LOCUS       CP128784               11970 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain ARLG-4365 plasmid pARLG-4365_3,
            complete sequence.
ACCESSION   CP128784
VERSION     CP128784.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878146
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2279833830941x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:26:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,793
            CDSs (total)                      :: 5,671
            Genes (coding)                    :: 5,509
            CDSs (with protein)               :: 5,509
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 63 of 162
            Pseudo Genes (incomplete)         :: 106 of 162
            Pseudo Genes (internal stop)      :: 20 of 162
            Pseudo Genes (multiple problems)  :: 27 of 162
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4365"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pARLG-4365_3"
                     /geo_loc_name="USA:Northeast"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            complement(join(11906..11970,1..307))
                     /gene="tolA"
                     /locus_tag="QU729_28900"
     CDS             complement(join(11906..11970,1..307))
                     /gene="tolA"
                     /locus_tag="QU729_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WJV01786.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(581..826)
                     /locus_tag="QU729_28905"
     CDS             complement(581..826)
                     /locus_tag="QU729_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJV01787.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1471..2958
                     /gene="ltrA"
                     /locus_tag="QU729_28910"
     CDS             1471..2958
                     /gene="ltrA"
                     /locus_tag="QU729_28910"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003964 - RNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WJV01788.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            3063..4748
                     /locus_tag="QU729_28915"
     CDS             3063..4748
                     /locus_tag="QU729_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WJV01789.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4758..5015
                     /locus_tag="QU729_28920"
     CDS             4758..5015
                     /locus_tag="QU729_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WJV01790.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5100..5249
                     /locus_tag="QU729_28925"
     CDS             5100..5249
                     /locus_tag="QU729_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WJV01791.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6733..8697
                     /locus_tag="QU729_28930"
     CDS             6733..8697
                     /locus_tag="QU729_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJV01778.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8697..9428
                     /gene="mobC"
                     /locus_tag="QU729_28935"
     CDS             8697..9428
                     /gene="mobC"
                     /locus_tag="QU729_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WJV01779.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9435..9965
                     /locus_tag="QU729_28940"
     CDS             9435..9965
                     /locus_tag="QU729_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJV01780.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9992..10171)
                     /locus_tag="QU729_28945"
     CDS             complement(9992..10171)
                     /locus_tag="QU729_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJV01781.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10197..10625)
                     /locus_tag="QU729_28950"
     CDS             complement(10197..10625)
                     /locus_tag="QU729_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJV01782.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(10789..11103)
                     /locus_tag="QU729_28955"
     CDS             complement(10789..11103)
                     /locus_tag="QU729_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJV01783.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11091..11369)
                     /locus_tag="QU729_28960"
     CDS             complement(11091..11369)
                     /locus_tag="QU729_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WJV01784.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(11544..11909)
                     /locus_tag="QU729_28965"
     CDS             complement(11544..11909)
                     /locus_tag="QU729_28965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WJV01785.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1..9748
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5353..5931)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8698..9425
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gaattttggc gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt
       61 cagcaatggc gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt
      121 ttcccgcata ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag
      181 cgtagctact gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg
      241 aatgcttaca ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta
      301 aacgcatcgg gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg tcactcttta
      361 cggccacgtt catttacata aacacggctt atcgtatcgt ttttacacca aacggcccgc
      421 catgcagttg cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg
      481 ttttccgggt ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt
      541 ctgagcggtg cgggcaatgc gacgttatgg taaatacccc ttaccggcgg cgctgtggct
      601 gccaggtgaa ggtggtttcc gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc
      661 tggcttcacg ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt tgttttaccg
      721 cctttggcag cgttttgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt
      781 ccgccaggcg cttttcttca gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc
      841 gtctcaggtt tcccgaacag tttaaccgca ggtcctctcc tcatgccagt tagctttcag
      901 atgtgtctat accatctgaa atgtaagtgt gatgaatgtc ttaaataata aaatttgttg
      961 ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt
     1021 gtgcctgaag atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta
     1081 ataattatac tgtgtatatg tacagtgtgc gccgggagac cggttagaga tcaaggggtg
     1141 aaagtccccg accattgaag gaccagcaat ccacaaggtc cccgagtcat gcgttgcata
     1201 ccgcgaggta tggggcgaag cgttgacagg ggtgttgaca ggccagccat tgagccacga
     1261 aatgtatatt aaattaccgg gtgccgacgt tgtactgtta acggaaggca acatcatagg
     1321 gtgcgatact gcgagtgcca catggacccg gcggggtctg agaccctggc atgtcaatac
     1381 gatctctacg cgggaaccgg gagatctccc ctctgaccat ctgccagtgt cggagatggc
     1441 ccgcaccggg aagacgagga gtcatagccg gtgatgtacg gagaggagaa gtcggactcg
     1501 ctcatagtag cggcgaagca ggcgaacaac ccgaaaggag cggagtcagt ggagcgaagg
     1561 agcggggcca aggggaacgc ggaacagcca cacatgcgcc ggacacagag ccgggaaagc
     1621 atgtcacaga ggctgtcacg cgtgcgggaa gctgcgaagc agcggaagaa agaacggttt
     1681 acagcattgt tccacctgct gacagtcgaa gcactggaag ccgcattcct ctccctgagc
     1741 aggaaagcgg ccgccggagt ggatggcatc aggtggatgg actacgccgg aaacatgaag
     1801 aacaacataa cagatctgca ccggaggcta catcagggca gctacagggc gcagcccggc
     1861 aggcgtcact acatcccaaa agcggatgga aaacaacgcc cgctcggcat cgcctcgctg
     1921 gaggacaaga tcgtccagta tgcgctggtg aaaatcctga acgcagtcta tgaaaacgac
     1981 tttatggggt tctcatacgg gttcagaccc gggcgaagcc agcacgatgc actggacgca
     2041 ctggccacag ggctggtacg cactaacgta aactgggtac tggatgccga catcagtcag
     2101 ttcttcgaca gggtgagcca cgaatggctg atcaggttca cagagcatcg gatcggcgac
     2161 cggagggtaa tcaggctcat acgtaagtgg ctcacagccg ggacgtcgga ggagggtcaa
     2221 tggcgagcaa cggaggaagg caccccacag ggtgcggtca tctcaccgct gctggcaaac
     2281 atatacctcc actacgtctt cgatctgtgg gcgcatcagt ggcgacgtcg ctatgccaca
     2341 ggcaatgtgg taatggtcag atacgccgat gacatcgtca tcgggttcga caaacgatac
     2401 gatgcccggc gcttccgtat agccatgcag cgcagactga gggagttcgg actcacggtt
     2461 cacccggaga aaacccgtct gatggagttc ggccgcttcg ctgccgaaaa ccgtgccatc
     2521 aggggaaaag gcaaaccaga aacgttcaac ttcctcgggt tcacgcacat cagcgggaaa
     2581 gatcgcaacg gcaggttcat gctgatacga aagacccgcc gggatcggat gacggcaact
     2641 ctgaaagcca tcaaagacgg tctgcgaagg cgctggcatt actcaatccc cgaacaggga
     2701 aaatggctca ggagagtggt tcagggatac ctgaactatc actcggtacc gggcaacttc
     2761 cccaccatgc agaagttcag gacacacgta acaaacctct ggcgccgggc gctcaggcgc
     2821 aggagccaga aggatgatac gacctggacg aaagcaaaca aactggcagc cgcatggcta
     2881 ccaagggttc gggttcttca tccatggcct gtggagcggt tcaccgccag acacccgagg
     2941 caggagcccg gtgcgtaaat cgcgcacgcc gggatctgtg cggggggtat ccggtaacgg
     3001 gtatccctac cgcgacatgt tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg
     3061 gtatgagtgg tggagacggt cgaggtccgg gtaattcagg tctgggacat aatggtggtc
     3121 aggccagtgg gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta
     3181 cggatccaaa cagcgggccg ggctggggta cgacgcatac gccgaatgga gatattcata
     3241 actataatcc gggggagttt ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca
     3301 gcggcaatca tagcggtagc tctggtggcg gacagtcttc ggccactgcg atggccttcg
     3361 gtctgcctgc cctggctact ccgggggctg aaggactggc tttatcagtt tccggcgatg
     3421 cgttgtcggc cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc
     3481 tgtgggggat tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa
     3541 tgatgtcaaa aattatgacg tcattaccgg ccgatgcggt gacggagact ccggcaagta
     3601 ctttaccact ggaccaggcg acggttcgtg tcagacaacg ggttgtggat gtggtgaagg
     3661 atgagcggca gcatattgcg gttgtcgcag gtcggccaat gagtgtccct gtggtggatg
     3721 cgaaaccgac aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc
     3781 aggtgagcgt accgaaaggt gttccgacag cgaaagcccc gccaaaaggc attgttgctg
     3841 aaaaaggtga ttcacgtccg gctggtttta cagccggtgg taactcccgt gaggccgtta
     3901 ttcgtttccc gaaagagacc ggacagaagc cggtttatgt gtcggtgaca gatgttctta
     3961 ccccggcaca ggtaaaacag cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg
     4021 ccgctcatcc ggaagagggg ctgaaaagag actatgataa agcgaaagct gagctggatg
     4081 ccgaagataa aaatattgcg accttaaaca gccgcattgc atcgacagag aaggcgctcc
     4141 ccggtgcaag ggctgctgtt caggaagccg ataaaaaggt aaaagaggca gaggcaaata
     4201 aagatgattt tgtgacttat aacccgcctc atgaatatgg ctccgggtgg caggatcagg
     4261 ttcgctatct tgataaggat attcagaatc agaatgagaa attaaaagcg gctcaggcat
     4321 ctttaaacgc aatgaatgaa tccttatcca gagataaggc tgcacttacc ggggcgatgg
     4381 agagccggaa acaaaaggag aaaaaagcga aggatgcaga aaataagtta aatgaggaaa
     4441 agaacaagcc tcgcaagggg actaaagact acggccatga ttatcatcca gtacctaaaa
     4501 cagaagacat aaagggactt ggagagttaa agcgaggtga tcctaaaaca cctaaacaag
     4561 gtggtggtgg taagcgggcc cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg
     4621 attcccagca cggtgagctt gaagggtatc gtgccagtga tggcgaacac ctaggcgcat
     4681 ttgatcccaa aacgggtaag caggttaaag ggccagatcc aaaacggaac attaaaaaat
     4741 atctttgaga ggtaaatatg gggcttaaat tacgtttgac atggtttgat aagaaaactg
     4801 aagagtttaa aggggaagag tattctaaag actttggtga cgatggttct gtcattgaaa
     4861 gcttgggaat gcctttaaag gataatatta ataatggtga gtttgatttg aagaaagaat
     4921 gggttccttt actacagcca ttttttaaaa atcaaataaa acctgatgtg gataattgtt
     4981 atatagcttt tgattatcgg gacggtaact ggtaacaaaa aattatcaag gatgatttga
     5041 aatactgact tccggtgttc agggatgaat gccggagtct ttaaatacag ggaaagtata
     5101 tgaaaaaggt aaagactatt tttcttttta tattgattgt tgctggcttt cttctggccg
     5161 catgtcaggc aaattatatc cgggatgttc agggcggaac ggtggcacca tcgtcctcct
     5221 ctgaactgac ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt
     5281 ttttctgcgc gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat
     5341 cgaaggttaa gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag
     5401 caaccaaatc tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg
     5461 tgtttagcta aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt
     5521 tttgcgtgct ttcccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg
     5581 ggctgaacgg ggagttcttg cttacagccc agcttggagc gaacgaccta caccgagccg
     5641 agataccagt gtgtgagcta tgagaaagcg ccacacttcc cgcaagggag aacggcggac
     5701 aggtatccgg taaacggcag ggtcggaaca ggagagcgca agagggagcg acctaccgga
     5761 aacggtgggg atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt catggttgag
     5821 cctcacggct cccacagatg caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg
     5881 gcggagccta tggaaaaacg ccaccggcgc ggccctgccg ttttgcctca catgttagtc
     5941 ccctgcttat ccacggaatc tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt
     6001 ctcacgcccg gagcgtagcg accgagtgag cgaggaggcg caatttcccc ggcacctgca
     6061 agacggtcac cggacgcgct gatgtgggat attgtcccct gattaagtct ttctattccc
     6121 tgacgatcaa ttatcaggat aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta
     6181 acctatctct gttgcttttt tctccttgcg gagaaatgca agtcatcccc gctggggcgt
     6241 ggctgacttc aggggaaaag accttgctta cgctggctgg acgccaccga cgcacggtct
     6301 tttcccctct ttctggctct gtccggctgg acgccggcca cgccagaaat tcacccggcc
     6361 cccgtcaggc tcacgcctga ccacccacaa aaatccacgg tgctggacgc acctgacgaa
     6421 gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct cgctatggcg aactctgacg
     6481 ggggccggaa caccggaaaa gaccgcactg gggtgccgcc ttttcctctc acacgctggg
     6541 gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc
     6601 tccgcggtga aagctgaacg caatcaccac ggacgtgacc gccgccgcca tgccttgggg
     6661 catgtccgcg gcttgtgcag cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag
     6721 gagagagatt gcatgtttaa tacggattcg cttgcctggc agtggcatat cccggagctg
     6781 gcccggcaga tttacccgtg ggtcagctgg ctgtaccggg agtattacag tctggccggt
     6841 ttttttctgg ttatcggcct gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt
     6901 tttgtggggc tgagccataa ggcgcggcgg gagtatgccg atcgctataa gcgcgataat
     6961 aaccggactg tgccattttc ggtgctggcc tcgctggatt cacccgggcg gatggtcagg
     7021 gcacggcgac atgtctggaa ctacgcgtac tggctgaccg gcgctgccgg tctggccctt
     7081 ctgggggggc tggttgtcgc cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg
     7141 gacctctatg cgaacgcaga ttattacctg ctgcagccgg ctaacctgtt cgggatagcc
     7201 tggtgtgccg gagggcttgt cgccggcgtc cttctggcgc ggctgctggc gttcctgctg
     7261 cttgatggtc attttgcggc cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc
     7321 cggcgcagca gccagcggac cggagaaatg acagacgtcc gccacctgca tttcggggag
     7381 ccggtaccgg ttaacgcgct ggccgatttt tccacggagc aggcccggaa acagcaggcg
     7441 gtgtttctgg gtaaggatga gcagggccag ccggtgctgg tccctcgtga tacctggcgc
     7501 aagaccaata ttcagatcct cggcctgccc gggagcggca agagcgcgat ggccaccaac
     7561 gcgctcattc gctgcgtacg cgatttcggg gacgctgtgg tgtacttcga ccccaaaggg
     7621 gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg acttcaccct gctggatctg
     7681 cggccgggga aaccggcgca gctgaatctg ttccgcgatc tcgaccagta cgccctgaag
     7741 aatctgctgg ttgccgggtt taacctgagt gagaccggcg acgtggcgga ccactaccgc
     7801 atcagcgagc agaaagcggc gaagctgatt gcggagcagt tcccgcaggg ggcaaacatt
     7861 cagcaggtgc tggcggcggc gtacgcgttg ccggaggccc tgaaaaagga cgtgaagggg
     7921 ctgatcacca agctggagaa cgtggccgac ctgagcgtcc tgcagacgga cagcggtatc
     7981 gatgtggcgg ggataatcaa cggcggcggc tgcctgtacg tcatcggctc gatggacgat
     8041 gaggcggtga tccgggtgca gaagatgctg tttgcccgct gcgcgcaaat catcattgcg
     8101 cgggatgagt tccggaggtg gccgcacgcg agcatcatgc tcgatgagat taaatatctg
     8161 ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca gccgggactg caacctgctg
     8221 ctcgcgcacc agtcgctggg cgacttcggt cagtgcgggc aggatctgcc cgctgatttt
     8281 gtgaaaacca cggtactgga taacacgccg atccgctggt tctatcgcgc ggccagccag
     8341 gagtccgcgc agtgggcggc gggccagacc ggggagatcc gggtggacgt ggagcgccgc
     8401 cgggccagcc gggaggcggg gaacgtggag cacatcagcg gcgacagctt catccagaag
     8461 gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc tgcctgacgg gttcgcggtg
     8521 atgaccgggc tgggcgtggc gcggctggcg ttcagcagcc cgctgcgggt tgagcgccgg
     8581 gaaatttcgc tgaaaacctt cccggtgctg gcgaaaacgg acccgctggc ggagtacctg
     8641 cctccggccg acggcggcgg accaaaagcg gatgatgatt ttgcggggct ttactgatga
     8701 cactggagca gtacaacgtc agccacgcga agcggcaggc gcggaacgcg gagaaaacac
     8761 ggctgaccct gcgctggctg cgggaagagc tgtgttccac ggcggagctg gttgcgcgac
     8821 ggctggggat tgcggcggta cagccggttt accgcttcct ggacagtctg gtggcgaaag
     8881 ggctgctggt ccgggcgaaa tacccggttg acggccgtca ggtcagcgtg tgggggctga
     8941 cgccgcacgg ggtggcgttc tcgttcgacg aggacgagcc gctgacggac gttattccgt
     9001 ttcagccgtc aagggtgtcg gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc
     9061 gcctctccat ggagggccgg ggggccaccg gctggcggta cctgcaccgt atggccctga
     9121 aagggatgaa agtgccggac gcgctggcgg agctggacgg cagaacggtg gcatttgagg
     9181 tagagcgcac cgtcaagagc cgcaggcggt atcaggaagt ggtgtccggg tatctgttca
     9241 accgccgggc gaacggcatc gatgaaatct ggtacatctg ccctgaccgg ccgacgcttg
     9301 tccgggtaca gcgggcgatt ctggcggtgg atgaaatcgt gaatccacag accggcgagg
     9361 cccgcaaaac ggtggagctg gaccgggaga ggctgttcgc gtgctttaaa tttatgacaa
     9421 cggagtgagg gattatggcg ctgtacatgg tatttattga ggacgtggaa gacaaagacg
     9481 gcgagcgctg cattatgcca accagcgagc atctggaaga catggtggtg ctcgcctggc
     9541 agaccggggc gtatgttttc tgttttgtcg accgggccca gcatctggcg atgatgaaat
     9601 ccatcgggat gtatgacgag cggctcaaca gcgacgaaaa agccacgtac tttttcaccg
     9661 gtgaaaatcc gcaatgggaa cgggacaaac ccacaggaat gaagctgtgt gattacctct
     9721 atcgaaaggt ggagcggttc gccatcgaga acggtgtgca ggtggttgat gccgggaagt
     9781 gctcaccgga gcagattaac gcgatgattg aggtggtcag cgagcggcac ggggaaagcg
     9841 cggtgaacga gaagctgaaa gcggagctga tgcgggaata taacagccgc tattttaacg
     9901 tgccggtgcc tgaactgccg gaggacaaac ccgcccgccg gtggtggccg ttcggcaaaa
     9961 aataacgcca gcaccggcat ggccggtgct gttattcttc cccgagctta tcccgcatca
    10021 cctgccagag tgtttccgca tcctcgtgaa gccgttcgca catgtcggcc ggggtatcca
    10081 tgtccagctc gttcagcttt tccagcaaca gcagcgactg atccttaatg aatttagcca
    10141 tattgagggc agttttttcc tgcttcgtca tgttctccat tcccttcggt aagttctcag
    10201 ctaaaccagc cggaccagaa gcgttttttc ttctgtgtca gttctgttgt caatctgtcc
    10261 acttcggcct ggagtttccg gcgctcatca tcgtgctggc ggcgttcttc ttcccgggat
    10321 gttttatctt ccagcatcag ggtgacagcc tgccggagat cggccagttc cttttgcatt
    10381 gccagaagct gttcctgtgg gaaatttttt tgtgtatttt cacggtgtga aatgccggga
    10441 atttgtttct cagggatatt cacatttccg tatacacgga tcagttctga cacatccacg
    10501 acagggttgt ttttcccgtc acgtgacacg gtaacctttc cctgttttac atgattgtat
    10561 agcgttcttc tggtaatgcc cgcggcttgt gctgcctgtg aaaggttgag caatgttttc
    10621 gccactgtat acccctgatt ttatcgggtg tgaaaggtgt gtaatttctc acggtaaatg
    10681 tatcgctgaa ttttacccgt ttttccgttc aggatgtggt tttctgacgc gctgcgcttg
    10741 tcaaaccggc gacggccacg caatgaattg cgcccccgtc atcggttatt agaggctgtc
    10801 tgcccccgga tacccggatt ctttctgatg cctgacggtg acgatcacaa tctggtccat
    10861 ttcccggtcg tggcggtaga gcatcacata gccactgtcg ccaaacccga tcaccagttc
    10921 cttatattcc agcggcagaa acggcaccgg gcggccaatg tctggcaatg ttttcagttg
    10981 ctggatagcc cggacgatca cctcacctcc ttttctggct gccagccgat ttttggtttt
    11041 gagaaagtcc tgaaggcgtt gtaaatcgcg ctgtgccagc gcggaaatcg ttacctgtgg
    11101 catggcggag catcctgctc gttctcagtt ccccaggtac ttatccaggc ttccgcttct
    11161 tccgcagtaa ggtgcagacc cgtttcctga tagtgctgcc atgcggcctg tccgtcccgc
    11221 aggtactggc tgcgcttctc ttcccggtcg atgtattcgg tgattgcctc cagcattagc
    11281 gcgtgcgctg agcggtgccg gtcatcggcc agcgttttca gccggtcctt aagttcttca
    11341 tcgagcctga tggatgttgc tgatgacata aaagcccctt tgtagtcata tgtattacac
    11401 atgactactt taatccgtgg cggcgctatt tgccagaaat acggcttttg ctgcccattt
    11461 ttcagcttcg cttccactaa tcagggctgc tacaggctca ctcatcatcc aggactattg
    11521 cagcctgcgg gttaaaggac ctgttactgg cggcaaccat catcattgag cttttgggtg
    11581 gtgactggtc gcggatcctg taagccgtca tttttcagac tgaattcaaa atttataacc
    11641 tgtcgcattg ccgggtgccc tgcgggatat agccagtgcc tcattgccgt cagcgtttcc
    11701 cgaaaaaaaa ggtgacagcc ggtaagcgac acgttgtcca ctcgtccatc gtccagaata
    11761 tcaaacgtgg cggtcacctg accttcgatg cgttttgctg cggcctgtac cgggtaggtt
    11821 ggctgcagtg tggttacggg ttttagtggt atcgcatgta ccggagtgag gaacatggcc
    11881 gccagcactg tcagagtaaa agctttcatg gtctgaaatc cagaggggcg tttttgaata
    11941 tttgccaggt attttcatcc ggtgccggcg
//
