ccaagattgt
     2581 cacgagaata caggggcgag gtcggtcgat aacctggaag gaatcgtaac acatgagcgc
     2641 cgcgcttcaa tacttcgaag aaaatttacc ccaccgcccc tatcacacgg atgatctcgc
     2701 tttcggcctt cgcatctccg gcaaagggcg tgcgcttctt gcgcggtaca tccagcagaa
     2761 ccagcctcat gcgcagttct ggctggtttt tgacgttgac cgcgaggggg ctgcgattga
     2821 ctggagcgac cggaacgccc ccgcgccgaa catcaccgtt aaaaatcctg tgaacggaca
     2881 cgctcacctg ctctacgcac tcaacatcgc cgtgagaacc gcgcctgatg cctcggttaa
     2941 ggcgctgaaa tatgccgcag cggttgaacg ttcgctgtgt gagaaacttt gtgctgatgt
     3001 gaactacagc ggcctgattt gcaaaaatcc gttccacctg gaatggcagg tgatggagtg
     3061 gcgcgaggac gcctataccc tcgatgaact ggcagattat ctcgatttga gcgcctcaga
     3121 gcgtcgtagc atcgataaac attacgggat ggggcgaaac tgccacctgt tcgaaatgac
     3181 gcgcaaatgg gcttacaggg cgattcgtca gggctggcca gcgttctcac agtggcttga
     3241 agcggtgatc cagcgtgtcg aaatgtacaa tgcatcgctt cccgttccgc tttcactggc
     3301 tgaatgtcgg gctattggca agagcattgc gaaatacacg cacaggaact tcacgccgga
     3361 aactttcgca cagtatgtgg ctgatacgca cacgccagaa attcaggcag cacgcggtcg
     3421 caagggcggg aaggccaatt cgtcagaaaa tcagtcggac aaagggaaaa aaagcgccgc
     3481 tgttcggtgg actgctaacg acgacaaacg tcgtagggcg cttgatatgt atatcttggg
     3541 agcgtcaacg gaagatattg cagtggcggt tggtgttagc agccggacta ttcgacgctg
     3601 gatggataat tctggagaat ggctaaccaa gaaacaaata attaaatgtt agttaacaaa
     3661 gaagtaacac taccccccgt tttttgttaa tgggtggtta aattcgcgga catgaacaag
     3721 cctatatcag ataacagcgc ctttcggcgt c
//
