LOCUS       CP132212                4863 bp    DNA     circular BCT 14-AUG-2023
DEFINITION  Enterobacter hormaechei strain 2022CK-00285 plasmid unnamed3,
            complete sequence.
ACCESSION   CP132212
VERSION     CP132212.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN27992995
KEYWORDS    .
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/08/2023 15:50:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,042
            CDSs (total)                      :: 4,923
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 29 of 116
            Pseudo Genes (incomplete)         :: 87 of 116
            Pseudo Genes (internal stop)      :: 14 of 116
            Pseudo Genes (multiple problems)  :: 15 of 116
            Pseudo Genes (short protein)      :: 2 of 116
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00285"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022-04-19"
     gene            complement(431..697)
                     /locus_tag="M2O51_25175"
     CDS             complement(431..697)
                     /locus_tag="M2O51_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLQ00829.1"
                     /translation="MSVYCVSYDLNQAGQNYNALYDELKKSPGWCHPLDSTWLISTGET
                     AQQLSDRLRRHLDNNDTLLVIGVTKEYAGWLTQDTWDWMRTDI"
                     /name="hypothetical protein"
     gene            1392..2690
                     /locus_tag="M2O51_25180"
     CDS             1392..2690
                     /locus_tag="M2O51_25180"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WLQ00830.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            2763..3131
                     /locus_tag="M2O51_25185"
     CDS             2763..3131
                     /locus_tag="M2O51_25185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLQ00825.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(3368..3481)
                     /locus_tag="M2O51_25190"
     CDS             complement(3368..3481)
                     /locus_tag="M2O51_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WLQ00826.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            3897..4088
                     /locus_tag="M2O51_25195"
     CDS             3897..4088
                     /locus_tag="M2O51_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WLQ00827.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4088..4510
                     /locus_tag="M2O51_25200"
     CDS             4088..4510
                     /locus_tag="M2O51_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WLQ00828.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3369..3776)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 tagtgaagat tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat
       61 tgccggccgt cgacgaaccc gatttctgaa cgtgctgaaa accggttttc cggctgcgcc
      121 ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc ggatgggaag
      181 aacgccggaa acaggactta ccctggctga atggcgtgaa ttttcgcgct atgcacttgc
      241 gtgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg
      301 gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tgaggccgtt ttagcggtgc
      361 gtacaattag ggggttatgt taaatccaca ccagctacag acgtaaaaaa agccccgcag
      421 ggctttatat ctatatatct gttctcatcc aatcccatgt gtcttgggtg agccatcctg
      481 cgtattcttt ggttacacca atcacaagca aggtgtcatt attgtccaga tgcctacgca
      541 aacgatccga cagttgttgc gctgtttctc cggttgatat tagccatgtg gaatctaaag
      601 ggtggcacca ccccggcgat ttttttaact catcatatag tgcattgtag ttttgacctg
      661 cttgattcag gtcataagag acacagtaaa ctgacataga accatcctct ttaatcaaat
      721 gtggggcagc ccgttagggc tgcagtcaca acttacttct tcttttcacg attaattgga
      781 attttgaaca tcccaccgtt tggacgggtg atccgtttgc cctttactgt gatataaggg
      841 cgaaaaatgt atgcctgaga ctcgtctgtc tgcatggtgc atttcctatt tttcgccaca
      901 tgagtgttga tttttgctat caaaattgta ctgtagcaac caacaaatct tgtggctggc
      961 tttatcaagc tcttgaggtg gtactcaagt gccgcatttg tgtatggttt gttacgggtt
     1021 agttctggtg gtacaggcta acccacttac ttcaaaaaac gtcgtttttc tcctttcatg
     1081 cgtcatttcc tagatgtttc ttcactgtat cagctccgct gagcataaat gctacaaaga
     1141 gaaaccatcc ccatcctgaa accgagtgta aagctagcac tgctgagctg acaactgcgg
     1201 ctaaaggaac catttggtat agaaaaaatc tcatagaccc cctgcattta gcttttttta
     1261 acaagtgttc cataacgtgc tatctattaa acgcacctgt tgttttagaa cgcttgtatg
     1321 ctatccttaa gtcctgattt tgtcaatatt tactcatttt gttgtttaag gatggcgtaa
     1381 ggggtgttct tatgatggtt aattttagtc tttctaacgc attttccttt atggatgctc
     1441 aaaatatgag catgttggcg gtgtcatccg cgaaagacga cataaacttg gctaacgtgg
     1501 tttcagtgag tgatgttcgg atactgaaat ctgctctgat atttggggct aacgctagcg
     1561 ggaagtctaa tctcatgaaa acaatttctt taatgaaaga tattgttttg cattccgtgc
     1621 aagctattga aagtagcatt acaaagaacg taataccgtt tttgcttgat gaggatggac
     1681 caaataaacc atctgaaatg gaaatgactt ttatttcaga tggggttaaa tatagatatg
     1741 gattgtctat ttttcagggg aaaataaaag aagaatggtt gtactacaca ccaaaatcaa
     1801 gagaaaccat tctctttgaa agagatggtc tttctattga ggtaaataag gcggctttta
     1861 gtgagtcaaa cctttttatc aaagaagggg ttattcaaaa aacaagggag gatgttccat
     1921 ttgtttcggt tgttgctggt tttaatggtg agcattcaaa acgagtagtt aattggttta
     1981 acaatatcgt attcctttct ggttctaatg aagggatgtt tacagcaatt accatgaagt
     2041 taataaatga gtctgaggat tttaaggctt ggctaaaaaa aatacttcca gcattccaga
     2101 ttaatgatat ttacctcgat gaggtcgcac ctgattttcc atttggaaat ataaaaacag
     2161 atgatgataa tttaaaaaat ttatttagtt caatacaaaa ttttgcaaaa aaccagaagg
     2221 ttttttctct tagagtagtg aaaaaaacag ctagcggtga gatttcagta cctattgatt
     2281 ttgagtctga gggtactaaa aagatagttt atcttcttgg tccaatctac aatgccatga
     2341 taaacggtaa cttattgtta gttgatgagt ttgattctaa gtttcacacc cttttgtcta
     2401 agtttttgtt tagaatattc catgcgcaaa gcgaggggct tagccaaata atagctgctg
     2461 ttcaggatgt taatctaatg aacactgatt gttttagacg tgaccagatt tggtttgttg
     2521 ataaagatat aaaatcagga agtagtcatc tctattcttt ggttgagtat aaagagaagc
     2581 agcgaacttt aaaatcatcg tatgggcatg attatttatc tggtgcattt gatgctatcc
     2641 cattgtttga taatttagat gaatttgaaa aacttatgcc tgagggatga tttatgtcaa
     2701 agagaggccg cggtcttcgt agctcttcga ggaaagagga aacaaaatcc tcaaagagca
     2761 ttatgaaaac tacagataga tttatttctg ttagttttag atattttcat aacgtcgatg
     2821 atattggtca gtctattgat tcttggaggg ttgacggcag tttgccagat ttaattgaaa
     2881 agcttgttta tcttacagct aatgggataa caaaagtcag ctctgatggt atttttacta
     2941 actacactcg atttccagat tcagcagtta atgattttaa atgccctgat gaaatatcag
     3001 ttgatgagaa ctggggtgtt ataaaaaata taggaggcca gaaaagaagg gttgctggtt
     3061 ttttaaagga taatgtgttt tatatagttt tttttgataa ggatcatcgg ttttggaaga
     3121 gcaggaaata ggcttagtta cgattgtact gtatgttgag gcaagatctt cttgagatca
     3181 ctgtgttctc gtcgtaatct ctcgctctgt aaacgaaaaa accgccttgg cgggcggttt
     3241 tttcgaaggt tccaagagtt ggcgctctta gaaccaaggt aactggcttg gaggagcgca
     3301 gtaaccaaaa cttgttcttt cagtttagcc ttaaccggcg cataacttca agactaactc
     3361 ctctaaatca gttaccagtg gctgctgcca gtggcgcttt tgcatgtctt tccgggttgg
     3421 actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca
     3481 tacagtccag cttggagcga actgcctacc cggaactgag tgtctggcgt ggaatgagat
     3541 aaacgcggcc ataacagcgg aatggcaccg gtaaaccgaa aggcaggaac aggagagcgc
     3601 acgagggagc cgcctatcag aaatgatagg tatctttata gtcctgtcgg gtttcgccac
     3661 cactgatttg agcgtccgtt tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac
     3721 ggccgctgcg cggcctcctc ttttcctcct accttgatcg ctcggttttc tcgagctttt
     3781 ataagaatgg tcttgccgct cgccgcagcc gaacgaccgg agcgtagcga ctgagtgagc
     3841 gaggaagcgg aaaagagact ggttttacac tgagcactga cgctctgagg cttcttatga
     3901 cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag tctctgctcc
     3961 tgctggagaa gcttaacgag cttgctctcg atgtcgaggc cgacctttgt gagaaactcc
     4021 acgatgatgc ggagcatctt ttccgtacac tgtcctcgag actggatgaa ttacaggatg
     4081 gtaactgatg gccagagtgt ctattagtga agcggcacgg cttgttaagg ttagtcgccc
     4141 cacaatttac aagatgatta acagtgggaa gttaagttac acctctgttg taaagcacgg
     4201 taaggctatt aaagttattg atacttcaga gcttattaga gtgtttggtt ctcttgatgg
     4261 tgtcatcgat actgtaaaat atgatgtaaa aagtgacgca gaatctacag gtgttaacag
     4321 cgttggttta catgatttac aacatagaat cgctttactt gaagctgaaa acgatggtct
     4381 taaaggggct gttaaggctc gagatgagca tattgattcc cttcgtcagg ctatgcagct
     4441 tctggagcac aagcacgaac cttcttcacc accccattcc ccgtggtgga aattctggaa
     4501 aaaatcctga ccccgtcatg cttccgtttt cctctccggc catcccgggg agttcctgaa
     4561 ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac ccacacccca
     4621 cgcaaaatta acattttgca gatttgtatt taatatcagt gggttacttt cctgaaattt
     4681 cgttttttaa attttattta tatagatccg actctatgac gagtcgtcat agagtccccg
     4741 gcgagtcgtc atagagtcgg taacgactca ggactataaa aacaataagt taataacgac
     4801 ccgactctag acacgactcc ccgtttggtt gttttttaga ctcggcgact ctgggtacga
     4861 ctc
//
