LOCUS       CP128780                8514 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain ARLG-4803 plasmid pARLG-4803_3,
            complete sequence.
ACCESSION   CP128780
VERSION     CP128780.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878147
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 55.1961744573578x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:49:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,511
            CDSs (total)                      :: 5,385
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 68 of 147
            Pseudo Genes (incomplete)         :: 82 of 147
            Pseudo Genes (internal stop)      :: 23 of 147
            Pseudo Genes (multiple problems)  :: 23 of 147
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4803"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pARLG-4803_3"
                     /geo_loc_name="USA:South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..114
                     /locus_tag="QU743_27515"
     CDS             1..114
                     /locus_tag="QU743_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WJV07037.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(321..797)
                     /locus_tag="QU743_27520"
     CDS             complement(321..797)
                     /locus_tag="QU743_27520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJV07038.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(797..1441)
                     /locus_tag="QU743_27525"
     CDS             complement(797..1441)
                     /locus_tag="QU743_27525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJV07039.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            complement(1443..1667)
                     /locus_tag="QU743_27530"
     CDS             complement(1443..1667)
                     /locus_tag="QU743_27530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJV07040.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            complement(2524..5529)
                     /locus_tag="QU743_27535"
     CDS             complement(2524..5529)
                     /locus_tag="QU743_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WJV07041.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            5693..6250
                     /locus_tag="QU743_27540"
     CDS             5693..6250
                     /locus_tag="QU743_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WJV07033.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            6433..7293
                     /locus_tag="QU743_27545"
     CDS             6433..7293
                     /locus_tag="QU743_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WJV07034.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(7486..7908)
                     /locus_tag="QU743_27550"
     CDS             complement(7486..7908)
                     /locus_tag="QU743_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WJV07035.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7908..8099)
                     /locus_tag="QU743_27555"
     CDS             complement(7908..8099)
                     /locus_tag="QU743_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJV07036.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     ARG             6434..7290
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..114
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="100.0"
                     /evalue="2.98e-37"
     Typing          8221..8514
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.27"
                     /coverage="99.0"
                     /evalue="2.92e-129"
ORIGIN
        1 atgcacgaac cccccgttca gtccgactgc tgcgcctttt ccggtaacta tcaacttgag
       61 tccaacccgg aaagacacga cgaagcgcca ctggcagcag ccactggtaa ctgatttaga
      121 ggagttagtc ttgaagttat gtgccagtta aggctaaact gaaagaacga ttttggttac
      181 tgcgctcctc taactagcca gttaccttgg ttcatagagc cgcaaactct tagaacctaa
      241 gaaaaaccgc cccgcaaagg cggtttttct ttttcaagaa gagtactagg tcacacttga
      301 tgacgtcaag acggttttcg ctaaagagga acaaccacgc caccttcatt tctttgaaaa
      361 aaagaggata attccaaaat agttgtagtc acctcaatca aagtaaattc atcattaata
      421 tcaaataaat accacaaacc caagtgaggg taaccttcgt cctttatatc aatacattta
      481 tctggtttgt taacttgctc gatcacttcc gctgcatcaa atgagagata tatggcaggt
      541 ttggttttcc ctacagggat tcttttatgt acatccctta ccattccttc ataataatct
      601 ttatttgaat gataaataga cacataatcc tctgaaattt cctccctaag ataaaaacac
      661 ttaatagttg tagcgccaaa ctcatcttta tgatcttcca taacataacg ccataaatat
      721 gaattatttg ctctgcccgt aaatgcatta atacaaggga ttgatttgag gttatttaaa
      781 gttgataaca cagtcatcac cactccatca ttttaaataa tgatttgata gcaatggagt
      841 tatcaaggct atcgccaata tatgccctac ctgtaacctt aaccactcca gaaacttttc
      901 taacaaagga aaaaataatc tcgttatttg ttttcactat taaatttagc gtgcctttgc
      961 ttttcttagt ttgtttatat ataagcccaa aacactctga ttttttatca aagtaaatct
     1021 gagcgtcttt ttttgcagca agttcagggg catacctaat caactgaaca atagcctgca
     1081 aagatggttc taggtcacca gtgcagaact cgtattctct attataaaca gaaatggcac
     1141 ttcttatttt tttaaaaaac aataagaatt cacctgataa ttctaatgat tcgtaatgtt
     1201 ttaaaattga ctcataaccg tcaccagagc aatctaatgt tttaacatca tcattccaag
     1261 actcccaaac agaattctca acaacaacaa atgctcgacc caaaacttta aacaaatccg
     1321 gatctcttaa atcatttagc tctaacttat tataatcaga aacatcataa cgaacaccga
     1381 gatcaatgct atcattagta atataataat cttcactatc gcagtggtta atgtatttca
     1441 tactaatcac ctgactcaac gaatttattc aatccatcga taaggttctt tgcatgtttt
     1501 ttggttagag ctatggaata aaaaaccctt cctgattctt tttcaggaaa gtccactaac
     1561 tctaaaaaac aaatatcttc agctatccca actcttagag ccatcgggaa tacaggttca
     1621 ttttttcttg gtgttacttt tctttctaaa gaatctttat tgctcattat aataccccga
     1681 tagaaataaa tttaagttta gaacataaaa aataatacca taaaacaaca tctttcatcc
     1741 acaatgctca tttaacatta cattggtcac tttatggaat tttgtggcca tgatttaaca
     1801 taacaccaaa attgtacgca ccgctaaaac ggcctcaggg cgtttctgac gggaaaccgg
     1861 taaaataggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     1921 catgcatgag tatgcacgca agtgcatagc gcgaaaattc acgccattca gccagggtaa
     1981 gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt
     2041 ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcacgttc aggaatcggg
     2101 ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattca
     2161 ccggcgactc ttcactagag tcgtacccag agtcgtcacg tctaaaaaac aaccaaacgg
     2221 ggagtcgggt ctagagtcgg gtcgttgtta acttattgtt tttatagtcc tgagtcgtta
     2281 ccgactctat gacgactcgt catagagtcc ccggcgagtc gtcatagagt cggatctgta
     2341 taaataaaat taaaaaaacg aaatttatag aacgtaaact actgatataa aatacaaatc
     2401 tgcaaaattt taattttgcg tggggtgtgg atatttttag tggtgaggag cgttcagaga
     2461 tctggcgatt tttgggaagg ttcaggaact ggggtctgac gctcagtgga acgaaaactc
     2521 acgttaagca acgttttctg cctctgacgc ctcttttaat ggtctcagat gtcctttggt
     2581 caccagttct gccagcgtga aggaataatg gccgagcata ttgatatgtc cgtggcaaag
     2641 cggggagagg cgtgcgatat cttcatcatt cagtgtttca ccctgcgccc ggagatgatc
     2701 cagggctgcc tgcatataaa tagtgttcca taacacgacg gcgttagtga ccagccccag
     2761 tgtgcccagt tgatcttcct gaccgtcggt atatcgtttt cttatctcac ctttttgacc
     2821 gtgacagatg gctctggcaa cggcatggcg actttctccc cgattaagct gggtcagaat
     2881 gcgccggcgg taatcttcat catcaatata attaagcaga tacagcgttt tgttgatgcg
     2941 ccccacttca atgattgcct gagtcagtcc ggaaggacgt tcacttttca gcaatgaacg
     3001 gaccagcact gaaacctgta ctttgcccag cttcagggag ccagcggtcc ggatcatttc
     3061 gtcccactga aggactattt ttcggggatc tgattgccct ctggcaatat cattcagcac
     3121 gccatagtcg gcatcatggt ccattcgcca gaaaaccgaa gcaccggcat cagccaggcg
     3181 tggagaaaac tggtatccca gcagccagaa aaggccaaag acaagttcgc tggcacctgc
     3241 tgtatcggtc ataatttcgg ttggattcag cccggtctcc tgttccagaa gaccttccag
     3301 cacaaagata gagtccctca gcgtccccgg tataacgatg ccatgaaagc cggaatactg
     3361 atcggacaca aagttgtacc aggtgatccc tctgttatta ccaaagtatt tgcggttcgg
     3421 tccggcattg attgttctga ctggcgtaac aaagcgcatt ccatctgcag atgccacttc
     3481 tcctccaccc catatctgtg ccagtggcag cgttgcctga aaatcaacca gtctggcatt
     3541 agcgctggtg atagtttcag cccgcagata gttcgctttt gtccagttca gccggtgtcg
     3601 ggtcagtgca ggaacatttg atctgatcag tggttccaga ccgatattgc aggcttcagc
     3661 catcagcacg gcgctgatgc tgacgggcag atcatcaact ctggcactgg cttcactagc
     3721 atggaaaaac tcatcagcaa atccggtatg ggcgttaatt tcgagcagca actccgttaa
     3781 atccaccgga gggagtagat cactgatcat tttgctcagt cgtttcagac tgtccggctc
     3841 atcaagactg gcgaggggag aaattgtcaa ccggggcttc gggccagaaa catcgagttc
     3901 gacagcctca ttttcgcaaa gacgtgcagc aacctgtctg taacgactat caagctgatg
     3961 acccagagat tttattgctt cctgcgggtc tgtcgggtgc cccaaagaac gataaacctt
     4021 aatccggttt gcctgccagt cagcaccctg tagtaatctt gcacgaggat ctccccaccg
     4081 gttactgccg gtaacgtaga catccctccg cctcagacta tcctgcagtt tactgagaaa
     4141 gcagagcgtg tatcccctgc gggtgatatg tttttccttg ttaatcacca gccgtttcca
     4201 tgaccgactg ataatttccg ttggtgcgtc gtcaaaaaac tgccgccgtg agctgaactc
     4261 ccggctgagg tagtcacagg cattcagagt ggtaaccccg gcaggtgcgg atgaaaattt
     4321 aacggtattc agcagatggg gcaggaaacg acgaacgcgc ccgtactgct ccaccatttc
     4381 ttcatgaaaa ttatcgtctg agggccgggc aatttcacgg acaagcgtga tgatttcagc
     4441 cagcttttgc cttgggatgt agctgaacac ctcagcacga atcgattcgt ccggtgtttc
     4501 ttctttcagc aggtacgaac atgcgctggc gagcgccaat gcagatttat ccagatcctt
     4561 cagcgagcgg agccgttttt tctgcccaat ctttctggcg tcacggatga taacggccag
     4621 catggcgtcc agaacgtcca atgcatcatc cagcgccagc gtttcccatg caaggacaaa
     4681 ggcaaccaga accgccatcc ttttctgcgg tgacatcctg gcaatattga acaccgaagt
     4741 cataccagca taacgtgcga gatttttcag gcgcacagcc gggagtgtac tcaggttttc
     4801 agcatgcagg ccaaaatcgt tcagagtttt ccagcgttca attgcttcat taaacgccgg
     4861 accactgatg gtcacagggc cctttttcag tgattccagt aaagacaggc ggctgcaatc
     4921 agttggcccc agcagcatct ccagctgtga acgctgttcg gctgacggta tcagtgccag
     4981 tttgttccac aggcgcaacg tcgccttttc ccttacctct gaaatcaacc gggtcagcgt
     5041 agtggctccg gggagaataa tacgatgttg cataagccac cctgtcgcca gatcgaaaag
     5101 caggccagga cgttcgttgc ttatccagct ccgggtatat aaaagacggg taaggcgaaa
     5161 tgtccagggc caggcaaatt cacgatactg atagtgctga cgtatcagcg ctgcatgctc
     5221 acggcgggta ttttccctct gaccgtattc tgcaagaacg gtgatatcac gaatcccgag
     5281 ctgtctggcg gtaaaatgcc ggacgccgga aggaatatga ttcatatcgg tgaggaaggt
     5341 gcccagaaaa cggacacatc caatttgcag ggcaatgccc agacggttgt gatcacctct
     5401 gctttttccg ataaattcct tgtctgcttc atcaaggtga aaatatcgtg ccagctgaag
     5461 ctcatccggt tcaccggtga atctgccata gctttcagtc tgctcagtgg tcagaaagtc
     5521 aacgggcata tcggcctccc tgcctgacgg gcatttagta acatttttcc aaccgtacga
     5581 aatgttataa attatcggac atcgtaaaac tgttacatta atatgtctat taaatcgtaa
     5641 atttgtaata atagacatga gttgtccgat attcgattta aggtacattt ttatgcgact
     5701 ttttggttac gctcgggtct caaccagtca gcagtctctt gatcttcagg tcagagcact
     5761 caaagacgca ggtgtgaaag caaaccgtat atttaccgat aaggcatccg gcagttcaac
     5821 agaccgggaa gggctggatt tgctgaggat gaaggtggag gaaggtgatg tcattctggt
     5881 taagaagctc gaccgtcttg gccgcgacac tgccgatatg atccaactga taaaggaatt
     5941 tgacgctcag ggcgtggcag tccggttcat tgatgacggg atcagtaccg acggtgatat
     6001 ggggcaaatg gtggtcacca tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct
     6061 agaacgcacg aatgagggcc gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag
     6121 gcgtaccgtg gacaggaacg tcgtgctgac gcttcatcag aagggcactg gtgcaacgga
     6181 aattgctcat cagctcagta ttgcccgctc cacggtttat aaaattcttg aagacgaaag
     6241 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6301 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6361 cattcaaata tgtatccgct catgagacaa taaccctggt aaatgcttca ataatattga
     6421 aaaaggaaga gtatgagtat tcaacatttt cgtgtcgccc ttattccctt ttttgcggca
     6481 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6541 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6601 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggt
     6661 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6721 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6781 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgctgc caacttactt
     6841 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6901 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6961 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7021 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7081 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7141 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7201 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7261 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7321 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7441 gaactccccg gaatggccgg agaggaaaac tgaagcatga cggggtcagg atttcttcca
     7501 gaatttccac cacggagaat gaacctgtgg agagggttcg agcttgtgtt ccagaagctg
     7561 catagcctgg cgaagagaat cgatatgttc atcacgagct ttaacagccc ctttaagacc
     7621 atcgttttca gcttcaagca aagcaatttt atgctgtaaa tcatgtaaac cagagatgtt
     7681 aacccctgta gcgcctgcgt caccttttac atcatgtttt acagtgtcgc ccccaccata
     7741 aagcgagcca aaaaccctaa taagctctga tgtatcaaca actttagtat ttttcccatg
     7801 ctttacaaca gaggtgtaac ttagtttccc actgttaatc atcttgtaaa ttgtggggcg
     7861 actaacctta acaagccgag ccgcttcact aatagacact ctggccatca gtttccatcc
     7921 tgtaattcat ccagtcccga agacagtgta cggaaaagat gctccgcatc atcgtggagt
     7981 ttctcacaaa ggtcggcctc tacatcaaga tcaagctcgt taagcttctc cagcaggagc
     8041 agagactgat tctggatgaa tttagccatg ttcagcgctg ttttctgttg tttcgtcata
     8101 agaggcctca gagcgtcagt gctcagtgta gggatgttct cttttccgct tcctcgctca
     8161 ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg caggagcatg cgtataaaag
     8221 ctcgagaaaa ccgagcgatc gcgtgatggg gagaaggaga ggccgcacag cggccgtttt
     8281 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
     8341 gaaacccgac aggactataa agataccagg cgtttccccc tggtggctcc ctcgtgcgct
     8401 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttatctc
     8461 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgt
//
