LOCUS       CP128741                9294 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain CRE-231 plasmid pCRE-231_5, complete
            sequence.
ACCESSION   CP128741
VERSION     CP128741.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878155
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 55.5478900268573x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 14:00:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,219
            CDSs (total)                      :: 6,092
            Genes (coding)                    :: 5,927
            CDSs (with protein)               :: 5,927
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 61 of 165
            Pseudo Genes (incomplete)         :: 104 of 165
            Pseudo Genes (internal stop)      :: 26 of 165
            Pseudo Genes (multiple problems)  :: 23 of 165
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRE-231"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE-231_5"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.8945 N 87.6208 W"
                     /collection_date="2015-09"
     gene            complement(437..586)
                     /locus_tag="QU719_31015"
     CDS             complement(437..586)
                     /locus_tag="QU719_31015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WJU91100.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(671..928)
                     /locus_tag="QU719_31020"
     CDS             complement(671..928)
                     /locus_tag="QU719_31020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WJU91101.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(938..2623)
                     /locus_tag="QU719_31025"
     CDS             complement(938..2623)
                     /locus_tag="QU719_31025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WJU91102.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3026..3445
                     /locus_tag="QU719_31030"
     CDS             3026..3445
                     /locus_tag="QU719_31030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WJU91103.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3751..4014
                     /locus_tag="QU719_31035"
     CDS             3751..4014
                     /locus_tag="QU719_31035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WJU91104.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            4004..4303
                     /locus_tag="QU719_31040"
     CDS             4004..4303
                     /locus_tag="QU719_31040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJU91105.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4365..4742)
                     /locus_tag="QU719_31045"
     CDS             complement(4365..4742)
                     /locus_tag="QU719_31045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WJU91106.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            4811..4990
                     /locus_tag="QU719_31050"
     CDS             4811..4990
                     /locus_tag="QU719_31050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJU91096.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5018..5548)
                     /locus_tag="QU719_31055"
     CDS             complement(5018..5548)
                     /locus_tag="QU719_31055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU91097.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5555..6286)
                     /locus_tag="QU719_31060"
     CDS             complement(5555..6286)
                     /locus_tag="QU719_31060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WJU91098.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(6286..8250)
                     /locus_tag="QU719_31065"
     CDS             complement(6286..8250)
                     /locus_tag="QU719_31065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJU91099.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7623
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          45..173
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.83e-61"
     Typing          9050..9294
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.093"
                     /coverage="100.0"
                     /evalue="1.61e-88"
     Typing          complement(5559..6286)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ttgcgggaag tgtggcgctt tctcatagct cacactctgg tatctcggct cggtgtaggt
       61 cgttcgctcc aagctgggct gtaagcaaga accccccgtt cagcccgact gctgcgcctt
      121 atccggtaac tgttcacttg agtccaaccc ggaaaagcac gcaaaacgcc actggcagca
      181 gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga
      241 agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa
      301 gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc
      361 caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa
      421 gatcctttga tcttttctac tgaaccgcga tccccgtcag ttcagaggag gacgatggtg
      481 ccaccgttcc accctgaaca tcccggatat agtttgcctg acatgcgacc agaagaaagc
      541 ctgaaaaaat caatataaaa agaaaaacaa cctttacctt tttcatatac cttccctgta
      601 tttaaaaacc ccggtattca tccctgaaca ccggaagtct gtatttcaaa tcatccttga
      661 taacttttgc ttaccagtta ccatcccggt aaacaaagga tacaaagtaa tcaaatttac
      721 taatatcgat tacattttta aagtgtggct gtaatatcga aacccatgat ttttcaacat
      781 caaaccaacc attattaata ttatccttta aaggcatccc cagactttca atgacagaac
      841 catcatcacc gaagtctttt gagtattcac cacctttaaa ctcttcggtt ttcttatcaa
      901 accaatgaat atgtaattta agccccatat ttacctctta aagatatttt ttaatgtttc
      961 gttttggatc cggcccttta acctgcttac ccgtttttgg atcgaatgcc ccgaggtgtt
     1021 cgccatcact ggcgcggtac ccttcaagct caccgtgctg ggaatcccat tcataaattt
     1081 tacgcttttt atcaccatac catcgggccc gcttaccacc accaccttgt ttaggggttt
     1141 taggtttacc ctctttcaac tctcccaacc ccttaatatc ttcagtcttg ggatcaggaa
     1201 aataatcatg gccgtaatct ttagttccct tacgaggttt tttcttttcc tcatttaatt
     1261 tattttctgc atccttcgct tttttctcct tttgtttccg gctctccatc gccccggaaa
     1321 gcgcagcctt atccctggat aaggattcat tcatttcgtt taaagatgtc tgggcagctt
     1381 ttaatttctc attctgattc tgaatatcct tatcaagata gcgaacctga tcctgccacc
     1441 cggagccata ttcatgaggc gggttataag tcacaaaatc atctttattt gcctctgcct
     1501 cttttacctt tttatcggct tcctgaacag cagcccttgc accggggatc gccttctctg
     1561 tcgatgcaat gcgactgttt aaggtcgcaa tatttttatc ttcagcatcc agctcagctt
     1621 tcgctttatc atactctctt ttcagcccct cttccgggtg agcagcgtcc catgcctgct
     1681 ggcgacgctt ttcttcctcc agacgctgtt ttacctgtgc cggagtaaga acatccgtca
     1741 ccgacacata gaccggtttc tgtccgctct ctttcgggaa acgaataacg gcttcacggg
     1801 agttaccacc ggccgtaaaa ccagccggac gtgaatcacc tttttcagca ataatgcctt
     1861 ttggcggggc tttcgcggcc ggaacacctt taggtacgct cacctgcaga gacgggagac
     1921 ccggaatcga cacactgaat acccccggac gttttgtcgg tttcgcatcc accacaggaa
     1981 cactcattgg ccggccggtg acaaccgcaa tatgctgccg ctcgtccttc acaatatccg
     2041 ccacacgttt agtgacgctg accgtcgcct ggtccagcgg cagggagctg accggcgtct
     2101 ccgttaccgt gtctgccggc aatgacgtca cgatttttga catcattttc ggatcatctt
     2161 ttgctatctc agaaggcagc actccgtaaa tcgcaatccc ccacagacca aacttaaacg
     2221 gacctttcag ggcagccagc acatcagcaa cagcggccga caacgcatcg ccggaaacgg
     2281 ataaagccag tccttcagcc cccggagtgg ccaaggcagg cagaccgaag gccatcgcgg
     2341 tggccgaaga ctgtccgcca ccagagctcc cgctatgatt gccgctgtta ccgccattac
     2401 caccgggttt actcccgccg ttaccaaact cccccgggtt atagttatga atatccccgt
     2461 taggtgtatg cgtcgtaccc cagcccggcc cgctgtttgg atccgtacca ccacctgatg
     2521 aagggccacc tttacctgat gtaccgttca cattcccact ggcctgacca ccattgtgtc
     2581 ccagacctga attacccgga cctcgtccgt ctccgccact catatacttt tcctctttga
     2641 cttttaaaac aataaactaa aataaaatac tgtacatata accactggtt ttatatacag
     2701 tataaaaatt acgctgcctt gtttttcatg tcaagtctgt aaatttgcgt ttttgtgaaa
     2761 atgatcaaaa aaacagccac tcacaccatg cgcaatcccc tccagatgca acaaaaacca
     2821 gcacaaacgg cgccgttctc cggcctcagt atgttttact gaaatcatta tatgagatac
     2881 cagaaagccc cctccgggac aggaaaagaa acggggggtc tggttcgggt tgttcggtca
     2941 ctgaacacgg tcagaaactg atgataagat agcctgcgga tatataactc aatgagaaaa
     3001 aagaattaaa ggtaaggaga cgctcatgag acgttccggc agcaaactgt gtcttatcgc
     3061 catgataaca gcgacagcaa ctctcactgc ctgtgctcca aagggcagcg tggaacagca
     3121 tacccggcat tacgtatatg cttcagatga cggttttgat cccaattttt ccacccagaa
     3181 agctgacaca acacgaatga tggtgccttt tttccggcag ttctgggata tgggcgcaaa
     3241 ggataaagcg accggaaaat cacggagtga tgtgcagcaa cgcattcagc agtttcacag
     3301 tcaggaattt ttaaactcac tccaaggcac aacacaattt gcgggtaccg attaccgcag
     3361 caaagacctc acaccgaaaa aatcaaggct actggctgac accatttcag ctgtttatct
     3421 cgatggctat gaaggcagac agtaagtgga tttaccataa cgcgtcatta cgcgcaccgc
     3481 tcagacgggc tcagggcgtt tccggcggct acgggataaa acaggcaaca acccccccgg
     3541 aaaactgccg gaaacgcgcc cgataaattt tatcctaatg catgcctatg cacgcaactg
     3601 catggcatat gttttgacgt aaaatggatg ccgtaagccc ttttttaccg actttctgct
     3661 tcatcagcct gactgacacg accatattct ttcagaccgc cccctgctaa acttccttca
     3721 tattgccgca ttaacccggg aggctccgca atgagcaaca ctacacacta cgaaaacgcc
     3781 aactttcttc gggaactggc tgaaagcctg ccccgcatac tgccggaagg tggcccggac
     3841 aaagctgcgc tgttgcagcg actggctaac gaagaactgg cccaggcaga gtatgaagat
     3901 caggttcgcg ccaaagtgac cgccgcccgt gctgacacgc gtccgggtat gaccacggaa
     3961 caactccgcc agcgcctgca tggccgttat caggaactgc gcgatgccgt atgacgtgga
     4021 atggaagcag ggagccatcg aggatgtaac gacgctgttt gactatatcg cggaaaattc
     4081 atccctgtgg gatgcgcaga acgttaccga ccgggttctc gctgcggcgg acaaactggc
     4141 cgacttcccg cgactctatg aagtggactc acgctatggc gaggacgttc gccggatcag
     4201 tctgatggga cagcatgtcc tctatgatgt tgatgaccag accagaaaag tccgggttct
     4261 ggccgtggtc ggccagcgcc agaatcccca tattgttctc taataaccgg tgacggggac
     4321 gcaattcatt gcgtggccgt cgccggttgg acaagcacag cgcgtcagcg ttttccccac
     4381 ggccaccacc ggcgcggtgt tccggccggg gtctctgctg tctccggttt gtgttccagc
     4441 agtctcatgg actgcatttc ctgccgtagc ccggccacct cctccctgag tgcttttatc
     4501 tccgcgatca acgcgtcgga ggccattttt tcagtctcac ccgacagtct caactcgtcc
     4561 cgctcgtgag tctcatcctg tcttaactca ccgtacaccc ggataagctc gctggtatcc
     4621 accacccggc cgccatcagc ttccgtacgg tatgatacgc ggcctttcgc catatcccga
     4681 tacagggaac ttcgggcttt acccgtcagt tccgtcgcct gtctcaccgt cacccacgcc
     4741 attgtctcac cccgctattt ttgagacagt atacctgaca ggcgcacacc atgcccggaa
     4801 ggaaagaatc atgacaaaac aggaaaaaac agccctcaat atggctaaat ttattaagga
     4861 gcagtcgctg ctattgctcg ataaactgaa tgagctggat atggataccc cggccgacat
     4921 gtgcgagcgg ctacacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg
     4981 ggaggaataa cagcaccggc atggccggtg ctggcattta ttttttgccg aacggccacc
     5041 accggcgggc aggtttgtcc tccggcagtt caggtaccgg cacgttaaaa tagcggctgt
     5101 tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct
     5161 cactcaccac gtcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca
     5221 cctgcacacc gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa tcacacagct
     5281 tcattcctgt gggtttgttc cgttcccatt gcggattttt accagtgaaa aagtacgtgg
     5341 ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat
     5401 gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca
     5461 tatcttccag atgctcgctg gtcggcatga tgcagcgctc gccgttccgg tcctccgcgt
     5521 cttcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc
     5581 acgcaaacaa ccgttcacga tccagctctg ccgttttgcg ggcctcgccg gtctgtgggt
     5641 tcacgatttc atccaccgac aggattgccc gctgcaccct gacctgcgtc gcccggtccg
     5701 ggcagatgta ccagatttca tcgatgccgt tagctttgcg gttgaacaga tacccggaca
     5761 ccacttcctg ataccggcga cggctcttga cggtgcgctc cacctcaaac gccaccgttt
     5821 taccgtccag ctccgccagg gcgtccggca ctttcatgcc tttaagactc agacggtgca
     5881 gaatgcgcca gccggtggcc cctctggctt ccatatcaag gcgcagggat tgcacggcca
     5941 gccggtgcgg cagatgtgct gccgacaccc ttgacggctg aaacggaata acatccgcca
     6001 gcggttcgtc ctcgtcgaac gagaacgcca cgccatgcgg cgtcagcccc cacacgctga
     6061 cctgacggcc gtccaccgga tatttggtcc ggaccagcag ccctttcgcc accaggctgt
     6121 cgaggaagcg gtagaccggc tgcaccgccg caatccccag ccgccgcgca accagctccg
     6181 ccgtggaaca cagctcttcc cgcaaccagc gcagggtcag ccgggttttc tccgcgttcc
     6241 gcgcctgtcg tttcgcgtgg ctgacgttgt acagctccag cgccatcagt aaatccccgc
     6301 aaaatcatca tccgctttcg gcccgccgcc gtcggccggg ggccggaact ccgccagggg
     6361 atcggttttc gccagcaccg ggaaacgctg caacgggatt tcccggcgct caacccgcag
     6421 cggactgctg aacgccatcc gggccacacc cagcccggtc atgaccgcga acccgtccgg
     6481 caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc
     6541 cccgctgata tgctccacgt tccccgcctc ccggctggca cggcggcgct ccacgtccac
     6601 cctgatttcc ccggtctgcc ccgccgccca ctgcgcggac tccaggctgg ccgcccggta
     6661 aaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaattcag cgggcagatc
     6721 ctgcccgcac tgcccgaaat cgcccagcga ctggtgcgcg agcaacagat tgcagtcccg
     6781 gctgcgcagc gtccccagtg cgttcagtac atacttcgac agcagatatt taatttcatc
     6841 gagcatcacg ctgacgtgtg gccactcccg gaactcgtcc cgcgcaatga tgatttgcgc
     6901 gcagcgggca aacagcattt tttgcacacg gatcaccgcc tcgtcgtcca tcgagccgat
     6961 aacgtacagg cagccgccgc cgttgataat ccccgccaca tcgatgccac tgtccgtctg
     7021 caggacgctc aggtcagcga cgttttccag cctagtgatc aaccccttca cgtccttttt
     7081 cagggcctcc ggcagcgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg
     7141 aaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac
     7201 gtcgccggtc tcgcccaggt taagcccggc aatcagcagg tttttcagcg cgtactggtc
     7261 gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa
     7321 gtccggacag tgcgcccgga agacgtgcgg cgcccatgcg tcccctttgg ggtcgaagta
     7381 caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct
     7441 cttgccgctc ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg
     7501 aaccagcacc ggcttgccct gctcatcctt acccagaaac accgcctgct cctgccgggc
     7561 ctgctccgag gaaaaatcgg ccagcgcatt aaccggtacc ggctccccga aatgcaggtg
     7621 gcgcacgtcg gtcatctctc ctgtccgctg gctgctgcgc cggctctcct gattcagttt
     7681 cgcgttcacc gcctcgtcag cagccgcaaa atgaccgtca agcagcagga acgccagcag
     7741 ccgcgccaac acaatcccga cagccagccc gcccgcgcac caggcaatcc cgaacaggtt
     7801 agccggctgc agcaggtaat aatctgcgtt tgcgtagagg tccggacgca ggacggtttc
     7861 tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggccgc
     7921 gccggtcagc cagtacgcgt agttccagac atgtcgacgt gccctgacca tccgcccggg
     7981 cgaatccagc gaggccagca ccgaaaatgg cgctggccgt ttattatcgc gcttatagcg
     8041 atcggcatac tcccgtcgct ccttatgact cagccccaca aaacgcatcc gtccggccca
     8101 aagttcagcc agaccgaaaa acaggccgat aactaggaaa gccccggcca gaccgtaata
     8161 ctcctggtac agccagctga cccacggata aatctgccgg gccagctccg gaatgtgcca
     8221 cttccaggca agcgaatccg tattaaacat gttctctctc cttcagacga tggtttgccg
     8281 gcgtgatgtg cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg
     8341 gtcacgtccg tggtgattgc gttcagcttt caccacggag gccagcctct ttccgcccca
     8401 tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag
     8461 gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga
     8521 gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgccaggtg cgtccagcac
     8581 cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt
     8641 ggccggcgtc cagccggacg gagccagaaa gagggggaga gaccgtgcgt cggtggcgtc
     8701 cagccagcgt aagcagggga tatccccctg aggtcagcca cgccctcgcg gggatgacct
     8761 gtatttctcc gtaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa
     8821 aaaccgatat gattcaccta tcctggtaat ttattatcag ggaatataaa gacttaatca
     8881 ggggacaata tgccacatca gcgcgtccgg tgtccgtctt gtaggtgccg gggaaattgc
     8941 gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga ctgcggcggg cgctgcggac
     9001 acatgcaaag ttacccacag attccgtgaa taagcagggg actaacatgt gaggcaaaac
     9061 ggcaaggcca cgcaggtggc gtttttccat aggctccgcc ctcctgactg agttcacata
     9121 aacagacgct ttttcggtgc atctgtggga gccggtggct caaccatgaa tcagtcagga
     9181 gtggcgaaac ccgacaggac ttaaagatca tcaccgtttc cgatgaatcg ctccctcttg
     9241 cgctctcctg ttccaaccct gccgtttccc ggatacctgt tccgcctttc tccc
//
