LOCUS       CP128714                5251 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain KPN1402 plasmid pKPN1402_5, complete
            sequence.
ACCESSION   CP128714
VERSION     CP128714.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878161
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.942908044084x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:43:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,661
            CDSs (total)                      :: 5,536
            Genes (coding)                    :: 5,372
            CDSs (with protein)               :: 5,372
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 79 of 164
            Pseudo Genes (incomplete)         :: 87 of 164
            Pseudo Genes (internal stop)      :: 26 of 164
            Pseudo Genes (multiple problems)  :: 25 of 164
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN1402"
                     /isolation_source="bile"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN1402_5"
                     /geo_loc_name="USA:Houston"
                     /lat_lon="29.710689 N 95.400586 W"
                     /collection_date="2014-04"
                     /collected_by="Houston Methodist Hospital"
     gene            complement(join(5127..5251,1..37))
                     /locus_tag="QU739_28275"
     CDS             complement(join(5127..5251,1..37))
                     /locus_tag="QU739_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WJT52813.1"
                     /translation="MHEPPVQSDCCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="replication initiation protein"
     gene            674..994
                     /locus_tag="QU739_28280"
     CDS             674..994
                     /locus_tag="QU739_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WJT52814.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1203..1634
                     /locus_tag="QU739_28285"
                     /pseudo=""
     CDS             1203..1634
                     /locus_tag="QU739_28285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1672..2157
                     /locus_tag="QU739_28290"
     CDS             1672..2157
                     /locus_tag="QU739_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WJT52815.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            2161..2373
                     /locus_tag="QU739_28295"
     CDS             2161..2373
                     /locus_tag="QU739_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WJT52816.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3006..4361
                     /locus_tag="QU739_28300"
     CDS             3006..4361
                     /locus_tag="QU739_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005524 - ATP binding [Evidence IEA]; GO_function:
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WJT52811.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="AAA family ATPase"
     gene            4354..4974
                     /locus_tag="QU739_28305"
     CDS             4354..4974
                     /locus_tag="QU739_28305"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WJT52812.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVIYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     Typing          388..560
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(2..441)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.796"
                     /coverage="85.0"
                     /evalue="0.0"
     Typing          985..2489
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
       61 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
      121 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac
      181 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
      241 tgatgcttgt caggggggcg gagcctatgg aaaaacggca gcggcgcatc ctttttctgc
      301 cccgtttgtc atgccatatg ccttcgctct tctccgtgtg caggtaacgc ccgccgcagc
      361 cgaacggcag gagcggcagc gactgagtga gcgaggaggc gtcatatcct ctgaacttcc
      421 attttctgcc ttacgcccgc agctgcattt tcctctgtca catgaagcac tttcctgatt
      481 accgcactca tgccaacata ataagccagt atacactccg ctagcgctac gtgactggtt
      541 cagggctgcg ccccgaaacc cgctaaaagc gactgacgcg cctgcggctt gtccaacacc
      601 gtgccgaccg ggaaacattt cccgcccgtc ccggtgttat caggaggcta ctctggcaga
      661 caaacgcaac aagatgctca cgatgtgggt gacggaagac gaacaccggc ggctgctcga
      721 gcggtgcgac ggcaagcagc tggcggcgtg gatgcgtcag acctgcctcg atgagaagcc
      781 ggcgcgtgcc ggcagactgc cgtcgatatc gccggcgctg ctgcgccagc ttgccggtat
      841 ggggaacaac ctgaaccaga tcgcccggaa agtgaatgcc ggtggtgccg gtcatgaccg
      901 ggtgcagatt gtggccgcac tgatggcaat cgacgcggga ctggagcggc tgcggcatgc
      961 ggtgctggaa aaaggggggg atgatgatcg ttaaatttca cccccggggg cgcggtggtg
     1021 gtgccggtcc ggtggattac ctgctgggaa aagaccggca gcgcgagggg gcgaccgtgc
     1081 tgcagggaaa accggaggaa gtccgggagc ttatcgatgc ctcgccgtat gcgaaaaagt
     1141 acacctccgg cgtgctgtcg ttcgcggagg ctgaactgcc gcccgggcag cgggagcaga
     1201 tcatggccag ctttgagcgg gttctgatgc ccgggctcga taaagaccag tacagcattc
     1261 tgtgggtgga gcacaccgac aaggggcggc tggagctgaa tttcctgatc cccaacactg
     1321 aactgctgac cgggaaacgc ctgcagccgt actacgatcg cgccgaccgg ccgcgcatcg
     1381 atgcctggca gaccgtggtg aacggcaggc tggggctgca tgatccaaac gccccggaga
     1441 accgacggtt actggtcacg ccgtccgcac tgccggaaac gaagctggaa gccgctcagg
     1501 cgattacacg tgggttgctg gccttagcct catccgggga gctcaaaacg cgcgaggacg
     1561 tcacaggggc gctcacagcg gcaggttttg aggtggtccg caccacaaaa aacagtatca
     1621 gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatt tatgagcagt
     1681 cttttagcgc tggcgaagga cttggagcag caatcgaaag cgcagcagca gagtaccggc
     1741 gagatgctga aagccgcatt cagcgagcac gagcagtctg taaaagcgga actgagcgca
     1801 agcgcgaaga gaatcagcga cgccatcagc gcccacgagc agggtatgac cgcggcgatg
     1861 cagtcgaacc ggctgagcgt gctgcgtatg gtcggccgga cgtggctgac catcaccctg
     1921 gtctcggtac tgctgatcgc cacgagcggg agcattctgt ggtggcaggg gcagcagatc
     1981 accgggaatt accagactat ccgggcgcag gagcggacgc aggccatgct gtcggagaag
     2041 aaccacggcg tgcagctgtc gctctgcggg gagaagaaac tgtcctgcgt caaagtgaac
     2101 ccgaaagcgg gagcttacgg ggaggaaggg aactggatgg tactggagag gaaatagccc
     2161 atgacggaac tggaaaaaca gttgctgacc gcattagagc agctacatca ggactactcg
     2221 caaaggctgg acgagtggga gagcgccttc gcggaatggc agaagatgtc tggtcttatg
     2281 caacgggaga acgcggcgct gaacgagcgc gtcacgcgct tgagtcagca ggtggcgaac
     2341 ttgagcaggc agttacagcg tttgagccag tagtgcagcg gcatgaaatg gcggtggccg
     2401 ccgaacgggc atacgagcag aaacagcatg aaaaagagct ggcggaaacc cgttcccggc
     2461 agcggagcta tgacggtccg acgctgggct gactgcgcgc atccagtgac aaaccccaag
     2521 aagaagggct cataaatttc ccgctaaata cggtcgtgat gtttccatca cagcgtatac
     2581 aacggaggga ttatgagact gtttaacgtg attgagacgg tgctgaattg cctgtacatg
     2641 gcgctggaga tctgggactg gctggagaag cattttcctg tttttaaggc gttcatccgg
     2701 attctggcct gaaatcagac cgtatgcatc tggctgcatg accatgcagc cgggtaaaat
     2761 ttatctgtca cgaacggggc ggtgtatcgg gtggtttgtt gccggtttta cctgactgcc
     2821 gccccgtttg tggcgaacct gtccggagcg gtgcgggcaa cagatgttat ggtaaatagc
     2881 gtgtaattat aaatagcaca acgtttttta aggaagatgt tgttccaaaa ttaaaacata
     2941 taggatatct ttcctatgat tgatttttcg cttttttggc gaaaaatccc cgttaggagg
     3001 tatgcatgtt tgtttatttg actattgaaa attttaggtc ggttaaggaa aaatcgacat
     3061 taaacttgag cgcatccggt tctagtaacc atttaacaac tcatatctat aaaaatgatg
     3121 gaatgaatgt tggaacgctc atgagtgctg ggatttatgg tgctaatgcg tctggtaaat
     3181 ctaacgttct catggctttt gaagctataa aatttatcgc aacagaaagc ggtgatttga
     3241 aagaaggtaa acggattccc tgctatgagc catatgctct ttcttcggct acgaatgagg
     3301 ctcctgtaaa gtttgaggct gaattttatt ctgttgaggg gaatcggttc agttatgaag
     3361 ttgcttttat taaaaatcgg attttatttg aatcattaga ctattatcca tctagggtta
     3421 aagcaaattt atttactaga gatgagagcg acacctggga aacaatcaag tttggtgggc
     3481 attataaagg aggtattaaa aaaataccat tcttccctaa taattcatac ttagcgaaag
     3541 caggtaataa tgccgcatca ccagacataa ttaaagaagc ttataacttt tttagaaagg
     3601 gaattagaca tataggttta aatgaaaaga taagaatatc ttcttttggg gagagggagg
     3661 acgtcgtaaa tgaaacggcc aaggttttat gcatggttga cactggtgtt tccggtatat
     3721 ctgtacggga ggttgaggcg gaaataccaa taaaaatgga tgaggaaatc cctcaagaaa
     3781 taaaggagat aattgaagaa gattataaat atcgttatat gttctctcat cctactgatg
     3841 aaggggggag tgtttctttc cctatgggga gagagtctga aggcacccaa aaattatttg
     3901 aaatcatacc tctaattaga tctgcgttta atgatagtat ggttgtaatt gttgatgagt
     3961 tagataatag cttgcacccg catattgctg acttgattgt taagttattt aatgatcctg
     4021 atgtcaataa aaaaggctca caacttgtat tctcaaccca taatatgcag ttaatggctc
     4081 cagagaaaat gagaagagat cagatatggt tttgtgaaaa aaataaaggt gcgtcgtctc
     4141 tttatagctt agatgatttt gataagaaaa agataaaaac aacaacacct tacgcagctt
     4201 ggtatgatga gggtaggttt ggtggggttc ccgacataaa ctacttaaag gtagcctcat
     4261 ttatatctgg tgatatatcc ttggtaatgc cggatattga cgtcaaagaa ttgtctgatg
     4321 gtttttttga agagtttgac ggggatcttt ctgatgagta aaaaacatgg aaataataag
     4381 aaagtcccac aagtaatgta tatatactgt gagggtgaga aaaccgaacc attatacctt
     4441 gaggcttata tttcagacag atctaaaaga actttaagtg tatttaaaat acctaaaaca
     4501 aggaaaaata cacctgagca gttggtggat gaggcgataa agaaaaagaa ttcatcatct
     4561 tccgcagaag gtgacgtctt ttgggttatt tatgatcaag agcatctgac gccttcgtct
     4621 atttcatgtc atcaaaaggc atggaataaa gctaataggc atgggataaa tatagcaata
     4681 tcctgtgttt gttttgaatt atggttattg ttgcatttcg gcttcacaac caaagatttt
     4741 tcgtcgtatg ataatttgat gtctagctcc ccgctaaaaa aattaataac taactatgat
     4801 aaaggtagta aactaactta ccttgctata agagagagga ttggtactgc gaaagataac
     4861 gcaagaaagc tagatattta cgctaaaact aattataaaa acacgcacga accttatcta
     4921 tttccatgtt atacatcatt ccatgagcta cttaaggcga ttgacgactt ttgaagtaat
     4981 ccattgacca tggcttatta caggctgtaa tctatgaaaa ataaaaacca ccgctaccaa
     5041 cggtggtttt tggaaggtac atgagctacc aacttatgta ccgggtaact ggcttagagg
     5101 gagcgcagtc accaaaaatt gtcctttcag tttagcctta actggcgcag aacttcaaga
     5161 ctcacttcct ctaatccgtt accaatggct gctgccagtg gcgatgaacc gtgtctttcc
     5221 gggttggact caagatgata gttaccggag a
//
