LOCUS       CP128750                9294 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain CRE-229 plasmid pCRE-229_7, complete
            sequence.
ACCESSION   CP128750
VERSION     CP128750.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878154
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83.8215170064267x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 14:10:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,338
            CDSs (total)                      :: 6,215
            Genes (coding)                    :: 6,015
            CDSs (with protein)               :: 6,015
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 93 of 200
            Pseudo Genes (incomplete)         :: 116 of 200
            Pseudo Genes (internal stop)      :: 18 of 200
            Pseudo Genes (multiple problems)  :: 25 of 200
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRE-229"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE-229_7"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.8945 N 87.6208 W"
                     /collection_date="2015-09"
     gene            join(9244..9294,1..249)
                     /locus_tag="QU724_31610"
     CDS             join(9244..9294,1..249)
                     /locus_tag="QU724_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJU79835.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(311..688)
                     /locus_tag="QU724_31615"
     CDS             complement(311..688)
                     /locus_tag="QU724_31615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WJU79836.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            757..936
                     /locus_tag="QU724_31620"
     CDS             757..936
                     /locus_tag="QU724_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJU79837.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(964..1494)
                     /locus_tag="QU724_31625"
     CDS             complement(964..1494)
                     /locus_tag="QU724_31625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU79838.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1501..2232)
                     /locus_tag="QU724_31630"
     CDS             complement(1501..2232)
                     /locus_tag="QU724_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WJU79839.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(2232..4196)
                     /locus_tag="QU724_31635"
     CDS             complement(2232..4196)
                     /locus_tag="QU724_31635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJU79840.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5677..5826)
                     /locus_tag="QU724_31640"
     CDS             complement(5677..5826)
                     /locus_tag="QU724_31640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WJU79830.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5911..6168)
                     /locus_tag="QU724_31645"
     CDS             complement(5911..6168)
                     /locus_tag="QU724_31645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WJU79831.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(6178..7863)
                     /locus_tag="QU724_31650"
     CDS             complement(6178..7863)
                     /locus_tag="QU724_31650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WJU79832.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8266..8685
                     /locus_tag="QU724_31655"
     CDS             8266..8685
                     /locus_tag="QU724_31655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WJU79833.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8991..9254
                     /locus_tag="QU724_31660"
     CDS             8991..9254
                     /locus_tag="QU724_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WJU79834.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     BGC             1178..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4996..5574
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1505..2232)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ctgtttgact atatcgcgga aaattcatcc ctgtgggatg cgcagaacgt taccgaccgg
       61 gttctcgctg cggcggacaa actggccgac ttcccgcgac tctatgaagt ggactcacgc
      121 tatggcgagg acgttcgccg gatcagtctg atgggacagc atgtcctcta tgatgttgat
      181 gaccagacca gaaaagtccg ggttctggcc gtggtcggcc agcgccagaa tccccatatt
      241 gttctctaat aaccggtgac ggggacgcaa ttcattgcgt ggccgtcgcc ggttggacaa
      301 gcacagcgcg tcagcgtttt ccccacggcc accaccggcg cggtgttccg gccggggtct
      361 ctgctgtctc cggtttgtgt tccagcagtc tcatggactg catttcctgc cgtagcccgg
      421 ccacctcctc cctgagtgct tttatctccg cgatcaacgc gtcggaggcc attttttcag
      481 tctcacccga cagtctcaac tcgtcccgct cgtgagtctc atcctgtctt aactcaccgt
      541 acacccggat aagctcgctg gtatccacca cccggccgcc atcagcttcc gtacggtatg
      601 atacgcggcc tttcgccata tcccgataca gggaacttcg ggctttaccc gtcagttccg
      661 tcgcctgtct caccgtcacc cacgccattg tctcaccccg ctatttttga gacagtatac
      721 ctgacaggcg cacaccatgc ccggaaggaa agaatcatga caaaacagga aaaaacagcc
      781 ctcaatatgg ctaaatttat taaggagcag tcgctgctat tgctcgataa actgaatgag
      841 ctggatatgg ataccccggc cgacatgtgc gagcggctac acgaggatgc ggaaacactc
      901 tggcaggtga tgcgggataa gctcggggag gaataacagc accggcatgg ccggtgctgg
      961 catttatttt ttgccgaacg gccaccaccg gcgggcaggt ttgtcctccg gcagttcagg
     1021 taccggcacg ttaaaatagc ggctgttata ttcccgcatc agctccgctt tcagcttctc
     1081 gttcaccgcg ctttccccgt gccgctcact caccacgtca atcatcgcgt taatctgctc
     1141 cggtgagcac ttcccggcat caaccacctg cacaccgttc tcgatggcga accgctccac
     1201 cttgcgatag aggtaatcac acagcttcat tcctgtgggt ttgttccgtt cccattgcgg
     1261 atttttacca gtgaaaaagt acgtggcttt ttcgtcgctg ttgagccgct cgtcatacat
     1321 cccgatggat ttcatcatcg ccagatgctg ggcccggtcg acaaaacaga aaacatacgc
     1381 cccggtctgc caggcgagca ccaccatatc ttccagatgc tcgctggtcg gcatgatgca
     1441 gcgctcgccg ttccggtcct ccgcgtcttc aataaatacc atgtacagcg ccataatccc
     1501 tcactccgtt gtcataaatt taaagcacgc aaacaaccgt tcacgatcca gctctgccgt
     1561 tttgcgggcc tcgccggtct gtgggttcac gatttcatcc accgacagga ttgcccgctg
     1621 caccctgacc tgcgtcgccc ggtccgggca gatgtaccag atttcatcga tgccgttagc
     1681 tttgcggttg aacagatacc cggacaccac ttcctgatac cggcgacggc tcttgacggt
     1741 gcgctccacc tcaaacgcca ccgttttacc gtccagctcc gccagggcgt ccggcacttt
     1801 catgccttta agactcagac ggtgcagaat gcgccagccg gtggcccctc tggcttccat
     1861 atcaaggcgc agggattgca cggccagccg gtgcggcaga tgtgctgccg acacccttga
     1921 cggctgaaac ggaataacat ccgccagcgg ttcgtcctcg tcgaacgaga acgccacgcc
     1981 atgcggcgtc agcccccaca cgctgacctg acggccgtcc accggatatt tggtccggac
     2041 cagcagccct ttcgccacca ggctgtcgag gaagcggtag accggctgca ccgccgcaat
     2101 ccccagccgc cgcgcaacca gctccgccgt ggaacacagc tcttcccgca accagcgcag
     2161 ggtcagccgg gttttctccg cgttccgcgc ctgtcgtttc gcgtggctga cgttgtacag
     2221 ctccagcgcc atcagtaaat ccccgcaaaa tcatcatccg ctttcggccc gccgccgtcg
     2281 gccgggggcc ggaactccgc caggggatcg gttttcgcca gcaccgggaa acgctgcaac
     2341 gggatttccc ggcgctcaac ccgcagcgga ctgctgaacg ccatccgggc cacacccagc
     2401 ccggtcatga ccgcgaaccc gtccggcagg tgttgcaggg tgttcacgtc aaacagcggc
     2461 cgcgcctcct tctggatgaa gctgtccccg ctgatatgct ccacgttccc cgcctcccgg
     2521 ctggcacggc ggcgctccac gtccaccctg atttccccgg tctgccccgc cgcccactgc
     2581 gcggactcca ggctggccgc ccggtaaaac cagcggatcg gcgtgttatc cagtaccgtg
     2641 gttttcacaa attcagcggg cagatcctgc ccgcactgcc cgaaatcgcc cagcgactgg
     2701 tgcgcgagca acagattgca gtcccggctg cgcagcgtcc ccagtgcgtt cagtacatac
     2761 ttcgacagca gatatttaat ttcatcgagc atcacgctga cgtgtggcca ctcccggaac
     2821 tcgtcccgcg caatgatgat ttgcgcgcag cgggcaaaca gcattttttg cacacggatc
     2881 accgcctcgt cgtccatcga gccgataacg tacaggcagc cgccgccgtt gataatcccc
     2941 gccacatcga tgccactgtc cgtctgcagg acgctcaggt cagcgacgtt ttccagccta
     3001 gtgatcaacc ccttcacgtc ctttttcagg gcctccggca gcgcgtacgc cgccgccagc
     3061 acctgctgaa tgtttgcccc ctgcggaaac tgctccgcaa tcagcttcgc cgctttctgc
     3121 tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcgc ccaggttaag cccggcaatc
     3181 agcaggtttt tcagcgcgta ctggtcgaga tcgcggaaca gattcagctg cgccggtttc
     3241 cccggccgca gatccagcag ggtgaagtcc ggacagtgcg cccggaagac gtgcggcgcc
     3301 catgcgtccc ctttggggtc gaagtacacc accgcgtccc cgaaatcgcg tacgcagcga
     3361 atgagcgcgt tggtggccat cacgctcttg ccgctcccgg gcaggccgag gatctgaatg
     3421 ttggtcttgc gccaggtatc acgcggaacc agcaccggct tgccctgctc atccttaccc
     3481 agaaacaccg cctgctcctg ccgggcctgc tccgaggaaa aatcggccag cgcattaacc
     3541 ggtaccggct ccccgaaatg caggtggcgc acgtcggtca tctctcctgt ccgctggctg
     3601 ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcagc cgcaaaatga
     3661 ccgtcaagca gcaggaacgc cagcagccgc gccaacacaa tcccgacagc cagcccgccc
     3721 gcgcaccagg caatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgtttgcg
     3781 tagaggtccg gacgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc
     3841 ccccccagaa gggccagacc ggccgcgccg gtcagccagt acgcgtagtt ccagacatgt
     3901 cgacgtgccc tgaccatccg cccgggcgaa tccagcgagg ccagcaccga aaatggcgct
     3961 ggccgtttat tatcgcgctt atagcgatcg gcatactccc gtcgctcctt atgactcagc
     4021 cccacaaaac gcatccgtcc ggcccaaagt tcagccagac cgaaaaacag gccgataact
     4081 aggaaagccc cggccagacc gtaatactcc tggtacagcc agctgaccca cggataaatc
     4141 tgccgggcca gctccggaat gtgccacttc caggcaagcg aatccgtatt aaacatgttc
     4201 tctctccttc agacgatggt ttgccggcgt gatgtgcagc ccccgctgca caagccgcgg
     4261 acatgcccca aggcatggcg gcggcggtca cgtccgtggt gattgcgttc agctttcacc
     4321 acggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag gaacaacgag
     4381 cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc cggtgttccg
     4441 gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt
     4501 gctgcttcgc caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg
     4561 acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacggagc cagaaagagg
     4621 gggagagacc gtgcgtcggt ggcgtccagc cagcgtaagc aggggatatc cccctgaggt
     4681 cagccacgcc ctcgcgggga tgacctgtat ttctccgtaa ggagaaaaaa gcaacagaga
     4741 taggttaacg taaaatgttc agcgaaaaac cgatatgatt cacctatcct ggtaatttat
     4801 tatcagggaa tataaagact taatcagggg acaatatgcc acatcagcgc gtccggtgtc
     4861 cgtcttgtag gtgccgggga aattgcgcct cctcgctcac tcggtcgcta cgctccggcc
     4921 gtgagactgc ggcgggcgct gcggacacat gcaaagttac ccacagattc cgtgaataag
     4981 caggggacta acatgtgagg caaaacggca aggccacgca ggtggcgttt ttccataggc
     5041 tccgccctcc tgactgagtt cacataaaca gacgcttttt cggtgcatct gtgggagccg
     5101 gtggctcaac catgaatcag tcaggagtgg cgaaacccga caggacttaa agatcatcac
     5161 cgtttccgat gaatcgctcc ctcttgcgct ctcctgttcc aaccctgccg tttcccggat
     5221 acctgttccg cctttctccc ttgcgggaag tgtggcgctt tctcatagct cacactctgg
     5281 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga accccccgtt
     5341 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc ggaaaagcac
     5401 gcaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct
     5461 aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt
     5521 ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac
     5581 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
     5641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     5701 ttcagaggag gacgatggtg ccaccgttcc accctgaaca tcccggatat agtttgcctg
     5761 acatgcgacc agaagaaagc ctgaaaaaat caatataaaa agaaaaacaa cctttacctt
     5821 tttcatatac cttccctgta tttaaaaacc ccggtattca tccctgaaca ccggaagtct
     5881 gtatttcaaa tcatccttga taacttttgc ttaccagtta ccatcccggt aaacaaagga
     5941 tacaaagtaa tcaaatttac taatatcgat tacattttta aagtgtggct gtaatatcga
     6001 aacccatgat ttttcaacat caaaccaacc attattaata ttatccttta aaggcatccc
     6061 cagactttca atgacagaac catcatcacc gaagtctttt gagtattcac cacctttaaa
     6121 ctcttcggtt ttcttatcaa accaatgaat atgtaattta agccccatat ttacctctta
     6181 aagatatttt ttaatgtttc gttttggatc cggcccttta acctgcttac ccgtttttgg
     6241 atcgaatgcc ccgaggtgtt cgccatcact ggcgcggtac ccttcaagct caccgtgctg
     6301 ggaatcccat tcataaattt tacgcttttt atcaccatac catcgggccc gcttaccacc
     6361 accaccttgt ttaggggttt taggtttacc ctctttcaac tctcccaacc ccttaatatc
     6421 ttcagtcttg ggatcaggaa aataatcatg gccgtaatct ttagttccct tacgaggttt
     6481 tttcttttcc tcatttaatt tattttctgc atccttcgct tttttctcct tttgtttccg
     6541 gctctccatc gccccggaaa gcgcagcctt atccctggat aaggattcat tcatttcgtt
     6601 taaagatgtc tgggcagctt ttaatttctc attctgattc tgaatatcct tatcaagata
     6661 gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag tcacaaaatc
     6721 atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag cagcccttgc
     6781 accggggatc gccttctctg tcgatgcaat gcgactgttt aaggtcgcaa tatttttatc
     6841 ttcagcatcc agctcagctt tcgctttatc atactctctt ttcagcccct cttccgggtg
     6901 agcagcgtcc catgcctgct ggcgacgctt ttcttcctcc agacgctgtt ttacctgtgc
     6961 cggagtaaga acatccgtca ccgacacata gaccggtttc tgtccgctct ctttcgggaa
     7021 acgaataacg gcttcacggg agttaccacc ggccgtaaaa ccagccggac gtgaatcacc
     7081 tttttcagca ataatgcctt ttggcggggc tttcgcggcc ggaacacctt taggtacgct
     7141 cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac gttttgtcgg
     7201 tttcgcatcc accacaggaa cactcattgg ccggccggtg acaaccgcaa tatgctgccg
     7261 ctcgtccttc acaatatccg ccacacgttt agtgacgctg accgtcgcct ggtccagcgg
     7321 cagggagctg accggcgtct ccgttaccgt gtctgccggc aatgacgtca cgatttttga
     7381 catcattttc ggatcatctt ttgctatctc agaaggcagc actccgtaaa tcgcaatccc
     7441 ccacagacca aacttaaacg gacctttcag ggcagccagc acatcagcaa cagcggccga
     7501 caacgcatcg ccggaaacgg ataaagccag tccttcagcc cccggagtgg ccaaggcagg
     7561 cagaccgaag gccatcgcgg tggccgaaga ctgtccgcca ccagagctcc cgctatgatt
     7621 gccgctgtta ccgccattac caccgggttt actcccgccg ttaccaaact cccccgggtt
     7681 atagttatga atatccccgt taggtgtatg cgtcgtaccc cagcccggcc cgctgtttgg
     7741 atccgtacca ccacctgatg aagggccacc tttacctgat gtaccgttca cattcccact
     7801 ggcctgacca ccattgtgtc ccagacctga attacccgga cctcgtccgt ctccgccact
     7861 catatacttt tcctctttga cttttaaaac aataaactaa aataaaatac tgtacatata
     7921 accactggtt ttatatacag tataaaaatt acgctgcctt gtttttcatg tcaagtctgt
     7981 aaatttgcgt ttttgtgaaa atgatcaaaa aaacagccac tcacaccatg cgcaatcccc
     8041 tccagatgca acaaaaacca gcacaaacgg cgccgttctc cggcctcagt atgttttact
     8101 gaaatcatta tatgagatac cagaaagccc cctccgggac aggaaaagaa acggggggtc
     8161 tggttcgggt tgttcggtca ctgaacacgg tcagaaactg atgataagat agcctgcgga
     8221 tatataactc aatgagaaaa aagaattaaa ggtaaggaga cgctcatgag acgttccggc
     8281 agcaaactgt gtcttatcgc catgataaca gcgacagcaa ctctcactgc ctgtgctcca
     8341 aagggcagcg tggaacagca tacccggcat tacgtatatg cttcagatga cggttttgat
     8401 cccaattttt ccacccagaa agctgacaca acacgaatga tggtgccttt tttccggcag
     8461 ttctgggata tgggcgcaaa ggataaagcg accggaaaat cacggagtga tgtgcagcaa
     8521 cgcattcagc agtttcacag tcaggaattt ttaaactcac tccaaggcac aacacaattt
     8581 gcgggtaccg attaccgcag caaagacctc acaccgaaaa aatcaaggct actggctgac
     8641 accatttcag ctgtttatct cgatggctat gaaggcagac agtaagtgga tttaccataa
     8701 cgcgtcatta cgcgcaccgc tcagacgggc tcagggcgtt tccggcggct acgggataaa
     8761 acaggcaaca acccccccgg aaaactgccg gaaacgcgcc cgataaattt tatcctaatg
     8821 catgcctatg cacgcaactg catggcatat gttttgacgt aaaatggatg ccgtaagccc
     8881 ttttttaccg actttctgct tcatcagcct gactgacacg accatattct ttcagaccgc
     8941 cccctgctaa acttccttca tattgccgca ttaacccggg aggctccgca atgagcaaca
     9001 ctacacacta cgaaaacgcc aactttcttc gggaactggc tgaaagcctg ccccgcatac
     9061 tgccggaagg tggcccggac aaagctgcgc tgttgcagcg actggctaac gaagaactgg
     9121 cccaggcaga gtatgaagat caggttcgcg ccaaagtgac cgccgcccgt gctgacacgc
     9181 gtccgggtat gaccacggaa caactccgcc agcgcctgca tggccgttat caggaactgc
     9241 gcgatgccgt atgacgtgga atggaagcag ggagccatcg aggatgtaac gacg
//
