LOCUS       CP128769               13841 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain CRE-041 plasmid pCRE-041_4, complete
            sequence.
ACCESSION   CP128769
VERSION     CP128769.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878150
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 46.1307571137786x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:33:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,579
            CDSs (total)                      :: 5,455
            Genes (coding)                    :: 5,334
            CDSs (with protein)               :: 5,334
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 54 of 121
            Pseudo Genes (incomplete)         :: 71 of 121
            Pseudo Genes (internal stop)      :: 14 of 121
            Pseudo Genes (multiple problems)  :: 15 of 121
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRE-041"
                     /isolation_source="lung"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE-041_4"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.8945 N 87.6208 W"
                     /collection_date="2011-08"
     gene            44..214
                     /locus_tag="QU727_27815"
                     /pseudo=""
     CDS             44..214
                     /locus_tag="QU727_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            330..1151
                     /locus_tag="QU727_27820"
     CDS             330..1151
                     /locus_tag="QU727_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003723 - RNA binding [Evidence IEA]; GO_function:
                     GO:0016788 - hydrolase activity, acting on ester bonds
                     [Evidence IEA]; GO_function: GO:0043022 - ribosome binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WJU85172.1"
                     /translation="MSFRSTERQTPTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDK
                     AKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEY
                     GSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKD
                     AENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYG
                     DKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            1161..1418
                     /locus_tag="QU727_27825"
     CDS             1161..1418
                     /locus_tag="QU727_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WJU85173.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            1503..1652
                     /locus_tag="QU727_27830"
     CDS             1503..1652
                     /locus_tag="QU727_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WJU85174.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3133..5097
                     /locus_tag="QU727_27835"
     CDS             3133..5097
                     /locus_tag="QU727_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJU85175.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            5097..5828
                     /locus_tag="QU727_27840"
     CDS             5097..5828
                     /locus_tag="QU727_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WJU85176.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            5835..6365
                     /locus_tag="QU727_27845"
     CDS             5835..6365
                     /locus_tag="QU727_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU85177.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6393..6572)
                     /locus_tag="QU727_27850"
     CDS             complement(6393..6572)
                     /locus_tag="QU727_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJU85178.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6641..7018
                     /locus_tag="QU727_27855"
     CDS             6641..7018
                     /locus_tag="QU727_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WJU85179.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(7080..7379)
                     /locus_tag="QU727_27860"
     CDS             complement(7080..7379)
                     /locus_tag="QU727_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJU85166.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7369..7632)
                     /locus_tag="QU727_27865"
     CDS             complement(7369..7632)
                     /locus_tag="QU727_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WJU85167.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(7938..8261)
                     /locus_tag="QU727_27870"
     CDS             complement(7938..8261)
                     /locus_tag="QU727_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WJU85168.1"
                     /translation="MSFRSTERQTPFDPNFSTQKADTTRMMVPFFRQFWDMGAKDKATG
                     KSRSDVQQRIQQFHSQEFLNSLQGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYE
                     GRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(8263..11268)
                     /locus_tag="QU727_27875"
     CDS             complement(8263..11268)
                     /locus_tag="QU727_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WJU85169.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            11430..11987
                     /locus_tag="QU727_27880"
     CDS             11430..11987
                     /locus_tag="QU727_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WJU85170.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            12221..12775
                     /gene="aac(6')-Ib"
                     /locus_tag="QU727_27885"
     ARG             12222..12772
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            12845..13060
                     /locus_tag="QU727_27890"
                     /pseudo=""
     CDS             12845..13060
                     /locus_tag="QU727_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            13126..13830
                     /locus_tag="QU727_27895"
     CDS             13126..13830
                     /locus_tag="QU727_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WJU85171.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             12858..13114
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             12171..12775
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     ARG             3..214
                     /name="TEM-232"
                     /gene="TEM-232"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="100.0"
                     /coverage="24.48"
     Typing          complement(1756..2334)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5098..5825
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 aagacaaaag gctgcctcat cgctaacttt gcaacagtgc cctgataaat ctggagccgg
       61 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      121 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      181 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      241 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
      301 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      361 ctactccggc acaggtaaaa cagcgtctgg aggaagaaaa gcgtcgccag caggcatggg
      421 acgctgctca cccggaagag gggctgaaaa gagagtatga taaagcgaaa gctgagctgg
      481 atgctgaaga taaaaatatt gcgaccttaa acagtcgcat tgcatcgaca gagaaggcga
      541 tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa
      601 ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc
      661 aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa gctgcccaga
      721 catctttaaa cgaaatgaat gaatccttat ccagggataa ggctgcgctt tccggggcga
      781 tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataaa ttaaatgagg
      841 aaaagaaaaa acctcgtaag ggaactaaag attacggcca tgattatttt cctgatccca
      901 agactgaaga tattaagggg ttgggagagt tgaaagaggg taaacctaaa acccctaaac
      961 aaggtggtgg tggtaagcgg gcccgatggt atggtgataa aaagcgtaaa atttatgaat
     1021 gggattccca gcacggtgag cttgaagggt accgcgccag tgatggcgaa cacctcgggg
     1081 cattcgatcc aaaaacgggt aagcaggtta aagggccgga tccaaaacga aacattaaaa
     1141 aatatcttta agaggtaaat atggggctta aattacatat tcattggttt gataagaaaa
     1201 ccgaagagtt taaaggtggt gaatactcaa aagacttcgg tgatgatggt tctgtcattg
     1261 aaagtctggg gatgccttta aaggataata ttaataatgg ttggtttgat gttgaaaaat
     1321 catgggtttc gatattacag ccacacttta aaaatgtaat cgatattagt aaatttgatt
     1381 actttgtatc ctttgtttac cgggatggta actggtaagc aaaagttatc aaggatgatt
     1441 tgaaatacag acttccggtg ttcagggatg aataccgggg tttttaaata cagggaaggt
     1501 atatgaaaaa ggtaaaggtt gtttttcttt ttatattgat tttttcaggc tttcttctgg
     1561 tcgcatgtca ggcaaactat atccgggatg ttcagggtgg aacggtggca ccatcgtcct
     1621 cctctgaact gacggggatc gcggttcagt agaaaagatc aaaggatctt cttgagatcc
     1681 tttttttctg cgcgtaatct tttgccctgt aaacgaaaaa accacctggg gaggtggttt
     1741 gatcgaaggt taagtcagtt ggggaactgc ttaaccgtgg taactggctt tcgcagagca
     1801 cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc aagagcaacc
     1861 gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg
     1921 cgttttgcgt gcttttccgg gttggactca agtgaacagt taccggataa ggcgcagcag
     1981 tcgggctgaa cggggggttc ttgcttacag cccagcttgg agcgaacgac ctacaccgag
     2041 ccgagatacc agagtgtgag ctatgagaaa gcgccacact tcccgcaagg gagaaaggcg
     2101 gaacaggtat ccgggaaacg gcagggttgg aacaggagag cgcaagaggg agcgattcat
     2161 cggaaacggt gatgatcttt aagtcctgtc gggtttcgcc actcctgact gattcatggt
     2221 tgagccaccg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa ctcagtcagg
     2281 agggcggagc ctatggaaaa acgccacctg cgtggccttg ccgttttgcc tcacatgtta
     2341 gtcccctgct tattcacgga atctgtgggt aactttgcat gtgtccgcag cgcccgccgc
     2401 agtctcacgg ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc cccggcacct
     2461 acaagacgga caccggacgc gctgatgtgg catattgtcc cctgattaag tctttatatt
     2521 ccctgataat aaattaccag gataggtgaa tcatatcggt ttttcgctga acattttacg
     2581 ttaacctatc tctgttgctt ttttctcctt acggagaaat acaggtcatc cccgcgaggg
     2641 cgtggctgac ctcaggggga tatcccctgc ttacgctggc tggacgccac cgacgcacgg
     2701 tctctccccc tctttctggc tccgtccggc tggacgccgg ccacgccaga aattcacccg
     2761 gcccccgtca ggctcacgcc tgaccaccca caaaaatcca cggtgctgga cgcacctggc
     2821 gaagcagcaa agctgctcct gattttgcgg gtggtcagcc gctcgctatg gcgaactctg
     2881 acgggggccg gaacaccgga aaagaccgca ctggggtgcc gccttttcct ctcacacgct
     2941 ggggcgtgct cgttgttcct ccggggctgc gccttttccg catggggcgg aaagaggctg
     3001 gcctccgtgg tgaaagctga acgcaatcac cacggacgtg accgccgccg ccatgccttg
     3061 gggcatgtcc gcggcttgtg cagcgggggc tgcacatcac gccggcaaac catcgtctga
     3121 aggagagaga acatgtttaa tacggattcg cttgcctgga agtggcacat tccggagctg
     3181 gcccggcaga tttatccgtg ggtcagctgg ctgtaccagg agtattacgg tctggccggg
     3241 gctttcctag ttatcggcct gtttttcggt ctggctgaac tttgggccgg acggatgcgt
     3301 tttgtggggc tgagtcataa ggagcgacgg gagtatgccg atcgctataa gcgcgataat
     3361 aaacggccag cgccattttc ggtgctggcc tcgctggatt cgcccgggcg gatggtcagg
     3421 gcacgtcgac atgtctggaa ctacgcgtac tggctgaccg gcgcggccgg tctggccctt
     3481 ctgggggggc tggttgtcgc cgtgtttatc gagagctggg gagaaaccgt cctgcgtccg
     3541 gacctctacg caaacgcaga ttattacctg ctgcagccgg ctaacctgtt cgggattgcc
     3601 tggtgcgcgg gcgggctggc tgtcgggatt gtgttggcgc ggctgctggc gttcctgctg
     3661 cttgacggtc attttgcggc tgctgacgag gcggtgaacg cgaaactgaa tcaggagagc
     3721 cggcgcagca gccagcggac aggagagatg accgacgtgc gccacctgca tttcggggag
     3781 ccggtaccgg ttaatgcgct ggccgatttt tcctcggagc aggcccggca ggagcaggcg
     3841 gtgtttctgg gtaaggatga gcagggcaag ccggtgctgg ttccgcgtga tacctggcgc
     3901 aagaccaaca ttcagatcct cggcctgccc gggagcggca agagcgtgat ggccaccaac
     3961 gcgctcattc gctgcgtacg cgatttcggg gacgcggtgg tgtacttcga ccccaaaggg
     4021 gacgcatggg cgccgcacgt cttccgggcg cactgtccgg acttcaccct gctggatctg
     4081 cggccgggga aaccggcgca gctgaatctg ttccgcgatc tcgaccagta cgcgctgaaa
     4141 aacctgctga ttgccgggct taacctgggc gagaccggcg acgtggcgga ccactaccgc
     4201 atcagcgagc agaaagcggc gaagctgatt gcggagcagt ttccgcaggg ggcaaacatt
     4261 cagcaggtgc tggcggcggc gtacgcgctg ccggaggccc tgaaaaagga cgtgaagggg
     4321 ttgatcacta ggctggaaaa cgtcgctgac ctgagcgtcc tgcagacgga cagtggcatc
     4381 gatgtggcgg ggattatcaa cggcggcggc tgcctgtacg ttatcggctc gatggacgac
     4441 gaggcggtga tccgtgtgca aaaaatgctg tttgcccgct gcgcgcaaat catcattgcg
     4501 cgggacgagt tccgggagtg gccacacgtc agcgtgatgc tcgatgaaat taaatatctg
     4561 ctgtcgaagt atgtactgaa cgcactgggg acgctgcgca gccgggactg caatctgttg
     4621 ctcgcgcacc agtcgctggg cgatttcggg cagtgcgggc aggatctgcc cgctgaattt
     4681 gtgaaaacca cggtactgga taacacgccg atccgctggt tttaccgggc ggccagcctg
     4741 gagtccgcgc agtgggcggc ggggcagacc ggggaaatca gggtggacgt ggagcgccgc
     4801 cgtgccagcc gggaggcggg gaacgtggag catatcagcg gggacagctt catccagaag
     4861 gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc tgccggacgg gttcgcggtc
     4921 atgaccgggc tgggtgtggc ccggatggcg ttcagcagtc cgctgcgggt tgagcgccgg
     4981 gaaatcccgt tgcagcgttt cccggtgctg gcgaaaaccg atcccctggc ggagttccgg
     5041 cccccggccg acggcggcgg gccgaaagcg gatgatgatt ttgcggggat ttactgatgg
     5101 cgctggagct gtacaacgtc agccacgcga aacgacaggc gcggaacgcg gagaaaaccc
     5161 ggctgaccct gcgctggttg cgggaagagc tgtgttccac ggcggagctg gttgcgcggc
     5221 ggctggggat tgcggcggtg cagccggtct accgcttcct cgacagcctg gtggcgaaag
     5281 ggctgctggt ccggaccaaa tatccggtgg acggccgtca ggtcagcgtg tgggggctga
     5341 cgccgcatgg cgtggcgttc tcgttcgacg aggacgaacc gctggcggat gttattccgt
     5401 ttcagccgtc aagggtgtcg gcagcacatc tgccgcaccg gctggccgtg caatccctgc
     5461 gccttgatat ggaagccaga ggggccaccg gctggcgcat tctgcaccgt ctgagtctta
     5521 aaggcatgaa agtgccggac gccctggcgg agctggacgg taaaacggtg gcgtttgagg
     5581 tggagcgcac cgtcaagagc cgtcgccggt atcaggaagt ggtgtccggg tatctgttca
     5641 accgcaaagc taacggcatc gatgaaatct ggtacatctg cccggaccgg gcgacgcagg
     5701 tcagggtgca gcgggcaatc ctgtcggtgg atgaaatcgt gaacccacag accggcgagg
     5761 cccgcaaaac ggcagagctg gatcgtgaac ggttgtttgc gtgctttaaa tttatgacaa
     5821 cggagtgagg gattatggcg ctgtacatgg tatttattga agacgcggag gaccggaacg
     5881 gcgagcgctg catcatgccg accagcgagc atctggaaga tatggtggtg ctcgcctggc
     5941 agaccggggc gtatgttttc tgttttgtcg accgggccca gcatctggcg atgatgaaat
     6001 ccatcgggat gtatgacgag cggctcaaca gcgacgaaaa agccacgtac tttttcactg
     6061 gtaaaaatcc gcaatgggaa cggaacaaac ccacaggaat gaagctgtgt gattacctct
     6121 atcgcaaggt ggagcggttc gccatcgaga acggtgtgca ggtggttgat gccgggaagt
     6181 gctcaccgga gcagattaac gcgatgattg acgtggtgag tgagcggcac ggggaaagcg
     6241 cggtgaacga gaagctgaaa gcggagctga tgcgggaata taacagccgc tattttaacg
     6301 tgccggtacc tgaactgccg gaggacaaac ctgcccgccg gtggtggccg ttcggcaaaa
     6361 aataaatgcc agcaccggcc atgccggtgc tgttattcct ccccgagctt atcccgcatc
     6421 acctgccaga gtgtttccgc atcctcgtgt agccgctcgc acatgtcggc cggggtatcc
     6481 atatccagct cattcagttt atcgagcaat agcagcgact gctccttaat aaatttagcc
     6541 atattgaggg ctgttttttc ctgttttgtc atgattcttt ccttccgggc atggtgtgcg
     6601 cctgtcaggt atactgtctc aaaaatagcg gggtgagaca atggcgtggg tgacggtgag
     6661 acaggcgacg gaactgacgg gtaaagcccg aagttccctg tatcgggata tggcgaaagg
     6721 ccgcgtatca taccgtacgg aagctgatgg cggccgggtg gtggatacca gcgagcttat
     6781 ccgggtgtac ggtgagttaa gacaggatga gactcacgag cgggacgagt tgagactgtc
     6841 gggtgagact gaaaaaatgg cctccgacgc gttgatcgcg gagataaaag cactcaggga
     6901 ggaggtggcc gggctacggc aggaaatgca gtccatgaga ctgctggaac acaaaccgga
     6961 gacagcagag accccggccg gaacaccgcg ccggtggtgg ccgtggggaa aacgctgacg
     7021 cgctgtgctt gtccaaccgg cgacggccac gcaatgaatt gcgtccccgt caccggttat
     7081 tagagaacaa tatggggatt ctggcgctgg ccgaccacgg ccagaacccg gacttttctg
     7141 gtctggtcat caacatcata gaggacatgc tgtcccatca gactgatccg gcgaacgtcc
     7201 tcgccatagc gtgagtccac ttcatagagt cgcgggaagt cggccagttt gtccgccgca
     7261 gcgagaaccc ggtcggtaac gttctgcgca tcccacaggg atgaattttc cgcgatatag
     7321 tcaaacagcg tcgttacatc ctcgatggct ccctgcttcc attccacgtc atacggcatc
     7381 gcgcagttcc tgataacggc catgcaggcg ctggcggagt tgttccgtgg tcatacccgg
     7441 acgcgtgtca gcacgggcgg cggtcacttt ggcgcgaacc tgatcttcat actctgcctg
     7501 ggccagttct tcgttagcca gtcgctgcaa cagcgcagct ttgtccgggc caccttccgg
     7561 cagtatgcgg ggcaggcttt cagccagttc ccgaagaaag ttggcgtttt cgtagtgtgt
     7621 agtgttgctc attgcggagc ctcccgggtt aatgcggcaa tatgaaggaa gtttagcagg
     7681 gggcggtctg aaagaatatg gtcgtgtcag tcaggctgat gaagcagaaa gtcggtaaaa
     7741 aagggcttac ggcatccatt ttacgtcaaa acatatgcca tgcagttgcg tgcataggca
     7801 tgcattagga taaaatttat cgggcgcgtt tccggcagtt ttccgggggg gttgttgcct
     7861 gttttatccc gtagccgccg gaaacgccct gagcccgtct gagcggtgcg cgtaatgacg
     7921 cgttatggta aatccactta ctgtctgcct tcatagccat cgagataaac agctgaaatg
     7981 gtgtcagcca gtagccttga ttttttcggt gtgaggtctt tgctgcggta atcggtaccc
     8041 gcaaattgtg ttgtgccttg gagtgagttt aaaaattcct gactgtgaaa ctgctgaatg
     8101 cgttgctgca catcactccg tgattttccg gtcgctttat cctttgcgcc catatcccag
     8161 aactgccgga aaaaaggcac catcattcgt gttgtgtcag ctttctgggt ggaaaaattg
     8221 ggatcaaaag gggtctgacg ctcagtggaa cgaaaactca cgttaagcaa cgttttctgc
     8281 ctctgacgcc tcttttaatg gtctcagatg acctttggtc accagttctg ccagcgtgaa
     8341 ggaataatgg ccgagcatat tgatatgtcc gtggcaaagc ggggagaggc gtgcgatatc
     8401 ttcatcattc agtgtttcac cctgcgcccg gagatgatcc agagctgcct gcatataaat
     8461 agtgttccat aacacgacgg cgttagtgac cagccccaga gctcccaact gatcttcctg
     8521 accgtcggta tatcgttttc ttatctcacc tttttgaccg tgacagatgg ctctggcaac
     8581 tgcatgacgg ctttctcccc gattaagctg ggtcagaatg cgccggcggt aatcttcatc
     8641 atcaatataa ttaagcagat acagcgtttt gttgatgcgc cccacttcaa tgattgcctg
     8701 agtcagtccg gagggacgtt cacttttcag caatgaacgg accagcactg aggcctgtac
     8761 tttgcccagc ttcagggagc ctgcggtccg gatcatttcg tcccactgaa ggactatttt
     8821 tcggggatct gattgccctc tggcaatatc attcagcacg ccatagtcgg catcatggtc
     8881 cattcgccag aaaaccgaag caccggcatc agccaggcgt ggagaaaact ggtatcccag
     8941 cagccagaaa aggccaaaga caagatcgct ggcacctgcc gtatcggtca taatttcggt
     9001 tggattcagc ccggtctcct gttccagaag gccttccagc acaaagatag agtccctcag
     9061 cgtccccggt ataacgatgc catgaaagcc ggaatactga tcggacacaa agttgtacca
     9121 ggtgatccct ctgttattac caaagtattt gcggttcggt ccggcattga ttgttctgac
     9181 tggcgtaaca aagcgcattc catctgcaga tgccacttct cctccacccc atatctgtgc
     9241 cagtggcagc gttgcctgaa aatcaaccag tctggcatta gcgctggtga tagtttcagc
     9301 ccgcagatag ttcgcttttg tccagttcag ccggtgtcgg gtcagtgcag gaacatttga
     9361 tctgatcagt ggttccagac cgatattgca ggcttcagcc atcagcacgg cgctgatgct
     9421 gacgggcaga tcatcaactc tggcactggc ttcactggca tggaaaaact catcagcaaa
     9481 tccggtatgg gcgttaattt cgagcagcaa ctccgttaaa tccaccggag ggagcagatc
     9541 actgatcatt ttgctcagtc gtttcagact gtccggctca tcaagactgg cgaggggaga
     9601 aattgtcaac cggggcttcg ggccagaaac atcgagttcg acagcctcat tttcgccaag
     9661 acgtgcagca acctgtctgt aacgactatc aagctgatgg cccagagatt ttattgcttc
     9721 ctgcgggtct gtcgggtgcc ccaaagaacg ataaacctta atccgatttg cctgccagtc
     9781 agcaccctgt agtaatcttg cacgaggatc tccccaccgg ttactgccgg taacgtagac
     9841 atccctccgt ctcagactat cctgcagttt actgagaaag cagagcgtgt atccccttcg
     9901 ggtgatatgt ttttccttgt taatcaccag ccgtttccat gactgactga tgatttccgt
     9961 tggtgcgtcg tcaaaaaact gccgccgtga gctgaactcc cggctgaggt agtcacaggc
    10021 attcagagtg gtaaccccgg caggtgcgga tgaaaattta acggtattca gcagatgggg
    10081 caggaaacga cgaacgcgcc cgtactgctc caccatttcg tcatgaaaat tatcgtctga
    10141 gggccgggca atttcacgga caagcgtgat gatttcagcc agcttttgcc tagggatgta
    10201 gctgaacacc tcagcacgaa tcgattcgtc cggtgtttct tctttcagca agtacgaaca
    10261 tgcgctggcg agcgccaatg cagatttatc cagatccttc agcgagcgga gccgtttttt
    10321 ctgcccaatc tttctggcgt cacggatgat aacggccagc atggcgtcca gaacgtccag
    10381 tgcatcatcc agcgccagcg tttcccatgc aaggacaaag gcaaccagaa ccgccatcct
    10441 tttctgcggt gacatcctgg caatattgaa caccgaagtc ataccagcat aacgtgcgag
    10501 atttttcagg cgcacagccg ggagtgtact caggttttca gcatgcaggc caaaatcgtt
    10561 cagagttttc cagcgttcaa ttgcttcatt aaacgccgga ccactgatgg tcacagggcc
    10621 ttttttcagt gattccagta aagacaggcg gctgcaatca gttggcccca gcagcatctc
    10681 cagctgtgaa cgctgttcgg ctgacggtat cagtgccagt ttgttccaca ggcgcaacgt
    10741 cgccttttcc cttacctctg aaatcaaccg ggtcagcgtg gtggctccgg ggagaataat
    10801 acgatgttgc ataagccacc ctgtcgccag gtcgaaaagc aggccaggac gttcgttgct
    10861 tatccagctc cgggtatata aaagacgggt aaggcgaaat gtccagggcc aggcaaattc
    10921 acgatactga tagtgctgac gtatcagcgc tgcatgctca cggcgggtat tttccctctg
    10981 accgtattct gcaagaacgg tgatatcacg aatcccgagc tgtctggcgg taaaatgccg
    11041 gacgccggaa ggaatatgat tcatgtcagt aagaaaagtg cccagaaaac gcacacaccc
    11101 gatttgcagg gcaataccaa gtcgattgtg atcaccccgg cttttcccga taaattcttt
    11161 atccgcttca tcaagatgaa aataacgcgc cagctgaagt tcatcgggtt cgccagtgaa
    11221 cctgccataa ctctcaacct gctcagtggt caaaaaatca acgggcatat cggcctccct
    11281 gcctgacggc ttttttaaca caactgcaac cgttcgaaat attataaatt atcagacata
    11341 gtaaaacggc ttcgtttgag tgtccattaa atcgtcattt tggcataata gacacatcgt
    11401 gtctgatatt cgatttaagg tacattttta tgcgaatttt tggttatgcg cgggtctcaa
    11461 ccagccagca gtccctcgat attcagatca gagcgctcaa agatgcaggg gtaaaagcta
    11521 accgcatctt taccgacaag gcatccggca gttcaacaga tcgggaaggg ctggatttgc
    11581 tgaggatgaa ggtggaggaa ggtgatgtca ttctggtgaa gaagctcgac cgtcttggcc
    11641 gcgacaccgc cgacatgatc caactgataa aagagtttga tgctcagggt gtagcggttc
    11701 ggtttattga cgacgggatc agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc
    11761 tgtcggctgt ggcacaggct gaacgccgga ggatcctaga gcgcacgaat gagggccgac
    11821 aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg taccgtggac aggaacgtcg
    11881 tgctgacgct tcatcagaag ggcactggtg caacggaaat tgctcatcag ctcagtattg
    11941 cccgctccac ggtttataaa attcttgaag acgaaagggc ctcgtgatac gcctattttt
    12001 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
    12061 agtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    12121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    12181 acatttccaa acaaagttag gcatcacaaa gtacagcatc gtgaccaaca gcaacgattc
    12241 cgtcacactg cgcctcatga ctgagcatga ccttgcgatg ctctatgagt ggctaaatcg
    12301 atctcatatc gtcgagtggt ggggcggaga agaagcacgc ccgacacttg ctgacgtaca
    12361 ggaacagtac ttgccaagcg ttttagcgca agagtccgtc actccataca ttgcaatgct
    12421 gaatggagag ccgattgggt atgcccagtc gtacgttgct cttggaagcg gggacggatg
    12481 gtgggaagaa gaaaccgatc caggagtacg cggaatagac cagttactgg cgaatgcatc
    12541 acaactgggc aaaggcttgg gaaccaagct ggttcgagct ctggttgagt tgctgttcaa
    12601 tgatcccgag gtcaccaaga tccaaacgga cccgtcgccg agcaacttgc gagcgatccg
    12661 atgctacgag aaagcggggt ttgagaggca aggtaccgta accaccccag atggtccagc
    12721 cgtgtacatg gttcaaacac gccaggcatt cgagcgaaca cgcagtgttg cctaaccctt
    12781 ccatcgaggg ggacgtccaa gggctggcgc ccttggccgc ccctcatgtc aaacgttaaa
    12841 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
    12901 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
    12961 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
    13021 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acggcactgt tgcaaatagt
    13081 cggtggtgat aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa
    13141 ggccggcatt ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc
    13201 atcagttacc gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc
    13261 acgatttacc gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac
    13321 tggcgtaacc cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat
    13381 ggccgctggg cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat
    13441 ctctcctccc gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaac
    13501 gtgaagaagt ggcagatccc gcgattcatc aacacggata aagcgcccgc ctatggtcgc
    13561 gcgcttgctc tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt
    13621 aagtaccgga acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc
    13681 acgctgggat ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg
    13741 cgtgcactac gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg
    13801 cgcctggtaa gcagagtttt tgaaatgtaa ggcctttgaa t
//
